MD05G1150500.v1.1

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0020396
Physical Location & Seq
Reverse (-)
28138648 .. 28139359
712 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1150500.v1.1.491

Sequence Viewer

Length: 564 bp
ATGTTCAAGTTATTGTTTAAAGCTGCGGTTTTAATAATGAGACGTATTCGAATCATGTTCAGAGCTTCCGCTATATCTATTGCTGGGTGTCCTATGTGGCTTGGTTCGTTAACCTTATCAAAGTGCAAATCGTACATGTTGGTATTTGTTGACAATCTGCACAAGACTGTTGGCCTACCAAAAATTTTGTTCCCTATCGTGGGTGGCTTGGCTATTGGGCTGATGGCATTGGCATATCCTGAAATTCTTTATCCGGGTTTCAAGAGTGTTGACATTTTGCTGGAATCTCGACCACTTGAGAAAGGCCTCTCAGCTGATCTGTTGCTTCAGTTGGTAGTGGTCAAGATAGGTGCAACCTCATTGTGTCGAGCCTCTGGATTAGTTGGAGGCTACCATGCTCCATCACTTTTTATTTGTGCAGCAACAGGAATGTCATACGGCAAAAACATCAGTTCTGCAGTTGCTCAGTCCAATCCTATACTTCATCTTTCTGTCTTGGAAGTAGCATCATCGCAAGCATACGGCCTGGTAATGTTTTATTTTTTTCCTATATATTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS Voltage_CLC

Protein Analysis

188

Amino Acids

20.27

Weight (kDa)

9.54

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voltage_CLC PF00654 45 - 157 5.3e-16 Voltage gated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020396)

Species Orthologous Gene IDs
malus_domestica MD05G1146100.v1.1 MD05G1149100.v1.1 MD05G1150500.v1.1
pyrus_communis pycom05g14230 pycom05g14270 pycom05g14400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 26, 69
AcsI RAATTY 2 cut(s) 183, 243
AcuI CTGAAG 1 cut(s) 311
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 2 cut(s) 199, 200
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 2 cut(s) 7, 262
AjnI CCWGG 1 cut(s) 525
AluBI AGCT 3 cut(s) 23, 65, 314
AluI AGCT 3 cut(s) 23, 65, 314
Alw26I GTCTC 1 cut(s) 34
AoxI GGCC 3 cut(s) 172, 304, 523
ApeKI GCWGC 2 cut(s) 23, 419
ApoI RAATTY 2 cut(s) 183, 243
AsuC2I CCSGG 1 cut(s) 255
AsuII TTCGAA 1 cut(s) 49
BbvI GCAGC 2 cut(s) 10, 431
BccI CCATC 2 cut(s) 217, 409
BceAI ACGGC 2 cut(s) 454, 538
BciT130I CCWGG 1 cut(s) 527
BcnI CCSGG 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 34
BfmI CTRYAG 1 cut(s) 456
BisI GCNGC 2 cut(s) 24, 420
BlsI GCNGC 2 cut(s) 25, 421
Bme1390I CCNGG 2 cut(s) 255, 527
BmrFI CCNGG 2 cut(s) 255, 527
BmsI GCATC 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 49
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 200
BseBI CCWGG 1 cut(s) 527
BseLI CCNNNNNNNGG 2 cut(s) 199, 200
BseMII CTCAG 2 cut(s) 324, 479
BseXI GCAGC 2 cut(s) 10, 431
BseYI CCCAGC 1 cut(s) 83
BsgI GTGCAG 2 cut(s) 143, 438
BshFI GGCC 3 cut(s) 174, 306, 525
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 199, 200
BsmAI GTCTC 1 cut(s) 34
BsmBI CGTCTC 1 cut(s) 34
BsnI GGCC 3 cut(s) 174, 306, 525
Bsp119I TTCGAA 1 cut(s) 49
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 2 cut(s) 26, 69
BspANI GGCC 3 cut(s) 174, 306, 525
BspCNI CTCAG 2 cut(s) 323, 478
BspMAI CTGCAG 1 cut(s) 460
BspT104I TTCGAA 1 cut(s) 49
BssMI GATC 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 1 cut(s) 169
BstBI TTCGAA 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 2 cut(s) 310, 465
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 1 cut(s) 316
BstNI CCWGG 1 cut(s) 527
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 2 cut(s) 253, 525
BstSFI CTRYAG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 10, 431
BsuRI GGCC 3 cut(s) 174, 306, 525
BtgZI GCGATG 1 cut(s) 495
Cac8I GCNNGC 1 cut(s) 516
Csp6I GTAC 1 cut(s) 133
CviAII CATG 3 cut(s) 55, 136, 395
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 2 cut(s) 310, 465
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
DraI TTTAAA 1 cut(s) 19
Eco147I AGGCCT 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 525
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 3 cut(s) 58, 139, 398
FatI CATG 3 cut(s) 54, 135, 394
Fnu4HI GCNGC 2 cut(s) 24, 420
Fsp4HI GCNGC 2 cut(s) 24, 420
GluI GCNGC 2 cut(s) 24, 420
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 3 cut(s) 174, 306, 525
HapII CCGG 1 cut(s) 254
Hin1II CATG 3 cut(s) 58, 139, 398
HincII GTYRAC 3 cut(s) 111, 151, 271
HindII GTYRAC 3 cut(s) 111, 151, 271
HinfI GANTC 2 cut(s) 51, 284
HpaI GTTAAC 1 cut(s) 111
HpaII CCGG 1 cut(s) 254
Hpy166II GTNNAC 3 cut(s) 111, 151, 271
Hpy188I TCNGA 1 cut(s) 62
Hpy188III TCNNGA 5 cut(s) 239, 262, 288, 343, 375
Hpy8I GTNNAC 3 cut(s) 111, 151, 271
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 5 cut(s) 126, 160, 353, 419, 458
HpyF3I CTNAG 2 cut(s) 310, 465
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 3 cut(s) 58, 139, 398
KspAI GTTAAC 1 cut(s) 111
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 1 cut(s) 403
LpnPI CCDG 8 cut(s) 69, 252, 266, 267, 360, 411, 512, 539
Lsp1109I GCAGC 2 cut(s) 10, 431
LweI GCATC 1 cut(s) 515
MaeII ACGT 1 cut(s) 43
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MluCI AATT 2 cut(s) 183, 243
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 4 cut(s) 317, 367, 380, 382
MseI TTAA 3 cut(s) 18, 32, 110
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 255, 527
MvaI CCWGG 1 cut(s) 527
NciI CCSGG 1 cut(s) 255
NdeII GATC 1 cut(s) 316
NlaIII CATG 3 cut(s) 58, 139, 398
NspI RCATGY 1 cut(s) 139
NspV TTCGAA 1 cut(s) 49
PceI AGGCCT 1 cut(s) 306
PciI ACATGT 1 cut(s) 135
PfeI GAWTC 2 cut(s) 51, 284
PkrI GCNGC 2 cut(s) 25, 421
PscI ACATGT 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 525
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 525
PstI CTGCAG 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
SaqAI TTAA 3 cut(s) 18, 32, 110
SatI GCNGC 2 cut(s) 24, 420
Sau3AI GATC 1 cut(s) 316
ScrFI CCNGG 2 cut(s) 255, 527
SetI ASST 7 cut(s) 25, 46, 67, 116, 316, 352, 359
SfaNI GCATC 1 cut(s) 515
SfcI CTRYAG 1 cut(s) 456
SfuI TTCGAA 1 cut(s) 49
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 2 cut(s) 183, 243
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 2 cut(s) 26, 69
StuI AGGCCT 1 cut(s) 306
StyD4I CCNGG 2 cut(s) 253, 525
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 46
TaqI TCGA 3 cut(s) 49, 289, 367
TasI AATT 2 cut(s) 183, 243
TfiI GAWTC 2 cut(s) 51, 284
Tru1I TTAA 3 cut(s) 18, 32, 110
Tru9I TTAA 3 cut(s) 18, 32, 110
TseI GCWGC 2 cut(s) 23, 419
TspDTI ATGAA 1 cut(s) 473
XapI RAATTY 2 cut(s) 183, 243
XceI RCATGY 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.