Prupe.4G149500_v2.0.a1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0022293
Physical Location & Seq
Forward (+)
8529268 .. 8530590
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G149500.1

Sequence Viewer

Length: 591 bp
ATGGGTAGGTCTGGATTGTTTGATCTTGAGAGGCACTTTGCCTTCTATGGAGCATATCACAGCAACCCTTTTAACATTTTGATACACACTTTCCTTGTGTGGCCTTTGTTCTTCACTTCCGTCCTTCTTTTCCACTTCACACCTTCTGTCTACAGTTTCCCATCTGGGTTTAGTGAGCACGGACTTGAAATGAATTTTGGGTTTGTTTTCACTGTGATGTATGCTCTGTACTATGTTGGCTTGGATAAGAAAGCAGGGTCCTTGGCAGCTTTGCTCTGTTTTGTCAATTGGGTTGGAGCCAGTGTTCTTGGTGGTGAGCTTGGGTTTTCAAAATCATGGAAGGTTGTTCTGATTGGTCAGTTGCTCTCCTGGGCTGGACAGATCATAGGCCATGGGGTTTTTGAGAAAAGAGCTCCTTCAGAAAACTTTGCTGAAGGGCTTCTGATGGGGCCTTACTTTGTGCTGCTTGAGCTTCTTCACTTCGCCTTTGGCTATGAACCTTATCCAGGGTTTTACAAAAGTGTGAATGCAAAGATTGATGCTGATATCGCAGAGTGGAAGGTGGCCAAGAACCAAAAGAAACTCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

197

Amino Acids

22.02

Weight (kDa)

7.06

Isoelectric Point (pI)

22.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022293)

Species Orthologous Gene IDs
malus_domestica MD05G1208000.v1.1 MD10G1194700.v1.1
prunus_persica Prupe.4G149500_v2.0.a1
pyrus_communis pycom05g19390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 150
AcoI YGGCCR 1 cut(s) 564
AcsI RAATTY 1 cut(s) 193
AcuI CTGAAG 2 cut(s) 402, 453
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 1 cut(s) 506
AgsI TTSAA 2 cut(s) 188, 330
AjnI CCWGG 2 cut(s) 368, 505
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 4 cut(s) 269, 319, 413, 472
AluI AGCT 4 cut(s) 269, 319, 413, 472
Alw21I GWGCWC 2 cut(s) 180, 415
AoxI GGCC 4 cut(s) 101, 388, 449, 564
ApeKI GCWGC 2 cut(s) 266, 463
ApoI RAATTY 1 cut(s) 193
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 258, 449
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 1 cut(s) 258
BalI TGGCCA 1 cut(s) 566
BanII GRGCYC 1 cut(s) 415
Bbv12I GWGCWC 2 cut(s) 180, 415
BbvI GCAGC 2 cut(s) 278, 450
BccI CCATC 2 cut(s) 169, 439
BciT130I CCWGG 2 cut(s) 370, 507
BfmI CTRYAG 1 cut(s) 151
BisI GCNGC 2 cut(s) 267, 464
BlsI GCNGC 2 cut(s) 268, 465
Bme1390I CCNGG 2 cut(s) 370, 507
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 258, 449
BmiI GGNNCC 3 cut(s) 259, 298, 450
BmrFI CCNGG 2 cut(s) 370, 507
BmsI GCATC 1 cut(s) 529
BpuEI CTTGAG 2 cut(s) 47, 488
BsaJI CCNNGG 4 cut(s) 261, 369, 391, 506
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse1I ACTGG 1 cut(s) 300
BseBI CCWGG 2 cut(s) 370, 507
BseDI CCNNGG 4 cut(s) 261, 369, 391, 506
BseLI CCNNNNNNNGG 1 cut(s) 506
BseNI ACTGG 1 cut(s) 300
BseXI GCAGC 2 cut(s) 278, 450
BshFI GGCC 4 cut(s) 103, 390, 451, 566
BsiHKAI GWGCWC 2 cut(s) 180, 415
BslI CCNNNNNNNGG 1 cut(s) 506
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 4 cut(s) 103, 390, 451, 566
Bsp1286I GDGCHC 2 cut(s) 180, 415
Bsp143I GATC 2 cut(s) 22, 381
Bsp19I CCATGG 1 cut(s) 391
BspANI GGCC 4 cut(s) 103, 390, 451, 566
BspLI GGNNCC 3 cut(s) 259, 298, 450
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 4 cut(s) 261, 369, 391, 506
BssMI GATC 2 cut(s) 22, 381
BssT1I CCWWGG 2 cut(s) 261, 391
Bst2UI CCWGG 2 cut(s) 370, 507
Bst4CI ACNGT 2 cut(s) 155, 214
BstDSI CCRYGG 1 cut(s) 391
BstENI CCTNNNNNAGG 1 cut(s) 504
BstKTI GATC 2 cut(s) 25, 384
BstMBI GATC 2 cut(s) 22, 381
BstMWI GCNNNNNNNGC 2 cut(s) 469, 548
BstNI CCWGG 2 cut(s) 370, 507
BstSCI CCNGG 2 cut(s) 368, 505
BstSFI CTRYAG 1 cut(s) 151
BstV1I GCAGC 2 cut(s) 278, 450
BsuRI GGCC 4 cut(s) 103, 390, 451, 566
BtgI CCRYGG 1 cut(s) 391
BtsIMutI CAGTG 2 cut(s) 210, 307
Cfr13I GGNCC 2 cut(s) 258, 449
Csp6I GTAC 1 cut(s) 229
CviAII CATG 2 cut(s) 336, 392
CviQI GTAC 1 cut(s) 229
DpnI GATC 2 cut(s) 24, 383
DpnII GATC 2 cut(s) 22, 381
EaeI YGGCCR 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 413
Eco130I CCWWGG 2 cut(s) 261, 391
Eco24I GRGCYC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 547
Eco47I GGWCC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 402, 453
EcoICRI GAGCTC 1 cut(s) 413
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoO109I RGGNCCY 2 cut(s) 258, 449
EcoRII CCWGG 2 cut(s) 368, 505
EcoRV GATATC 1 cut(s) 547
EcoT14I CCWWGG 2 cut(s) 261, 391
EcoT38I GRGCYC 1 cut(s) 415
ErhI CCWWGG 2 cut(s) 261, 391
FaeI CATG 2 cut(s) 339, 395
FaiI YATR 8 cut(s) 48, 55, 222, 234, 337, 386, 393, 495
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FatI CATG 2 cut(s) 335, 391
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 267, 464
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 2 cut(s) 267, 464
GluI GCNGC 2 cut(s) 267, 464
HaeIII GGCC 4 cut(s) 103, 390, 451, 566
Hin1II CATG 2 cut(s) 339, 395
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 3 cut(s) 351, 421, 444
Hpy188III TCNNGA 2 cut(s) 12, 26
Hpy8I GTNNAC 1 cut(s) 151
HpyAV CCTTC 7 cut(s) 52, 134, 153, 334, 426, 428, 553
HpyCH4III ACNGT 2 cut(s) 155, 214
HpyCH4V TGCA 1 cut(s) 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 469, 548
Hsp92II CATG 2 cut(s) 339, 395
Kzo9I GATC 2 cut(s) 22, 381
LmnI GCTCC 3 cut(s) 50, 296, 418
LpnPI CCDG 8 cut(s) 150, 240, 313, 355, 360, 382, 492, 519
Lsp1109I GCAGC 2 cut(s) 278, 450
LweI GCATC 1 cut(s) 529
MalI GATC 2 cut(s) 24, 383
MboI GATC 2 cut(s) 22, 381
MboII GAAGA 2 cut(s) 103, 467
MfeI CAATTG 1 cut(s) 286
MhlI GDGCHC 2 cut(s) 180, 415
MlsI TGGCCA 1 cut(s) 566
MluCI AATT 2 cut(s) 193, 286
MluNI TGGCCA 1 cut(s) 566
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 1 cut(s) 24
Mox20I TGGCCA 1 cut(s) 566
MroXI GAANNNNTTC 1 cut(s) 438
MscI TGGCCA 1 cut(s) 566
MseI TTAA 2 cut(s) 72, 589
MslI CAYNNNNRTG 1 cut(s) 215
Msp20I TGGCCA 1 cut(s) 566
MspR9I CCNGG 2 cut(s) 370, 507
MunI CAATTG 1 cut(s) 286
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 2 cut(s) 370, 507
MwoI GCNNNNNNNGC 2 cut(s) 469, 548
NcoI CCATGG 1 cut(s) 391
NdeII GATC 2 cut(s) 22, 381
NlaIII CATG 2 cut(s) 339, 395
NlaIV GGNNCC 3 cut(s) 259, 298, 450
PctI GAATGC 1 cut(s) 532
PdmI GAANNNNTTC 1 cut(s) 438
PkrI GCNGC 2 cut(s) 268, 465
PpuMI RGGWCCY 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 368, 505
PspGI CCWGG 2 cut(s) 368, 505
PspN4I GGNNCC 3 cut(s) 259, 298, 450
PspPI GGNCC 2 cut(s) 258, 449
PspPPI RGGWCCY 1 cut(s) 258
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 215
SacI GAGCTC 1 cut(s) 415
SaqAI TTAA 2 cut(s) 72, 589
SatI GCNGC 2 cut(s) 267, 464
Sau3AI GATC 2 cut(s) 22, 381
Sau96I GGNCC 2 cut(s) 258, 449
ScrFI CCNGG 2 cut(s) 370, 507
SduI GDGCHC 2 cut(s) 180, 415
SetI ASST 9 cut(s) 11, 145, 271, 321, 345, 415, 474, 502, 564
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 1 cut(s) 151
SinI GGWCC 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 2 cut(s) 26, 467
SmoI CTYRAG 2 cut(s) 26, 467
Sse9I AATT 2 cut(s) 193, 286
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 2 cut(s) 368, 505
StyI CCWWGG 2 cut(s) 261, 391
TaaI ACNGT 2 cut(s) 155, 214
TasI AATT 2 cut(s) 193, 286
TatI WGTACW 1 cut(s) 228
Tru1I TTAA 2 cut(s) 72, 589
Tru9I TTAA 2 cut(s) 72, 589
TscAI CASTG 2 cut(s) 217, 307
TseI GCWGC 2 cut(s) 266, 463
TspDTI ATGAA 2 cut(s) 206, 510
TspGWI ACGGA 2 cut(s) 109, 195
TspRI CASTG 2 cut(s) 217, 307
VpaK11BI GGWCC 1 cut(s) 258
XagI CCTNNNNNAGG 1 cut(s) 504
XapI RAATTY 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.