Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006165

Physical Location & Seq

Genomic Coordinates Forward (+)

8123939 .. 8142120

Gene Structure 18182 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1047800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGGACTCGGCGGCAATGGATGAACAGCCCGCTCACGTCAAAGACTTGGCGGCCTTTCTCATACGCCACCACTCAGACCAGCTTCGCGCCATCGCTCTTTCCCGAGACCCCAAGCTTCATTACCCTCTCTACGTCAGTTTCGCAGAGCTTATGGATGCCGATCCTCCGCTTTCGCATCTCGTTTTCTCTCAACCCATTGAGTACTTGCGCTTTTTCGATGAGGCCGCTCTTTGGGCTCATAAAGTCACATTGAGGGATTTGGATGGTTTTGATAAAGGGATAAAGAAGGAATTCATTCATGTCCGGATTGATATCAGTGGTTCGCCGCTCGAATGCCCTGTAGAGACCTTTCCGAGCATTGGCCGTGTGAGAGTTAAGCATAGAGGGATTCTGCTCACCCTTAAAGGGACGGTGATTCGGTCTGGAGCAATCAAGATGTATGAAGGAGAGAGGAAGTATATGTGTCAGAAATGCAAGCACGAATATCTGGTTTATCCCGAACTGGAATCGAGAAACTCCATAAAACTACCATCATATTGCCCTTCTCAGAGGTCTAACCCCTGTGAAGGAAAAAAGTTCACGCATTTGGAAGGTTCTATTACATGCCATGATTATCAGGAAATCAAAATCCAGGAGAGTACACATGTATTGGGTGTTGGGGCAATTCCTCGTTCAATCCCTGTCATTTTAAAGGACGATCTTGTTGATATCATTAAAGCTGGAGATGGTGTTATTGTTACTGGGATATTAACTGCAAAGTGGTCTCCAGACTTAAAAGATGTGCGCTGTGACCTCGATCCTATATTAATTGCTAATTATATTAGGAGAACTAATGCGCTAAAGTCGGAAATTGATATACCTGATGATGCTATAGTGAAATTTAAGCAGTTCTGGTCAGACTTCAAAGATAGCCCTTTGAAAGGGAGGAATGCCATTCTACGAGGTATTTGCCCACAAGTATTTGGACTCTTTACTGTAAAGCTTGCAGTGGCATTAACACTTATTGGAGGTGTGCAACATGTGGATGTTTCTGGGACAAAGGTTCGAGGAGAGTCTCATTTGCTTTTGGTTGGTGATCCAGGTACAGGAAAATCTCAGTTCTTGAAGTTTGCTGCAAAGTTGAGCAACCGATCGGTTATTACAACCGGATTAGGAAGCACTAGTGCTGGATTAACAGTCACTGCAGTCAAGGATGGAGGGGAATGGATGCTGGAAGCTGGAGCCCTTGTTTTAGCAGATGGAGGGCTTTGTTGTATAGACGAATTTGACAGCATGAGAGAACATGATAGGGCAACCATACATGAAGCAATGGAGCAGCAGACTATAAGTGTTGCGAAGGCGGGTCTTGTGACAACTCTCAGTACTAAAACAATCGTTTTTGGTGCAACAAATCCCAAGGGACAGTATGATCCTGATCAATCGCTGTCAATCAATACGGCGCTGTCTGGTCCCTTATTGAGCAGATTTGATATAGTCCTTGTACTCTTGGATACAAAGAATCCTGAATGGGATGGGGTTGTGTCATCTCACATTCTTTCTGAGGGAGAACCAGACAAAGGCAACCATGAGGAAGATTTAGCAAACATCTGGCCATTTTCGATGCTGCGTAGATATATTCACTTTGTAAAAGGGTACTTCAGACCAGTCCTCACAAAAGAAGCTGAGATGGTCATCTCAAACTATTATCAACTGCAAAGAAGGTCTGCAACCGGCAATGCAGCTAGGACAACTGTGCGCATGCTTGAAAGTTTGATACGCCTAGCTCAAGCACACGCAAGACTGATGTTCAGAAATGAGGTTACACGGTTAGATGCCATAACAGCCATTTTATGCATTGAATCATCCATGACTACCTCGGCCATAGTAGACAGTGTTGGAAATGCTCTGCATTCAAATTTCGCTGAAAACCCTGATCAAGAATATGCCAAGCAAGAAAGGCTGATCCTTGAGAAGCTAAGATCATTCGATGAGTTCCCAGACACAAATAGCATGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0005575 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0032991 GO:0033554 GO:0034641 GO:0042555 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:0097362 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM MCM_OB

Protein Analysis

666

Amino Acids

73.95

Weight (kDa)

6.33

Isoelectric Point (pI)

35.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM9_N	PF26066	15 - 103	1.6e-10	MCM9, N-terminal domain
MCM_OB	PF17207	123 - 251	3.9e-22	MCM OB domain
MCM	PF00493	310 - 513	3e-72	MCM P-loop domain
AAA_5	PF07728	353 - 469	8.6e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	534 - 615	1.1e-18	MCM AAA-lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006165)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G14050 AT2G14050
fragaria_vesca	FvH4_2g10130 FvH4_2g10130 FvH4_2g10130 FvH4_2g10130 FvH4_2g10130 FvH4_2g10130 FvH4_2g10130
malus_domestica	MD05G1047800.v1.1
prunus_persica	Prupe.8G065100_v2.0.a1 Prupe.8G065100_v2.0.a1 Prupe.8G065100_v2.0.a1 Prupe.8G065100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0267231
rosa_laevigata	RLG00000014019
rosa_multiflora	Rmu_co8135206.1_g000001 Rmu_ssc0000388.1_g000049
rosa_roxburghii	Rroxscaffold_7G00200990
rosa_rugosa	Rorug06G0030500.1 Rorug06G0030600 Rorug06G0030700
rosa_samantha	Rh6AG152600 Rh6BG152700 Rh6CG148600 Rh6DG136900
rosa_wichuraiana	Rw6G013150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1736
AccBSI	CCGCTC	3 cut(s)	32, 227, 328
AccI	GTMKAC	1 cut(s)	1866
AccII	CGCG	1 cut(s)	87
AccIII	TCCGGA	1 cut(s)	303
AciI	CCGC	7 cut(s)	11, 30, 50, 167, 225, 326, 1340
AclWI	GGATC	5 cut(s)	155, 791, 1070, 1403, 1936
AcoI	YGGCCR	3 cut(s)	361, 1589, 1857
AcsI	RAATTY	4 cut(s)	290, 878, 1262, 1894
AcuI	CTGAAG	1 cut(s)	1621
AfaI	GTAC	6 cut(s)	203, 640, 1084, 1363, 1482, 1634
AfiI	CCNNNNNNNGG	7 cut(s)	231, 359, 405, 566, 920, 1085, 1556
AflIII	ACRYGT	2 cut(s)	643, 1018
AgsI	TTSAA	7 cut(s)	675, 904, 919, 1105, 1745, 1838, 1893
AhlI	ACTAGT	1 cut(s)	1160
AjiI	CACGTC	1 cut(s)	37
AjnI	CCWGG	2 cut(s)	630, 1078
Alw26I	GTCTC	4 cut(s)	99, 338, 768, 1059
AlwI	GGATC	5 cut(s)	155, 791, 1070, 1403, 1936
AlwNI	CAGNNNCTG	1 cut(s)	1181
Ama87I	CYCGRG	1 cut(s)	102
Aor13HI	TCCGGA	1 cut(s)	303
AoxI	GGCC	5 cut(s)	51, 222, 361, 1589, 1857
ApeKI	GCWGC	4 cut(s)	1112, 1315, 1603, 1718
ApoI	RAATTY	4 cut(s)	290, 878, 1262, 1894
AseI	ATTAAT	1 cut(s)	806
Asp700I	GAANNNNTTC	2 cut(s)	290, 294
AspLEI	GCGC	6 cut(s)	89, 210, 786, 838, 1441, 1737
AspS9I	GGNCC	1 cut(s)	1448
AsuHPI	GGTGA	3 cut(s)	388, 424, 1085
AvaI	CYCGRG	1 cut(s)	102
AvaII	GGWCC	1 cut(s)	1448
BalI	TGGCCA	1 cut(s)	1591
BanII	GRGCYC	2 cut(s)	238, 1225
BbvI	GCAGC	4 cut(s)	1099, 1327, 1590, 1730
BccI	CCATC	8 cut(s)	98, 257, 538, 719, 1187, 1232, 1505, 1660
BceAI	ACGGC	2 cut(s)	348, 1452
BcgI	CGANNNNNNTGC	2 cut(s)	930, 964
BciT130I	CCWGG	2 cut(s)	632, 1080
BciVI	GTATCC	1 cut(s)	1483
BclI	TGATCA	2 cut(s)	1414, 1912
BcoDI	GTCTC	4 cut(s)	99, 338, 768, 1059
BcuI	ACTAGT	1 cut(s)	1160
BfaI	CTAG	3 cut(s)	1161, 1722, 1760
BfmI	CTRYAG	3 cut(s)	339, 870, 1182
BfoI	RGCGCY	1 cut(s)	1442
BfuI	GTATCC	1 cut(s)	1483
BisI	GCNGC	8 cut(s)	12, 51, 225, 326, 1113, 1316, 1604, 1719
BlsI	GCNGC	8 cut(s)	13, 52, 226, 327, 1114, 1317, 1605, 1720
BmcAI	AGTACT	2 cut(s)	203, 1363
Bme1390I	CCNGG	2 cut(s)	632, 1080
Bme18I	GGWCC	1 cut(s)	1448
BmeT110I	CYCGRG	1 cut(s)	102
BmgBI	CACGTC	1 cut(s)	37
BmgT120I	GGNCC	1 cut(s)	1448
BmiI	GGNNCC	2 cut(s)	1222, 1450
BmrFI	CCNGG	2 cut(s)	632, 1080
BmrI	ACTGGG	1 cut(s)	750
BmsI	GCATC	6 cut(s)	145, 184, 856, 1197, 1590, 1801
BmuI	ACTGGG	1 cut(s)	750
BpmI	CTGGAG	4 cut(s)	444, 741, 750, 1239
BpuEI	CTTGAG	2 cut(s)	1749, 1967
BsaBI	GATNNNNATC	4 cut(s)	159, 311, 1413, 1670
BsaI	GGTCTC	3 cut(s)	99, 338, 768
BsaJI	CCNNGG	2 cut(s)	1395, 1854
BsaWI	WCCGGW	2 cut(s)	303, 1145
BsaXI	ACNNNNNCTCC	4 cut(s)	714, 744, 1212, 1242
Bsc4I	CCNNNNNNNGG	7 cut(s)	231, 359, 405, 566, 920, 1085, 1556
Bse118I	RCCGGY	1 cut(s)	1709
Bse1I	ACTGG	3 cut(s)	507, 745, 1643
Bse3DI	GCAATG	3 cut(s)	21, 1314, 1720
Bse8I	GATNNNNATC	4 cut(s)	159, 311, 1413, 1670
BseAI	TCCGGA	1 cut(s)	303
BseBI	CCWGG	2 cut(s)	632, 1080
BseDI	CCNNGG	2 cut(s)	1395, 1854
BseGI	GGATG	8 cut(s)	25, 160, 268, 1030, 1198, 1212, 1516, 1841
BseJI	GATNNNNATC	4 cut(s)	159, 311, 1413, 1670
BseLI	CCNNNNNNNGG	7 cut(s)	231, 359, 405, 566, 920, 1085, 1556
BseMI	GCAATG	3 cut(s)	21, 1314, 1720
BseMII	CTCAG	6 cut(s)	87, 560, 1109, 1372, 1530, 1653
BseNI	ACTGG	3 cut(s)	507, 745, 1643
BseRI	GAGGAG	1 cut(s)	1062
BseXI	GCAGC	4 cut(s)	1099, 1327, 1590, 1730
Bsh1236I	CGCG	1 cut(s)	87
Bsh1285I	CGRYCG	1 cut(s)	1133
BshFI	GGCC	5 cut(s)	53, 224, 363, 1591, 1859
BsiEI	CGRYCG	1 cut(s)	1133
BsiHKCI	CYCGRG	1 cut(s)	102
BsiSI	CCGG	3 cut(s)	304, 1146, 1710
BslFI	GGGAC	4 cut(s)	421, 1048, 1413, 1434
BslI	CCNNNNNNNGG	7 cut(s)	231, 359, 405, 566, 920, 1085, 1556
BsmAI	GTCTC	4 cut(s)	99, 338, 768, 1059
BsmFI	GGGAC	4 cut(s)	421, 1048, 1413, 1434
BsmI	GAATGC	3 cut(s)	338, 934, 1888
BsnI	GGCC	5 cut(s)	53, 224, 363, 1591, 1859
Bso31I	GGTCTC	3 cut(s)	99, 338, 768
BsoBI	CYCGRG	1 cut(s)	102
Bsp1286I	GDGCHC	2 cut(s)	238, 1225
Bsp13I	TCCGGA	1 cut(s)	303
BspACI	CCGC	7 cut(s)	11, 30, 50, 167, 225, 326, 1340
BspANI	GGCC	5 cut(s)	53, 224, 363, 1591, 1859
BspCNI	CTCAG	6 cut(s)	86, 559, 1108, 1371, 1531, 1654
BspEI	TCCGGA	1 cut(s)	303
BspFNI	CGCG	1 cut(s)	87
BspLI	GGNNCC	2 cut(s)	1222, 1450
BspMAI	CTGCAG	1 cut(s)	1186
BspPI	GGATC	5 cut(s)	155, 791, 1070, 1403, 1936
BspTNI	GGTCTC	3 cut(s)	99, 338, 768
BsrBI	CCGCTC	3 cut(s)	32, 227, 328
BsrDI	GCAATG	3 cut(s)	21, 1314, 1720
BsrFI	RCCGGY	1 cut(s)	1709
BsrI	ACTGG	3 cut(s)	507, 745, 1643
BssAI	RCCGGY	1 cut(s)	1709
BssECI	CCNNGG	2 cut(s)	1395, 1854
BssT1I	CCWWGG	1 cut(s)	1395
Bst2UI	CCWGG	2 cut(s)	632, 1080
Bst4CI	ACNGT	7 cut(s)	412, 976, 1177, 1404, 1732, 1806, 1871
BstC8I	GCNNGC	5 cut(s)	30, 476, 984, 1739, 1991
BstDEI	CTNAG	7 cut(s)	73, 546, 1095, 1358, 1539, 1662, 1955
BstENI	CCTNNNNNAGG	1 cut(s)	918
BstF5I	GGATG	8 cut(s)	25, 160, 268, 1030, 1198, 1212, 1516, 1841
BstFNI	CGCG	1 cut(s)	87
BstH2I	RGCGCY	1 cut(s)	1442
BstHHI	GCGC	6 cut(s)	89, 210, 786, 838, 1441, 1737
BstMAI	GTCTC	4 cut(s)	99, 338, 768, 1059
BstMCI	CGRYCG	1 cut(s)	1133
BstMWI	GCNNNNNNNGC	3 cut(s)	233, 1820, 1936
BstNI	CCWGG	2 cut(s)	632, 1080
BstNSI	RCATGY	5 cut(s)	606, 647, 1022, 1741, 1993
BstSCI	CCNGG	2 cut(s)	630, 1078
BstSFI	CTRYAG	3 cut(s)	339, 870, 1182
BstUI	CGCG	1 cut(s)	87
BstV1I	GCAGC	4 cut(s)	1099, 1327, 1590, 1730
BsuI	GTATCC	1 cut(s)	1483
BsuRI	GGCC	5 cut(s)	53, 224, 363, 1591, 1859
BtgZI	GCGATG	1 cut(s)	76
BtrI	CACGTC	1 cut(s)	37
BtsCI	GGATG	8 cut(s)	25, 160, 268, 1030, 1198, 1212, 1516, 1841
BtsI	GCAGTG	2 cut(s)	993, 1179
BtsIMutI	CAGTG	4 cut(s)	322, 993, 1179, 1876
Cac8I	GCNNGC	5 cut(s)	30, 476, 984, 1739, 1991
CaiI	CAGNNNCTG	1 cut(s)	1181
CfoI	GCGC	6 cut(s)	89, 210, 786, 838, 1441, 1737
Cfr10I	RCCGGY	1 cut(s)	1709
Cfr13I	GGNCC	1 cut(s)	1448
Csp6I	GTAC	6 cut(s)	202, 639, 1083, 1362, 1481, 1633
CviQI	GTAC	6 cut(s)	202, 639, 1083, 1362, 1481, 1633
DdeI	CTNAG	7 cut(s)	73, 546, 1095, 1358, 1539, 1662, 1955
DraI	TTTAAA	1 cut(s)	690
EaeI	YGGCCR	3 cut(s)	361, 1589, 1857
Eco130I	CCWWGG	1 cut(s)	1395
Eco24I	GRGCYC	2 cut(s)	238, 1225
Eco31I	GGTCTC	3 cut(s)	99, 338, 768
Eco32I	GATATC	2 cut(s)	313, 709
Eco47I	GGWCC	1 cut(s)	1448
Eco57I	CTGAAG	1 cut(s)	1621
Eco88I	CYCGRG	1 cut(s)	102
EcoNI	CCTNNNNNAGG	1 cut(s)	918
EcoRI	GAATTC	1 cut(s)	290
EcoRII	CCWGG	2 cut(s)	630, 1078
EcoRV	GATATC	2 cut(s)	313, 709
EcoT14I	CCWWGG	1 cut(s)	1395
EcoT22I	ATGCAT	1 cut(s)	1835
EcoT38I	GRGCYC	2 cut(s)	238, 1225
ErhI	CCWWGG	1 cut(s)	1395
FaqI	GGGAC	4 cut(s)	421, 1048, 1413, 1434
FauI	CCCGC	2 cut(s)	37, 1333
FbaI	TGATCA	2 cut(s)	1414, 1912
FblI	GTMKAC	1 cut(s)	1866
Fnu4HI	GCNGC	8 cut(s)	12, 51, 225, 326, 1113, 1316, 1604, 1719
FokI	GGATG	8 cut(s)	32, 167, 275, 1037, 1205, 1219, 1523, 1828
FriOI	GRGCYC	2 cut(s)	238, 1225
Fsp4HI	GCNGC	8 cut(s)	12, 51, 225, 326, 1113, 1316, 1604, 1719
FspAI	RTGCGCAY	1 cut(s)	1736
FspBI	CTAG	3 cut(s)	1161, 1722, 1760
FspI	TGCGCA	1 cut(s)	1736
GlaI	GCGC	6 cut(s)	88, 209, 785, 837, 1440, 1736
GluI	GCNGC	8 cut(s)	12, 51, 225, 326, 1113, 1316, 1604, 1719
GsuI	CTGGAG	4 cut(s)	444, 741, 750, 1239
HaeII	RGCGCY	1 cut(s)	1442
HaeIII	GGCC	5 cut(s)	53, 224, 363, 1591, 1859
HapII	CCGG	3 cut(s)	304, 1146, 1710
HhaI	GCGC	6 cut(s)	89, 210, 786, 838, 1441, 1737
Hin6I	GCGC	6 cut(s)	87, 208, 784, 836, 1439, 1735
HinP1I	GCGC	6 cut(s)	87, 208, 784, 836, 1439, 1735
HindIII	AAGCTT	2 cut(s)	113, 980
HinfI	GANTC	8 cut(s)	5, 388, 415, 506, 966, 1052, 1498, 1838
HpaII	CCGG	3 cut(s)	304, 1146, 1710
HphI	GGTGA	3 cut(s)	388, 424, 1085
Hpy166II	GTNNAC	3 cut(s)	579, 641, 1867
Hpy188I	TCNGA	9 cut(s)	76, 354, 468, 549, 847, 898, 1540, 1640, 1790
Hpy8I	GTNNAC	3 cut(s)	579, 641, 1867
HpyAV	CCTTC	7 cut(s)	280, 437, 552, 560, 584, 1330, 1692
HpyCH4III	ACNGT	7 cut(s)	412, 976, 1177, 1404, 1732, 1806, 1871
HpyCH4IV	ACGT	2 cut(s)	36, 132
HpyF10VI	GCNNNNNNNGC	3 cut(s)	233, 1820, 1936
HpyF3I	CTNAG	7 cut(s)	73, 546, 1095, 1358, 1539, 1662, 1955
HpySE526I	ACGT	2 cut(s)	36, 132
HspAI	GCGC	6 cut(s)	87, 208, 784, 836, 1439, 1735
Kpn2I	TCCGGA	1 cut(s)	303
Ksp22I	TGATCA	2 cut(s)	1414, 1912
LmnI	GCTCC	3 cut(s)	425, 1220, 1312
Lsp1109I	GCAGC	4 cut(s)	1099, 1327, 1590, 1730
LweI	GCATC	6 cut(s)	145, 184, 856, 1197, 1590, 1801
MaeI	CTAG	3 cut(s)	1161, 1722, 1760
MaeII	ACGT	2 cut(s)	36, 132
MaeIII	GTNAC	6 cut(s)	244, 736, 788, 1177, 1348, 1798
MbiI	CCGCTC	3 cut(s)	32, 227, 328
MboII	GAAGA	1 cut(s)	1583
MhlI	GDGCHC	2 cut(s)	238, 1225
MlsI	TGGCCA	1 cut(s)	1591
MluCI	AATT	8 cut(s)	290, 663, 807, 814, 849, 878, 1262, 1894
MluNI	TGGCCA	1 cut(s)	1591
MlyI	GAGTC	2 cut(s)	960, 1061
MmeI	TCCRAC	2 cut(s)	825, 1855
Mox20I	TGGCCA	1 cut(s)	1591
Mph1103I	ATGCAT	1 cut(s)	1835
MroI	TCCGGA	1 cut(s)	303
MroXI	GAANNNNTTC	2 cut(s)	290, 294
MscI	TGGCCA	1 cut(s)	1591
MslI	CAYNNNNRTG	1 cut(s)	1023
Msp20I	TGGCCA	1 cut(s)	1591
MspI	CCGG	3 cut(s)	304, 1146, 1710
MspR9I	CCNGG	2 cut(s)	632, 1080
Mva1269I	GAATGC	3 cut(s)	338, 934, 1888
MvaI	CCWGG	2 cut(s)	632, 1080
MvnI	CGCG	1 cut(s)	87
MwoI	GCNNNNNNNGC	3 cut(s)	233, 1820, 1936
NlaIV	GGNNCC	2 cut(s)	1222, 1450
NmeAIII	GCCGAG	1 cut(s)	1835
NmuCI	GTSAC	4 cut(s)	244, 788, 1177, 1348
NsbI	TGCGCA	1 cut(s)	1736
NsiI	ATGCAT	1 cut(s)	1835
NspI	RCATGY	5 cut(s)	606, 647, 1022, 1741, 1993
PaeI	GCATGC	2 cut(s)	1741, 1993
PciI	ACATGT	2 cut(s)	643, 1018
PctI	GAATGC	3 cut(s)	338, 934, 1888
PdmI	GAANNNNTTC	2 cut(s)	290, 294
PfeI	GAWTC	5 cut(s)	388, 415, 506, 1498, 1838
PfoI	TCCNGGA	1 cut(s)	630
PkrI	GCNGC	8 cut(s)	13, 52, 226, 327, 1114, 1317, 1605, 1720
Ple19I	CGATCG	1 cut(s)	1133
PleI	GAGTC	2 cut(s)	960, 1060
PpsI	GAGTC	2 cut(s)	960, 1060
PscI	ACATGT	2 cut(s)	643, 1018
PshBI	ATTAAT	1 cut(s)	806
Psp6I	CCWGG	2 cut(s)	630, 1078
PspGI	CCWGG	2 cut(s)	630, 1078
PspN4I	GGNNCC	2 cut(s)	1222, 1450
PspPI	GGNCC	1 cut(s)	1448
PstI	CTGCAG	1 cut(s)	1186
PstNI	CAGNNNCTG	1 cut(s)	1181
PvuI	CGATCG	1 cut(s)	1133
RsaI	GTAC	6 cut(s)	203, 640, 1084, 1363, 1482, 1634
RsaNI	GTAC	6 cut(s)	202, 639, 1083, 1362, 1481, 1633
RseI	CAYNNNNRTG	1 cut(s)	1023
SatI	GCNGC	8 cut(s)	12, 51, 225, 326, 1113, 1316, 1604, 1719
Sau96I	GGNCC	1 cut(s)	1448
ScaI	AGTACT	2 cut(s)	203, 1363
SchI	GAGTC	2 cut(s)	960, 1061
ScrFI	CCNGG	2 cut(s)	632, 1080
SduI	GDGCHC	2 cut(s)	238, 1225
SfaNI	GCATC	6 cut(s)	145, 184, 856, 1197, 1590, 1801
SfcI	CTRYAG	3 cut(s)	339, 870, 1182
SinI	GGWCC	1 cut(s)	1448
SmiMI	CAYNNNNRTG	1 cut(s)	1023
SmlI	CTYRAG	2 cut(s)	1764, 1946
SmoI	CTYRAG	2 cut(s)	1764, 1946
SpeI	ACTAGT	1 cut(s)	1160
SphI	GCATGC	2 cut(s)	1741, 1993
Sse9I	AATT	8 cut(s)	290, 663, 807, 814, 849, 878, 1262, 1894
SsiI	CCGC	7 cut(s)	11, 30, 50, 167, 225, 326, 1340
SspMI	CTAG	3 cut(s)	1161, 1722, 1760
StyD4I	CCNGG	2 cut(s)	630, 1078
StyI	CCWWGG	1 cut(s)	1395
TaaI	ACNGT	7 cut(s)	412, 976, 1177, 1404, 1732, 1806, 1871
TaiI	ACGT	2 cut(s)	39, 135
TaqI	TCGA	7 cut(s)	216, 330, 509, 795, 1045, 1598, 1965
TaqII	GACCGA	1 cut(s)	408
TasI	AATT	8 cut(s)	290, 663, 807, 814, 849, 878, 1262, 1894
TatI	WGTACW	4 cut(s)	201, 638, 1361, 1480
TauI	GCSGC	4 cut(s)	14, 53, 227, 328
TfiI	GAWTC	5 cut(s)	388, 415, 506, 1498, 1838
TscAI	CASTG	4 cut(s)	322, 993, 1186, 1876
TseFI	GTSAC	4 cut(s)	244, 788, 1177, 1348
TseI	GCWGC	4 cut(s)	1112, 1315, 1603, 1718
Tsp45I	GTSAC	4 cut(s)	244, 788, 1177, 1348
TspDTI	ATGAA	6 cut(s)	36, 107, 283, 287, 456, 1317
TspRI	CASTG	4 cut(s)	322, 993, 1186, 1876
VpaK11BI	GGWCC	1 cut(s)	1448
VspI	ATTAAT	1 cut(s)	806
XagI	CCTNNNNNAGG	1 cut(s)	918
XapI	RAATTY	4 cut(s)	290, 878, 1262, 1894
XceI	RCATGY	5 cut(s)	606, 647, 1022, 1741, 1993
XmiI	GTMKAC	1 cut(s)	1866
XmnI	GAANNNNTTC	2 cut(s)	290, 294
XspI	CTAG	3 cut(s)	1161, 1722, 1760
ZrmI	AGTACT	2 cut(s)	203, 1363
Zsp2I	ATGCAT	1 cut(s)	1835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.