MD05G1187900.v1.1

xyloglucan galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014475
Physical Location & Seq
Forward (+)
31599891 .. 31602346
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1187900.v1.1.491

Sequence Viewer

Length: 1764 bp
ATGAATCCAATGAGACGCCGCTCTCCGACGAGTGTACCTGTGGAGCCAATGGAGAAGGGGACAGGAAAGAATCAGAATACAAGGATTTGTTTCTTGGCCTCTTTGTCTGTGTTCTTCTGGATGTTGTTGTTGTACTTCCATTTCGTTGTGCTGGGAGGTAGTACCGTGAATGAATCTTTCAATTTACAAGCTGGTCGTGTTTACTCCAAAGTGAAGCCTACCCTTGTAATTAATGATCTTCAGAGGGACAGCCAGAGCACTGAATCAACACCTATCCCTGTAGCCCCTGCGAAAAACAACCAGATCAATGAAGCGGAGAAGTATCCATTTATGAGAGCATTGAGAACTGTCGAAAACAAGAGTGATCCATGTGGTGGGAAGTATATTTATGTGCATGATCTTCCCTCGAGGTTTAATGAGGATATGTTAAAGGAATGTAGGAGTTTAAGCCGTTGGACAAATATGTGCAAGTTCACAACTAATGCTGGGCTTGGGCCTCCACTTGAGAATGCAGAGGGTGTGTTTGGGGATACGGGTTGGTATGCCACGAATCAGTTCGCTGTGGATGTCATATTTAACAATCGGATGAAGCAATATGAGTGTTTGACAAATGATTCATCTCTTGCTGCTGCTATTTTTGTTCCCTTTTATGCTGGGTTTGACATTGCCCGGTACCTTTGGGGTTTCAACATATCAAGGAGGGATGCTGCCTCACTCGATCTGGTTGATTGGCTTACGAAGAGGCCAGAATGGAGCATTATGGGTGGCAAAGACCACTTTCTTGTTGCAGGAAGGATAACTTGGGATTTCAGGAGACTTTCAGAGGAAGAATCGGATTGGGGTAACAAGCTTCTCTTTCTGCCAGCTGCTAAGAATATGTCTATGCTTGTGGTAGAATCAAGCCCGTGGAATGCTAATGATTTTGGTATTCCATATCCCACATATTTCCATCCAGCAAAAGATGCTGATGTGTTCATTTGGCAGGAACGAATGAGAAGATTAAAGCGTGAGTTCCTTTTCTCTTTTGCTGGTGCCCCCCGTCCTGACAATCCCAAGTCAATCAGGGGGAAGATCATTGATCAGTGCCGGAGTTCAAAGGTTGGTAAGCTGTTGGAATGTGATTTTGGGGAAAGCAAGTGTCATTCTCCAAGCAGTATAATGCATATGTTTCAGAGATCTCTTTTCTGCCTGCAACCGCAAGGCGATTCATACACACGAAGATCTGCTTTTGATTCAATGTTGGCTGGTTGCATACCTGTCTTCTTCCATCCTGGGTCGGCATACACACAATATACTTGGCATTTACCAAAAGATTATACAAGGTATTCAGTGTTCATCCCAGAGGACGATATTCGCAAGAGGAACGTTAGCATAGAGGAAACACTTAGTCGAATTACTCCTGAGCAGGTGAAGAAAATGAGGGAGGAGGTCATAAGCCTTATTCCAAGTCTGATATACGCCGATCCCCGCTCAAAGTTGGGGATTTTTAGAGATGCCTTTGATGTTTCTGTACAGGCCGTCATTGACAAAGTTACAAAGTTGAGGAAGGACATAATCGAGGGTCGTACAGATGATAACTTCATCGAAGAAAACAGTTGGAAGTATGCTTTGTTGGAGGAGGGACAACGTGAGGTAGGAGCTCACGAATGGGATCCTTTCTTCTCGAAGCCAAAGGATGGTAATGGTGATTCTGTTGATACATCTGCCGAAGCTGCGAAAAACTCGTGGCATAATGAACAACGACATCAATCATCGGGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000271 GO:0000902 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0005996 GO:0006004 GO:0006073 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009826 GO:0009863 GO:0009969 GO:0009987 GO:0010033 GO:0010256 GO:0010383 GO:0010410 GO:0010411 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0016051 GO:0016740 GO:0016757 GO:0019318 GO:0019319 GO:0023052 GO:0030173 GO:0031090 GO:0031224 GO:0031228 GO:0031300 GO:0031301 GO:0031410 GO:0031982 GO:0031984 GO:0032502 GO:0032870 GO:0032989 GO:0033692 GO:0034637 GO:0034645 GO:0035690 GO:0040007 GO:0042221 GO:0042353 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046364 GO:0046677 GO:0048589 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060560 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071554 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:1901576 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

588

Amino Acids

67.25

Weight (kDa)

7.93

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 124 - 461 2.3e-87 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014475)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20370
fragaria_vesca FvH4_2g20390
malus_domestica MD05G1187900.v1.1 MD10G1175500.v1.1
pyrus_communis pycom05g17260 pycom10g15130
rosa_chinensis RchiOBHm_Chr6g0286491
rosa_laevigata RLG00000012565
rosa_multiflora Rmu_sc0004816.1_g000011
rosa_roxburghii Rroxscaffold_7G00182280
rosa_rugosa Rorug06G0180200 Rorug06G0180300
rosa_samantha Rh6AG292600 Rh6BG296100 Rh6CG296900 Rh6DG288600
rosa_wichuraiana Rw6G025220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1396
AbsI CCTCGAGG 1 cut(s) 406
Acc36I ACCTGC 1 cut(s) 1396
Acc65I GGTACC 1 cut(s) 672
AccB1I GGYRCC 2 cut(s) 672, 1031
AccB7I CCANNNNNTGG 2 cut(s) 374, 1676
AccBSI CCGCTC 2 cut(s) 21, 1470
AciI CCGC 4 cut(s) 19, 314, 1196, 1468
AclI AACGTT 1 cut(s) 1365
AclWI GGATC 4 cut(s) 359, 1457, 1646, 1659
AcuI CTGAAG 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 6 cut(s) 36, 134, 163, 674, 1512, 1567
AfiI CCNNNNNNNGG 2 cut(s) 374, 1676
AgsI TTSAA 4 cut(s) 181, 688, 1095, 1236
AjnI CCWGG 1 cut(s) 1270
AjuI GAANNNNNNNTTGG 4 cut(s) 784, 816, 1107, 1139
AluBI AGCT 6 cut(s) 191, 850, 866, 1108, 1640, 1712
AluI AGCT 6 cut(s) 191, 850, 866, 1108, 1640, 1712
Alw21I GWGCWC 2 cut(s) 260, 1642
Alw26I GTCTC 2 cut(s) 7, 808
AlwI GGATC 4 cut(s) 359, 1457, 1646, 1659
Ama87I CYCGRG 1 cut(s) 406
AoxI GGCC 4 cut(s) 96, 494, 743, 1515
ApeKI GCWGC 5 cut(s) 626, 629, 707, 866, 1712
AseI ATTAAT 1 cut(s) 231
Asp700I GAANNNNTTC 1 cut(s) 554
Asp718I GGTACC 1 cut(s) 672
AspS9I GGNCC 1 cut(s) 494
AsuC2I CCSGG 1 cut(s) 670
AsuHPI GGTGA 2 cut(s) 1420, 1697
AvaI CYCGRG 1 cut(s) 406
BaeGI GKGCMC 1 cut(s) 1036
BaeI ACNNNNGTAYC 2 cut(s) 18, 51
BamHI GGATCC 1 cut(s) 1651
BanI GGYRCC 2 cut(s) 672, 1031
BanII GRGCYC 1 cut(s) 1642
BauI CACGAG 1 cut(s) 1723
BbsI GAAGAC 1 cut(s) 1252
Bbv12I GWGCWC 2 cut(s) 260, 1642
BbvI GCAGC 5 cut(s) 613, 616, 694, 853, 1699
BccI CCATC 3 cut(s) 957, 1275, 1670
BceAI ACGGC 2 cut(s) 435, 1502
BciT130I CCWGG 1 cut(s) 1272
BciVI GTATCC 2 cut(s) 333, 523
BclI TGATCA 1 cut(s) 1078
BcnI CCSGG 1 cut(s) 670
BcoDI GTCTC 2 cut(s) 7, 808
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 1396
BfuI GTATCC 2 cut(s) 333, 523
BglII AGATCT 2 cut(s) 1175, 1220
BisI GCNGC 6 cut(s) 19, 627, 630, 708, 867, 1713
BlsI GCNGC 6 cut(s) 20, 628, 631, 709, 868, 1714
Bme1390I CCNGG 2 cut(s) 670, 1272
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 4 cut(s) 45, 674, 1033, 1653
BmrFI CCNGG 2 cut(s) 670, 1272
BmsI GCATC 3 cut(s) 694, 952, 1483
BpiI GAAGAC 1 cut(s) 1252
Bpu10I CCTNAGC 1 cut(s) 1401
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 670
BsaHI GRCGYC 1 cut(s) 16
BsaJI CCNNGG 2 cut(s) 905, 1271
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 1676
Bse3DI GCAATG 1 cut(s) 663
BseBI CCWGG 1 cut(s) 1272
BseDI CCNNGG 2 cut(s) 905, 1271
BseGI GGATG 8 cut(s) 126, 571, 591, 709, 949, 1267, 1335, 1681
BseLI CCNNNNNNNGG 2 cut(s) 374, 1676
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 1 cut(s) 1392
BseRI GAGGAG 2 cut(s) 1439, 1631
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 5 cut(s) 613, 616, 694, 853, 1699
BseYI CCCAGC 3 cut(s) 151, 485, 653
BshFI GGCC 4 cut(s) 98, 496, 745, 1517
BshNI GGYRCC 2 cut(s) 672, 1031
BsiHKAI GWGCWC 2 cut(s) 260, 1642
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 2 cut(s) 670, 1087
BslFI GGGAC 3 cut(s) 73, 260, 1635
BslI CCNNNNNNNGG 2 cut(s) 374, 1676
BsmAI GTCTC 2 cut(s) 7, 808
BsmBI CGTCTC 1 cut(s) 7
BsmFI GGGAC 3 cut(s) 73, 260, 1635
BsmI GAATGC 2 cut(s) 514, 916
BsnI GGCC 4 cut(s) 98, 496, 745, 1517
BsoBI CYCGRG 1 cut(s) 406
Bsp1286I GDGCHC 3 cut(s) 260, 1036, 1642
Bsp1407I TGTACA 1 cut(s) 1510
BspACI CCGC 4 cut(s) 19, 314, 1196, 1468
BspANI GGCC 4 cut(s) 98, 496, 745, 1517
BspCNI CTCAG 1 cut(s) 1393
BspLI GGNNCC 4 cut(s) 45, 674, 1033, 1653
BspMI ACCTGC 1 cut(s) 1396
BspPI GGATC 4 cut(s) 359, 1457, 1646, 1659
BspT107I GGYRCC 2 cut(s) 672, 1031
BsrBI CCGCTC 2 cut(s) 21, 1470
BsrDI GCAATG 1 cut(s) 663
BsrGI TGTACA 1 cut(s) 1510
BssECI CCNNGG 2 cut(s) 905, 1271
BssNI GRCGYC 1 cut(s) 16
BssSI CACGAG 1 cut(s) 1723
Bst2BI CACGAG 1 cut(s) 1723
Bst2UI CCWGG 1 cut(s) 1272
Bst4CI ACNGT 3 cut(s) 166, 349, 1595
Bst6I CTCTTC 1 cut(s) 734
BstACI GRCGYC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 962
BstAUI TGTACA 1 cut(s) 1510
BstC8I GCNNGC 2 cut(s) 864, 1190
BstDEI CTNAG 3 cut(s) 870, 1385, 1401
BstDSI CCRYGG 1 cut(s) 905
BstF5I GGATG 8 cut(s) 126, 571, 591, 709, 949, 1267, 1335, 1681
BstMAI GTCTC 2 cut(s) 7, 808
BstMWI GCNNNNNNNGC 2 cut(s) 962, 1712
BstNI CCWGG 1 cut(s) 1272
BstSCI CCNGG 2 cut(s) 668, 1270
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 1036
BstV1I GCAGC 5 cut(s) 613, 616, 694, 853, 1699
BstV2I GAAGAC 1 cut(s) 1252
BstX2I RGATCY 3 cut(s) 1175, 1220, 1651
BstYI RGATCY 3 cut(s) 1175, 1220, 1651
BsuI GTATCC 2 cut(s) 333, 523
BsuRI GGCC 4 cut(s) 98, 496, 745, 1517
BtgI CCRYGG 1 cut(s) 905
BtsCI GGATG 8 cut(s) 126, 571, 591, 709, 949, 1267, 1335, 1681
BtsIMutI CAGTG 3 cut(s) 258, 1088, 1335
BveI ACCTGC 1 cut(s) 1396
Cac8I GCNNGC 2 cut(s) 864, 1190
Cfr13I GGNCC 1 cut(s) 494
CseI GACGC 1 cut(s) 24
Csp6I GTAC 6 cut(s) 35, 133, 162, 673, 1511, 1566
CviAII CATG 2 cut(s) 369, 395
CviQI GTAC 6 cut(s) 35, 133, 162, 673, 1511, 1566
DdeI CTNAG 3 cut(s) 870, 1385, 1401
Eam1104I CTCTTC 1 cut(s) 734
EarI CTCTTC 1 cut(s) 734
Ecl136II GAGCTC 1 cut(s) 1640
Eco24I GRGCYC 1 cut(s) 1642
Eco53kI GAGCTC 1 cut(s) 1640
Eco57I CTGAAG 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 1640
EcoRII CCWGG 1 cut(s) 1270
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 1 cut(s) 1642
Esp3I CGTCTC 1 cut(s) 7
FaeI CATG 2 cut(s) 372, 398
FalI AAGNNNNNCTT 6 cut(s) 784, 816, 839, 871, 1210, 1242
FaqI GGGAC 3 cut(s) 73, 260, 1635
FatI CATG 2 cut(s) 368, 394
FauI CCCGC 1 cut(s) 1475
FauNDI CATATG 1 cut(s) 1164
FbaI TGATCA 1 cut(s) 1078
Fnu4HI GCNGC 6 cut(s) 19, 627, 630, 708, 867, 1713
FokI GGATG 8 cut(s) 133, 578, 598, 716, 936, 1254, 1322, 1688
FriOI GRGCYC 1 cut(s) 1642
Fsp4HI GCNGC 6 cut(s) 19, 627, 630, 708, 867, 1713
GluI GCNGC 6 cut(s) 19, 627, 630, 708, 867, 1713
GsaI CCCAGC 3 cut(s) 155, 489, 657
HaeIII GGCC 4 cut(s) 98, 496, 745, 1517
HapII CCGG 2 cut(s) 670, 1087
HgaI GACGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 2 cut(s) 372, 398
HindIII AAGCTT 1 cut(s) 848
HpaII CCGG 2 cut(s) 670, 1087
HphI GGTGA 2 cut(s) 1420, 1697
Hpy166II GTNNAC 3 cut(s) 35, 202, 474
Hpy188I TCNGA 8 cut(s) 27, 75, 243, 585, 823, 835, 1173, 1452
Hpy188III TCNNGA 6 cut(s) 118, 811, 1043, 1400, 1643, 1663
Hpy8I GTNNAC 3 cut(s) 35, 202, 474
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 3 cut(s) 49, 786, 1540
HpyCH4III ACNGT 3 cut(s) 166, 349, 1595
HpyCH4IV ACGT 2 cut(s) 1365, 1627
HpyCH4V TGCA 7 cut(s) 394, 468, 512, 788, 1162, 1192, 1251
HpyF10VI GCNNNNNNNGC 2 cut(s) 962, 1712
HpyF3I CTNAG 3 cut(s) 870, 1385, 1401
HpySE526I ACGT 2 cut(s) 1365, 1627
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 2 cut(s) 372, 398
KpnI GGTACC 1 cut(s) 676
Ksp22I TGATCA 1 cut(s) 1078
LmnI GCTCC 3 cut(s) 43, 753, 1637
Lsp1109I GCAGC 5 cut(s) 613, 616, 694, 853, 1699
LweI GCATC 3 cut(s) 694, 952, 1483
MaeII ACGT 2 cut(s) 1365, 1627
MaeIII GTNAC 2 cut(s) 842, 1531
MbiI CCGCTC 2 cut(s) 21, 1470
MflI RGATCY 3 cut(s) 1175, 1220, 1651
MhlI GDGCHC 3 cut(s) 260, 1036, 1642
MluCI AATT 3 cut(s) 181, 228, 1392
MmeI TCCRAC 5 cut(s) 50, 434, 1092, 1577, 1593
Mph1103I ATGCAT 1 cut(s) 1164
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 6 cut(s) 231, 414, 428, 446, 576, 1001
MspA1I CMGCKG 1 cut(s) 866
MspI CCGG 2 cut(s) 670, 1087
MspR9I CCNGG 2 cut(s) 670, 1272
Mva1269I GAATGC 2 cut(s) 514, 916
MvaI CCWGG 1 cut(s) 1272
MwoI GCNNNNNNNGC 2 cut(s) 962, 1712
NciI CCSGG 1 cut(s) 670
NdeI CATATG 1 cut(s) 1164
NlaIII CATG 2 cut(s) 372, 398
NlaIV GGNNCC 4 cut(s) 45, 674, 1033, 1653
NsiI ATGCAT 1 cut(s) 1164
PaeR7I CTCGAG 1 cut(s) 406
PaqCI CACCTGC 1 cut(s) 1396
PctI GAATGC 2 cut(s) 514, 916
PdmI GAANNNNTTC 1 cut(s) 554
PflMI CCANNNNNTGG 2 cut(s) 374, 1676
PkrI GCNGC 6 cut(s) 20, 628, 631, 709, 868, 1714
PshBI ATTAAT 1 cut(s) 231
Psp124BI GAGCTC 1 cut(s) 1642
Psp1406I AACGTT 1 cut(s) 1365
Psp6I CCWGG 1 cut(s) 1270
PspFI CCCAGC 3 cut(s) 151, 485, 653
PspGI CCWGG 1 cut(s) 1270
PspN4I GGNNCC 4 cut(s) 45, 674, 1033, 1653
PspPI GGNCC 1 cut(s) 494
PspXI VCTCGAGB 1 cut(s) 406
PsrI GAACNNNNNNTAC 2 cut(s) 1316, 1348
PsuI RGATCY 3 cut(s) 1175, 1220, 1651
PvuII CAGCTG 1 cut(s) 866
RsaI GTAC 6 cut(s) 36, 134, 163, 674, 1512, 1567
RsaNI GTAC 6 cut(s) 35, 133, 162, 673, 1511, 1566
SacI GAGCTC 1 cut(s) 1642
SaqAI TTAA 6 cut(s) 231, 414, 428, 446, 576, 1001
SatI GCNGC 6 cut(s) 19, 627, 630, 708, 867, 1713
Sau96I GGNCC 1 cut(s) 494
ScrFI CCNGG 2 cut(s) 670, 1272
SduI GDGCHC 3 cut(s) 260, 1036, 1642
SfaNI GCATC 3 cut(s) 694, 952, 1483
SfcI CTRYAG 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 406
SlaI CTCGAG 1 cut(s) 406
SmlI CTYRAG 2 cut(s) 406, 503
SmoI CTYRAG 2 cut(s) 406, 503
Sse9I AATT 3 cut(s) 181, 228, 1392
SsiI CCGC 4 cut(s) 19, 314, 1196, 1468
SstI GAGCTC 1 cut(s) 1642
StyD4I CCNGG 2 cut(s) 668, 1270
TaaI ACNGT 3 cut(s) 166, 349, 1595
TaiI ACGT 2 cut(s) 1368, 1630
TaqI TCGA 7 cut(s) 351, 407, 717, 1390, 1557, 1584, 1664
TasI AATT 3 cut(s) 181, 228, 1392
TatI WGTACW 2 cut(s) 132, 1510
TauI GCSGC 1 cut(s) 21
Tru1I TTAA 6 cut(s) 231, 414, 428, 446, 576, 1001
Tru9I TTAA 6 cut(s) 231, 414, 428, 446, 576, 1001
TscAI CASTG 3 cut(s) 265, 1088, 1335
TseI GCWGC 5 cut(s) 626, 629, 707, 866, 1712
TspRI CASTG 3 cut(s) 265, 1088, 1335
Van91I CCANNNNNTGG 2 cut(s) 374, 1676
VspI ATTAAT 1 cut(s) 231
XhoI CTCGAG 1 cut(s) 406
XmnI GAANNNNTTC 1 cut(s) 554
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.