MD05G1136400.v1.1

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0022425
Physical Location & Seq
Forward (+)
26452883 .. 26454734
1852 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1136400.v1.1.491

Sequence Viewer

Length: 774 bp
ATGTTCAGTCAGAAAAGCCTCAAAATGCGGATGACAAGGCCGTTGAAAATGGAGCAGCATATGATAAAAATGAAGGCAAGTCAGCAAAAGGTGCTCCGAACAGTCCATTTGCTAGCAGTACTGTTGGAAGGCCATCTAGAGAATTTTCAGATTCTAACTATGCAAAGACAACAGACGCAGATGCCTCACCCAGTAATAAGGAATCTCAAAGGTATAGTTCCGATGAACCAGCATGGGGGACATTTGACAATAATGATGACGTTGACTCGGTGTGGGGTTTCAGTGCAGTTAGTACCACCAAGGATATGGATCAGGAGTCACTACTTTTCTGGCCCTGGGGAGTTCGGCCTCAACCCTATCAGGACTGGGTCATCACAAGGAGGTGGCTTTTCCCAGAAGAGCCGCCCTTTCACTTTCGACGATTCTGTTCCTAGCACACCACTCTCAGCCTTCAATTCAGGGTATTCACCGCCAAGGTACAAGGATAGCTCAGAACCTTCATTTGACACCTTCTCTAGGTTTGATTCGTTCAGAATCACACAAGATACTGGATTTTTTCCTCAACTAGAGGCACTCGGAAGATTTGATTCTATGCGCAGCAGTAGAGATTTTGATCAGGGTCATGCTTTTCCAACATTTGATGACATCCCAGACCCTTTTGGCTCTTCGGTGCCATTTAGGTCATCGCTGGACAATCAAACTCCAAGAAGAGACTCAGACCCCTTCGGATCTTCAGGTCCATTTCGGACGTCACTGGACAGTCAAACTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

258

Amino Acids

29.04

Weight (kDa)

9.85

Isoelectric Point (pI)

57.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022425)

Species Orthologous Gene IDs
malus_domestica MD05G1136400.v1.1 MD05G1136500.v1.1
pyrus_communis pycom05g13250
rosa_multiflora Rmu_sc0011374.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 752
Acc16I TGCGCA 1 cut(s) 596
AccB1I GGYRCC 1 cut(s) 670
AciI CCGC 3 cut(s) 28, 403, 470
AclWI GGATC 2 cut(s) 317, 736
AcsI RAATTY 1 cut(s) 142
AcuI CTGAAG 1 cut(s) 717
AcyI GRCGYC 1 cut(s) 749
AfaI GTAC 3 cut(s) 120, 294, 479
AfiI CCNNNNNNNGG 2 cut(s) 235, 516
AgsI TTSAA 2 cut(s) 46, 454
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 1 cut(s) 489
AluI AGCT 1 cut(s) 489
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 705
AlwI GGATC 2 cut(s) 317, 736
AoxI GGCC 4 cut(s) 38, 130, 331, 346
ApeKI GCWGC 2 cut(s) 55, 597
ApoI RAATTY 1 cut(s) 142
AspLEI GCGC 1 cut(s) 597
AspS9I GGNCC 2 cut(s) 332, 737
AsuHPI GGTGA 2 cut(s) 179, 459
AsuNHI GCTAGC 1 cut(s) 112
AvaII GGWCC 1 cut(s) 737
BanI GGYRCC 1 cut(s) 670
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 67, 609
BccI CCATC 1 cut(s) 141
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 1 cut(s) 336
BclI TGATCA 1 cut(s) 613
BcoDI GTCTC 1 cut(s) 705
BfaI CTAG 5 cut(s) 113, 137, 432, 516, 566
BisI GCNGC 3 cut(s) 56, 403, 598
BlsI GCNGC 3 cut(s) 57, 404, 599
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 737
BmgT120I GGNCC 2 cut(s) 332, 737
BmiI GGNNCC 1 cut(s) 672
BmrFI CCNGG 1 cut(s) 336
BmrI ACTGGG 2 cut(s) 185, 375
BmsI GCATC 1 cut(s) 171
BmtI GCTAGC 1 cut(s) 116
BmuI ACTGGG 2 cut(s) 185, 375
BsaBI GATNNNNATC 2 cut(s) 308, 612
BsaHI GRCGYC 1 cut(s) 749
BsaJI CCNNGG 4 cut(s) 299, 334, 335, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 516
Bse1I ACTGG 4 cut(s) 191, 370, 553, 759
Bse8I GATNNNNATC 2 cut(s) 308, 612
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 4 cut(s) 299, 334, 335, 473
BseGI GGATG 2 cut(s) 36, 645
BseJI GATNNNNATC 2 cut(s) 308, 612
BseLI CCNNNNNNNGG 2 cut(s) 235, 516
BseMII CTCAG 3 cut(s) 459, 504, 729
BseNI ACTGG 4 cut(s) 191, 370, 553, 759
BseXI GCAGC 2 cut(s) 67, 609
BsgI GTGCAG 1 cut(s) 305
BshFI GGCC 4 cut(s) 40, 132, 333, 348
BshNI GGYRCC 1 cut(s) 670
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 2 cut(s) 235, 516
BsmAI GTCTC 1 cut(s) 705
BsmFI GGGAC 1 cut(s) 252
BsnI GGCC 4 cut(s) 40, 132, 333, 348
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 3 cut(s) 309, 613, 728
BspACI CCGC 3 cut(s) 28, 403, 470
BspANI GGCC 4 cut(s) 40, 132, 333, 348
BspCNI CTCAG 3 cut(s) 458, 503, 728
BspLI GGNNCC 1 cut(s) 672
BspOI GCTAGC 1 cut(s) 116
BspPI GGATC 2 cut(s) 317, 736
BspQI GCTCTTC 2 cut(s) 392, 670
BspT107I GGYRCC 1 cut(s) 670
BsrI ACTGG 4 cut(s) 191, 370, 553, 759
BssECI CCNNGG 4 cut(s) 299, 334, 335, 473
BssMI GATC 3 cut(s) 309, 613, 728
BssNI GRCGYC 1 cut(s) 749
BssT1I CCWWGG 2 cut(s) 299, 473
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 3 cut(s) 103, 123, 761
Bst6I CTCTTC 3 cut(s) 392, 670, 703
BstACI GRCGYC 1 cut(s) 749
BstAPI GCANNNNNTGC 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 3 cut(s) 445, 490, 715
BstENI CCTNNNNNAGG 1 cut(s) 514
BstF5I GGATG 2 cut(s) 36, 645
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 3 cut(s) 312, 616, 731
BstMAI GTCTC 1 cut(s) 705
BstMBI GATC 3 cut(s) 309, 613, 728
BstMWI GCNNNNNNNGC 1 cut(s) 91
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 67, 609
BstX2I RGATCY 1 cut(s) 728
BstXI CCANNNNNNTGG 1 cut(s) 306
BstYI RGATCY 1 cut(s) 728
BsuRI GGCC 4 cut(s) 40, 132, 333, 348
BtgZI GCGATG 1 cut(s) 669
BtsCI GGATG 2 cut(s) 36, 645
BtsIMutI CAGTG 2 cut(s) 288, 752
Cac8I GCNNGC 1 cut(s) 114
CfoI GCGC 1 cut(s) 597
Cfr13I GGNCC 2 cut(s) 332, 737
CseI GACGC 1 cut(s) 184
Csp6I GTAC 3 cut(s) 119, 293, 478
CviAII CATG 2 cut(s) 233, 623
CviQI GTAC 3 cut(s) 119, 293, 478
DdeI CTNAG 3 cut(s) 445, 490, 715
DpnI GATC 3 cut(s) 311, 615, 730
DpnII GATC 3 cut(s) 309, 613, 728
Eam1104I CTCTTC 3 cut(s) 392, 670, 703
EarI CTCTTC 3 cut(s) 392, 670, 703
Eco130I CCWWGG 2 cut(s) 299, 473
Eco47I GGWCC 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 717
EcoNI CCTNNNNNAGG 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 299, 473
ErhI CCWWGG 2 cut(s) 299, 473
FaeI CATG 2 cut(s) 236, 626
FaiI YATR 8 cut(s) 60, 62, 161, 215, 234, 307, 593, 624
FaqI GGGAC 1 cut(s) 252
FatI CATG 2 cut(s) 232, 622
FauNDI CATATG 1 cut(s) 60
FbaI TGATCA 1 cut(s) 613
Fnu4HI GCNGC 3 cut(s) 56, 403, 598
FokI GGATG 2 cut(s) 43, 632
Fsp4HI GCNGC 3 cut(s) 56, 403, 598
FspBI CTAG 5 cut(s) 113, 137, 432, 516, 566
FspI TGCGCA 1 cut(s) 596
GlaI GCGC 1 cut(s) 596
GluI GCNGC 3 cut(s) 56, 403, 598
HaeIII GGCC 4 cut(s) 40, 132, 333, 348
HgaI GACGC 1 cut(s) 184
HhaI GCGC 1 cut(s) 597
Hin1I GRCGYC 1 cut(s) 749
Hin1II CATG 2 cut(s) 236, 626
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HinfI GANTC 9 cut(s) 151, 202, 265, 316, 422, 524, 534, 587, 713
HphI GGTGA 2 cut(s) 179, 459
Hpy166II GTNNAC 1 cut(s) 264
Hpy188III TCNNGA 3 cut(s) 137, 313, 361
Hpy8I GTNNAC 1 cut(s) 264
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 6 cut(s) 67, 122, 460, 507, 520, 733
HpyCH4III ACNGT 3 cut(s) 103, 123, 761
HpyCH4IV ACGT 2 cut(s) 260, 749
HpyCH4V TGCA 2 cut(s) 163, 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 91
HpyF3I CTNAG 3 cut(s) 445, 490, 715
HpySE526I ACGT 2 cut(s) 260, 749
Hsp92I GRCGYC 1 cut(s) 749
Hsp92II CATG 2 cut(s) 236, 626
HspAI GCGC 1 cut(s) 595
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 3 cut(s) 309, 613, 728
LguI GCTCTTC 2 cut(s) 392, 670
LmnI GCTCC 2 cut(s) 52, 99
Lsp1109I GCAGC 2 cut(s) 67, 609
LweI GCATC 1 cut(s) 171
MaeI CTAG 5 cut(s) 113, 137, 432, 516, 566
MaeII ACGT 2 cut(s) 260, 749
MaeIII GTNAC 2 cut(s) 317, 750
MalI GATC 3 cut(s) 311, 615, 730
MboI GATC 3 cut(s) 309, 613, 728
MboII GAAGA 5 cut(s) 409, 591, 657, 720, 723
MflI RGATCY 1 cut(s) 728
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 2 cut(s) 142, 454
MlyI GAGTC 3 cut(s) 259, 325, 707
MmeI TCCRAC 2 cut(s) 105, 656
MnlI CCTC 6 cut(s) 29, 195, 359, 374, 562, 570
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 91
NdeI CATATG 1 cut(s) 60
NdeII GATC 3 cut(s) 309, 613, 728
NheI GCTAGC 1 cut(s) 112
NlaIII CATG 2 cut(s) 236, 626
NlaIV GGNNCC 1 cut(s) 672
NmuCI GTSAC 2 cut(s) 317, 750
NsbI TGCGCA 1 cut(s) 596
PasI CCCWGGG 1 cut(s) 335
PciSI GCTCTTC 2 cut(s) 392, 670
PfeI GAWTC 6 cut(s) 151, 202, 422, 524, 534, 587
PflFI GACNNNGTC 1 cut(s) 367
PkrI GCNGC 3 cut(s) 57, 404, 599
PleI GAGTC 3 cut(s) 259, 324, 707
PpsI GAGTC 3 cut(s) 259, 324, 707
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 672
PspPI GGNCC 2 cut(s) 332, 737
PsuI RGATCY 1 cut(s) 728
PsyI GACNNNGTC 1 cut(s) 367
RsaI GTAC 3 cut(s) 120, 294, 479
RsaNI GTAC 3 cut(s) 119, 293, 478
SapI GCTCTTC 2 cut(s) 392, 670
SatI GCNGC 3 cut(s) 56, 403, 598
Sau3AI GATC 3 cut(s) 309, 613, 728
Sau96I GGNCC 2 cut(s) 332, 737
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 3 cut(s) 259, 325, 707
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 1 cut(s) 171
SinI GGWCC 1 cut(s) 737
Sse9I AATT 2 cut(s) 142, 454
SsiI CCGC 3 cut(s) 28, 403, 470
SspMI CTAG 5 cut(s) 113, 137, 432, 516, 566
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 2 cut(s) 299, 473
TaaI ACNGT 3 cut(s) 103, 123, 761
TaiI ACGT 2 cut(s) 263, 752
TaqI TCGA 1 cut(s) 417
TasI AATT 2 cut(s) 142, 454
TatI WGTACW 1 cut(s) 118
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 6 cut(s) 151, 202, 422, 524, 534, 587
TscAI CASTG 2 cut(s) 288, 759
TseFI GTSAC 2 cut(s) 317, 750
TseI GCWGC 2 cut(s) 55, 597
Tsp45I GTSAC 2 cut(s) 317, 750
TspDTI ATGAA 3 cut(s) 86, 239, 489
TspGWI ACGGA 1 cut(s) 759
TspRI CASTG 2 cut(s) 288, 759
Tth111I GACNNNGTC 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 737
XagI CCTNNNNNAGG 1 cut(s) 514
XapI RAATTY 1 cut(s) 142
XbaI TCTAGA 1 cut(s) 136
XcmI CCANNNNNNNNNTGG 1 cut(s) 303
XspI CTAG 5 cut(s) 113, 137, 432, 516, 566
ZraI GACGTC 1 cut(s) 750
ZrmI AGTACT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.