MD05G1129400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024466
Physical Location & Seq
Forward (+)
25331637 .. 25332207
571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1129400.v1.1.491

Sequence Viewer

Length: 345 bp
ATGTTGCATTTTGTGGAAGAAGACAGCAATCCGCCATCTCCAGCACATGAGTCACAGCAACCAACAACAACAGCATTTGGGAAGCCTATAGTCCCTGAGATCCCCGTGGTCTCAGAGGTAATTAGCCCACGGGCTTCTCCTCATCTAGCAACCATTACAGATGAGCTTCCTTCTTCTACTCAAGACCCTGCTCAGGCCTTTGGTGAGGGTTCGTGCACAATTCCTCATTCTCCAGTTAGTGGGAGTGATCCCCCTCCACAACCAATAGTCATTACCCCTCTTCCAAGGCCTCGGGCTCTATTCAGGACCCTGGAGAGGACTCGGGCAATTCCCCTCCACGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

115

Amino Acids

12.22

Weight (kDa)

5.38

Isoelectric Point (pI)

68.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024466)

Species Orthologous Gene IDs
malus_domestica MD05G1129400.v1.1
pyrus_communis pycom13g19760 pycom13g22580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 239
AciI CCGC 1 cut(s) 32
AclWI GGATC 2 cut(s) 94, 242
AfiI CCNNNNNNNGG 3 cut(s) 193, 239, 315
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 1 cut(s) 309
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 1 cut(s) 115
Alw44I GTGCAC 1 cut(s) 214
AlwI GGATC 2 cut(s) 94, 242
Ama87I CYCGRG 2 cut(s) 291, 321
AoxI GGCC 2 cut(s) 195, 287
ApaLI GTGCAC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 306
AsuHPI GGTGA 1 cut(s) 215
AvaI CYCGRG 2 cut(s) 291, 321
AvaII GGWCC 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 218
BccI CCATC 1 cut(s) 43
BciT130I CCWGG 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 115
BfaI CTAG 2 cut(s) 146, 343
BfmI CTRYAG 1 cut(s) 87
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 2 cut(s) 291, 321
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 306
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 3 cut(s) 24, 216, 332
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 5 cut(s) 105, 128, 284, 290, 309
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 239, 315
Bse1I ACTGG 1 cut(s) 233
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 5 cut(s) 105, 128, 284, 290, 309
BseLI CCNNNNNNNGG 3 cut(s) 193, 239, 315
BseMII CTCAG 3 cut(s) 87, 126, 206
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 129
BseSI GKGCMC 1 cut(s) 218
BshFI GGCC 2 cut(s) 197, 289
BsiHKAI GWGCWC 1 cut(s) 218
BsiHKCI CYCGRG 2 cut(s) 291, 321
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 193, 239, 315
BsmAI GTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 2 cut(s) 197, 289
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 2 cut(s) 291, 321
Bsp1286I GDGCHC 2 cut(s) 218, 298
Bsp143I GATC 2 cut(s) 99, 247
BspACI CCGC 1 cut(s) 32
BspANI GGCC 2 cut(s) 197, 289
BspCNI CTCAG 3 cut(s) 88, 125, 205
BspLI GGNNCC 1 cut(s) 308
BspPI GGATC 2 cut(s) 94, 242
BspTNI GGTCTC 1 cut(s) 115
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 5 cut(s) 105, 128, 284, 290, 309
BssMI GATC 2 cut(s) 99, 247
BssT1I CCWWGG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 285
BstDEI CTNAG 3 cut(s) 96, 112, 192
BstDSI CCRYGG 2 cut(s) 105, 128
BstKTI GATC 2 cut(s) 102, 250
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 2 cut(s) 99, 247
BstNI CCWGG 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 309
BstSFI CTRYAG 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 218
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BsuRI GGCC 2 cut(s) 197, 289
BtgI CCRYGG 2 cut(s) 105, 128
BtrI CACGTC 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 306
CviAII CATG 1 cut(s) 47
CviJI RGCY 7 cut(s) 85, 126, 134, 166, 197, 289, 296
CviKI_1 RGCY 7 cut(s) 85, 126, 134, 166, 197, 289, 296
DdeI CTNAG 3 cut(s) 96, 112, 192
DpnI GATC 2 cut(s) 101, 249
DpnII GATC 2 cut(s) 99, 247
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
EciI GGCGGA 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 284
Eco147I AGGCCT 2 cut(s) 197, 289
Eco24I GRGCYC 1 cut(s) 298
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 306
Eco88I CYCGRG 2 cut(s) 291, 321
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 284
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 284
FaeI CATG 1 cut(s) 50
FaiI YATR 2 cut(s) 48, 89
FaqI GGGAC 1 cut(s) 77
FatI CATG 1 cut(s) 46
FriOI GRGCYC 1 cut(s) 298
FspBI CTAG 2 cut(s) 146, 343
GsuI CTGGAG 3 cut(s) 24, 216, 332
HaeIII GGCC 2 cut(s) 197, 289
Hin1II CATG 1 cut(s) 50
HinfI GANTC 2 cut(s) 50, 319
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 2 cut(s) 182, 304
Hpy8I GTNNAC 1 cut(s) 216
HpyAV CCTTC 1 cut(s) 180
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 2 cut(s) 7, 216
HpyF3I CTNAG 3 cut(s) 96, 112, 192
HpySE526I ACGT 1 cut(s) 339
Hsp92II CATG 1 cut(s) 50
Kzo9I GATC 2 cut(s) 99, 247
LpnPI CCDG 8 cut(s) 54, 108, 179, 201, 246, 289, 296, 323
MaeI CTAG 2 cut(s) 146, 343
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 51
MalI GATC 2 cut(s) 101, 249
MboI GATC 2 cut(s) 99, 247
MboII GAAGA 4 cut(s) 29, 32, 165, 272
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 218, 298
MluCI AATT 3 cut(s) 120, 219, 327
MlyI GAGTC 2 cut(s) 59, 313
MnlI CCTC 9 cut(s) 109, 150, 199, 234, 264, 288, 300, 309, 344
MspR9I CCNGG 1 cut(s) 311
MvaI CCWGG 1 cut(s) 311
NdeII GATC 2 cut(s) 99, 247
NlaIII CATG 1 cut(s) 50
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 1 cut(s) 51
PceI AGGCCT 2 cut(s) 197, 289
PflMI CCANNNNNTGG 1 cut(s) 239
PleI GAGTC 2 cut(s) 58, 313
PpsI GAGTC 2 cut(s) 58, 313
PpuMI RGGWCCY 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 1 cut(s) 306
PspPPI RGGWCCY 1 cut(s) 306
PsuI RGATCY 1 cut(s) 99
Sau3AI GATC 2 cut(s) 99, 247
Sau96I GGNCC 1 cut(s) 306
SchI GAGTC 2 cut(s) 59, 313
ScrFI CCNGG 1 cut(s) 311
SduI GDGCHC 2 cut(s) 218, 298
SetI ASST 3 cut(s) 120, 168, 342
SfcI CTRYAG 1 cut(s) 87
SinI GGWCC 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
Sse9I AATT 3 cut(s) 120, 219, 327
SseBI AGGCCT 2 cut(s) 197, 289
SsiI CCGC 1 cut(s) 32
SspMI CTAG 2 cut(s) 146, 343
StuI AGGCCT 2 cut(s) 197, 289
StyD4I CCNGG 1 cut(s) 309
StyI CCWWGG 1 cut(s) 284
TaiI ACGT 1 cut(s) 342
TasI AATT 3 cut(s) 120, 219, 327
TseFI GTSAC 1 cut(s) 51
Tsp45I GTSAC 1 cut(s) 51
Van91I CCANNNNNTGG 1 cut(s) 239
VneI GTGCAC 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 306
XspI CTAG 2 cut(s) 146, 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.