MD10G1149600.v1.1

FAR1 DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0022289
Physical Location & Seq
Forward (+)
23682308 .. 23684740
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1149600.v1.1.491

Sequence Viewer

Length: 738 bp
ATGCAATCACTGGCGGAAGGAAGCAAAATGGCTGCAGATCCTACTCCGATGGAATCTGAAGCGGATTTGAACAGGCTGGTCGTGGAGAGTCCAGTTGAAAGGGATATTCCGAACGCTGAAAGTGGAGAAAGGCTAGACTCAAATAATGTGACGGACTTGAATGAAAATGAAGTAGATGGTATTCTAGAACCTTATGTTGGTATGGAGTTTGACTCTGAAGAGGCTATCAAGGAATACTATGATGCATATGCCACGCGTGTGGGTTTTAGAATCCGTGTTGGTAATTTCCATCGTTCAAATCGTGATGGATCCATTAACAGTCGTAAGTTTCTTTGTAACAAAGAAGGTTTTCGTGACAATAAGCAGTCAAAAAGGGGCGAAGTAAGGAGGCCGAGGGAGGAAACTAGGGAGGGCTGCAAGGCAATGATTATGGCAAGGAAAGAGAAGTCCGGGAAGTGGGTTGTTACAAAGCTTGAAACAAAACATTGTCATCCTATGTGGATTTCTAGAGGCAAGAGAGTCACCATTCCGGCTCCATCACAGGACGAGAAAGATAAGAAAATTCGTGAGCTATCTGCAGAGCTTTATCGAGCGAACCAACAGTTAGCAGGGTGTAGAAAAACACTTGAAATGGTCCTGAAAGAGGTAGACCAACATGCAGACCGCCTTACTAAAAGTGTTCAAGACATAGTTAAGAATGTAAAACAGATTGAAGACAAGGATAGAGAGAATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

246

Amino Acids

28.17

Weight (kDa)

6.93

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 78 - 166 8.7e-29 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022289)

Species Orthologous Gene IDs
malus_domestica MD05G1155300.v1.1 MD10G1149600.v1.1
pyrus_communis pycom05g14870 pycom10g12900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 736
AccI GTMKAC 1 cut(s) 648
AccII CGCG 1 cut(s) 256
AciI CCGC 3 cut(s) 14, 62, 664
AclWI GGATC 3 cut(s) 32, 303, 316
AcsI RAATTY 2 cut(s) 561, 730
AcuI CTGAAG 2 cut(s) 78, 237
AfiI CCNNNNNNNGG 3 cut(s) 197, 456, 643
AflIII ACRYGT 1 cut(s) 254
AgsI TTSAA 8 cut(s) 70, 98, 160, 297, 476, 629, 683, 713
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AleI CACNNNNGTG 1 cut(s) 257
AluBI AGCT 3 cut(s) 472, 571, 583
AluI AGCT 3 cut(s) 472, 571, 583
AlwI GGATC 3 cut(s) 32, 303, 316
AoxI GGCC 1 cut(s) 389
ApeKI GCWGC 2 cut(s) 32, 414
ApoI RAATTY 2 cut(s) 561, 730
Asp700I GAANNNNTTC 1 cut(s) 348
AspS9I GGNCC 1 cut(s) 634
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 1 cut(s) 514
AvaII GGWCC 1 cut(s) 634
BamHI GGATCC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 720
BbvI GCAGC 2 cut(s) 19, 401
BccI CCATC 5 cut(s) 43, 170, 297, 299, 544
BcnI CCSGG 1 cut(s) 451
BfaI CTAG 4 cut(s) 134, 185, 405, 507
BfmI CTRYAG 2 cut(s) 33, 576
BisI GCNGC 2 cut(s) 33, 415
BlsI GCNGC 2 cut(s) 34, 416
Bme1390I CCNGG 1 cut(s) 451
Bme18I GGWCC 1 cut(s) 634
BmgT120I GGNCC 1 cut(s) 634
BmiI GGNNCC 2 cut(s) 310, 534
BmrFI CCNGG 1 cut(s) 451
BmsI GCATC 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 720
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpuMI CCSGG 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 392
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 456, 643
Bse1I ACTGG 2 cut(s) 15, 92
Bse3DI GCAATG 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 3 cut(s) 197, 456, 643
BseMI GCAATG 1 cut(s) 429
BseNI ACTGG 2 cut(s) 15, 92
BseXI GCAGC 2 cut(s) 19, 401
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 1 cut(s) 391
BsiSI CCGG 2 cut(s) 450, 530
BslI CCNNNNNNNGG 3 cut(s) 197, 456, 643
BsnI GGCC 1 cut(s) 391
Bsp143I GATC 2 cut(s) 37, 308
BspACI CCGC 3 cut(s) 14, 62, 664
BspANI GGCC 1 cut(s) 391
BspFNI CGCG 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 310, 534
BspMAI CTGCAG 2 cut(s) 37, 580
BspPI GGATC 3 cut(s) 32, 303, 316
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 2 cut(s) 15, 92
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 2 cut(s) 37, 308
Bst4CI ACNGT 2 cut(s) 320, 603
Bst6I CTCTTC 1 cut(s) 213
BstENI CCTNNNNNAGG 1 cut(s) 641
BstF5I GGATG 1 cut(s) 490
BstFNI CGCG 1 cut(s) 256
BstKTI GATC 2 cut(s) 40, 311
BstMBI GATC 2 cut(s) 37, 308
BstNSI RCATGY 1 cut(s) 659
BstSCI CCNGG 1 cut(s) 449
BstSFI CTRYAG 2 cut(s) 33, 576
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 2 cut(s) 19, 401
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 2 cut(s) 37, 308
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 2 cut(s) 37, 308
BsuRI GGCC 1 cut(s) 391
BtsCI GGATG 1 cut(s) 490
BtsIMutI CAGTG 1 cut(s) 8
Cfr13I GGNCC 1 cut(s) 634
CviAII CATG 1 cut(s) 656
DpnI GATC 2 cut(s) 39, 310
DpnII GATC 2 cut(s) 37, 308
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
EciI GGCGGA 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 634
Eco57I CTGAAG 2 cut(s) 78, 237
EcoNI CCTNNNNNAGG 1 cut(s) 641
EcoT22I ATGCAT 1 cut(s) 247
FaeI CATG 1 cut(s) 659
FatI CATG 1 cut(s) 655
FauNDI CATATG 1 cut(s) 247
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 2 cut(s) 33, 415
FokI GGATG 1 cut(s) 477
Fsp4HI GCNGC 2 cut(s) 33, 415
FspBI CTAG 4 cut(s) 134, 185, 405, 507
GluI GCNGC 2 cut(s) 33, 415
HaeIII GGCC 1 cut(s) 391
HapII CCGG 2 cut(s) 450, 530
Hin1II CATG 1 cut(s) 659
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 6 cut(s) 53, 88, 137, 212, 270, 519
HpaII CCGG 2 cut(s) 450, 530
HphI GGTGA 1 cut(s) 514
Hpy166II GTNNAC 1 cut(s) 649
Hpy188I TCNGA 4 cut(s) 48, 58, 111, 217
Hpy188III TCNNGA 7 cut(s) 185, 302, 353, 507, 566, 637, 683
Hpy8I GTNNAC 1 cut(s) 649
HpyAV CCTTC 2 cut(s) 11, 338
HpyCH4III ACNGT 2 cut(s) 320, 603
HpyCH4V TGCA 6 cut(s) 4, 35, 245, 417, 578, 659
Hsp92II CATG 1 cut(s) 659
Kzo9I GATC 2 cut(s) 37, 308
LmnI GCTCC 1 cut(s) 538
LpnPI CCDG 8 cut(s) 58, 62, 105, 463, 527, 543, 594, 650
Lsp1109I GCAGC 2 cut(s) 19, 401
LweI GCATC 1 cut(s) 232
MaeI CTAG 4 cut(s) 134, 185, 405, 507
MaeIII GTNAC 5 cut(s) 148, 335, 353, 463, 520
MalI GATC 2 cut(s) 39, 310
MboI GATC 2 cut(s) 37, 308
MboII GAAGA 2 cut(s) 230, 725
MflI RGATCY 2 cut(s) 37, 308
MluCI AATT 3 cut(s) 283, 561, 730
MluI ACGCGT 1 cut(s) 254
MlyI GAGTC 4 cut(s) 97, 131, 206, 528
MnlI CCTC 7 cut(s) 214, 381, 387, 391, 403, 503, 637
Mph1103I ATGCAT 1 cut(s) 247
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 2 cut(s) 315, 693
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 2 cut(s) 450, 530
MspR9I CCNGG 1 cut(s) 451
MvnI CGCG 1 cut(s) 256
NciI CCSGG 1 cut(s) 451
NdeI CATATG 1 cut(s) 247
NdeII GATC 2 cut(s) 37, 308
NlaIII CATG 1 cut(s) 659
NlaIV GGNNCC 2 cut(s) 310, 534
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 3 cut(s) 148, 353, 520
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 1 cut(s) 659
OliI CACNNNNGTG 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 2 cut(s) 53, 270
PfoI TCCNGGA 1 cut(s) 449
PkrI GCNGC 2 cut(s) 34, 416
PleI GAGTC 4 cut(s) 96, 131, 206, 527
PpsI GAGTC 4 cut(s) 96, 131, 206, 527
PsiI TTATAA 1 cut(s) 736
PspN4I GGNNCC 2 cut(s) 310, 534
PspPI GGNCC 1 cut(s) 634
PstI CTGCAG 2 cut(s) 37, 580
PsuI RGATCY 2 cut(s) 37, 308
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 2 cut(s) 315, 693
SatI GCNGC 2 cut(s) 33, 415
Sau3AI GATC 2 cut(s) 37, 308
Sau96I GGNCC 1 cut(s) 634
SchI GAGTC 4 cut(s) 97, 131, 206, 528
ScrFI CCNGG 1 cut(s) 451
SetI ASST 6 cut(s) 193, 349, 474, 573, 585, 648
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 2 cut(s) 33, 576
SinI GGWCC 1 cut(s) 634
SmiMI CAYNNNNRTG 1 cut(s) 257
Sse9I AATT 3 cut(s) 283, 561, 730
SsiI CCGC 3 cut(s) 14, 62, 664
SspMI CTAG 4 cut(s) 134, 185, 405, 507
StyD4I CCNGG 1 cut(s) 449
TaaI ACNGT 2 cut(s) 320, 603
TaqI TCGA 1 cut(s) 589
TasI AATT 3 cut(s) 283, 561, 730
TfiI GAWTC 2 cut(s) 53, 270
Tru1I TTAA 2 cut(s) 315, 693
Tru9I TTAA 2 cut(s) 315, 693
TscAI CASTG 1 cut(s) 15
TseFI GTSAC 3 cut(s) 148, 353, 520
TseI GCWGC 2 cut(s) 32, 414
Tsp45I GTSAC 3 cut(s) 148, 353, 520
TspDTI ATGAA 2 cut(s) 177, 183
TspGWI ACGGA 2 cut(s) 167, 263
TspRI CASTG 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 634
XagI CCTNNNNNAGG 1 cut(s) 641
XapI RAATTY 2 cut(s) 561, 730
XbaI TCTAGA 2 cut(s) 184, 506
XceI RCATGY 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 648
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 4 cut(s) 134, 185, 405, 507
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.