MD05G1251200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024464
Physical Location & Seq
Reverse (-)
38305734 .. 38306479
746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1251200.v1.1.491

Sequence Viewer

Length: 309 bp
ATGGTAGATGCATATGGAAGCGTTGGTCTTCATGAAGATGCACAAGCTACGTTTGAAGAGATGAAGCGATTAGGAATAACCCCGACAGAGAAATCCCATATGCTACTTTTATCCGCCTACTCCAAAGCTGGCAACGTAGCTAAATGTGAGGACATTGTTAACCAGATACAAGTGTCCGGGCTGGAGCTAGACACTTTCGTCATCAACAGCATATTAAATCTGTATGGCCGGTTGGGTCAGTTAGAAAAGATGGAGGAAGTTATGACAGCAATGGAAAAAGGACCTTCTGCAGCTGATATCAGTACATAA

Protein Analysis

103

Amino Acids

11.09

Weight (kDa)

4.63

Isoelectric Point (pI)

23.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 13 - 91 5e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024464)

Species Orthologous Gene IDs
malus_domestica MD05G1251200.v1.1
pyrus_communis pycom17g12520
rosa_multiflora Rmu_sc0006887.1_g000012

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AciI CCGC 1 cut(s) 114
AcoI YGGCCR 1 cut(s) 226
AfaI GTAC 1 cut(s) 304
AgsI TTSAA 1 cut(s) 56
AluBI AGCT 5 cut(s) 47, 128, 140, 187, 293
AluI AGCT 5 cut(s) 47, 128, 140, 187, 293
AoxI GGCC 1 cut(s) 226
ApeKI GCWGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 281
AsuC2I CCSGG 1 cut(s) 178
AvaII GGWCC 1 cut(s) 281
BaeI ACNNNNGTAYC 2 cut(s) 158, 191
BbsI GAAGAC 1 cut(s) 20
BbvI GCAGC 1 cut(s) 302
BccI CCATC 1 cut(s) 244
BcnI CCSGG 1 cut(s) 178
BfaI CTAG 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 288
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 178
BmsI GCATC 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 20
BpmI CTGGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 276
BseMI GCAATG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 302
BshFI GGCC 1 cut(s) 228
BsiSI CCGG 2 cut(s) 177, 229
BsnI GGCC 1 cut(s) 228
BspACI CCGC 1 cut(s) 114
BspANI GGCC 1 cut(s) 228
BspHI TCATGA 1 cut(s) 31
BspMAI CTGCAG 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 276
BsrFI RCCGGY 1 cut(s) 228
BssAI RCCGGY 1 cut(s) 228
Bst6I CTCTTC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 130
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 302
BstV2I GAAGAC 1 cut(s) 20
BsuRI GGCC 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 130
CciI TCATGA 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 303
CviAII CATG 1 cut(s) 32
CviJI RGCY 7 cut(s) 47, 128, 140, 181, 187, 228, 293
CviKI_1 RGCY 7 cut(s) 47, 128, 140, 181, 187, 228, 293
CviQI GTAC 1 cut(s) 303
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 1 cut(s) 103
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 281
EcoRV GATATC 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 13
FaeI CATG 1 cut(s) 35
FaiI YATR 9 cut(s) 13, 15, 33, 99, 101, 212, 225, 263, 307
FatI CATG 1 cut(s) 31
FauNDI CATATG 2 cut(s) 13, 99
Fnu4HI GCNGC 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 1 cut(s) 188
GluI GCNGC 1 cut(s) 291
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 1 cut(s) 228
HapII CCGG 2 cut(s) 177, 229
Hin1II CATG 1 cut(s) 35
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 2 cut(s) 177, 229
Hpy166II GTNNAC 1 cut(s) 160
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 294
HpyCH4IV ACGT 2 cut(s) 50, 135
HpyCH4V TGCA 3 cut(s) 11, 41, 290
HpySE526I ACGT 2 cut(s) 50, 135
Hsp92II CATG 1 cut(s) 35
KspAI GTTAAC 1 cut(s) 160
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 5 cut(s) 114, 167, 176, 190, 242
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 1 cut(s) 28
MaeI CTAG 1 cut(s) 188
MaeII ACGT 2 cut(s) 50, 135
MboII GAAGA 3 cut(s) 20, 47, 68
MnlI CCTC 2 cut(s) 142, 247
Mph1103I ATGCAT 1 cut(s) 13
MseI TTAA 2 cut(s) 159, 215
MslI CAYNNNNRTG 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 2 cut(s) 177, 229
MspR9I CCNGG 1 cut(s) 178
NciI CCSGG 1 cut(s) 178
NdeI CATATG 2 cut(s) 13, 99
NlaIII CATG 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 13
PagI TCATGA 1 cut(s) 31
PkrI GCNGC 1 cut(s) 292
PpuMI RGGWCCY 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 281
PspPI GGNCC 1 cut(s) 281
PspPPI RGGWCCY 1 cut(s) 281
PstI CTGCAG 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 2 cut(s) 159, 215
SatI GCNGC 1 cut(s) 291
Sau96I GGNCC 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 178
SetI ASST 8 cut(s) 49, 53, 130, 138, 142, 189, 286, 295
SfaNI GCATC 1 cut(s) 28
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 36
SsiI CCGC 1 cut(s) 114
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 176
TaiI ACGT 2 cut(s) 53, 138
TatI WGTACW 1 cut(s) 302
Tru1I TTAA 2 cut(s) 159, 215
Tru9I TTAA 2 cut(s) 159, 215
TseI GCWGC 1 cut(s) 290
TspDTI ATGAA 3 cut(s) 20, 48, 77
VpaK11BI GGWCC 1 cut(s) 281
XspI CTAG 1 cut(s) 188
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.