MD05G1146100.v1.1

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0020396
Physical Location & Seq
Reverse (-)
27702606 .. 27704077
1472 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1146100.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCGTTGGCATATCCTGAAATTCTTTATCAGGGTTTCGAGAATGTTGACATTTTGCTGGAATCTCGACCACTTGAAAAAGGCCTCTCAGCTGATCAGTTTCTTCAGTTGGTAGTGGTCAAGATAGGTGCATCCTCATTGTGTCGAGCCTCTGGATTAGTTGGAGGCTACTATGCTCCATCACTTTTAACTGGTGCAGCAACAGGAATGACATACGGAAAATTCATCAGTTCTGCAGTTGTCAGTCCAATCCTATACTTCATCTTTTCCATATTGGAAGTAGCATCACCGCAAGCATACAGCCTGTCTTGCAGGAGTCTGTTGCTTTTTGAATTGACACAAGATTATCGTATTGTGCTGCTGCTACTTGGAGCTGTGGGGTTATCTTCGTGGATTACATCTGGACAGACGAGGAGAAAAGTAATCGGGAAGACTAAGAAACTCAAACAGGGAAACACTAGTAGCATTCAACAACCTGAAGTGCTTTCCCCACTGCAAATGGGATATCCTCAAGTGGTGGTTCAGCTGAAAGGATATCTAATGCAAGTGACCTGTGTGAAATTGAAAGTTCACTCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS Voltage_CLC

Protein Analysis

194

Amino Acids

20.98

Weight (kDa)

9.36

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voltage_CLC PF00654 2 - 133 3.2e-15 Voltage gated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020396)

Species Orthologous Gene IDs
malus_domestica MD05G1146100.v1.1 MD05G1149100.v1.1 MD05G1150500.v1.1
pyrus_communis pycom05g14230 pycom05g14270 pycom05g14400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 290
AcsI RAATTY 2 cut(s) 21, 221
AcuI CTGAAG 2 cut(s) 89, 498
AgsI TTSAA 4 cut(s) 77, 332, 470, 565
AhlI ACTAGT 1 cut(s) 458
AluBI AGCT 3 cut(s) 92, 374, 526
AluI AGCT 3 cut(s) 92, 374, 526
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 3 cut(s) 197, 358, 361
ApoI RAATTY 2 cut(s) 21, 221
AsuHPI GGTGA 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 437
BbvI GCAGC 3 cut(s) 209, 345, 348
BccI CCATC 1 cut(s) 187
BclI TGATCA 1 cut(s) 94
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 1 cut(s) 459
BfmI CTRYAG 1 cut(s) 234
BisI GCNGC 3 cut(s) 198, 359, 362
BlsI GCNGC 3 cut(s) 199, 360, 363
BmsI GCATC 2 cut(s) 140, 293
BpiI GAAGAC 1 cut(s) 437
BpuEI CTTGAG 1 cut(s) 495
Bse1I ACTGG 1 cut(s) 196
BseGI GGATG 1 cut(s) 131
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 196
BseRI GAGGAG 1 cut(s) 427
BseXI GCAGC 3 cut(s) 209, 345, 348
BsgI GTGCAG 1 cut(s) 216
BshFI GGCC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 465
BsnI GGCC 1 cut(s) 84
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 1 cut(s) 290
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 1 cut(s) 101
BspMAI CTGCAG 1 cut(s) 238
BsrI ACTGG 1 cut(s) 196
BssMI GATC 1 cut(s) 94
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 2 cut(s) 88, 435
BstF5I GGATG 1 cut(s) 131
BstKTI GATC 1 cut(s) 97
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 1 cut(s) 309
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 3 cut(s) 209, 345, 348
BstV2I GAAGAC 1 cut(s) 437
BsuRI GGCC 1 cut(s) 84
BtsCI GGATG 1 cut(s) 131
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 1 cut(s) 491
Cac8I GCNNGC 1 cut(s) 294
CviJI RGCY 7 cut(s) 84, 92, 149, 168, 303, 374, 526
CviKI_1 RGCY 7 cut(s) 84, 92, 149, 168, 303, 374, 526
DdeI CTNAG 2 cut(s) 88, 435
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
Eco147I AGGCCT 1 cut(s) 84
Eco32I GATATC 2 cut(s) 506, 536
Eco57I CTGAAG 2 cut(s) 89, 498
EcoRV GATATC 2 cut(s) 506, 536
FaiI YATR 7 cut(s) 13, 174, 214, 256, 272, 298, 580
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 198, 359, 362
FokI GGATG 1 cut(s) 118
Fsp4HI GCNGC 3 cut(s) 198, 359, 362
FspBI CTAG 1 cut(s) 459
GluI GCNGC 3 cut(s) 198, 359, 362
HaeIII GGCC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 2 cut(s) 62, 316
HphI GGTGA 1 cut(s) 279
Hpy166II GTNNAC 2 cut(s) 49, 571
Hpy188III TCNNGA 7 cut(s) 17, 40, 66, 121, 153, 402, 427
Hpy8I GTNNAC 2 cut(s) 49, 571
HpyCH4V TGCA 6 cut(s) 131, 197, 236, 312, 496, 544
HpyF10VI GCNNNNNNNGC 1 cut(s) 309
HpyF3I CTNAG 2 cut(s) 88, 435
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 181, 371
Lsp1109I GCAGC 3 cut(s) 209, 345, 348
LweI GCATC 2 cut(s) 140, 293
MaeI CTAG 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 547
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 3 cut(s) 95, 378, 442
MluCI AATT 4 cut(s) 21, 221, 332, 560
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 6 cut(s) 95, 145, 158, 160, 405, 519
MseI TTAA 1 cut(s) 188
MspA1I CMGCKG 2 cut(s) 92, 526
Mva1269I GAATGC 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 309
NdeII GATC 1 cut(s) 94
NmuCI GTSAC 1 cut(s) 547
PceI AGGCCT 1 cut(s) 84
PctI GAATGC 1 cut(s) 465
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 3 cut(s) 199, 360, 363
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
PstI CTGCAG 1 cut(s) 238
PvuII CAGCTG 2 cut(s) 92, 526
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 3 cut(s) 198, 359, 362
Sau3AI GATC 1 cut(s) 94
SchI GAGTC 1 cut(s) 325
SetI ASST 6 cut(s) 94, 130, 376, 478, 528, 554
SfaNI GCATC 2 cut(s) 140, 293
SfcI CTRYAG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 510
SmoI CTYRAG 1 cut(s) 510
SpeI ACTAGT 1 cut(s) 458
Sse9I AATT 4 cut(s) 21, 221, 332, 560
SseBI AGGCCT 1 cut(s) 84
SsiI CCGC 1 cut(s) 290
SspMI CTAG 1 cut(s) 459
StuI AGGCCT 1 cut(s) 84
TaqI TCGA 3 cut(s) 39, 67, 145
TasI AATT 4 cut(s) 21, 221, 332, 560
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TscAI CASTG 1 cut(s) 498
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 3 cut(s) 197, 358, 361
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 2 cut(s) 214, 250
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 1 cut(s) 498
XapI RAATTY 2 cut(s) 21, 221
XspI CTAG 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.