MD10G1194700.v1.1

Endoplasmic reticulum membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0022293
Physical Location & Seq
Reverse (-)
29278312 .. 29278887
576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1194700.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGGGAGGACTGGATTGTTTGATCTTGAAAGGAACTTTGCCTTCCATGGAGCATATCACAACAACCCAATTAACATTTTCATACACACTTTCTTGGTGTGGCCATTGTTCTTCACTTCTGTTCTTCTTTTCCACTTCACACCTTCCCTCTACAGTTTCCCAGTTCATGGCCTCGAATTAAATTTGGGGTTTGTTTTCACGTTGGCGTATTGTTTGTATTACATCAAATTGGACAAGAAAGCTGGGTCCTTGGCAGCTTTGATCTGTATTCTCTGCTGGGTTGGAGCCAGTGTTCTTGGCAGTGAGCTAGGGTTTTCTAAGTCGTGGAAGGTTGTTCTGGGTGGTCAGTTCTGCAGTTGGGCTGGACAGGTCATAGGCCACGGGATATTTGAAAAGAGAGCCCCTTCAAACAACTTTCTTCAAGGACTTCTAATGGGGCCGTACTTTGTGTTGCTGGAGCTACTTCACTCAACTTTTGGGTATGAACCCTATCCAGGGTTTCATAAAAGTGTTACGGCAAAGATTGAAGCTGATATCAAAGATTGGAAGGCCAAGAACCAAAAGAAAGTCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF962

Protein Analysis

192

Amino Acids

21.51

Weight (kDa)

9.02

Isoelectric Point (pI)

22.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpo1-like PF06127 8 - 156 4.9e-14 2-hydroxy-palmitic acid dioxygenase Mpo1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022293)

Species Orthologous Gene IDs
malus_domestica MD05G1208000.v1.1 MD10G1194700.v1.1
prunus_persica Prupe.4G149500_v2.0.a1
pyrus_communis pycom05g19390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 167
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 181
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 3 cut(s) 167, 494, 495
AgsI TTSAA 5 cut(s) 29, 392, 408, 422, 527
AjnI CCWGG 1 cut(s) 493
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
AluBI AGCT 5 cut(s) 242, 257, 307, 460, 530
AluI AGCT 5 cut(s) 242, 257, 307, 460, 530
AoxI GGCC 5 cut(s) 101, 169, 376, 437, 549
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 1 cut(s) 181
AspS9I GGNCC 2 cut(s) 246, 437
AvaII GGWCC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 103
BanII GRGCYC 1 cut(s) 403
BbvI GCAGC 1 cut(s) 266
BceAI ACGGC 2 cut(s) 424, 531
BciT130I CCWGG 1 cut(s) 495
BfaI CTAG 1 cut(s) 308
BfmI CTRYAG 2 cut(s) 151, 352
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 2 cut(s) 246, 437
BmiI GGNNCC 3 cut(s) 247, 286, 438
BmrFI CCNGG 1 cut(s) 495
BmrI ACTGGG 1 cut(s) 155
BmuI ACTGGG 1 cut(s) 155
BpmI CTGGAG 1 cut(s) 476
BsaJI CCNNGG 4 cut(s) 46, 249, 379, 494
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 494, 495
Bse1I ACTGG 3 cut(s) 16, 161, 288
BseBI CCWGG 1 cut(s) 495
BseDI CCNNGG 4 cut(s) 46, 249, 379, 494
BseLI CCNNNNNNNGG 3 cut(s) 167, 494, 495
BseNI ACTGG 3 cut(s) 16, 161, 288
BseXI GCAGC 1 cut(s) 266
BseYI CCCAGC 2 cut(s) 242, 276
BshFI GGCC 5 cut(s) 103, 171, 378, 439, 551
BslI CCNNNNNNNGG 3 cut(s) 167, 494, 495
BsnI GGCC 5 cut(s) 103, 171, 378, 439, 551
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 2 cut(s) 22, 261
Bsp19I CCATGG 1 cut(s) 46
BspANI GGCC 5 cut(s) 103, 171, 378, 439, 551
BspLI GGNNCC 3 cut(s) 247, 286, 438
BspMAI CTGCAG 1 cut(s) 356
BsrI ACTGG 3 cut(s) 16, 161, 288
BssECI CCNNGG 4 cut(s) 46, 249, 379, 494
BssMI GATC 2 cut(s) 22, 261
BssT1I CCWWGG 2 cut(s) 46, 249
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 155
BstDEI CTNAG 1 cut(s) 318
BstDSI CCRYGG 2 cut(s) 46, 379
BstKTI GATC 2 cut(s) 25, 264
BstMBI GATC 2 cut(s) 22, 261
BstNI CCWGG 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 2 cut(s) 151, 352
BstV1I GCAGC 1 cut(s) 266
BsuRI GGCC 5 cut(s) 103, 171, 378, 439, 551
BtgI CCRYGG 2 cut(s) 46, 379
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 295, 307
Cfr13I GGNCC 2 cut(s) 246, 437
Csp6I GTAC 1 cut(s) 442
CviAII CATG 2 cut(s) 47, 167
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 24, 263
DpnII GATC 2 cut(s) 22, 261
EaeI YGGCCR 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 46, 249
Eco24I GRGCYC 1 cut(s) 403
Eco32I GATATC 1 cut(s) 535
Eco47I GGWCC 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 246
EcoRII CCWGG 1 cut(s) 493
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 2 cut(s) 46, 249
EcoT38I GRGCYC 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 46, 249
FaeI CATG 2 cut(s) 50, 170
FaiI YATR 7 cut(s) 48, 55, 83, 168, 374, 483, 504
FatI CATG 2 cut(s) 46, 166
Fnu4HI GCNGC 1 cut(s) 255
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 1 cut(s) 308
GluI GCNGC 1 cut(s) 255
GsaI CCCAGC 2 cut(s) 246, 280
GsuI CTGGAG 1 cut(s) 476
HaeIII GGCC 5 cut(s) 103, 171, 378, 439, 551
Hin1II CATG 2 cut(s) 50, 170
Hpy188III TCNNGA 1 cut(s) 26
HpyAV CCTTC 5 cut(s) 52, 153, 322, 414, 541
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 1 cut(s) 354
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 200
Hsp92II CATG 2 cut(s) 50, 170
Kzo9I GATC 2 cut(s) 22, 261
LmnI GCTCC 3 cut(s) 50, 284, 457
Lsp1109I GCAGC 1 cut(s) 266
MaeI CTAG 1 cut(s) 308
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 511
MalI GATC 2 cut(s) 24, 263
MboI GATC 2 cut(s) 22, 261
MboII GAAGA 3 cut(s) 103, 116, 410
MhlI GDGCHC 1 cut(s) 403
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 4 cut(s) 69, 176, 181, 227
MluNI TGGCCA 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 2 cut(s) 158, 182
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
MseI TTAA 2 cut(s) 72, 179
MslI CAYNNNNRTG 1 cut(s) 507
Msp20I TGGCCA 1 cut(s) 103
MspR9I CCNGG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 495
NcoI CCATGG 1 cut(s) 46
NdeII GATC 2 cut(s) 22, 261
NlaIII CATG 2 cut(s) 50, 170
NlaIV GGNNCC 3 cut(s) 247, 286, 438
PflMI CCANNNNNTGG 1 cut(s) 167
PkrI GCNGC 1 cut(s) 256
PpuMI RGGWCCY 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 493
PspFI CCCAGC 2 cut(s) 242, 276
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 3 cut(s) 247, 286, 438
PspPI GGNCC 2 cut(s) 246, 437
PspPPI RGGWCCY 1 cut(s) 246
PstI CTGCAG 1 cut(s) 356
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
RseI CAYNNNNRTG 1 cut(s) 507
SaqAI TTAA 2 cut(s) 72, 179
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 2 cut(s) 22, 261
Sau96I GGNCC 2 cut(s) 246, 437
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 1 cut(s) 403
SetI ASST 9 cut(s) 145, 203, 244, 259, 309, 333, 372, 462, 532
SfcI CTRYAG 2 cut(s) 151, 352
SinI GGWCC 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 507
Sse9I AATT 4 cut(s) 69, 176, 181, 227
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 2 cut(s) 46, 249
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 1 cut(s) 203
TaqI TCGA 1 cut(s) 174
TasI AATT 4 cut(s) 69, 176, 181, 227
Tru1I TTAA 2 cut(s) 72, 179
Tru9I TTAA 2 cut(s) 72, 179
TscAI CASTG 2 cut(s) 295, 307
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 4 cut(s) 70, 155, 491, 498
TspRI CASTG 2 cut(s) 295, 307
Van91I CCANNNNNTGG 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 1 cut(s) 181
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.