MD05G1136500.v1.1

Epidermal growth factor receptor substrate 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0022425
Physical Location & Seq
Forward (+)
26468183 .. 26472208
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1136500.v1.1.491

Sequence Viewer

Length: 492 bp
ATGCCAAGGTTGTTGAAAATGGAGCAGCATATGATAAAAATGAGGATGAGTCAGCAAAAAGTGCTCCGAACAGTCCATTTGCCAGCAGTACCGTTGGAAGCCCATCTAGAGAATTTTCAGATTCTAACTATGGAAAGACAACAGACGCAGATCCCTCACCCCGTAATAAGGAATCTCAAAGGTTCTGATGAACCAGCATGGGGGACATTTGACACTAATGATGACGTTGACTCGTTGTGGGGTTTCAATGCAGTTAGTACCACCAAGGAGAGTAATAAGGATCACTACTTTTCTGGCCCTGGGGAGTTCGGCCTCAACCCTATCAGGACTGGGTCATCACAAGGAGGTGGCTTTTCCCAGAAGAGCTACCCTTTTACTTTCGACGTTTCTGTTCCTAGCACACCACTCTCATCCTTCAATTTGGGGTATTCACCGCCAAGGTACAAGGATAGCTCAGAACCTTCATTTGACACCTTCTCTAGGTTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_4 EF-hand_5

Protein Analysis

164

Amino Acids

18.45

Weight (kDa)

6.19

Isoelectric Point (pI)

56.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022425)

Species Orthologous Gene IDs
malus_domestica MD05G1136400.v1.1 MD05G1136500.v1.1
pyrus_communis pycom05g13250
rosa_multiflora Rmu_sc0011374.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 434
AclWI GGATC 2 cut(s) 145, 288
AcsI RAATTY 1 cut(s) 112
AfaI GTAC 3 cut(s) 90, 259, 443
AfiI CCNNNNNNNGG 3 cut(s) 168, 200, 480
AgsI TTSAA 3 cut(s) 16, 247, 418
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 2 cut(s) 366, 453
AluI AGCT 2 cut(s) 366, 453
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 2 cut(s) 145, 288
AoxI GGCC 2 cut(s) 295, 310
ApeKI GCWGC 1 cut(s) 25
ApoI RAATTY 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 149, 423
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 37
BccI CCATC 1 cut(s) 111
BciT130I CCWGG 1 cut(s) 300
BfaI CTAG 3 cut(s) 107, 396, 480
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
Bme1390I CCNGG 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 296
BmrFI CCNGG 1 cut(s) 300
BmrI ACTGGG 1 cut(s) 339
BmuI ACTGGG 1 cut(s) 339
BsaJI CCNNGG 5 cut(s) 5, 264, 298, 299, 437
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 200, 480
Bse1I ACTGG 1 cut(s) 334
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 5 cut(s) 5, 264, 298, 299, 437
BseGI GGATG 2 cut(s) 51, 410
BseLI CCNNNNNNNGG 3 cut(s) 168, 200, 480
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 1 cut(s) 334
BseXI GCAGC 1 cut(s) 37
BshFI GGCC 2 cut(s) 297, 312
BsiHKAI GWGCWC 1 cut(s) 66
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 3 cut(s) 168, 200, 480
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 2 cut(s) 297, 312
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 2 cut(s) 150, 280
BspACI CCGC 1 cut(s) 434
BspANI GGCC 2 cut(s) 297, 312
BspCNI CTCAG 1 cut(s) 467
BspPI GGATC 2 cut(s) 145, 288
BspQI GCTCTTC 1 cut(s) 356
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 5 cut(s) 5, 264, 298, 299, 437
BssMI GATC 2 cut(s) 150, 280
BssT1I CCWWGG 3 cut(s) 5, 264, 437
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 73, 93
Bst6I CTCTTC 1 cut(s) 356
BstAPI GCANNNNNTGC 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 1 cut(s) 454
BstENI CCTNNNNNAGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 51, 410
BstKTI GATC 2 cut(s) 153, 283
BstMBI GATC 2 cut(s) 150, 280
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstV1I GCAGC 1 cut(s) 37
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 2 cut(s) 297, 312
BtsCI GGATG 2 cut(s) 51, 410
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 154
Csp6I GTAC 3 cut(s) 89, 258, 442
CviAII CATG 1 cut(s) 198
CviJI RGCY 6 cut(s) 101, 297, 312, 351, 366, 453
CviKI_1 RGCY 6 cut(s) 101, 297, 312, 351, 366, 453
CviQI GTAC 3 cut(s) 89, 258, 442
DdeI CTNAG 1 cut(s) 454
DpnI GATC 2 cut(s) 152, 282
DpnII GATC 2 cut(s) 150, 280
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco130I CCWWGG 3 cut(s) 5, 264, 437
EcoNI CCTNNNNNAGG 1 cut(s) 478
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 3 cut(s) 5, 264, 437
ErhI CCWWGG 3 cut(s) 5, 264, 437
FaeI CATG 1 cut(s) 201
FaiI YATR 4 cut(s) 30, 32, 131, 199
FaqI GGGAC 1 cut(s) 217
FatI CATG 1 cut(s) 197
FauNDI CATATG 1 cut(s) 30
Fnu4HI GCNGC 1 cut(s) 26
FokI GGATG 2 cut(s) 58, 397
Fsp4HI GCNGC 1 cut(s) 26
FspBI CTAG 3 cut(s) 107, 396, 480
GluI GCNGC 1 cut(s) 26
HaeIII GGCC 2 cut(s) 297, 312
HgaI GACGC 1 cut(s) 154
Hin1II CATG 1 cut(s) 201
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 4 cut(s) 49, 121, 172, 230
HphI GGTGA 2 cut(s) 149, 423
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 4 cut(s) 68, 120, 187, 457
Hpy188III TCNNGA 2 cut(s) 107, 325
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 3 cut(s) 424, 471, 484
HpyCH4III ACNGT 2 cut(s) 73, 93
HpyCH4IV ACGT 2 cut(s) 225, 384
HpyCH4V TGCA 1 cut(s) 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 1 cut(s) 454
HpySE526I ACGT 2 cut(s) 225, 384
Hsp92II CATG 1 cut(s) 201
Kzo9I GATC 2 cut(s) 150, 280
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 2 cut(s) 22, 69
LpnPI CCDG 8 cut(s) 96, 207, 279, 285, 310, 312, 315, 371
Lsp1109I GCAGC 1 cut(s) 37
MaeI CTAG 3 cut(s) 107, 396, 480
MaeII ACGT 2 cut(s) 225, 384
MalI GATC 2 cut(s) 152, 282
MboI GATC 2 cut(s) 150, 280
MboII GAAGA 1 cut(s) 373
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 2 cut(s) 112, 418
MlyI GAGTC 2 cut(s) 58, 224
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 4 cut(s) 36, 165, 323, 338
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 61
NdeI CATATG 1 cut(s) 30
NdeII GATC 2 cut(s) 150, 280
NlaIII CATG 1 cut(s) 201
PasI CCCWGGG 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 121, 172
PflFI GACNNNGTC 1 cut(s) 331
PkrI GCNGC 1 cut(s) 27
PleI GAGTC 2 cut(s) 57, 224
PpsI GAGTC 2 cut(s) 57, 224
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspPI GGNCC 1 cut(s) 296
PsuI RGATCY 1 cut(s) 150
PsyI GACNNNGTC 1 cut(s) 331
RsaI GTAC 3 cut(s) 90, 259, 443
RsaNI GTAC 3 cut(s) 89, 258, 442
SapI GCTCTTC 1 cut(s) 356
SatI GCNGC 1 cut(s) 26
Sau3AI GATC 2 cut(s) 150, 280
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 2 cut(s) 58, 224
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 1 cut(s) 66
Sse9I AATT 2 cut(s) 112, 418
SsiI CCGC 1 cut(s) 434
SspMI CTAG 3 cut(s) 107, 396, 480
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 3 cut(s) 5, 264, 437
TaaI ACNGT 2 cut(s) 73, 93
TaiI ACGT 2 cut(s) 228, 387
TaqI TCGA 1 cut(s) 381
TasI AATT 2 cut(s) 112, 418
TfiI GAWTC 2 cut(s) 121, 172
TseI GCWGC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 204, 453
Tth111I GACNNNGTC 1 cut(s) 331
XagI CCTNNNNNAGG 1 cut(s) 478
XapI RAATTY 1 cut(s) 112
XbaI TCTAGA 1 cut(s) 106
XspI CTAG 3 cut(s) 107, 396, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.