pycom05g07150

Presequence protease 1, chloroplastic mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0024451
Physical Location & Seq
Forward (+)
10125724 .. 10128511
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07150.4

Sequence Viewer

Length: 1056 bp
ATGGCAGGACGTGCTGAAGACCTATTTGAACTGATCCACTACATTCTTCAAGAAGTCCGATTTACTGAGCCAGAACCGCTTAAGCATAAGGTGTCCCAGTTTATAGCTGAGTACCAAGACAATGCCAGTCGTCATGAAAATGCTGCAGCAAGAATGGATGCAAAATTAAATGTTTCTGGGTGGCTTCGTGAACAAATATTTGGCGTCAGTCAAGTGGAGTTTCTGCAAGCGCTTGAAAAGAAGGTTGATCAAGATTGGGATGGCATTTCTTCATCTCTAGAGGAAATTTGCAAGTCAGTAATTTGTAAGAAAGGTTGCTTCATAATTATGACTGCTGAAGGGAAGAATCCTACTCAATCTCAGAAATTTGTGAGCAAATTTCTGGATTTGCTGCCTAGCAAGTCTCCCTTTGCAAGAAGAACTACTTGGAAAGCTACACTACCTTCTGAAAATGAGGCCATCATAATACCTACGAAGGTTAATCATGTTGGAAAATCTGTTATCCTTCATGACAGTGGTTATCGGATTAATGGAAGTACACTTATATCCAAATTTATTAATGATACATGGTTGCATGATCTGGTAGGAATTAGTGGAGAAGCATCTGCTTCCTGTTACTTTGATATTAACACAGGCGTATTCTCCTTCTTGTATGTTCGTGACCCTAACTTGTTAAAGACGCTTGATGTATACGATGGAACTGAAGATTTTCTACGGAAATTAGAAATGGATGATGATACTCTCAGAAAGGCCATTGTGCTGGCGCTCAGAGATGATTCATACCAACTTCTTGATGACAAAGGTTATAGCGGTTCTTTGAAGGGGGATTTAATGGGAGTCACAGAAGAAGAAAGGCAGAGGAGGCATAAAGGGATATTGTCAACCAGCTTGAAGGACTTCAAGAAATTTGCAGATGCATTCGATGCAGTTAAAGGCAAAGGCGTTGTGGTCACAGTGGGAGCGCCATTGGGTGTCAAAGCAGCACTAAAGAAGAGACCTAAACTCTTCAAATTAAAGATAGTGCCCCTCTTCAGCGAGCTCGACATCGTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

352

Amino Acids

39.4

Weight (kDa)

7.06

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024451)

Species Orthologous Gene IDs
malus_domestica MD05G1077700.v1.1 MD05G1078700.v1.1
pyrus_communis pycom05g07150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 690
AciI CCGC 2 cut(s) 77, 810
AclWI GGATC 1 cut(s) 28
AcsI RAATTY 5 cut(s) 285, 365, 377, 551, 905
AcuI CTGAAG 4 cut(s) 36, 357, 723, 1015
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 113, 538
AfeI AGCGCT 1 cut(s) 231
AflII CTTAAG 1 cut(s) 80
AgsI TTSAA 7 cut(s) 29, 50, 236, 820, 892, 901, 1009
AjiI CACGTC 1 cut(s) 11
AjuI GAANNNNNNNTTGG 4 cut(s) 183, 215, 409, 441
AluBI AGCT 4 cut(s) 107, 434, 888, 1039
AluI AGCT 4 cut(s) 107, 434, 888, 1039
Alw21I GWGCWC 1 cut(s) 1041
Alw26I GTCTC 2 cut(s) 408, 988
AlwI GGATC 1 cut(s) 28
Aor51HI AGCGCT 1 cut(s) 231
AoxI GGCC 2 cut(s) 456, 750
ApeKI GCWGC 4 cut(s) 143, 146, 391, 980
ApoI RAATTY 5 cut(s) 285, 365, 377, 551, 905
AseI ATTAAT 2 cut(s) 528, 558
Asp700I GAANNNNTTC 2 cut(s) 708, 896
AspLEI GCGC 3 cut(s) 232, 766, 964
BaeGI GKGCMC 1 cut(s) 1026
BanII GRGCYC 1 cut(s) 1041
BarI GAAGNNNNNNTAC 2 cut(s) 696, 728
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 1041
BbvI GCAGC 4 cut(s) 130, 158, 378, 992
BccI CCATC 3 cut(s) 254, 467, 689
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 2 cut(s) 408, 988
BfaI CTAG 2 cut(s) 278, 396
BfmI CTRYAG 1 cut(s) 144
BfoI RGCGCY 3 cut(s) 233, 767, 965
BfrI CTTAAG 1 cut(s) 80
BisI GCNGC 4 cut(s) 144, 147, 392, 981
BlsI GCNGC 4 cut(s) 145, 148, 393, 982
BmgBI CACGTC 1 cut(s) 11
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 4 cut(s) 148, 611, 904, 913
BmuI ACTGGG 1 cut(s) 91
BpiI GAAGAC 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 988
Bse1I ACTGG 2 cut(s) 97, 126
BseGI GGATG 3 cut(s) 163, 265, 736
BseMII CTCAG 5 cut(s) 57, 99, 374, 757, 781
BseNI ACTGG 2 cut(s) 97, 126
BseRI GAGGAG 1 cut(s) 874
BseSI GKGCMC 1 cut(s) 1026
BseXI GCAGC 4 cut(s) 130, 158, 378, 992
BshFI GGCC 2 cut(s) 458, 752
BsiHKAI GWGCWC 1 cut(s) 1041
BslFI GGGAC 1 cut(s) 79
BsmAI GTCTC 2 cut(s) 408, 988
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 917
BsnI GGCC 2 cut(s) 458, 752
Bso31I GGTCTC 1 cut(s) 988
Bsp1286I GDGCHC 2 cut(s) 1026, 1041
Bsp143I GATC 3 cut(s) 33, 247, 577
BspACI CCGC 2 cut(s) 77, 810
BspANI GGCC 2 cut(s) 458, 752
BspCNI CTCAG 5 cut(s) 58, 100, 373, 756, 780
BspHI TCATGA 2 cut(s) 133, 508
BspMAI CTGCAG 1 cut(s) 148
BspPI GGATC 1 cut(s) 28
BspTI CTTAAG 1 cut(s) 80
BspTNI GGTCTC 1 cut(s) 988
BsrI ACTGG 2 cut(s) 97, 126
BssMI GATC 3 cut(s) 33, 247, 577
BssNAI GTATAC 1 cut(s) 691
BssNI GRCGYC 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 691
Bst4CI ACNGT 2 cut(s) 515, 955
Bst6I CTCTTC 3 cut(s) 986, 1010, 1034
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 80
BstAPI GCANNNNNTGC 2 cut(s) 11, 923
BstC8I GCNNGC 3 cut(s) 228, 762, 1037
BstDEI CTNAG 5 cut(s) 66, 108, 360, 743, 767
BstF5I GGATG 3 cut(s) 163, 265, 736
BstH2I RGCGCY 3 cut(s) 233, 767, 965
BstHHI GCGC 3 cut(s) 232, 766, 964
BstKTI GATC 3 cut(s) 36, 250, 580
BstMAI GTCTC 2 cut(s) 408, 988
BstMBI GATC 3 cut(s) 33, 247, 577
BstMWI GCNNNNNNNGC 4 cut(s) 11, 76, 862, 923
BstSFI CTRYAG 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 1026
BstV1I GCAGC 4 cut(s) 130, 158, 378, 992
BstV2I GAAGAC 1 cut(s) 24
BstXI CCANNNNNNTGG 1 cut(s) 760
BstZ17I GTATAC 1 cut(s) 691
BsuRI GGCC 2 cut(s) 458, 752
BtrI CACGTC 1 cut(s) 11
BtsCI GGATG 3 cut(s) 163, 265, 736
BtsIMutI CAGTG 2 cut(s) 520, 960
Cac8I GCNNGC 3 cut(s) 228, 762, 1037
CciI TCATGA 2 cut(s) 133, 508
CfoI GCGC 3 cut(s) 232, 766, 964
CseI GACGC 2 cut(s) 193, 688
Csp6I GTAC 2 cut(s) 112, 537
CviAII CATG 5 cut(s) 134, 485, 509, 567, 575
CviJI RGCY 8 cut(s) 70, 107, 184, 434, 458, 752, 888, 1039
CviKI_1 RGCY 8 cut(s) 70, 107, 184, 434, 458, 752, 888, 1039
CviQI GTAC 2 cut(s) 112, 537
DdeI CTNAG 5 cut(s) 66, 108, 360, 743, 767
DpnI GATC 3 cut(s) 35, 249, 579
DpnII GATC 3 cut(s) 33, 247, 577
Eam1104I CTCTTC 3 cut(s) 986, 1010, 1034
EarI CTCTTC 3 cut(s) 986, 1010, 1034
Ecl136II GAGCTC 1 cut(s) 1039
Eco24I GRGCYC 1 cut(s) 1041
Eco31I GGTCTC 1 cut(s) 988
Eco47III AGCGCT 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 1039
Eco57I CTGAAG 4 cut(s) 36, 357, 723, 1015
EcoICRI GAGCTC 1 cut(s) 1039
EcoT22I ATGCAT 1 cut(s) 919
EcoT38I GRGCYC 1 cut(s) 1041
FaeI CATG 5 cut(s) 137, 488, 512, 570, 578
FalI AAGNNNNNCTT 4 cut(s) 392, 424, 409, 441
FaqI GGGAC 1 cut(s) 79
FatI CATG 5 cut(s) 133, 484, 508, 566, 574
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 4 cut(s) 144, 147, 392, 981
FokI GGATG 3 cut(s) 170, 272, 743
FriOI GRGCYC 1 cut(s) 1041
Fsp4HI GCNGC 4 cut(s) 144, 147, 392, 981
FspBI CTAG 2 cut(s) 278, 396
GlaI GCGC 3 cut(s) 231, 765, 963
GluI GCNGC 4 cut(s) 144, 147, 392, 981
HaeII RGCGCY 3 cut(s) 233, 767, 965
HaeIII GGCC 2 cut(s) 458, 752
HgaI GACGC 2 cut(s) 193, 688
HhaI GCGC 3 cut(s) 232, 766, 964
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 5 cut(s) 137, 488, 512, 570, 578
Hin6I GCGC 3 cut(s) 230, 764, 962
HinP1I GCGC 3 cut(s) 230, 764, 962
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HinfI GANTC 3 cut(s) 346, 776, 837
Hpy166II GTNNAC 4 cut(s) 191, 539, 691, 882
Hpy188I TCNGA 6 cut(s) 59, 363, 448, 525, 746, 770
Hpy8I GTNNAC 4 cut(s) 191, 539, 691, 882
HpyAV CCTTC 8 cut(s) 235, 332, 453, 469, 515, 655, 814, 886
HpyCH4III ACNGT 2 cut(s) 515, 955
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 9 cut(s) 146, 161, 226, 291, 413, 574, 911, 917, 926
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 76, 862, 923
HpyF3I CTNAG 5 cut(s) 66, 108, 360, 743, 767
HpySE526I ACGT 1 cut(s) 10
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 5 cut(s) 137, 488, 512, 570, 578
HspAI GCGC 3 cut(s) 230, 764, 962
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 3 cut(s) 33, 247, 577
LmnI GCTCC 1 cut(s) 959
Lsp1109I GCAGC 4 cut(s) 130, 158, 378, 992
LweI GCATC 4 cut(s) 148, 611, 904, 913
MaeI CTAG 2 cut(s) 278, 396
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 4 cut(s) 614, 659, 838, 949
MalI GATC 3 cut(s) 35, 249, 579
MboI GATC 3 cut(s) 33, 247, 577
MhlI GDGCHC 2 cut(s) 1026, 1041
MlyI GAGTC 1 cut(s) 846
MmeI TCCRAC 1 cut(s) 469
MnlI CCTC 5 cut(s) 274, 448, 852, 855, 1037
Mph1103I ATGCAT 1 cut(s) 919
MroXI GAANNNNTTC 2 cut(s) 708, 896
MslI CAYNNNNRTG 3 cut(s) 138, 326, 513
MspCI CTTAAG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 917
MwoI GCNNNNNNNGC 4 cut(s) 11, 76, 862, 923
NdeII GATC 3 cut(s) 33, 247, 577
NlaIII CATG 5 cut(s) 137, 488, 512, 570, 578
NmuCI GTSAC 3 cut(s) 659, 838, 949
NsiI ATGCAT 1 cut(s) 919
PagI TCATGA 2 cut(s) 133, 508
PctI GAATGC 1 cut(s) 917
PdmI GAANNNNTTC 2 cut(s) 708, 896
PfeI GAWTC 2 cut(s) 346, 776
PkrI GCNGC 4 cut(s) 145, 148, 393, 982
PleI GAGTC 1 cut(s) 845
PpsI GAGTC 1 cut(s) 845
PshBI ATTAAT 2 cut(s) 528, 558
Psp124BI GAGCTC 1 cut(s) 1041
PstI CTGCAG 1 cut(s) 148
RsaI GTAC 2 cut(s) 113, 538
RsaNI GTAC 2 cut(s) 112, 537
RseI CAYNNNNRTG 3 cut(s) 138, 326, 513
SacI GAGCTC 1 cut(s) 1041
SatI GCNGC 4 cut(s) 144, 147, 392, 981
Sau3AI GATC 3 cut(s) 33, 247, 577
SchI GAGTC 1 cut(s) 846
SduI GDGCHC 2 cut(s) 1026, 1041
SfaNI GCATC 4 cut(s) 148, 611, 904, 913
SfcI CTRYAG 1 cut(s) 144
SmiMI CAYNNNNRTG 3 cut(s) 138, 326, 513
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SsiI CCGC 2 cut(s) 77, 810
SspI AATATT 1 cut(s) 198
SspMI CTAG 2 cut(s) 278, 396
SstI GAGCTC 1 cut(s) 1041
TaaI ACNGT 2 cut(s) 515, 955
TaiI ACGT 1 cut(s) 13
TaqI TCGA 2 cut(s) 921, 1041
TatI WGTACW 1 cut(s) 536
TfiI GAWTC 2 cut(s) 346, 776
TscAI CASTG 2 cut(s) 520, 960
TseFI GTSAC 3 cut(s) 659, 838, 949
TseI GCWGC 4 cut(s) 143, 146, 391, 980
Tsp45I GTSAC 3 cut(s) 659, 838, 949
TspDTI ATGAA 5 cut(s) 150, 261, 310, 497, 768
TspGWI ACGGA 1 cut(s) 730
TspRI CASTG 2 cut(s) 520, 960
Vha464I CTTAAG 1 cut(s) 80
VspI ATTAAT 2 cut(s) 528, 558
XapI RAATTY 5 cut(s) 285, 365, 377, 551, 905
XbaI TCTAGA 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 690
XmnI GAANNNNTTC 2 cut(s) 708, 896
XspI CTAG 2 cut(s) 278, 396
Zsp2I ATGCAT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.