MD05G1208000.v1.1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0022293
Physical Location & Seq
Reverse (-)
33927590 .. 33928165
576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1208000.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGGGAGGACTGGATTGTTTGATCTTGAAAAGCAGTTTGCCTTCTATGGAGCATATCACAACAACCCAATTAACATTTTCATACACACTTTCTTGGTGTGGCCATTGTTCTTCACTTCTGTTCTTCTTTTCCACTTCACACCTTCTCTCTACAGTTTCCCAGTTTATGGCCTCGAATTAAATTTGGGGTTTGTTTTCACTTTGGCGTATTGTCTGTATTATGTCAAATTGGACAAGAAAGCTGGGTCCTTGGCAGCTTTGATTTGTATTCTCTGCTGGGTTGGAGCCAGTATTCTTGGCAGTGAGCTAGGGTTTTCTAAGTCGTGGAAGGTGGTTCTGGGTGGTCAGTTCTGCAGTTGGGCTGGACAGGTCGTAGGCCATGGGATTTTTGAGAAGAGGGCCCCTTCAGACAACTTTCTTGAAGGACTTCTAACAGGGCCGTACTTTGTGTTGCTTGAGCTTCTTCACTTAGCTTTTGGGTACGAGCCCTATCCAGGGTTTCAAAAAAGTGTGAAGGCAAAGATTGAAGCTGATATCAAAGATTGGAAGGCCAAGAACCAAAAGAAAGTCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

192

Amino Acids

21.52

Weight (kDa)

8.8

Isoelectric Point (pI)

22.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 155 2.8e-15 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022293)

Species Orthologous Gene IDs
malus_domestica MD05G1208000.v1.1 MD10G1194700.v1.1
prunus_persica Prupe.4G149500_v2.0.a1
pyrus_communis pycom05g19390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 167
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 181
AcuI CTGAAG 1 cut(s) 390
AfaI GTAC 2 cut(s) 443, 482
AfiI CCNNNNNNNGG 3 cut(s) 167, 494, 495
AgsI TTSAA 4 cut(s) 29, 422, 503, 527
AjnI CCWGG 1 cut(s) 493
AluBI AGCT 6 cut(s) 242, 257, 307, 460, 473, 530
AluI AGCT 6 cut(s) 242, 257, 307, 460, 473, 530
AoxI GGCC 6 cut(s) 101, 169, 376, 399, 437, 549
ApaI GGGCCC 1 cut(s) 403
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 1 cut(s) 181
Asp700I GAANNNNTTC 1 cut(s) 426
AspS9I GGNCC 4 cut(s) 246, 399, 400, 437
AvaII GGWCC 1 cut(s) 246
BaeGI GKGCMC 1 cut(s) 403
BalI TGGCCA 1 cut(s) 103
BanII GRGCYC 2 cut(s) 403, 489
BbvI GCAGC 1 cut(s) 266
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 1 cut(s) 495
BfaI CTAG 2 cut(s) 308, 574
BfmI CTRYAG 2 cut(s) 151, 352
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 4 cut(s) 246, 399, 400, 437
BmiI GGNNCC 4 cut(s) 247, 286, 401, 402
BmrFI CCNGG 1 cut(s) 495
BmrI ACTGGG 1 cut(s) 155
BmuI ACTGGG 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 476
BsaJI CCNNGG 3 cut(s) 249, 379, 494
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 494, 495
Bse1I ACTGG 3 cut(s) 16, 161, 288
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 3 cut(s) 249, 379, 494
BseLI CCNNNNNNNGG 3 cut(s) 167, 494, 495
BseNI ACTGG 3 cut(s) 16, 161, 288
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 1 cut(s) 266
BseYI CCCAGC 2 cut(s) 242, 276
BshFI GGCC 6 cut(s) 103, 171, 378, 401, 439, 551
BslI CCNNNNNNNGG 3 cut(s) 167, 494, 495
BsnI GGCC 6 cut(s) 103, 171, 378, 401, 439, 551
Bsp120I GGGCCC 1 cut(s) 399
Bsp1286I GDGCHC 2 cut(s) 403, 489
Bsp143I GATC 1 cut(s) 22
Bsp19I CCATGG 1 cut(s) 379
BspANI GGCC 6 cut(s) 103, 171, 378, 401, 439, 551
BspLI GGNNCC 4 cut(s) 247, 286, 401, 402
BspMAI CTGCAG 1 cut(s) 356
BsrI ACTGG 3 cut(s) 16, 161, 288
BssECI CCNNGG 3 cut(s) 249, 379, 494
BssMI GATC 1 cut(s) 22
BssT1I CCWWGG 2 cut(s) 249, 379
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 318, 469
BstDSI CCRYGG 1 cut(s) 379
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstNI CCWGG 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 2 cut(s) 151, 352
BstSLI GKGCMC 1 cut(s) 403
BstV1I GCAGC 1 cut(s) 266
BsuRI GGCC 6 cut(s) 103, 171, 378, 401, 439, 551
BtgI CCRYGG 1 cut(s) 379
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 1 cut(s) 307
Cfr13I GGNCC 4 cut(s) 246, 399, 400, 437
Csp6I GTAC 2 cut(s) 442, 481
CviAII CATG 1 cut(s) 380
CviQI GTAC 2 cut(s) 442, 481
DdeI CTNAG 2 cut(s) 318, 469
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Eco130I CCWWGG 2 cut(s) 249, 379
Eco24I GRGCYC 2 cut(s) 403, 489
Eco32I GATATC 1 cut(s) 535
Eco47I GGWCC 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 390
EcoO109I RGGNCCY 3 cut(s) 246, 399, 400
EcoRII CCWGG 1 cut(s) 493
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 2 cut(s) 249, 379
EcoT38I GRGCYC 2 cut(s) 403, 489
ErhI CCWWGG 2 cut(s) 249, 379
FaeI CATG 1 cut(s) 383
FaiI YATR 6 cut(s) 48, 55, 83, 168, 222, 381
FatI CATG 1 cut(s) 379
Fnu4HI GCNGC 1 cut(s) 255
FriOI GRGCYC 2 cut(s) 403, 489
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 2 cut(s) 308, 574
GluI GCNGC 1 cut(s) 255
GsaI CCCAGC 2 cut(s) 246, 280
HaeIII GGCC 6 cut(s) 103, 171, 378, 401, 439, 551
Hin1II CATG 1 cut(s) 383
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 2 cut(s) 26, 419
HpyAV CCTTC 7 cut(s) 52, 153, 322, 414, 416, 508, 541
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4V TGCA 1 cut(s) 354
HpyF3I CTNAG 2 cut(s) 318, 469
Hsp92II CATG 1 cut(s) 383
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 2 cut(s) 50, 284
Lsp1109I GCAGC 1 cut(s) 266
MaeI CTAG 2 cut(s) 308, 574
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 4 cut(s) 103, 116, 406, 455
MhlI GDGCHC 2 cut(s) 403, 489
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 4 cut(s) 69, 176, 181, 227
MluNI TGGCCA 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 2 cut(s) 182, 390
Mox20I TGGCCA 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 426
MscI TGGCCA 1 cut(s) 103
MseI TTAA 2 cut(s) 72, 179
Msp20I TGGCCA 1 cut(s) 103
MspR9I CCNGG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 495
NcoI CCATGG 1 cut(s) 379
NdeII GATC 1 cut(s) 22
NlaIII CATG 1 cut(s) 383
NlaIV GGNNCC 4 cut(s) 247, 286, 401, 402
PdmI GAANNNNTTC 1 cut(s) 426
PflMI CCANNNNNTGG 1 cut(s) 167
PkrI GCNGC 1 cut(s) 256
PpuMI RGGWCCY 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 493
PspFI CCCAGC 2 cut(s) 242, 276
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 4 cut(s) 247, 286, 401, 402
PspOMI GGGCCC 1 cut(s) 399
PspPI GGNCC 4 cut(s) 246, 399, 400, 437
PspPPI RGGWCCY 1 cut(s) 246
PstI CTGCAG 1 cut(s) 356
RsaI GTAC 2 cut(s) 443, 482
RsaNI GTAC 2 cut(s) 442, 481
SaqAI TTAA 2 cut(s) 72, 179
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 4 cut(s) 246, 399, 400, 437
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 2 cut(s) 403, 489
SetI ASST 9 cut(s) 145, 244, 259, 309, 333, 372, 462, 475, 532
SfcI CTRYAG 2 cut(s) 151, 352
SinI GGWCC 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 4 cut(s) 69, 176, 181, 227
SspMI CTAG 2 cut(s) 308, 574
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 2 cut(s) 249, 379
TaaI ACNGT 1 cut(s) 155
TaqI TCGA 1 cut(s) 174
TasI AATT 4 cut(s) 69, 176, 181, 227
Tru1I TTAA 2 cut(s) 72, 179
Tru9I TTAA 2 cut(s) 72, 179
TscAI CASTG 1 cut(s) 307
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 70
TspRI CASTG 1 cut(s) 307
Van91I CCANNNNNTGG 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 1 cut(s) 181
XmnI GAANNNNTTC 1 cut(s) 426
XspI CTAG 2 cut(s) 308, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.