MD05G1155300.v1.1

FAR1 DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0022289
Physical Location & Seq
Forward (+)
28530207 .. 28532177
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1155300.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGAGTCTGAAGCGGATTTGAACAAGCCGGTTGTGGAGAGTTCAGTTGAAAGGGATGTTCCAAACAATGAAAGTGGAGAAATGCTAGATTCAAACAATGGGACAGAGTTGATTGAAAATGATGTAAATGGTATCCTAGAACCTTATGTTGGAATGGAGTTTGACTCCGAAGAGGCTGTCAAGGAATACTATGATGCATATGCCACTCGTGTGGGTTTTAGAATCCGTGTTGGTAACTTCCATCGTTCAAATCGTGATGGATCCATCAACAGTCGTAAGTTTCTTTGTAACAAAGAAGGTTTTCGTAACAATAAGAAGTCAAAGAGGGGTGAAGTAAGGAGGTCGAGGGAGGAAACTAGGGAGGGCTGCAAGGCAATGATTATGGCTAGGAAAGAGAAGTCCGGGAAGTGGGTTGTTACAAAGCTTGAAATAAGACATTGTCATCCTATGTGGATTTCTAGAGGCAAGAAAGTCACCATTCCGGCTCCATCACAGGATGAGAAAGACAAGAAAATTCGTGAGCTATCTGCAGAGCTTTATCGGGCGAACCAACAGTTAGCAGAGTGCAGAAAAACACTTGAAATGGTTCTAAAAGAGGTAGACCAACACGCAGACTGCCTTACTAAAAGTGTTCAAAATATAGTTAAGAATGTAAAAGAGATTGAAGACAAGGATCGAGAGAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

230

Amino Acids

26.48

Weight (kDa)

8.18

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 62 - 150 7.7e-28 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022289)

Species Orthologous Gene IDs
malus_domestica MD05G1155300.v1.1 MD10G1149600.v1.1
pyrus_communis pycom05g14870 pycom10g12900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 688
AccI GTMKAC 1 cut(s) 600
AciI CCGC 1 cut(s) 14
AclWI GGATC 3 cut(s) 255, 268, 681
AcsI RAATTY 1 cut(s) 513
AcuI CTGAAG 1 cut(s) 30
AfiI CCNNNNNNNGG 2 cut(s) 149, 408
AgsI TTSAA 9 cut(s) 22, 50, 93, 116, 249, 428, 581, 635, 665
AjuI GAANNNNNNNTTGG 2 cut(s) 132, 164
AleI CACNNNNGTG 1 cut(s) 209
AluBI AGCT 3 cut(s) 424, 523, 535
AluI AGCT 3 cut(s) 424, 523, 535
AlwI GGATC 3 cut(s) 255, 268, 681
ApeKI GCWGC 1 cut(s) 366
ApoI RAATTY 1 cut(s) 513
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
Asp700I GAANNNNTTC 2 cut(s) 300, 585
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 341, 466
BamHI GGATCC 1 cut(s) 260
BauI CACGAG 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 353
BccI CCATC 4 cut(s) 249, 251, 272, 496
BciVI GTATCC 1 cut(s) 143
BcnI CCSGG 1 cut(s) 403
BfaI CTAG 5 cut(s) 86, 137, 357, 387, 459
BfmI CTRYAG 1 cut(s) 528
BfuI GTATCC 1 cut(s) 143
BisI GCNGC 1 cut(s) 367
BlsI GCNGC 1 cut(s) 368
Bme1390I CCNGG 1 cut(s) 403
BmiI GGNNCC 2 cut(s) 262, 486
BmrFI CCNGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 184
BpiI GAAGAC 1 cut(s) 672
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpuMI CCSGG 1 cut(s) 403
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 408
Bse118I RCCGGY 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 381
BseGI GGATG 3 cut(s) 61, 442, 502
BseLI CCNNNNNNNGG 2 cut(s) 149, 408
BseMI GCAATG 1 cut(s) 381
BseXI GCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 586
BsiSI CCGG 3 cut(s) 29, 402, 482
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 149, 408
BsmFI GGGAC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 260, 673
BspACI CCGC 1 cut(s) 14
BspLI GGNNCC 2 cut(s) 262, 486
BspMAI CTGCAG 1 cut(s) 532
BspPI GGATC 3 cut(s) 255, 268, 681
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 28
BssMI GATC 2 cut(s) 260, 673
BssSI CACGAG 1 cut(s) 207
Bst2BI CACGAG 1 cut(s) 207
Bst4CI ACNGT 2 cut(s) 272, 555
Bst6I CTCTTC 1 cut(s) 165
BstF5I GGATG 3 cut(s) 61, 442, 502
BstKTI GATC 2 cut(s) 263, 676
BstMBI GATC 2 cut(s) 260, 673
BstSCI CCNGG 1 cut(s) 401
BstSFI CTRYAG 1 cut(s) 528
BstV1I GCAGC 1 cut(s) 353
BstV2I GAAGAC 1 cut(s) 672
BstX2I RGATCY 1 cut(s) 260
BstXI CCANNNNNNTGG 1 cut(s) 211
BstYI RGATCY 1 cut(s) 260
BsuI GTATCC 1 cut(s) 143
BtsCI GGATG 3 cut(s) 61, 442, 502
Cfr10I RCCGGY 1 cut(s) 28
CspCI CAANNNNNGTGG 2 cut(s) 55, 90
CviJI RGCY 8 cut(s) 28, 176, 366, 386, 424, 485, 523, 535
CviKI_1 RGCY 8 cut(s) 28, 176, 366, 386, 424, 485, 523, 535
DpnI GATC 2 cut(s) 262, 675
DpnII GATC 2 cut(s) 260, 673
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 199
FaiI YATR 8 cut(s) 147, 192, 199, 201, 383, 449, 641, 688
FaqI GGGAC 1 cut(s) 115
FauNDI CATATG 1 cut(s) 199
FblI GTMKAC 1 cut(s) 600
Fnu4HI GCNGC 1 cut(s) 367
FokI GGATG 3 cut(s) 68, 429, 509
Fsp4HI GCNGC 1 cut(s) 367
FspBI CTAG 5 cut(s) 86, 137, 357, 387, 459
GluI GCNGC 1 cut(s) 367
HapII CCGG 3 cut(s) 29, 402, 482
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 4 cut(s) 5, 89, 164, 222
HpaII CCGG 3 cut(s) 29, 402, 482
HphI GGTGA 2 cut(s) 341, 466
Hpy166II GTNNAC 1 cut(s) 601
Hpy188I TCNGA 2 cut(s) 10, 169
Hpy188III TCNNGA 4 cut(s) 254, 459, 518, 677
Hpy8I GTNNAC 1 cut(s) 601
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 2 cut(s) 272, 555
HpyCH4V TGCA 4 cut(s) 197, 369, 530, 567
Kzo9I GATC 2 cut(s) 260, 673
LmnI GCTCC 1 cut(s) 490
LpnPI CCDG 4 cut(s) 42, 415, 479, 495
Lsp1109I GCAGC 1 cut(s) 353
LweI GCATC 1 cut(s) 184
MaeI CTAG 5 cut(s) 86, 137, 357, 387, 459
MaeIII GTNAC 5 cut(s) 233, 287, 305, 415, 472
MalI GATC 2 cut(s) 262, 675
MboI GATC 2 cut(s) 260, 673
MboII GAAGA 2 cut(s) 182, 677
MflI RGATCY 1 cut(s) 260
MluCI AATT 2 cut(s) 513, 683
MlyI GAGTC 2 cut(s) 14, 158
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 8 cut(s) 166, 318, 333, 339, 343, 355, 455, 589
Mph1103I ATGCAT 1 cut(s) 199
MroXI GAANNNNTTC 2 cut(s) 300, 585
MseI TTAA 1 cut(s) 645
MslI CAYNNNNRTG 1 cut(s) 209
MspI CCGG 3 cut(s) 29, 402, 482
MspR9I CCNGG 1 cut(s) 403
NciI CCSGG 1 cut(s) 403
NdeI CATATG 1 cut(s) 199
NdeII GATC 2 cut(s) 260, 673
NlaIV GGNNCC 2 cut(s) 262, 486
NmuCI GTSAC 1 cut(s) 472
NsiI ATGCAT 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 250
PdmI GAANNNNTTC 2 cut(s) 300, 585
PfeI GAWTC 2 cut(s) 89, 222
PflFI GACNNNGTC 1 cut(s) 438
PfoI TCCNGGA 1 cut(s) 401
PkrI GCNGC 1 cut(s) 368
PleI GAGTC 2 cut(s) 13, 158
PpsI GAGTC 2 cut(s) 13, 158
PsiI TTATAA 1 cut(s) 688
PspN4I GGNNCC 2 cut(s) 262, 486
PstI CTGCAG 1 cut(s) 532
PsuI RGATCY 1 cut(s) 260
PsyI GACNNNGTC 1 cut(s) 438
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 1 cut(s) 367
Sau3AI GATC 2 cut(s) 260, 673
SchI GAGTC 2 cut(s) 14, 158
ScrFI CCNGG 1 cut(s) 403
SetI ASST 7 cut(s) 145, 301, 344, 426, 525, 537, 600
SfaNI GCATC 1 cut(s) 184
SfcI CTRYAG 1 cut(s) 528
SmiMI CAYNNNNRTG 1 cut(s) 209
Sse9I AATT 2 cut(s) 513, 683
SsiI CCGC 1 cut(s) 14
SspMI CTAG 5 cut(s) 86, 137, 357, 387, 459
StyD4I CCNGG 1 cut(s) 401
TaaI ACNGT 2 cut(s) 272, 555
TaqI TCGA 2 cut(s) 344, 676
TasI AATT 2 cut(s) 513, 683
TfiI GAWTC 2 cut(s) 89, 222
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 1 cut(s) 366
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 1 cut(s) 215
Tth111I GACNNNGTC 1 cut(s) 438
XapI RAATTY 1 cut(s) 513
XbaI TCTAGA 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 600
XmnI GAANNNNTTC 2 cut(s) 300, 585
XspI CTAG 5 cut(s) 86, 137, 357, 387, 459
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.