MD05G1077000.v1.1
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0022213
Physical Location & Seq
Reverse (-)
16475424 .. 16476150
727 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1077000.v1.1.491

Sequence Viewer

Length: 327 bp
ATGGCTGGGGGCGGAATAGGTCCGACGAAAGGTGGAAAAGAATATCCGGGCAAACTCACTGCTAGGGTCCTTCTCACATGTATGGTGGCAGCTACCGGTGGTTTGATCTTTGGCTACGATCTCGGAGTTTCAGGTGGTGTGACATCAATGGATTCTTTCCTGAAAAAGTTTTTTCCAGAGGTTTACATTAAGGAATCTACGATCAAACCTTCCGATGATCAGTACTGCAAGTTCGACAGCCAGAAGTTGACGCTCTTCACATCGTCATTATATTTAGCTGCTCGTGTCGTGTCTTTGTGCATCAACTGTAACCAGAAAGTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

109

Amino Acids

11.63

Weight (kDa)

8.93

Isoelectric Point (pI)

25.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 27 - 98 4.8e-07 Sugar (and other) transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022213)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g15486
malus_domestica MD05G1077000.v1.1 MD16G1190500.v1.1
pyrus_communis pycom16g15970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 12
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 29
AflIII ACRYGT 1 cut(s) 77
AgeI ACCGGT 1 cut(s) 95
AluBI AGCT 2 cut(s) 92, 278
AluI AGCT 2 cut(s) 92, 278
ApeKI GCWGC 2 cut(s) 89, 278
AsiGI ACCGGT 1 cut(s) 95
AspS9I GGNCC 2 cut(s) 20, 67
AsuC2I CCSGG 1 cut(s) 48
AvaII GGWCC 2 cut(s) 20, 67
BauI CACGAG 1 cut(s) 282
BbvI GCAGC 2 cut(s) 101, 265
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 1 cut(s) 48
BfaI CTAG 1 cut(s) 63
BisI GCNGC 2 cut(s) 90, 279
BlsI GCNGC 2 cut(s) 91, 280
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 1 cut(s) 48
Bme18I GGWCC 2 cut(s) 20, 67
BmgT120I GGNCC 2 cut(s) 20, 67
BmiI GGNNCC 1 cut(s) 68
BmrFI CCNGG 1 cut(s) 48
BmsI GCATC 1 cut(s) 309
BpuMI CCSGG 1 cut(s) 48
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 29
BseXI GCAGC 2 cut(s) 101, 265
BseYI CCCAGC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 95
BsiSI CCGG 2 cut(s) 47, 96
BslI CCNNNNNNNGG 1 cut(s) 29
Bsp143I GATC 4 cut(s) 105, 118, 201, 217
BspACI CCGC 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 68
BspQI GCTCTTC 1 cut(s) 260
BsrFI RCCGGY 1 cut(s) 95
BssAI RCCGGY 1 cut(s) 95
BssMI GATC 4 cut(s) 105, 118, 201, 217
BssSI CACGAG 1 cut(s) 282
Bst2BI CACGAG 1 cut(s) 282
Bst4CI ACNGT 1 cut(s) 308
Bst6I CTCTTC 1 cut(s) 260
BstKTI GATC 4 cut(s) 108, 121, 204, 220
BstMBI GATC 4 cut(s) 105, 118, 201, 217
BstNSI RCATGY 1 cut(s) 81
BstSCI CCNGG 1 cut(s) 46
BstV1I GCAGC 2 cut(s) 101, 265
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 57
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 2 cut(s) 20, 67
CseI GACGC 1 cut(s) 259
Csp6I GTAC 1 cut(s) 223
CspAI ACCGGT 1 cut(s) 95
CviAII CATG 1 cut(s) 78
CviJI RGCY 5 cut(s) 5, 92, 114, 240, 278
CviKI_1 RGCY 5 cut(s) 5, 92, 114, 240, 278
CviQI GTAC 1 cut(s) 223
DpnI GATC 4 cut(s) 107, 120, 203, 219
DpnII GATC 4 cut(s) 105, 118, 201, 217
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 20, 67
EcoO109I RGGNCCY 1 cut(s) 67
FaeI CATG 1 cut(s) 81
FaiI YATR 3 cut(s) 79, 83, 271
FatI CATG 1 cut(s) 77
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 2 cut(s) 90, 279
Fsp4HI GCNGC 2 cut(s) 90, 279
FspBI CTAG 1 cut(s) 63
GluI GCNGC 2 cut(s) 90, 279
GsaI CCCAGC 1 cut(s) 9
HapII CCGG 2 cut(s) 47, 96
HgaI GACGC 1 cut(s) 259
Hin1II CATG 1 cut(s) 81
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HinfI GANTC 2 cut(s) 152, 194
HpaII CCGG 2 cut(s) 47, 96
Hpy166II GTNNAC 2 cut(s) 184, 249
Hpy188I TCNGA 3 cut(s) 24, 125, 214
Hpy188III TCNNGA 2 cut(s) 160, 176
Hpy8I GTNNAC 2 cut(s) 184, 249
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 2 cut(s) 80, 219
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4V TGCA 2 cut(s) 228, 300
Hsp92II CATG 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 4 cut(s) 105, 118, 201, 217
LguI GCTCTTC 1 cut(s) 260
LpnPI CCDG 6 cut(s) 60, 109, 117, 173, 189, 254
Lsp1109I GCAGC 2 cut(s) 101, 265
LweI GCATC 1 cut(s) 309
MaeI CTAG 1 cut(s) 63
MaeIII GTNAC 2 cut(s) 139, 308
MalI GATC 4 cut(s) 107, 120, 203, 219
MboI GATC 4 cut(s) 105, 118, 201, 217
MboII GAAGA 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 1 cut(s) 172
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 80
MspI CCGG 2 cut(s) 47, 96
MspR9I CCNGG 1 cut(s) 48
NciI CCSGG 1 cut(s) 48
NdeII GATC 4 cut(s) 105, 118, 201, 217
NlaIII CATG 1 cut(s) 81
NlaIV GGNNCC 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 139
NspI RCATGY 1 cut(s) 81
PciI ACATGT 1 cut(s) 77
PciSI GCTCTTC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 152, 194
PinAI ACCGGT 1 cut(s) 95
PkrI GCNGC 2 cut(s) 91, 280
PpuMI RGGWCCY 1 cut(s) 67
PscI ACATGT 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 68
PspPI GGNCC 2 cut(s) 20, 67
PspPPI RGGWCCY 1 cut(s) 67
PsrI GAACNNNNNNTAC 2 cut(s) 215, 247
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 2 cut(s) 90, 279
Sau3AI GATC 4 cut(s) 105, 118, 201, 217
Sau96I GGNCC 2 cut(s) 20, 67
ScaI AGTACT 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 48
SetI ASST 7 cut(s) 22, 34, 94, 136, 183, 211, 280
SfaNI GCATC 1 cut(s) 309
SinI GGWCC 2 cut(s) 20, 67
SmiMI CAYNNNNRTG 1 cut(s) 80
SsiI CCGC 1 cut(s) 12
SspMI CTAG 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 308
TaqI TCGA 1 cut(s) 234
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 2 cut(s) 152, 194
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 2 cut(s) 89, 278
Tsp45I GTSAC 1 cut(s) 139
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 2 cut(s) 20, 67
XceI RCATGY 1 cut(s) 81
XspI CTAG 1 cut(s) 63
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.