MD05G1300600.v1.1

Pleckstrin homology domain-containing family A member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024453
Physical Location & Seq
Forward (+)
43366038 .. 43370192
4155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1300600.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGTCAAAGAGAAAAGCGGCAGCGGCAGGGCCAATAAAAGAGAGTTCGCAGACAACCAAAGTAAAAGAAACAGACGTTTCCCTTTCGGCATGGATAAGGAAACAGGAATCCTTTGTGGCAAATTATACTATTATCTTGCCGTTGGGCGGTCCTGCTCCGGATCGGGAGAGCTTGTTCACTCCTGTTTTGGAAATGTTGGAGTTGGTCAGGTCCCCAAGTGGAGAGATTCGGACCTTGCCTTTCTTGGTAGCGTGCAGGCATACTGCACCAATTACACTTCAATTTGGAGAACCTTTGAATCATGTTAGGACATCCATCTTTGCTTGTGCAGTGCCAATGGAAAGAATATATCAATCCAATCCTTCCAAGTTCGAATACTTGTACAGTTTGGTGAACCATGATGTCAAAACAAATACTCTTTTCAATAGTACCTCCGTTTCTCTTGTTTGGCTCACCAGATACATGGATTTTTTGGTAGAGCTGTTTCGGAACTTACGTGAGCATCCTCCAGATTGGGTTACGATTCGAGCTGTTTATAATGCCTATGCCAAGACCCTGATAAAATGGCATGATGAAAAAATTCTTGGAAACAAATATGCTCGCGAAAAAGACTTGTTGTTGAAGGTGATCGGAGGCAATGGTGATGTCATGGGAGACATGGAGAAATTTTGCACTTCCTTTACTCCTCTACTTAAACAGAATCACGAGTTTTTGGCTGGAGTGGATGATTTGAAGTTATTTCGTGCTAGCCTTGTAGCAAAGGTACCAAGTACGTACCCAAAAAAAAGAAAGATGGGGAAGACTATGTTTGCTCCTGCTTTGGAAGAATTGGAGAGTGTCAAGTCTGAATCAAAAGAGATTCTGACGAAGCATTTCTTGGAATCGTGCATTAAGATTTTGCCTGTTCTAGCTGAATTTGGAACTGCTGTGTATCCTGTTACTTTTGACATCCGTACTTGTGTAGTGTATGGATATTCATCAGATCCTTCCAAGTTCAACTACTCGTTCAGTTTGGTGCAATTTGAGACCTTGCCTTCCAAGTTCAATTACAGTTCGTATCCATTCGAGACCTTTTCTCTTGCTTTGCTGACAAGAAACATGGATTTTGTGGTAGAACTGTTTCGTAACTTGCTGGAGCATCCGGAGTGGTTTACGATTGAGGCTTGTAAAGATTCCTACGGCAAGACCCTGAAAATATGGCTTGAACGAAAGAAATGGTTTAATCCAAATCTTCCATTGAAAAGAGTCATACCGGAGCTTTCTACGGACAGAGAGAAGTTCATGGAGAAGATCGAAGGCAATGGTGATGTGATGGGTGATATCAAGAAATTTTGCTCTATCTTTACACCTCTAATTAAAGAGATTGACAAGTTTTTGGCTAGTTTGGGCTCGGATAGAATTAAGCTGCTTATTGATGGGGAGCGGGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GLTP

Protein Analysis

478

Amino Acids

54.57

Weight (kDa)

8.79

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLTP PF08718 76 - 191 3.2e-15 Glycolipid transfer protein (GLTP)
GLTP PF08718 287 - 400 6.2e-11 Glycolipid transfer protein (GLTP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024453)

Species Orthologous Gene IDs
malus_domestica MD05G1300600.v1.1 MD05G1302000.v1.1
pyrus_communis pycom05g27850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
Acc65I GGTACC 1 cut(s) 763
AccB1I GGYRCC 1 cut(s) 763
AccBSI CCGCTC 1 cut(s) 1423
AccII CGCG 1 cut(s) 603
AccIII TCCGGA 2 cut(s) 157, 1141
AciI CCGC 4 cut(s) 17, 23, 147, 1423
AclWI GGATC 2 cut(s) 168, 977
AcsI RAATTY 4 cut(s) 579, 665, 914, 1328
AfaI GTAC 6 cut(s) 383, 430, 765, 772, 776, 955
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 9 cut(s) 281, 298, 424, 622, 733, 997, 1045, 1205, 1240
AjuI GAANNNNNNNTTGG 4 cut(s) 567, 599, 860, 892
AloI GAACNNNNNNTCC 2 cut(s) 158, 190
AluBI AGCT 6 cut(s) 171, 481, 530, 911, 1258, 1405
AluI AGCT 6 cut(s) 171, 481, 530, 911, 1258, 1405
Alw26I GTCTC 3 cut(s) 648, 1019, 1061
AlwI GGATC 2 cut(s) 168, 977
Aor13HI TCCGGA 2 cut(s) 157, 1141
AoxI GGCC 1 cut(s) 29
ApeKI GCWGC 2 cut(s) 20, 1405
ApoI RAATTY 4 cut(s) 579, 665, 914, 1328
Asp700I GAANNNNTTC 3 cut(s) 579, 872, 1119
Asp718I GGTACC 1 cut(s) 763
AspS9I GGNCC 4 cut(s) 29, 149, 210, 231
AsuHPI GGTGA 6 cut(s) 403, 445, 637, 653, 1316, 1328
AsuII TTCGAA 1 cut(s) 372
AsuNHI GCTAGC 1 cut(s) 746
AvaII GGWCC 3 cut(s) 149, 210, 231
BanI GGYRCC 1 cut(s) 763
BanII GRGCYC 1 cut(s) 1391
BauI CACGAG 1 cut(s) 704
BbsI GAAGAC 1 cut(s) 806
BbvI GCAGC 2 cut(s) 32, 1392
BccI CCATC 4 cut(s) 323, 787, 1306, 1409
BceAI ACGGC 2 cut(s) 124, 1195
BciVI GTATCC 2 cut(s) 942, 1068
BcoDI GTCTC 3 cut(s) 648, 1019, 1061
BfaI CTAG 3 cut(s) 747, 908, 1380
BfuI GTATCC 2 cut(s) 942, 1068
BisI GCNGC 4 cut(s) 18, 21, 24, 1406
BlsI GCNGC 4 cut(s) 19, 22, 25, 1407
Bme18I GGWCC 3 cut(s) 149, 210, 231
BmgT120I GGNCC 4 cut(s) 29, 149, 210, 231
BmiI GGNNCC 2 cut(s) 212, 765
BmsI GCATC 2 cut(s) 511, 1147
BmtI GCTAGC 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 806
BpmI CTGGAG 3 cut(s) 492, 738, 1154
Bpu14I TTCGAA 1 cut(s) 372
BsaAI YACGTR 2 cut(s) 497, 774
BsaBI GATNNNNATC 1 cut(s) 976
BsaI GGTCTC 2 cut(s) 1019, 1061
BsaWI WCCGGW 3 cut(s) 157, 1141, 1252
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse3DI GCAATG 2 cut(s) 643, 1306
Bse8I GATNNNNATC 1 cut(s) 976
BseAI TCCGGA 2 cut(s) 157, 1141
BseGI GGATG 5 cut(s) 311, 502, 730, 948, 1138
BseJI GATNNNNATC 1 cut(s) 976
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 2 cut(s) 643, 1306
BseRI GAGGAG 1 cut(s) 675
BseXI GCAGC 2 cut(s) 32, 1392
BsgI GTGCAG 3 cut(s) 249, 274, 348
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 1 cut(s) 31
BshNI GGYRCC 1 cut(s) 763
BsiSI CCGG 3 cut(s) 158, 1142, 1253
BslFI GGGAC 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 648, 1019, 1061
BsmFI GGGAC 1 cut(s) 196
BsnI GGCC 1 cut(s) 31
Bso31I GGTCTC 2 cut(s) 1019, 1061
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 1391
Bsp13I TCCGGA 2 cut(s) 157, 1141
Bsp1407I TGTACA 1 cut(s) 381
Bsp143I GATC 4 cut(s) 160, 627, 982, 1290
Bsp68I TCGCGA 1 cut(s) 603
BspACI CCGC 4 cut(s) 17, 23, 147, 1423
BspANI GGCC 1 cut(s) 31
BspEI TCCGGA 2 cut(s) 157, 1141
BspFNI CGCG 1 cut(s) 603
BspLI GGNNCC 2 cut(s) 212, 765
BspOI GCTAGC 1 cut(s) 750
BspPI GGATC 2 cut(s) 168, 977
BspT104I TTCGAA 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 763
BspTNI GGTCTC 2 cut(s) 1019, 1061
BsrBI CCGCTC 1 cut(s) 1423
BsrDI GCAATG 2 cut(s) 643, 1306
BsrGI TGTACA 1 cut(s) 381
BssMI GATC 4 cut(s) 160, 627, 982, 1290
BssSI CACGAG 1 cut(s) 704
Bst2BI CACGAG 1 cut(s) 704
Bst4CI ACNGT 3 cut(s) 386, 1052, 1119
BstAUI TGTACA 1 cut(s) 381
BstBAI YACGTR 2 cut(s) 497, 774
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 5 cut(s) 253, 257, 601, 748, 1425
BstF5I GGATG 5 cut(s) 311, 502, 730, 948, 1138
BstFNI CGCG 1 cut(s) 603
BstKTI GATC 4 cut(s) 163, 630, 985, 1293
BstMAI GTCTC 3 cut(s) 648, 1019, 1061
BstMBI GATC 4 cut(s) 160, 627, 982, 1290
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstSNI TACGTA 1 cut(s) 774
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 2 cut(s) 32, 1392
BstV2I GAAGAC 1 cut(s) 806
BstX2I RGATCY 1 cut(s) 982
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 1 cut(s) 982
BsuI GTATCC 2 cut(s) 942, 1068
BsuRI GGCC 1 cut(s) 31
BtsCI GGATG 5 cut(s) 311, 502, 730, 948, 1138
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 336
BtuMI TCGCGA 1 cut(s) 603
Cac8I GCNNGC 5 cut(s) 253, 257, 601, 748, 1425
Cfr13I GGNCC 4 cut(s) 29, 149, 210, 231
Csp6I GTAC 6 cut(s) 382, 429, 764, 771, 775, 954
CviAII CATG 9 cut(s) 90, 302, 398, 463, 569, 649, 658, 1099, 1282
CviQI GTAC 6 cut(s) 382, 429, 764, 771, 775, 954
DpnI GATC 4 cut(s) 162, 629, 984, 1292
DpnII GATC 4 cut(s) 160, 627, 982, 1290
Eco105I TACGTA 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 1391
Eco31I GGTCTC 2 cut(s) 1019, 1061
Eco32I GATATC 1 cut(s) 1321
Eco47I GGWCC 3 cut(s) 149, 210, 231
EcoO109I RGGNCCY 1 cut(s) 210
EcoRV GATATC 1 cut(s) 1321
EcoT38I GRGCYC 1 cut(s) 1391
FaeI CATG 9 cut(s) 93, 305, 401, 466, 572, 652, 661, 1102, 1285
FalI AAGNNNNNCTT 2 cut(s) 860, 892
FaqI GGGAC 1 cut(s) 196
FatI CATG 9 cut(s) 89, 301, 397, 462, 568, 648, 657, 1098, 1281
FauI CCCGC 1 cut(s) 1416
Fnu4HI GCNGC 4 cut(s) 18, 21, 24, 1406
FokI GGATG 5 cut(s) 298, 489, 737, 935, 1125
FriOI GRGCYC 1 cut(s) 1391
Fsp4HI GCNGC 4 cut(s) 18, 21, 24, 1406
FspBI CTAG 3 cut(s) 747, 908, 1380
GluI GCNGC 4 cut(s) 18, 21, 24, 1406
GsuI CTGGAG 3 cut(s) 492, 738, 1154
HaeIII GGCC 1 cut(s) 31
HapII CCGG 3 cut(s) 158, 1142, 1253
Hin1II CATG 9 cut(s) 93, 305, 401, 466, 572, 652, 661, 1102, 1285
HpaII CCGG 3 cut(s) 158, 1142, 1253
HphI GGTGA 6 cut(s) 403, 445, 637, 653, 1316, 1328
Hpy166II GTNNAC 3 cut(s) 177, 394, 1152
Hpy188I TCNGA 7 cut(s) 231, 489, 632, 847, 864, 982, 1393
Hpy188III TCNNGA 9 cut(s) 158, 164, 509, 602, 704, 1066, 1142, 1324, 1431
Hpy8I GTNNAC 3 cut(s) 177, 394, 1152
HpyAV CCTTC 5 cut(s) 372, 616, 996, 1044, 1289
HpyCH4III ACNGT 3 cut(s) 386, 1052, 1119
HpyCH4IV ACGT 3 cut(s) 75, 496, 773
HpyCH4V TGCA 6 cut(s) 255, 266, 329, 672, 888, 1018
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpySE526I ACGT 3 cut(s) 75, 496, 773
Hsp92II CATG 9 cut(s) 93, 305, 401, 466, 572, 652, 661, 1102, 1285
Kpn2I TCCGGA 2 cut(s) 157, 1141
KpnI GGTACC 1 cut(s) 767
Kzo9I GATC 4 cut(s) 160, 627, 982, 1290
LmnI GCTCC 5 cut(s) 160, 817, 1135, 1255, 1420
Lsp1109I GCAGC 2 cut(s) 32, 1392
LweI GCATC 2 cut(s) 511, 1147
MaeI CTAG 3 cut(s) 747, 908, 1380
MaeII ACGT 3 cut(s) 75, 496, 773
MaeIII GTNAC 3 cut(s) 517, 937, 1124
MalI GATC 4 cut(s) 162, 629, 984, 1292
MbiI CCGCTC 1 cut(s) 1423
MboI GATC 4 cut(s) 160, 627, 982, 1290
MboII GAAGA 4 cut(s) 811, 836, 1223, 1300
MflI RGATCY 1 cut(s) 982
MhlI GDGCHC 1 cut(s) 1391
MlyI GAGTC 1 cut(s) 1254
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 6 cut(s) 442, 516, 626, 696, 1153, 1359
MroI TCCGGA 2 cut(s) 157, 1141
MroXI GAANNNNTTC 3 cut(s) 579, 872, 1119
MseI TTAA 5 cut(s) 693, 891, 1221, 1356, 1401
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 158, 1142, 1253
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 4 cut(s) 160, 627, 982, 1290
NheI GCTAGC 1 cut(s) 746
NlaIII CATG 9 cut(s) 93, 305, 401, 466, 572, 652, 661, 1102, 1285
NlaIV GGNNCC 2 cut(s) 212, 765
NruI TCGCGA 1 cut(s) 603
NspV TTCGAA 1 cut(s) 372
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PdmI GAANNNNTTC 3 cut(s) 579, 872, 1119
PfeI GAWTC 9 cut(s) 107, 226, 298, 523, 700, 848, 859, 881, 1172
PkrI GCNGC 4 cut(s) 19, 22, 25, 1407
PleI GAGTC 1 cut(s) 1253
PpsI GAGTC 1 cut(s) 1253
Ppu21I YACGTR 2 cut(s) 497, 774
PpuMI RGGWCCY 1 cut(s) 210
PsiI TTATAA 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 212, 765
PspPI GGNCC 4 cut(s) 29, 149, 210, 231
PspPPI RGGWCCY 1 cut(s) 210
PsuI RGATCY 1 cut(s) 982
RruI TCGCGA 1 cut(s) 603
RsaI GTAC 6 cut(s) 383, 430, 765, 772, 776, 955
RsaNI GTAC 6 cut(s) 382, 429, 764, 771, 775, 954
SaqAI TTAA 5 cut(s) 693, 891, 1221, 1356, 1401
SatI GCNGC 4 cut(s) 18, 21, 24, 1406
Sau3AI GATC 4 cut(s) 160, 627, 982, 1290
Sau96I GGNCC 4 cut(s) 29, 149, 210, 231
SchI GAGTC 1 cut(s) 1254
SduI GDGCHC 1 cut(s) 1391
SfaNI GCATC 2 cut(s) 511, 1147
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 3 cut(s) 149, 210, 231
SnaBI TACGTA 1 cut(s) 774
SsiI CCGC 4 cut(s) 17, 23, 147, 1423
SspMI CTAG 3 cut(s) 747, 908, 1380
TaaI ACNGT 3 cut(s) 386, 1052, 1119
TaiI ACGT 3 cut(s) 78, 499, 776
TaqI TCGA 4 cut(s) 372, 526, 1065, 1293
TatI WGTACW 1 cut(s) 381
TauI GCSGC 2 cut(s) 20, 26
TfiI GAWTC 9 cut(s) 107, 226, 298, 523, 700, 848, 859, 881, 1172
Tru1I TTAA 5 cut(s) 693, 891, 1221, 1356, 1401
Tru9I TTAA 5 cut(s) 693, 891, 1221, 1356, 1401
TscAI CASTG 1 cut(s) 336
TseI GCWGC 2 cut(s) 20, 1405
TspDTI ATGAA 3 cut(s) 588, 966, 1270
TspGWI ACGGA 3 cut(s) 424, 941, 1280
TspRI CASTG 1 cut(s) 336
VpaK11BI GGWCC 3 cut(s) 149, 210, 231
XapI RAATTY 4 cut(s) 579, 665, 914, 1328
XmnI GAANNNNTTC 3 cut(s) 579, 872, 1119
XspI CTAG 3 cut(s) 747, 908, 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.