MD05G1071900.v1.1

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009094
Physical Location & Seq
Forward (+)
15148994 .. 15152087
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1071900.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGGCGATCACTCTCCACTTCGGCCGAATTCCACCGTGTAACGATCTCTTCCTCTCAGAGAGCCTCGCCGGGAGAGTATCTCTCAATTGCCGGAAACCCTTCACCGTCCGATGCTCCGGCGACTCCTCTTACTCCGCCGTCGAGTCCGACTTCGACGCCAAGGTCTTCCGCAAGAACCTTACTCGAAGCAAGAACTACAACCGCAGAGGCTTCGGCCACAAAGAGGTGACACTGGAGCTCATGAACCGCGAGTACACAAGCGATATTATCAAGACGTTGAAGGAGAACGGGTTCGAGTACACCTGGGGAAACGTGAAGGTGAAGCTCGCGGAGGCGTACGGATTCTGTTGGGGCGTTGAGAGAGCCGTGCAGATTGCTTACGAGGCCAGAAAGCAGTTTCCCGATGATAAGATTTGGATCACCAACGAAATCATCCACAATCCAACGGTCAATAAGAGGTTGGAGGAGATGAAGGTGGAGAACATTCCGATAGAGGAAGGTCAGAAGCAGTTTGAGGTGGTGAACAAGGGCGATGTGGTCATTTTGCCTGCTTTCGGAGCCGGAGTGGATGAGATGCTGGACTTGAGTAACAGAGATGTTCAAATTGTTGACACTACTTGCCCCTGGGTTTCCAAGGTTTGGAATACTGTGGAGAAGCACAAGAAAGGAGATTACACTTCGATCATTCATGGGAAATATTCTCATGAGGAGACTGTGGCCACTGCTTCTTTTGCTGGGAAGTACGTTATAGTGAAAAACATGGATGAGGCAACGTATGTGTGTGACTATATTCTTGGCGGGGAACTTAATGGGTCTAGCTCAACTAAAGAGGCGTTTATGGCGAAATTTAAAAAGGCACTCTCTCCGGGGTTTGATCCAGACAGAGACCTGGTAAAAGTTGGGATTGCAAATCAGACAACAATGCTTAAGGGGGAAACTGAAGAGATTGGAAAATTAGTAGAGAAGACCATGATGCGTAAGCACGGAGTGGAAAATATAAATGACCACTTTGTGAGCTTCAACACAATATGTGATGCAACTCAGGAGCGACAAGATGCAATGTACAAGCTCGTTGAGGAGAAGCTAGATCTTTTGTTAGTCATTGGGGGGTGGAACTCAAGTAACACCTCTCACCTACAAGAGATTGCAGAAGAACGTGGAATTCCCTCGTATTGGATTGATAGTGAGAAGAGAATAGGCCCTGGAAACAGAATAGCTTATAAGCTCAATCATGGCGAGTTGGTCGAGAAAGAAAACTGGCTGCCAGAAGGTCCTGTCACTATTGGAGTGACATCTGGGGCATCTACTCCAGATAAGGTTGTTGAAGACACTCTTATCAGGGTGTTCGACCTCAAGCGTGAAGAGGTCCTGCAACTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
LYTB

Protein Analysis

461

Amino Acids

51.97

Weight (kDa)

5.65

Isoelectric Point (pI)

29.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LYTB PF02401 107 - 445 4.2e-80 LytB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
AasI GACNNNNNNGTC 1 cut(s) 161
AccB7I CCANNNNNTGG 1 cut(s) 639
AccII CGCG 2 cut(s) 249, 329
AciI CCGC 6 cut(s) 135, 169, 202, 247, 329, 798
AclWI GGATC 2 cut(s) 425, 869
AcoI YGGCCR 3 cut(s) 22, 214, 717
AcsI RAATTY 3 cut(s) 27, 845, 1161
AcuI CTGAAG 1 cut(s) 960
AcyI GRCGYC 1 cut(s) 156
AdeI CACNNNGTG 2 cut(s) 988, 1012
AfaI GTAC 5 cut(s) 254, 299, 338, 743, 1064
AfiI CCNNNNNNNGG 4 cut(s) 223, 554, 639, 1173
AflII CTTAAG 1 cut(s) 926
AgsI TTSAA 4 cut(s) 280, 602, 1021, 1325
AjnI CCWGG 4 cut(s) 302, 623, 888, 1201
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
AluBI AGCT 8 cut(s) 238, 325, 819, 1017, 1069, 1084, 1217, 1225
AluI AGCT 8 cut(s) 238, 325, 819, 1017, 1069, 1084, 1217, 1225
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 2 cut(s) 704, 879
AlwI GGATC 2 cut(s) 425, 869
AoxI GGCC 5 cut(s) 22, 214, 384, 717, 1198
ApeKI GCWGC 1 cut(s) 1261
ApoI RAATTY 3 cut(s) 27, 845, 1161
Asp700I GAANNNNTTC 2 cut(s) 98, 290
AspS9I GGNCC 3 cut(s) 1199, 1271, 1366
AsuC2I CCSGG 2 cut(s) 70, 867
AsuHPI GGTGA 6 cut(s) 94, 238, 331, 412, 532, 1124
AvaII GGWCC 2 cut(s) 1271, 1366
BalI TGGCCA 1 cut(s) 719
BanII GRGCYC 1 cut(s) 240
BbsI GAAGAC 3 cut(s) 157, 971, 1332
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 1248
BceAI ACGGC 2 cut(s) 122, 350
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 4 cut(s) 304, 625, 890, 1203
BcnI CCSGG 2 cut(s) 70, 867
BcoDI GTCTC 2 cut(s) 704, 879
BfaI CTAG 3 cut(s) 816, 1085, 1376
BfrI CTTAAG 1 cut(s) 926
BglII AGATCT 1 cut(s) 1087
BisI GCNGC 1 cut(s) 1262
BlsI GCNGC 1 cut(s) 1263
Bme1390I CCNGG 6 cut(s) 70, 304, 625, 867, 890, 1203
Bme18I GGWCC 2 cut(s) 1271, 1366
BmgT120I GGNCC 3 cut(s) 1199, 1271, 1366
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 6 cut(s) 70, 304, 625, 867, 890, 1203
BmsI GCATC 6 cut(s) 101, 564, 963, 1024, 1045, 1310
BpiI GAAGAC 3 cut(s) 157, 971, 1332
BplI GAGNNNNNCTC 4 cut(s) 64, 96, 66, 98
BpmI CTGGAG 2 cut(s) 254, 1293
BpuEI CTTGAG 3 cut(s) 604, 1102, 1337
BpuMI CCSGG 2 cut(s) 70, 867
BsaBI GATNNNNATC 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 879
BsaJI CCNNGG 7 cut(s) 159, 303, 623, 624, 633, 866, 1201
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 549, 579
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 554, 639, 1173
Bse1I ACTGG 2 cut(s) 237, 1262
Bse3DI GCAATG 1 cut(s) 1065
Bse8I GATNNNNATC 1 cut(s) 416
BseBI CCWGG 4 cut(s) 304, 625, 890, 1203
BseDI CCNNGG 7 cut(s) 159, 303, 623, 624, 633, 866, 1201
BseGI GGATG 3 cut(s) 432, 574, 769
BseJI GATNNNNATC 1 cut(s) 416
BseLI CCNNNNNNNGG 4 cut(s) 223, 554, 639, 1173
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 2 cut(s) 69, 1055
BseNI ACTGG 2 cut(s) 237, 1262
BseRI GAGGAG 4 cut(s) 115, 479, 722, 1091
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 1248
BseYI CCCAGC 1 cut(s) 734
BsgI GTGCAG 1 cut(s) 389
Bsh1236I CGCG 2 cut(s) 249, 329
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 5 cut(s) 24, 216, 386, 719, 1200
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 5 cut(s) 69, 91, 117, 561, 866
BsiWI CGTACG 1 cut(s) 336
BslI CCNNNNNNNGG 4 cut(s) 223, 554, 639, 1173
BsmAI GTCTC 2 cut(s) 704, 879
BsnI GGCC 5 cut(s) 24, 216, 386, 719, 1200
Bso31I GGTCTC 1 cut(s) 879
Bsp1286I GDGCHC 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 1062
Bsp143I GATC 6 cut(s) 6, 43, 417, 681, 874, 1087
BspACI CCGC 6 cut(s) 135, 169, 202, 247, 329, 798
BspANI GGCC 5 cut(s) 24, 216, 386, 719, 1200
BspCNI CTCAG 2 cut(s) 68, 1054
BspFNI CGCG 2 cut(s) 249, 329
BspHI TCATGA 2 cut(s) 240, 703
BspLI GGNNCC 1 cut(s) 559
BspPI GGATC 2 cut(s) 425, 869
BspTI CTTAAG 1 cut(s) 926
BspTNI GGTCTC 1 cut(s) 879
BsrDI GCAATG 1 cut(s) 1065
BsrGI TGTACA 1 cut(s) 1062
BsrI ACTGG 2 cut(s) 237, 1262
BssECI CCNNGG 7 cut(s) 159, 303, 623, 624, 633, 866, 1201
BssMI GATC 6 cut(s) 6, 43, 417, 681, 874, 1087
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 2 cut(s) 159, 633
Bst2UI CCWGG 4 cut(s) 304, 625, 890, 1203
Bst4CI ACNGT 5 cut(s) 36, 106, 448, 649, 715
Bst6I CTCTTC 4 cut(s) 53, 936, 1184, 1356
BstACI GRCGYC 1 cut(s) 156
BstAFI CTTAAG 1 cut(s) 926
BstAUI TGTACA 1 cut(s) 1062
BstC8I GCNNGC 2 cut(s) 327, 549
BstDEI CTNAG 2 cut(s) 55, 1041
BstF5I GGATG 3 cut(s) 432, 574, 769
BstFNI CGCG 2 cut(s) 249, 329
BstKTI GATC 6 cut(s) 9, 46, 420, 684, 877, 1090
BstMAI GTCTC 2 cut(s) 704, 879
BstMBI GATC 6 cut(s) 6, 43, 417, 681, 874, 1087
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 4 cut(s) 383, 557, 731, 839
BstNI CCWGG 4 cut(s) 304, 625, 890, 1203
BstSCI CCNGG 6 cut(s) 68, 302, 623, 865, 888, 1201
BstUI CGCG 2 cut(s) 249, 329
BstV1I GCAGC 1 cut(s) 1248
BstV2I GAAGAC 3 cut(s) 157, 971, 1332
BstX2I RGATCY 1 cut(s) 1087
BstYI RGATCY 1 cut(s) 1087
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 5 cut(s) 24, 216, 386, 719, 1200
BtgZI GCGATG 1 cut(s) 546
BtsCI GGATG 3 cut(s) 432, 574, 769
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 230, 720
Cac8I GCNNGC 2 cut(s) 327, 549
CciI TCATGA 2 cut(s) 240, 703
Cfr13I GGNCC 3 cut(s) 1199, 1271, 1366
CseI GACGC 1 cut(s) 164
CsiI ACCWGGT 1 cut(s) 888
Csp6I GTAC 5 cut(s) 253, 298, 337, 742, 1063
CviAII CATG 6 cut(s) 241, 689, 704, 760, 970, 1232
CviQI GTAC 5 cut(s) 253, 298, 337, 742, 1063
DdeI CTNAG 2 cut(s) 55, 1041
DpnI GATC 6 cut(s) 8, 45, 419, 683, 876, 1089
DpnII GATC 6 cut(s) 6, 43, 417, 681, 874, 1087
DraI TTTAAA 1 cut(s) 850
DraIII CACNNNGTG 2 cut(s) 988, 1012
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 3 cut(s) 22, 214, 717
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 4 cut(s) 53, 936, 1184, 1356
EarI CTCTTC 4 cut(s) 53, 936, 1184, 1356
EciI GGCGGA 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 238
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 2 cut(s) 159, 633
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 879
Eco47I GGWCC 2 cut(s) 1271, 1366
Eco52I CGGCCG 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 960
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 3 cut(s) 1199, 1271, 1366
EcoRI GAATTC 2 cut(s) 27, 1161
EcoRII CCWGG 4 cut(s) 302, 623, 888, 1201
EcoT14I CCWWGG 2 cut(s) 159, 633
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 2 cut(s) 159, 633
FaeI CATG 6 cut(s) 244, 692, 707, 763, 973, 1235
FalI AAGNNNNNCTT 2 cut(s) 1317, 1349
FatI CATG 6 cut(s) 240, 688, 703, 759, 969, 1231
FauI CCCGC 1 cut(s) 791
Fnu4HI GCNGC 1 cut(s) 1262
FokI GGATG 3 cut(s) 419, 581, 776
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 1262
FspBI CTAG 3 cut(s) 816, 1085, 1376
GluI GCNGC 1 cut(s) 1262
GsaI CCCAGC 1 cut(s) 738
GsuI CTGGAG 2 cut(s) 254, 1293
HaeIII GGCC 5 cut(s) 24, 216, 386, 719, 1200
HapII CCGG 5 cut(s) 69, 91, 117, 561, 866
HgaI GACGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 6 cut(s) 244, 692, 707, 763, 973, 1235
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 3 cut(s) 122, 143, 342
HpaII CCGG 5 cut(s) 69, 91, 117, 561, 866
HphI GGTGA 6 cut(s) 94, 238, 331, 412, 532, 1124
Hpy166II GTNNAC 4 cut(s) 255, 300, 523, 610
Hpy188I TCNGA 7 cut(s) 58, 110, 148, 489, 504, 557, 915
Hpy188III TCNNGA 8 cut(s) 241, 271, 401, 704, 878, 1043, 1246, 1310
Hpy8I GTNNAC 4 cut(s) 255, 300, 523, 610
Hpy99I CGWCG 2 cut(s) 143, 158
HpyAV CCTTC 6 cut(s) 109, 274, 310, 466, 491, 1262
HpyCH4III ACNGT 5 cut(s) 36, 106, 448, 649, 715
HpyCH4IV ACGT 5 cut(s) 275, 312, 744, 773, 1156
HpyCH4V TGCA 6 cut(s) 370, 908, 1037, 1058, 1148, 1372
HpyF10VI GCNNNNNNNGC 4 cut(s) 383, 557, 731, 839
HpyF3I CTNAG 2 cut(s) 55, 1041
HpySE526I ACGT 5 cut(s) 275, 312, 744, 773, 1156
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 6 cut(s) 244, 692, 707, 763, 973, 1235
Kzo9I GATC 6 cut(s) 6, 43, 417, 681, 874, 1087
LmnI GCTCC 4 cut(s) 119, 235, 557, 1045
Lsp1109I GCAGC 1 cut(s) 1248
LweI GCATC 6 cut(s) 101, 564, 963, 1024, 1045, 1310
MabI ACCWGGT 1 cut(s) 888
MaeI CTAG 3 cut(s) 816, 1085, 1376
MaeII ACGT 5 cut(s) 275, 312, 744, 773, 1156
MaeIII GTNAC 7 cut(s) 38, 226, 587, 782, 1121, 1276, 1288
MalI GATC 6 cut(s) 8, 45, 419, 683, 876, 1089
MboI GATC 6 cut(s) 6, 43, 417, 681, 874, 1087
MboII GAAGA 8 cut(s) 40, 157, 953, 976, 1163, 1201, 1337, 1373
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 1 cut(s) 1087
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 719
MluCI AATT 6 cut(s) 27, 85, 603, 845, 953, 1161
MluNI TGGCCA 1 cut(s) 719
MlyI GAGTC 2 cut(s) 116, 152
MmeI TCCRAC 3 cut(s) 171, 441, 467
Mox20I TGGCCA 1 cut(s) 719
MroXI GAANNNNTTC 2 cut(s) 98, 290
MscI TGGCCA 1 cut(s) 719
MseI TTAA 3 cut(s) 807, 849, 927
Msp20I TGGCCA 1 cut(s) 719
MspCI CTTAAG 1 cut(s) 926
MspI CCGG 5 cut(s) 69, 91, 117, 561, 866
MspR9I CCNGG 6 cut(s) 70, 304, 625, 867, 890, 1203
MunI CAATTG 1 cut(s) 85
MvaI CCWGG 4 cut(s) 304, 625, 890, 1203
MvnI CGCG 2 cut(s) 249, 329
MwoI GCNNNNNNNGC 4 cut(s) 383, 557, 731, 839
NciI CCSGG 2 cut(s) 70, 867
NdeII GATC 6 cut(s) 6, 43, 417, 681, 874, 1087
NlaIII CATG 6 cut(s) 244, 692, 707, 763, 973, 1235
NlaIV GGNNCC 1 cut(s) 559
NmuCI GTSAC 4 cut(s) 226, 782, 1276, 1288
PagI TCATGA 2 cut(s) 240, 703
PasI CCCWGGG 1 cut(s) 624
PdmI GAANNNNTTC 2 cut(s) 98, 290
PfeI GAWTC 1 cut(s) 342
Pfl23II CGTACG 1 cut(s) 336
PflMI CCANNNNNTGG 1 cut(s) 639
PkrI GCNGC 1 cut(s) 1263
PleI GAGTC 2 cut(s) 116, 151
PpsI GAGTC 2 cut(s) 116, 151
PpuMI RGGWCCY 2 cut(s) 1271, 1366
PsiI TTATAA 1 cut(s) 1221
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 2 cut(s) 1271, 1366
Psp6I CCWGG 4 cut(s) 302, 623, 888, 1201
PspFI CCCAGC 1 cut(s) 734
PspGI CCWGG 4 cut(s) 302, 623, 888, 1201
PspLI CGTACG 1 cut(s) 336
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 3 cut(s) 1199, 1271, 1366
PspPPI RGGWCCY 2 cut(s) 1271, 1366
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PsuI RGATCY 1 cut(s) 1087
RsaI GTAC 5 cut(s) 254, 299, 338, 743, 1064
RsaNI GTAC 5 cut(s) 253, 298, 337, 742, 1063
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 3 cut(s) 807, 849, 927
SatI GCNGC 1 cut(s) 1262
Sau3AI GATC 6 cut(s) 6, 43, 417, 681, 874, 1087
Sau96I GGNCC 3 cut(s) 1199, 1271, 1366
SchI GAGTC 2 cut(s) 116, 152
ScrFI CCNGG 6 cut(s) 70, 304, 625, 867, 890, 1203
SduI GDGCHC 1 cut(s) 240
SexAI ACCWGGT 1 cut(s) 888
SfaNI GCATC 6 cut(s) 101, 564, 963, 1024, 1045, 1310
SinI GGWCC 2 cut(s) 1271, 1366
SmlI CTYRAG 4 cut(s) 583, 926, 1117, 1352
SmoI CTYRAG 4 cut(s) 583, 926, 1117, 1352
Sse9I AATT 6 cut(s) 27, 85, 603, 845, 953, 1161
SsiI CCGC 6 cut(s) 135, 169, 202, 247, 329, 798
SspI AATATT 1 cut(s) 698
SspMI CTAG 3 cut(s) 816, 1085, 1376
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 6 cut(s) 68, 302, 623, 865, 888, 1201
StyI CCWWGG 2 cut(s) 159, 633
TaaI ACNGT 5 cut(s) 36, 106, 448, 649, 715
TaiI ACGT 5 cut(s) 278, 315, 747, 776, 1159
TaqI TCGA 7 cut(s) 141, 153, 184, 294, 680, 1245, 1347
TasI AATT 6 cut(s) 27, 85, 603, 845, 953, 1161
TatI WGTACW 3 cut(s) 252, 297, 1062
TfiI GAWTC 1 cut(s) 342
Tru1I TTAA 3 cut(s) 807, 849, 927
Tru9I TTAA 3 cut(s) 807, 849, 927
TscAI CASTG 2 cut(s) 237, 727
TseFI GTSAC 4 cut(s) 226, 782, 1276, 1288
TseI GCWGC 1 cut(s) 1261
Tsp45I GTSAC 4 cut(s) 226, 782, 1276, 1288
TspDTI ATGAA 3 cut(s) 257, 485, 677
TspGWI ACGGA 2 cut(s) 354, 999
TspRI CASTG 2 cut(s) 237, 727
Van91I CCANNNNNTGG 1 cut(s) 639
Vha464I CTTAAG 1 cut(s) 926
VpaK11BI GGWCC 2 cut(s) 1271, 1366
XapI RAATTY 3 cut(s) 27, 845, 1161
XmnI GAANNNNTTC 2 cut(s) 98, 290
XspI CTAG 3 cut(s) 816, 1085, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.