Prupe.8G105800_v2.0.a1

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009094
Physical Location & Seq
Reverse (-)
13718968 .. 13725136
6169 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G105800.3

Sequence Viewer

Length: 1386 bp
ATGGCGATCACTCTCCACCTCTGCCGCCTTCCATATCGCACCGATCTCTTCTTCTCCGAAAGCCTCGCCGGAAAGGTCTCTCTGAGTCGCCGGAAACTCATCTCCGTCCGATGCTCCGGCGACTCCTCCTCTTCGGCTGCCGTCGAGTCCGATTTCGACGCCAAGGTCTTCCGAAAGAACCTCACTCGAAGCAAGAACTACAATCGCAGAGGCTTCGGCCACAAAGAAGCGACCCTCGAGCTCATGAATCGCGAGTACACCAGTGATATTATAAAGACGTTGAAGGAGAACGGGTTCGAGTACACGTGGGGAAATGTGACGGTGAAGCTCGCCGAGGCGTACGGCTTCTGTTGGGGCGTTGAGAGAGCCGTCCAGATTGCTTACGAGGCCAGAAAGCAGTTTCCTGATGAAAGGATTTGGATTACAAACGAGATCATCCACAATCCAACCGTCAATAAGCGATTGGAAGAGATGAAGGTGGAGAACATCCCAATAGAGGAAGGGGAGAAGCAATTTGAGGTGGTGAACAAGGGTGATGTGGTCATTTTGCCTGCTTTTGGAGCTGGAGTGGATGAGATGCTCAACTTGAGTAACAGAGATGTTCAAATTGTTGACACTACTTGCCCATGGGTTTCCAAGGTTTGGAATACTGTGGAGAAGCACAAGAAAGGAGACTACACATCAATAATTCATGGTAAATATGCTCATGAGGAAACTGTAGCCACTGCTTCTTTTGCTGGGAAGTACGTTATAGTGAAAAACATGGATGAGGCAACATATGTGTGTGACTATATTCTTGGTGGTGAACTGAATGGATCTAGCTCAACTAGAGAGGCGTTTCTAGAGAAATTTAAAAAGGCACTTTCTCCGGGGTTTGATCCGGACAGAGACCTAGTAAAAGTTGGCATTGCGAATCAGACTACGATGCTTAAGGGGGAGACAGAAGAGATTGGAAAATTAGTTGAGAAGACCATGATGCGTAAGCATGGAGTGGAAAATATCAATGACCACTTTGTCAGCTTCAACACAATATGTGATGCGACTCAAGAGCGACAAGATGCAATGTACAAGCTTGTTGAGGAAAAGCTAGATCTTTTGTTAGTCATTGGTGGGTGGAACTCGAGTAACACCTCTCACCTACAAGAGATTGCAGAAGAGCGTGGAATTCCCTCATATTGGGTTGACAGCGAGAAGAGAATAGGCCCTGGAAACAGAATAGCTTATAAACTCAATCATGGCGAGTTGGTTGAGAAAGAGAACTGGCTTCCTCAAGGTCCTATCACTATTGGTGTGACATCTGGTGCGTCTACTCCAGATAAGGTTGTTGAAGACACTCTTATCAGGGTTTTCGATCTCAAGCGTGAAGAAGCCCTTCAACTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
LYTB

Protein Analysis

462

Amino Acids

52.09

Weight (kDa)

5.74

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 272, 1224
AasI GACNNNNNNGTC 2 cut(s) 164, 1013
AccB7I CCANNNNNTGG 1 cut(s) 642
AccI GTMKAC 1 cut(s) 1307
AccII CGCG 1 cut(s) 252
AccIII TCCGGA 1 cut(s) 880
AciI CCGC 1 cut(s) 25
AclWI GGATC 2 cut(s) 823, 872
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 2 cut(s) 848, 1164
AcvI CACGTG 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 5 cut(s) 257, 302, 341, 746, 1067
AfiI CCNNNNNNNGG 4 cut(s) 496, 557, 642, 1176
AflII CTTAAG 1 cut(s) 929
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 5 cut(s) 283, 605, 1024, 1328, 1376
AjnI CCWGG 1 cut(s) 1204
AloI GAACNNNNNNTCC 2 cut(s) 278, 310
AluBI AGCT 8 cut(s) 241, 328, 563, 822, 1020, 1072, 1087, 1220
AluI AGCT 8 cut(s) 241, 328, 563, 822, 1020, 1072, 1087, 1220
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 4 cut(s) 82, 666, 882, 932
AlwI GGATC 2 cut(s) 823, 872
Ama87I CYCGRG 2 cut(s) 236, 1120
Aor13HI TCCGGA 1 cut(s) 880
AoxI GGCC 3 cut(s) 217, 387, 1201
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 2 cut(s) 848, 1164
Asp700I GAANNNNTTC 2 cut(s) 293, 1371
AspS9I GGNCC 2 cut(s) 1202, 1274
AsuC2I CCSGG 1 cut(s) 870
AsuHPI GGTGA 5 cut(s) 334, 535, 545, 815, 1127
AvaI CYCGRG 2 cut(s) 236, 1120
AvaII GGWCC 1 cut(s) 1274
BanII GRGCYC 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 306
BbsI GAAGAC 3 cut(s) 160, 974, 1335
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 124
BceAI ACGGC 3 cut(s) 125, 353, 358
BcgI CGANNNNNNTGC 2 cut(s) 196, 230
BciT130I CCWGG 1 cut(s) 1206
BcnI CCSGG 1 cut(s) 870
BcoDI GTCTC 4 cut(s) 82, 666, 882, 932
BfaI CTAG 6 cut(s) 819, 828, 842, 893, 1088, 1379
BfmI CTRYAG 1 cut(s) 717
BfrI CTTAAG 1 cut(s) 929
BglII AGATCT 1 cut(s) 1090
BisI GCNGC 2 cut(s) 25, 138
BlsI GCNGC 2 cut(s) 26, 139
Bme1390I CCNGG 2 cut(s) 870, 1206
Bme18I GGWCC 1 cut(s) 1274
BmeT110I CYCGRG 2 cut(s) 236, 1120
BmgT120I GGNCC 2 cut(s) 1202, 1274
BmrFI CCNGG 2 cut(s) 870, 1206
BmsI GCATC 6 cut(s) 101, 567, 915, 966, 1027, 1048
BpiI GAAGAC 3 cut(s) 160, 974, 1335
BpmI CTGGAG 2 cut(s) 585, 1296
BpuEI CTTGAG 4 cut(s) 607, 1029, 1254, 1340
BpuMI CCSGG 1 cut(s) 870
BsaAI YACGTR 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 2 cut(s) 82, 882
BsaJI CCNNGG 6 cut(s) 162, 333, 626, 636, 869, 1204
BsaWI WCCGGW 1 cut(s) 880
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 552, 582
Bsc4I CCNNNNNNNGG 4 cut(s) 496, 557, 642, 1176
Bse1I ACTGG 2 cut(s) 261, 1265
Bse3DI GCAATG 2 cut(s) 906, 1068
BseAI TCCGGA 1 cut(s) 880
BseBI CCWGG 1 cut(s) 1206
BseDI CCNNGG 6 cut(s) 162, 333, 626, 636, 869, 1204
BseGI GGATG 4 cut(s) 435, 486, 577, 772
BseLI CCNNNNNNNGG 4 cut(s) 496, 557, 642, 1176
BseMI GCAATG 2 cut(s) 906, 1068
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 2 cut(s) 261, 1265
BseRI GAGGAG 2 cut(s) 115, 118
BseXI GCAGC 1 cut(s) 124
BseYI CCCAGC 1 cut(s) 737
Bsh1236I CGCG 1 cut(s) 252
BshFI GGCC 3 cut(s) 219, 389, 1203
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 2 cut(s) 236, 1120
BsiSI CCGG 5 cut(s) 69, 91, 117, 869, 881
BsiWI CGTACG 1 cut(s) 339
BslI CCNNNNNNNGG 4 cut(s) 496, 557, 642, 1176
BsmAI GTCTC 4 cut(s) 82, 666, 882, 932
BsnI GGCC 3 cut(s) 219, 389, 1203
Bso31I GGTCTC 2 cut(s) 82, 882
BsoBI CYCGRG 2 cut(s) 236, 1120
Bsp1286I GDGCHC 1 cut(s) 243
Bsp13I TCCGGA 1 cut(s) 880
Bsp1407I TGTACA 1 cut(s) 1065
Bsp143I GATC 7 cut(s) 6, 43, 432, 815, 877, 1090, 1351
Bsp19I CCATGG 1 cut(s) 626
Bsp68I TCGCGA 1 cut(s) 252
BspACI CCGC 1 cut(s) 25
BspANI GGCC 3 cut(s) 219, 389, 1203
BspCNI CTCAG 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 880
BspFNI CGCG 1 cut(s) 252
BspHI TCATGA 2 cut(s) 243, 706
BspPI GGATC 2 cut(s) 823, 872
BspQI GCTCTTC 1 cut(s) 1149
BspTI CTTAAG 1 cut(s) 929
BspTNI GGTCTC 2 cut(s) 82, 882
BsrDI GCAATG 2 cut(s) 906, 1068
BsrGI TGTACA 1 cut(s) 1065
BsrI ACTGG 2 cut(s) 261, 1265
BssECI CCNNGG 6 cut(s) 162, 333, 626, 636, 869, 1204
BssMI GATC 7 cut(s) 6, 43, 432, 815, 877, 1090, 1351
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 3 cut(s) 162, 626, 636
Bst2UI CCWGG 1 cut(s) 1206
Bst4CI ACNGT 4 cut(s) 322, 451, 652, 718
Bst6I CTCTTC 6 cut(s) 53, 136, 462, 939, 1149, 1187
BstACI GRCGYC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 929
BstAUI TGTACA 1 cut(s) 1065
BstBAI YACGTR 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 330, 552
BstDEI CTNAG 1 cut(s) 83
BstDSI CCRYGG 1 cut(s) 626
BstF5I GGATG 4 cut(s) 435, 486, 577, 772
BstFNI CGCG 1 cut(s) 252
BstKTI GATC 7 cut(s) 9, 46, 435, 818, 880, 1093, 1354
BstMAI GTCTC 4 cut(s) 82, 666, 882, 932
BstMBI GATC 7 cut(s) 6, 43, 432, 815, 877, 1090, 1351
BstMWI GCNNNNNNNGC 3 cut(s) 386, 560, 734
BstNI CCWGG 1 cut(s) 1206
BstSCI CCNGG 2 cut(s) 868, 1204
BstSFI CTRYAG 1 cut(s) 717
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 124
BstV2I GAAGAC 3 cut(s) 160, 974, 1335
BstX2I RGATCY 2 cut(s) 815, 1090
BstYI RGATCY 2 cut(s) 815, 1090
BsuRI GGCC 3 cut(s) 219, 389, 1203
BtgI CCRYGG 1 cut(s) 626
BtsCI GGATG 4 cut(s) 435, 486, 577, 772
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 2 cut(s) 268, 723
BtuMI TCGCGA 1 cut(s) 252
Cac8I GCNNGC 2 cut(s) 330, 552
CciI TCATGA 2 cut(s) 243, 706
Cfr13I GGNCC 2 cut(s) 1202, 1274
CseI GACGC 2 cut(s) 167, 1293
Csp6I GTAC 5 cut(s) 256, 301, 340, 745, 1066
CviAII CATG 8 cut(s) 244, 627, 692, 707, 763, 973, 986, 1235
CviQI GTAC 5 cut(s) 256, 301, 340, 745, 1066
DdeI CTNAG 1 cut(s) 83
DpnI GATC 7 cut(s) 8, 45, 434, 817, 879, 1092, 1353
DpnII GATC 7 cut(s) 6, 43, 432, 815, 877, 1090, 1351
DraI TTTAAA 1 cut(s) 853
DrdI GACNNNNNNGTC 2 cut(s) 164, 1013
DseDI GACNNNNNNGTC 2 cut(s) 164, 1013
EaeI YGGCCR 1 cut(s) 217
Eam1104I CTCTTC 6 cut(s) 53, 136, 462, 939, 1149, 1187
EarI CTCTTC 6 cut(s) 53, 136, 462, 939, 1149, 1187
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 3 cut(s) 162, 626, 636
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 2 cut(s) 82, 882
Eco47I GGWCC 1 cut(s) 1274
Eco53kI GAGCTC 1 cut(s) 241
Eco72I CACGTG 1 cut(s) 306
Eco88I CYCGRG 2 cut(s) 236, 1120
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 2 cut(s) 1202, 1274
EcoRI GAATTC 1 cut(s) 1164
EcoRII CCWGG 1 cut(s) 1204
EcoT14I CCWWGG 3 cut(s) 162, 626, 636
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 3 cut(s) 162, 626, 636
FaeI CATG 8 cut(s) 247, 630, 695, 710, 766, 976, 989, 1238
FalI AAGNNNNNCTT 3 cut(s) 1320, 1352, 1356
FatI CATG 8 cut(s) 243, 626, 691, 706, 762, 972, 985, 1234
FauNDI CATATG 1 cut(s) 778
FblI GTMKAC 1 cut(s) 1307
Fnu4HI GCNGC 2 cut(s) 25, 138
FokI GGATG 4 cut(s) 422, 473, 584, 779
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 2 cut(s) 25, 138
FspBI CTAG 6 cut(s) 819, 828, 842, 893, 1088, 1379
GluI GCNGC 2 cut(s) 25, 138
GsaI CCCAGC 1 cut(s) 741
GsuI CTGGAG 2 cut(s) 585, 1296
HaeIII GGCC 3 cut(s) 219, 389, 1203
HapII CCGG 5 cut(s) 69, 91, 117, 869, 881
HgaI GACGC 2 cut(s) 167, 1293
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 8 cut(s) 247, 630, 695, 710, 766, 976, 989, 1238
HincII GTYRAC 2 cut(s) 613, 1183
HindII GTYRAC 2 cut(s) 613, 1183
HindIII AAGCTT 1 cut(s) 1070
HinfI GANTC 6 cut(s) 85, 122, 146, 247, 913, 1042
HpaII CCGG 5 cut(s) 69, 91, 117, 869, 881
HphI GGTGA 5 cut(s) 334, 535, 545, 815, 1127
Hpy166II GTNNAC 7 cut(s) 258, 303, 526, 613, 806, 1183, 1308
Hpy188I TCNGA 6 cut(s) 58, 84, 110, 151, 173, 918
Hpy188III TCNNGA 9 cut(s) 244, 251, 373, 404, 707, 842, 881, 1046, 1313
Hpy8I GTNNAC 7 cut(s) 258, 303, 526, 613, 806, 1183, 1308
Hpy99I CGWCG 2 cut(s) 146, 161
HpyAV CCTTC 5 cut(s) 38, 277, 469, 494, 1382
HpyCH4III ACNGT 4 cut(s) 322, 451, 652, 718
HpyCH4IV ACGT 3 cut(s) 278, 305, 747
HpyCH4V TGCA 2 cut(s) 1061, 1151
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 560, 734
HpyF3I CTNAG 1 cut(s) 83
HpySE526I ACGT 3 cut(s) 278, 305, 747
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 8 cut(s) 247, 630, 695, 710, 766, 976, 989, 1238
Kpn2I TCCGGA 1 cut(s) 880
Kzo9I GATC 7 cut(s) 6, 43, 432, 815, 877, 1090, 1351
LguI GCTCTTC 1 cut(s) 1149
LmnI GCTCC 2 cut(s) 119, 560
Lsp1109I GCAGC 1 cut(s) 124
LweI GCATC 6 cut(s) 101, 567, 915, 966, 1027, 1048
MaeI CTAG 6 cut(s) 819, 828, 842, 893, 1088, 1379
MaeII ACGT 3 cut(s) 278, 305, 747
MaeIII GTNAC 5 cut(s) 316, 590, 785, 1124, 1291
MalI GATC 7 cut(s) 8, 45, 434, 817, 879, 1092, 1353
MboI GATC 7 cut(s) 6, 43, 432, 815, 877, 1090, 1351
MflI RGATCY 2 cut(s) 815, 1090
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 6 cut(s) 512, 606, 687, 848, 956, 1164
MlyI GAGTC 4 cut(s) 94, 116, 155, 1036
MmeI TCCRAC 1 cut(s) 470
MroI TCCGGA 1 cut(s) 880
MroXI GAANNNNTTC 2 cut(s) 293, 1371
MseI TTAA 2 cut(s) 852, 930
MslI CAYNNNNRTG 1 cut(s) 781
MspCI CTTAAG 1 cut(s) 929
MspI CCGG 5 cut(s) 69, 91, 117, 869, 881
MspR9I CCNGG 2 cut(s) 870, 1206
MvaI CCWGG 1 cut(s) 1206
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 386, 560, 734
NciI CCSGG 1 cut(s) 870
NcoI CCATGG 1 cut(s) 626
NdeI CATATG 1 cut(s) 778
NdeII GATC 7 cut(s) 6, 43, 432, 815, 877, 1090, 1351
NlaIII CATG 8 cut(s) 247, 630, 695, 710, 766, 976, 989, 1238
NmeAIII GCCGAG 1 cut(s) 358
NmuCI GTSAC 3 cut(s) 316, 785, 1291
NruI TCGCGA 1 cut(s) 252
PaeR7I CTCGAG 2 cut(s) 236, 1120
PagI TCATGA 2 cut(s) 243, 706
PciSI GCTCTTC 1 cut(s) 1149
PdmI GAANNNNTTC 2 cut(s) 293, 1371
PfeI GAWTC 2 cut(s) 247, 913
Pfl23II CGTACG 1 cut(s) 339
PflMI CCANNNNNTGG 1 cut(s) 642
PkrI GCNGC 2 cut(s) 26, 139
PleI GAGTC 4 cut(s) 93, 116, 154, 1036
PmaCI CACGTG 1 cut(s) 306
PmlI CACGTG 1 cut(s) 306
PpsI GAGTC 4 cut(s) 93, 116, 154, 1036
Ppu21I YACGTR 1 cut(s) 306
PpuMI RGGWCCY 1 cut(s) 1274
PsiI TTATAA 2 cut(s) 272, 1224
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 1274
Psp6I CCWGG 1 cut(s) 1204
PspCI CACGTG 1 cut(s) 306
PspFI CCCAGC 1 cut(s) 737
PspGI CCWGG 1 cut(s) 1204
PspLI CGTACG 1 cut(s) 339
PspPI GGNCC 2 cut(s) 1202, 1274
PspPPI RGGWCCY 1 cut(s) 1274
PspXI VCTCGAGB 2 cut(s) 236, 1120
PsuI RGATCY 2 cut(s) 815, 1090
RruI TCGCGA 1 cut(s) 252
RsaI GTAC 5 cut(s) 257, 302, 341, 746, 1067
RsaNI GTAC 5 cut(s) 256, 301, 340, 745, 1066
RseI CAYNNNNRTG 1 cut(s) 781
SacI GAGCTC 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 1149
SaqAI TTAA 2 cut(s) 852, 930
SatI GCNGC 2 cut(s) 25, 138
Sau3AI GATC 7 cut(s) 6, 43, 432, 815, 877, 1090, 1351
Sau96I GGNCC 2 cut(s) 1202, 1274
SchI GAGTC 4 cut(s) 94, 116, 155, 1036
ScrFI CCNGG 2 cut(s) 870, 1206
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 6 cut(s) 101, 567, 915, 966, 1027, 1048
SfcI CTRYAG 1 cut(s) 717
Sfr274I CTCGAG 2 cut(s) 236, 1120
SinI GGWCC 1 cut(s) 1274
SlaI CTCGAG 2 cut(s) 236, 1120
SmiMI CAYNNNNRTG 1 cut(s) 781
SmlI CTYRAG 7 cut(s) 236, 586, 929, 1044, 1120, 1269, 1355
SmoI CTYRAG 7 cut(s) 236, 586, 929, 1044, 1120, 1269, 1355
Sse9I AATT 6 cut(s) 512, 606, 687, 848, 956, 1164
SsiI CCGC 1 cut(s) 25
SspMI CTAG 6 cut(s) 819, 828, 842, 893, 1088, 1379
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 868, 1204
StyI CCWWGG 3 cut(s) 162, 626, 636
TaaI ACNGT 4 cut(s) 322, 451, 652, 718
TaiI ACGT 3 cut(s) 281, 308, 750
TaqI TCGA 7 cut(s) 144, 156, 187, 237, 297, 1121, 1350
TasI AATT 6 cut(s) 512, 606, 687, 848, 956, 1164
TatI WGTACW 3 cut(s) 255, 300, 1065
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 2 cut(s) 247, 913
Tru1I TTAA 2 cut(s) 852, 930
Tru9I TTAA 2 cut(s) 852, 930
TscAI CASTG 2 cut(s) 268, 730
TseFI GTSAC 3 cut(s) 316, 785, 1291
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 3 cut(s) 316, 785, 1291
TspDTI ATGAA 4 cut(s) 260, 423, 488, 680
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 2 cut(s) 268, 730
Van91I CCANNNNNTGG 1 cut(s) 642
Vha464I CTTAAG 1 cut(s) 929
VpaK11BI GGWCC 1 cut(s) 1274
XapI RAATTY 2 cut(s) 848, 1164
XbaI TCTAGA 1 cut(s) 841
XhoI CTCGAG 2 cut(s) 236, 1120
XmiI GTMKAC 1 cut(s) 1307
XmnI GAANNNNTTC 2 cut(s) 293, 1371
XspI CTAG 6 cut(s) 819, 828, 842, 893, 1088, 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.