MD05G1077700.v1.1

Presequence protease 1, chloroplastic mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024451
Physical Location & Seq
Forward (+)
16572543 .. 16574772
2230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1077700.v1.1.491

Sequence Viewer

Length: 855 bp
ATGTTTTTGGGTGGCTTCGTGAACAAATATTTGGCGTGGCTTCGTGAACAAATATTTGGCGTCAGTCAAGTGGAGTTTCTGCAAGCGCTTGAAAAGAAGGTTTATCAAGATTGGGACGGCATTTCTTCATCTCTAGAGGAAATTCGCAAGTCAGTTAATCATGTTGGAAAATCTGTTATCCTTCATGACAGTGGTTATCGGATTAATGGAAGTACACTTGTTATATCCAAATTTATTAATGATACATGGTTGCATGATCGGGTAGGAATTAGTGGAGAAGCATCTGCTTCTTGTTACTTTGATACTAACACAGGTACAAATCTGCTCAGTTATAAATTTGCATGGACTTTGAATTTCTGTTTGAGAACCTCACTGTCACTGACGATTTGTTTTGTAGGCGTATTCTCCTTCTTGTCTGATCGTGACCCTAACTTGTTAAAGACGCTTGATGTATACGATGGAACTGGAGATTTTCTACGGAAATTAGAAATGGATGATGATACTCTCAGAAAGGCCATTGTGCTGGCGCTCAGAGATGATTCATACCAACTTCTTGATGACAAAGTTATAGCGGGTGTTTTTTTGAAGCGGGATTTAACGGGAGTCACAGAAGAAGAAAGGCAGAGGAGGCATAAAGAGATATTGTCAACCAGCTTGAAGGACTTCAAGAAATTTGCAGATGCATCCGATGCAGTTAAAGGCAAAGGCGTTGTGGTCACAGTGGGAGCGCCATTGGGTGTCCAAGCAGCACTAAAGAAGCGACCTAAACTCTTCAAACTAAAGATAGTGCCCCTCTTCAGCGAGCTCGACATCGTATCTGGAGGACTACTACTTCAGAAAATGCTGCAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

285

Amino Acids

31.89

Weight (kDa)

8.6

Isoelectric Point (pI)

25.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PreP_C PF22516 51 - 111 3.8e-10 PreP C-terminal domain
PreP_C PF22516 132 - 241 7.3e-30 PreP C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024451)

Species Orthologous Gene IDs
malus_domestica MD05G1077700.v1.1 MD05G1078700.v1.1
pyrus_communis pycom05g07150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AccI GTMKAC 1 cut(s) 453
AciI CCGC 2 cut(s) 572, 589
AcsI RAATTY 5 cut(s) 141, 230, 335, 352, 671
AcuI CTGAAG 2 cut(s) 781, 818
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 2 cut(s) 214, 316
AfeI AGCGCT 1 cut(s) 87
AgsI TTSAA 6 cut(s) 92, 352, 586, 658, 667, 775
AjuI GAANNNNNNNTTGG 4 cut(s) 14, 39, 46, 71
AluBI AGCT 2 cut(s) 654, 805
AluI AGCT 2 cut(s) 654, 805
Alw21I GWGCWC 1 cut(s) 807
Aor51HI AGCGCT 1 cut(s) 87
AoxI GGCC 1 cut(s) 513
ApeKI GCWGC 2 cut(s) 746, 844
ApoI RAATTY 5 cut(s) 141, 230, 335, 352, 671
AseI ATTAAT 2 cut(s) 204, 237
Asp700I GAANNNNTTC 1 cut(s) 662
AspLEI GCGC 3 cut(s) 88, 529, 730
BaeGI GKGCMC 1 cut(s) 792
BanII GRGCYC 1 cut(s) 807
Bbv12I GWGCWC 1 cut(s) 807
BbvI GCAGC 2 cut(s) 758, 831
BccI CCATC 1 cut(s) 452
BceAI ACGGC 1 cut(s) 133
BfaI CTAG 1 cut(s) 134
BfoI RGCGCY 3 cut(s) 89, 530, 731
BisI GCNGC 2 cut(s) 747, 845
BlsI GCNGC 2 cut(s) 748, 846
BmsI GCATC 4 cut(s) 290, 670, 679, 692
BpmI CTGGAG 2 cut(s) 486, 840
BsaHI GRCGYC 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 469
BseGI GGATG 2 cut(s) 499, 683
BseMII CTCAG 3 cut(s) 340, 520, 544
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 1 cut(s) 640
BseSI GKGCMC 1 cut(s) 792
BseXI GCAGC 2 cut(s) 758, 831
BshFI GGCC 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 807
BslFI GGGAC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 515
Bsp1286I GDGCHC 2 cut(s) 792, 807
Bsp143I GATC 2 cut(s) 256, 418
BspACI CCGC 2 cut(s) 572, 589
BspANI GGCC 1 cut(s) 515
BspCNI CTCAG 3 cut(s) 339, 519, 543
BspHI TCATGA 1 cut(s) 184
BsrI ACTGG 1 cut(s) 469
BssMI GATC 2 cut(s) 256, 418
BssNAI GTATAC 1 cut(s) 454
BssNI GRCGYC 1 cut(s) 60
Bst1107I GTATAC 1 cut(s) 454
Bst4CI ACNGT 3 cut(s) 191, 375, 721
Bst6I CTCTTC 2 cut(s) 776, 800
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 689
BstC8I GCNNGC 3 cut(s) 84, 525, 803
BstDEI CTNAG 3 cut(s) 326, 506, 530
BstF5I GGATG 2 cut(s) 499, 683
BstH2I RGCGCY 3 cut(s) 89, 530, 731
BstHHI GCGC 3 cut(s) 88, 529, 730
BstKTI GATC 2 cut(s) 259, 421
BstMBI GATC 2 cut(s) 256, 418
BstMWI GCNNNNNNNGC 2 cut(s) 628, 689
BstSLI GKGCMC 1 cut(s) 792
BstV1I GCAGC 2 cut(s) 758, 831
BstXI CCANNNNNNTGG 1 cut(s) 523
BstZ17I GTATAC 1 cut(s) 454
BsuRI GGCC 1 cut(s) 515
BtsCI GGATG 2 cut(s) 499, 683
BtsIMutI CAGTG 4 cut(s) 196, 371, 377, 726
Cac8I GCNNGC 3 cut(s) 84, 525, 803
CciI TCATGA 1 cut(s) 184
CfoI GCGC 3 cut(s) 88, 529, 730
CseI GACGC 2 cut(s) 49, 451
Csp6I GTAC 2 cut(s) 213, 315
CviAII CATG 5 cut(s) 161, 185, 246, 254, 342
CviJI RGCY 5 cut(s) 15, 40, 515, 654, 805
CviKI_1 RGCY 5 cut(s) 15, 40, 515, 654, 805
CviQI GTAC 2 cut(s) 213, 315
DdeI CTNAG 3 cut(s) 326, 506, 530
DpnI GATC 2 cut(s) 258, 420
DpnII GATC 2 cut(s) 256, 418
Eam1104I CTCTTC 2 cut(s) 776, 800
EarI CTCTTC 2 cut(s) 776, 800
Ecl136II GAGCTC 1 cut(s) 805
Eco24I GRGCYC 1 cut(s) 807
Eco47III AGCGCT 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 805
Eco57I CTGAAG 2 cut(s) 781, 818
EcoICRI GAGCTC 1 cut(s) 805
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 807
FaeI CATG 5 cut(s) 164, 188, 249, 257, 345
FaqI GGGAC 1 cut(s) 128
FatI CATG 5 cut(s) 160, 184, 245, 253, 341
FauI CCCGC 2 cut(s) 565, 582
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 2 cut(s) 747, 845
FokI GGATG 2 cut(s) 506, 670
FriOI GRGCYC 1 cut(s) 807
Fsp4HI GCNGC 2 cut(s) 747, 845
FspBI CTAG 1 cut(s) 134
GlaI GCGC 3 cut(s) 87, 528, 729
GluI GCNGC 2 cut(s) 747, 845
GsuI CTGGAG 2 cut(s) 486, 840
HaeII RGCGCY 3 cut(s) 89, 530, 731
HaeIII GGCC 1 cut(s) 515
HgaI GACGC 2 cut(s) 49, 451
HhaI GCGC 3 cut(s) 88, 529, 730
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 5 cut(s) 164, 188, 249, 257, 345
Hin6I GCGC 3 cut(s) 86, 527, 728
HinP1I GCGC 3 cut(s) 86, 527, 728
HincII GTYRAC 1 cut(s) 648
HindII GTYRAC 1 cut(s) 648
HinfI GANTC 2 cut(s) 539, 603
Hpy166II GTNNAC 5 cut(s) 22, 47, 215, 454, 648
Hpy188I TCNGA 6 cut(s) 201, 418, 509, 533, 688, 837
Hpy188III TCNNGA 9 cut(s) 19, 44, 107, 134, 185, 422, 554, 667, 819
Hpy8I GTNNAC 5 cut(s) 22, 47, 215, 454, 648
HpyAV CCTTC 4 cut(s) 91, 191, 418, 652
HpyCH4III ACNGT 3 cut(s) 191, 375, 721
HpyCH4V TGCA 7 cut(s) 82, 253, 341, 677, 683, 692, 847
HpyF10VI GCNNNNNNNGC 2 cut(s) 628, 689
HpyF3I CTNAG 3 cut(s) 326, 506, 530
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 5 cut(s) 164, 188, 249, 257, 345
HspAI GCGC 3 cut(s) 86, 527, 728
Kzo9I GATC 2 cut(s) 256, 418
LmnI GCTCC 1 cut(s) 725
LpnPI CCDG 5 cut(s) 297, 450, 509, 664, 804
Lsp1109I GCAGC 2 cut(s) 758, 831
LweI GCATC 4 cut(s) 290, 670, 679, 692
MaeI CTAG 1 cut(s) 134
MaeIII GTNAC 5 cut(s) 293, 375, 422, 604, 715
MalI GATC 2 cut(s) 258, 420
MboI GATC 2 cut(s) 256, 418
MboII GAAGA 5 cut(s) 117, 623, 626, 763, 787
MhlI GDGCHC 2 cut(s) 792, 807
MluCI AATT 8 cut(s) 141, 230, 267, 335, 352, 482, 671, 850
MlyI GAGTC 1 cut(s) 612
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 6 cut(s) 130, 379, 618, 621, 803, 815
Mph1103I ATGCAT 1 cut(s) 685
MroXI GAANNNNTTC 1 cut(s) 662
MseI TTAA 7 cut(s) 156, 204, 237, 437, 596, 696, 853
MslI CAYNNNNRTG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 628, 689
NdeII GATC 2 cut(s) 256, 418
NlaIII CATG 5 cut(s) 164, 188, 249, 257, 345
NmuCI GTSAC 4 cut(s) 375, 422, 604, 715
NsiI ATGCAT 1 cut(s) 685
PagI TCATGA 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 662
PfeI GAWTC 1 cut(s) 539
PkrI GCNGC 2 cut(s) 748, 846
PleI GAGTC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 611
PshBI ATTAAT 2 cut(s) 204, 237
PsiI TTATAA 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 807
RsaI GTAC 2 cut(s) 214, 316
RsaNI GTAC 2 cut(s) 213, 315
RseI CAYNNNNRTG 1 cut(s) 189
SacI GAGCTC 1 cut(s) 807
SaqAI TTAA 7 cut(s) 156, 204, 237, 437, 596, 696, 853
SatI GCNGC 2 cut(s) 747, 845
Sau3AI GATC 2 cut(s) 256, 418
SchI GAGTC 1 cut(s) 612
SduI GDGCHC 2 cut(s) 792, 807
SetI ASST 6 cut(s) 102, 316, 371, 656, 766, 807
SfaNI GCATC 4 cut(s) 290, 670, 679, 692
SmiMI CAYNNNNRTG 1 cut(s) 189
Sse9I AATT 8 cut(s) 141, 230, 267, 335, 352, 482, 671, 850
SsiI CCGC 2 cut(s) 572, 589
SspI AATATT 2 cut(s) 29, 54
SspMI CTAG 1 cut(s) 134
SstI GAGCTC 1 cut(s) 807
TaaI ACNGT 3 cut(s) 191, 375, 721
TaqI TCGA 1 cut(s) 807
TasI AATT 8 cut(s) 141, 230, 267, 335, 352, 482, 671, 850
TatI WGTACW 1 cut(s) 212
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 7 cut(s) 156, 204, 237, 437, 596, 696, 853
Tru9I TTAA 7 cut(s) 156, 204, 237, 437, 596, 696, 853
TscAI CASTG 4 cut(s) 196, 378, 384, 726
TseFI GTSAC 4 cut(s) 375, 422, 604, 715
TseI GCWGC 2 cut(s) 746, 844
Tsp45I GTSAC 4 cut(s) 375, 422, 604, 715
TspDTI ATGAA 3 cut(s) 117, 173, 531
TspGWI ACGGA 1 cut(s) 493
TspRI CASTG 4 cut(s) 196, 378, 384, 726
VspI ATTAAT 2 cut(s) 204, 237
XapI RAATTY 5 cut(s) 141, 230, 335, 352, 671
XbaI TCTAGA 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 662
XspI CTAG 1 cut(s) 134
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.