pycom10g12900

FAR1 DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0022289
Physical Location & Seq
Forward (+)
16377762 .. 16379601
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12900.3

Sequence Viewer

Length: 837 bp
ATGTGGATCAAGAGAAACTGGGCGTTCTCAAATTTCTTCTCCCAGAGTTGGAGGCAAAAGGTCAGAAACGAGCGTGAATGCATTCAATGGCGGAAGGAAGCAACTTATGGAAGTGAGAATTACAGAATGGCTGCAGATCCTACTCCAATGGAGTCTGAAGCGGATTTGAACAGGATGGTCGTGGAGAGTCCAGTTGAAAGGGATATTCTGAACACTGAAAGTGGAGAAATGCTAGACTCAAACAATGGGACAGATTTGAAAGAAAATGAAGTAGATGGTATTCTAGAACCTTATGTTGGTATGGAGTTTGACTCTGAAGAGGCTGTCAAGGAATACTATGATGCATATGCCACGCGTGTGGGTTTTAGAATCCGTGTTGGTAATTTCCATCGTTCAAATCGTGATGGATCCATTAACAGTCGTAAGTTTCTTTGTAACAAAGAAGGTTTTCGTGACAATAAGCAATCAAAAAGGGGCGAAGTAAGGAGGCCGAGGGAGGAAACTAGGGAGGGCTGCAAGGCAATGATTATGGTGAGGAAAGAGAAGTCCGGGAAGTGGGTTGTTACAAAGCTTGAAACAAAACATTGTCATCCTATGTGGATTTCTAGAGGCAAGAGAGTCACCATTCCGGCTCCATCACAGGATGAGAAAGATAAGAAAATTCGTGAGCTATCTGCAGAGCTTTATCGAGCGAACCAACAGTTAGCAGAGTGCAGAAAAACACTTGAAATGGTCCTGAAAGATGTAGACCAACACGCAGACCACCTTACTATAAGTGTTCAAGACATAGTTAAGAATGTAAAACAGATTGAAGACAAGGATCGAGAGAATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

279

Amino Acids

32.69

Weight (kDa)

8.17

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022289)

Species Orthologous Gene IDs
malus_domestica MD05G1155300.v1.1 MD10G1149600.v1.1
pyrus_communis pycom05g14870 pycom10g12900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 835
AccI GTMKAC 1 cut(s) 747
AccII CGCG 1 cut(s) 355
AciI CCGC 2 cut(s) 91, 161
AclWI GGATC 5 cut(s) 14, 131, 402, 415, 828
AcsI RAATTY 3 cut(s) 31, 660, 829
AcuI CTGAAG 2 cut(s) 177, 336
AfiI CCNNNNNNNGG 3 cut(s) 48, 296, 555
AflIII ACRYGT 1 cut(s) 353
AgsI TTSAA 9 cut(s) 86, 169, 197, 259, 396, 575, 728, 782, 812
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AleI CACNNNNGTG 1 cut(s) 356
AluBI AGCT 3 cut(s) 571, 670, 682
AluI AGCT 3 cut(s) 571, 670, 682
AlwI GGATC 5 cut(s) 14, 131, 402, 415, 828
AoxI GGCC 1 cut(s) 488
ApeKI GCWGC 2 cut(s) 131, 513
ApoI RAATTY 3 cut(s) 31, 660, 829
Asp700I GAANNNNTTC 2 cut(s) 81, 447
AspS9I GGNCC 1 cut(s) 733
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 2 cut(s) 544, 613
AvaII GGWCC 1 cut(s) 733
BamHI GGATCC 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 819
BbvI GCAGC 2 cut(s) 118, 500
BccI CCATC 5 cut(s) 169, 269, 396, 398, 643
BcnI CCSGG 1 cut(s) 550
BfaI CTAG 4 cut(s) 233, 284, 504, 606
BfmI CTRYAG 2 cut(s) 132, 675
BisI GCNGC 2 cut(s) 132, 514
BlsI GCNGC 2 cut(s) 133, 515
Bme1390I CCNGG 1 cut(s) 550
Bme18I GGWCC 1 cut(s) 733
BmgT120I GGNCC 1 cut(s) 733
BmiI GGNNCC 2 cut(s) 409, 633
BmrFI CCNGG 1 cut(s) 550
BmrI ACTGGG 1 cut(s) 28
BmsI GCATC 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 819
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpuMI CCSGG 1 cut(s) 550
BsaJI CCNNGG 1 cut(s) 491
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 296, 555
Bse1I ACTGG 2 cut(s) 23, 191
Bse3DI GCAATG 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 3 cut(s) 180, 589, 649
BseLI CCNNNNNNNGG 3 cut(s) 48, 296, 555
BseMI GCAATG 1 cut(s) 528
BseNI ACTGG 2 cut(s) 23, 191
BseXI GCAGC 2 cut(s) 118, 500
BsgI GTGCAG 1 cut(s) 733
Bsh1236I CGCG 1 cut(s) 355
BshFI GGCC 1 cut(s) 490
BsiSI CCGG 2 cut(s) 549, 629
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 3 cut(s) 48, 296, 555
BsmFI GGGAC 1 cut(s) 262
BsmI GAATGC 2 cut(s) 81, 83
BsnI GGCC 1 cut(s) 490
Bsp143I GATC 4 cut(s) 6, 136, 407, 820
BspACI CCGC 2 cut(s) 91, 161
BspANI GGCC 1 cut(s) 490
BspFNI CGCG 1 cut(s) 355
BspLI GGNNCC 2 cut(s) 409, 633
BspMAI CTGCAG 2 cut(s) 136, 679
BspPI GGATC 5 cut(s) 14, 131, 402, 415, 828
BsrDI GCAATG 1 cut(s) 528
BsrI ACTGG 2 cut(s) 23, 191
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 4 cut(s) 6, 136, 407, 820
Bst4CI ACNGT 2 cut(s) 419, 702
Bst6I CTCTTC 1 cut(s) 312
BstF5I GGATG 3 cut(s) 180, 589, 649
BstFNI CGCG 1 cut(s) 355
BstKTI GATC 4 cut(s) 9, 139, 410, 823
BstMBI GATC 4 cut(s) 6, 136, 407, 820
BstSCI CCNGG 1 cut(s) 548
BstSFI CTRYAG 2 cut(s) 132, 675
BstUI CGCG 1 cut(s) 355
BstV1I GCAGC 2 cut(s) 118, 500
BstV2I GAAGAC 1 cut(s) 819
BstX2I RGATCY 2 cut(s) 136, 407
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 2 cut(s) 136, 407
BsuRI GGCC 1 cut(s) 490
BtsCI GGATG 3 cut(s) 180, 589, 649
BtsIMutI CAGTG 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 733
CviJI RGCY 8 cut(s) 131, 323, 490, 513, 571, 632, 670, 682
CviKI_1 RGCY 8 cut(s) 131, 323, 490, 513, 571, 632, 670, 682
DpnI GATC 4 cut(s) 8, 138, 409, 822
DpnII GATC 4 cut(s) 6, 136, 407, 820
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
EciI GGCGGA 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 733
Eco57I CTGAAG 2 cut(s) 177, 336
EcoT22I ATGCAT 2 cut(s) 83, 346
FaqI GGGAC 1 cut(s) 262
FauNDI CATATG 1 cut(s) 346
FblI GTMKAC 1 cut(s) 747
Fnu4HI GCNGC 2 cut(s) 132, 514
FokI GGATG 3 cut(s) 187, 576, 656
Fsp4HI GCNGC 2 cut(s) 132, 514
FspBI CTAG 4 cut(s) 233, 284, 504, 606
GluI GCNGC 2 cut(s) 132, 514
HaeIII GGCC 1 cut(s) 490
HapII CCGG 2 cut(s) 549, 629
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 6 cut(s) 152, 187, 236, 311, 369, 618
HpaII CCGG 2 cut(s) 549, 629
HphI GGTGA 2 cut(s) 544, 613
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 4 cut(s) 65, 157, 210, 316
Hpy188III TCNNGA 9 cut(s) 10, 284, 401, 452, 606, 665, 736, 782, 824
Hpy8I GTNNAC 1 cut(s) 748
HpyAV CCTTC 2 cut(s) 88, 437
HpyCH4III ACNGT 2 cut(s) 419, 702
HpyCH4V TGCA 6 cut(s) 81, 134, 344, 516, 677, 714
Kzo9I GATC 4 cut(s) 6, 136, 407, 820
LmnI GCTCC 1 cut(s) 637
LpnPI CCDG 8 cut(s) 4, 56, 157, 204, 562, 626, 642, 749
Lsp1109I GCAGC 2 cut(s) 118, 500
LweI GCATC 1 cut(s) 331
MaeI CTAG 4 cut(s) 233, 284, 504, 606
MaeIII GTNAC 4 cut(s) 434, 452, 562, 619
MalI GATC 4 cut(s) 8, 138, 409, 822
MboI GATC 4 cut(s) 6, 136, 407, 820
MboII GAAGA 3 cut(s) 28, 329, 824
MflI RGATCY 2 cut(s) 136, 407
MluCI AATT 5 cut(s) 31, 118, 382, 660, 829
MluI ACGCGT 1 cut(s) 353
MlyI GAGTC 5 cut(s) 161, 196, 230, 305, 627
MmeI TCCRAC 1 cut(s) 29
MnlI CCTC 8 cut(s) 45, 313, 480, 486, 490, 502, 528, 602
Mph1103I ATGCAT 2 cut(s) 83, 346
MroXI GAANNNNTTC 2 cut(s) 81, 447
MseI TTAA 2 cut(s) 414, 792
MslI CAYNNNNRTG 1 cut(s) 356
MspI CCGG 2 cut(s) 549, 629
MspR9I CCNGG 1 cut(s) 550
Mva1269I GAATGC 2 cut(s) 81, 83
MvnI CGCG 1 cut(s) 355
NciI CCSGG 1 cut(s) 550
NdeI CATATG 1 cut(s) 346
NdeII GATC 4 cut(s) 6, 136, 407, 820
NlaIV GGNNCC 2 cut(s) 409, 633
NmeAIII GCCGAG 1 cut(s) 516
NmuCI GTSAC 2 cut(s) 452, 619
NsiI ATGCAT 2 cut(s) 83, 346
OliI CACNNNNGTG 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 397
PctI GAATGC 2 cut(s) 81, 83
PdmI GAANNNNTTC 2 cut(s) 81, 447
PfeI GAWTC 1 cut(s) 369
PfoI TCCNGGA 1 cut(s) 548
PkrI GCNGC 2 cut(s) 133, 515
PleI GAGTC 5 cut(s) 160, 195, 230, 305, 626
PpsI GAGTC 5 cut(s) 160, 195, 230, 305, 626
PsiI TTATAA 1 cut(s) 835
PspN4I GGNNCC 2 cut(s) 409, 633
PspPI GGNCC 1 cut(s) 733
PstI CTGCAG 2 cut(s) 136, 679
PsuI RGATCY 2 cut(s) 136, 407
RseI CAYNNNNRTG 1 cut(s) 356
SaqAI TTAA 2 cut(s) 414, 792
SatI GCNGC 2 cut(s) 132, 514
Sau3AI GATC 4 cut(s) 6, 136, 407, 820
Sau96I GGNCC 1 cut(s) 733
SchI GAGTC 5 cut(s) 161, 196, 230, 305, 627
ScrFI CCNGG 1 cut(s) 550
SetI ASST 7 cut(s) 63, 292, 448, 573, 672, 684, 768
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 2 cut(s) 132, 675
SinI GGWCC 1 cut(s) 733
SmiMI CAYNNNNRTG 1 cut(s) 356
Sse9I AATT 5 cut(s) 31, 118, 382, 660, 829
SsiI CCGC 2 cut(s) 91, 161
SspMI CTAG 4 cut(s) 233, 284, 504, 606
StyD4I CCNGG 1 cut(s) 548
TaaI ACNGT 2 cut(s) 419, 702
TaqI TCGA 2 cut(s) 688, 823
TasI AATT 5 cut(s) 31, 118, 382, 660, 829
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 2 cut(s) 414, 792
Tru9I TTAA 2 cut(s) 414, 792
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 2 cut(s) 452, 619
TseI GCWGC 2 cut(s) 131, 513
Tsp45I GTSAC 2 cut(s) 452, 619
TspDTI ATGAA 1 cut(s) 282
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 733
XapI RAATTY 3 cut(s) 31, 660, 829
XbaI TCTAGA 2 cut(s) 283, 605
XmiI GTMKAC 1 cut(s) 747
XmnI GAANNNNTTC 2 cut(s) 81, 447
XspI CTAG 4 cut(s) 233, 284, 504, 606
Zsp2I ATGCAT 2 cut(s) 83, 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.