MD05G1302000.v1.1

Pleckstrin homology domain-containing family A member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0024453
Physical Location & Seq
Forward (+)
43452496 .. 43458094
5599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1302000.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGTCAAAGAGAAAAGCGGCAGGGCCAATAAAAGAGAGTTCGCAGACAACCAAAGTAAAAGAAACAGACGCCCTTACGGCATGGAAAAGGAAACAGGAATCCATTGTGGCAAATTATGCTATTCTCTGGCCGTTGGGCGGTCCTGCTCCGGATCGGGAGTGCCTTTTCGCTCCTGTTTTGGAAATGTTGGAGTTTGTCAGGTCCCCAAGTGGAGAGATTCGGACCGTCCCTTTCTTGGTAGTGTGCAGGCATACTGCACCAATTACAATTCAATTTCAAGACCCTTTGAATCATGTTAGGACGTCCATCTTTTCTTGTGCAGTGAAAATGGAAAGAATATATCAATCCAATCCTTCCAAGTTCGAACACTTGTACAGTTTGGTGAACTTTGATGTCGAAACAAATAATTCTGATGAACAATGCTCGAGTACCGTTTCTCTTGTTTGGCTCACAAGATACATGGATTTTTTGGTAGAGCTGTTTCGGAACTTACGGGAGCATCCTCCAGATTGGGTTACAATTCGGGCTGTTTATAACGCCTATGCGAAGACCCTGATAAAATGGCATGATGAAAAAATTCTTGGACGCAAGTTTGCTCCTGAAAAAGACTTGTTGTTGAAGGTGATCGGAGGCAATGGTGATGTCATGGGAGACATGGAGAAATTTTGCACTTCCTTTACTCCTCTACTTAAACAGAATCACGAGTTTTTGGCTAGTCTTGGAATGGATGATTTGAAGTTATCTGGTGCTAGCCTTGTAGCAAAGGTACCAAGTACATACCCAAAAAAAGAAAAGATGGGGAAGACTATGTTTGCTCCTGCTTTGGAAGAATTGGAGAGCGTGAAGTCTAAATCAAAAGAGATTCTGACGAAGCATTTCTTGGAATCGTGCAATAAGATTTTGCCTGTTATAGCTAAATTTGGAAATGCCGTGTATTCTGTTAATTATACCATCCGTCTTCATGTAGCGAGATTGCAGTCTAGATACTCATCAGATCCTTCCAAGTTCAACTACTTGTACGGTTTCATGCAATTTGAGATTGAAACATTTTCTCTTAATATTCTGACAAGAAACATGGATTTTGTGGTAGAACTGTTTCGGAACTTGCTGGAGCATCCGGAGTGGTTTACGATTGAGGCTTGTAAAGATGCCTATGGCAAGACCCTGAAAATATGGTTTGAACGACAGGAATGGTTTGAACCAAATAATACATTGAGTAAAGTCTACCCGGCGCTTTCTACGGACAGAGAAAAGTTCATGGAGGCAATCGAAGGCAATGGTGATGTGATGGGTGGTATAAAGAAATTTTGCTCTATCTTTACACCTCTAATTAAAGAGATTGACAAGTTTTTGGCTAGTTCGGGCTCGGATAAATCTAAGCTACTTATTGATGGGGGGGGAGGTTTCTTGATTCAGTTGCATTGCCTGCCATTTGCCTCCCGTTATTGCGGATCTTCGGATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GLTP

Protein Analysis

489

Amino Acids

55.58

Weight (kDa)

8.33

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLTP PF08718 73 - 190 5.4e-15 Glycolipid transfer protein (GLTP)
GLTP PF08718 288 - 392 2.7e-14 Glycolipid transfer protein (GLTP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024453)

Species Orthologous Gene IDs
malus_domestica MD05G1300600.v1.1 MD05G1302000.v1.1
pyrus_communis pycom05g27850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
AatII GACGTC 1 cut(s) 305
Acc65I GGTACC 1 cut(s) 766
AccB1I GGYRCC 1 cut(s) 766
AccI GTMKAC 1 cut(s) 1224
AccIII TCCGGA 2 cut(s) 148, 1117
AciI CCGC 3 cut(s) 17, 138, 1449
AclWI GGATC 3 cut(s) 159, 989, 1459
AcoI YGGCCR 1 cut(s) 128
AcsI RAATTY 4 cut(s) 576, 662, 917, 1304
AcyI GRCGYC 2 cut(s) 69, 302
AfaI GTAC 5 cut(s) 374, 430, 768, 775, 1019
AfiI CCNNNNNNNGG 2 cut(s) 137, 235
AgsI TTSAA 9 cut(s) 272, 278, 289, 619, 736, 1009, 1043, 1181, 1199
AjuI GAANNNNNNNTTGG 4 cut(s) 564, 596, 863, 895
AluBI AGCT 3 cut(s) 478, 914, 1381
AluI AGCT 3 cut(s) 478, 914, 1381
Alw26I GTCTC 1 cut(s) 645
AlwI GGATC 3 cut(s) 159, 989, 1459
Ama87I CYCGRG 1 cut(s) 424
Aor13HI TCCGGA 2 cut(s) 148, 1117
AoxI GGCC 2 cut(s) 23, 128
ApoI RAATTY 4 cut(s) 576, 662, 917, 1304
Asp700I GAANNNNTTC 3 cut(s) 576, 875, 1095
Asp718I GGTACC 1 cut(s) 766
AspLEI GCGC 1 cut(s) 1234
AspS9I GGNCC 4 cut(s) 23, 140, 201, 222
AsuC2I CCSGG 1 cut(s) 1229
AsuHPI GGTGA 4 cut(s) 394, 634, 650, 1292
AsuII TTCGAA 1 cut(s) 363
AsuNHI GCTAGC 1 cut(s) 749
AvaI CYCGRG 1 cut(s) 424
AvaII GGWCC 3 cut(s) 140, 201, 222
BanI GGYRCC 1 cut(s) 766
BanII GRGCYC 1 cut(s) 1367
BauI CACGAG 1 cut(s) 701
BbsI GAAGAC 3 cut(s) 554, 809, 950
BccI CCATC 5 cut(s) 314, 790, 959, 1282, 1385
BceAI ACGGC 3 cut(s) 93, 115, 914
BcnI CCSGG 1 cut(s) 1229
BcoDI GTCTC 1 cut(s) 645
BfaI CTAG 4 cut(s) 714, 750, 981, 1356
BfoI RGCGCY 1 cut(s) 1235
BglI GCCNNNNNGGC 1 cut(s) 77
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 1229
Bme18I GGWCC 3 cut(s) 140, 201, 222
BmeT110I CYCGRG 1 cut(s) 424
BmgT120I GGNCC 4 cut(s) 23, 140, 201, 222
BmiI GGNNCC 2 cut(s) 203, 768
BmrFI CCNGG 1 cut(s) 1229
BmsI GCATC 3 cut(s) 508, 1123, 1138
BmtI GCTAGC 1 cut(s) 753
BpiI GAAGAC 3 cut(s) 554, 809, 950
BpmI CTGGAG 2 cut(s) 489, 1130
Bpu14I TTCGAA 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 1229
BsaBI GATNNNNATC 1 cut(s) 988
BsaHI GRCGYC 2 cut(s) 69, 302
BsaWI WCCGGW 2 cut(s) 148, 1117
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 235
Bse3DI GCAATG 3 cut(s) 640, 1282, 1420
Bse8I GATNNNNATC 1 cut(s) 988
BseAI TCCGGA 2 cut(s) 148, 1117
BseGI GGATG 4 cut(s) 499, 733, 951, 1114
BseJI GATNNNNATC 1 cut(s) 988
BseLI CCNNNNNNNGG 2 cut(s) 137, 235
BseMI GCAATG 3 cut(s) 640, 1282, 1420
BseRI GAGGAG 1 cut(s) 672
BsgI GTGCAG 3 cut(s) 240, 265, 339
BshFI GGCC 2 cut(s) 25, 130
BshNI GGYRCC 1 cut(s) 766
BsiHKCI CYCGRG 1 cut(s) 424
BsiSI CCGG 3 cut(s) 149, 1118, 1229
BslFI GGGAC 2 cut(s) 187, 212
BslI CCNNNNNNNGG 2 cut(s) 137, 235
BsmAI GTCTC 1 cut(s) 645
BsmFI GGGAC 2 cut(s) 187, 212
BsnI GGCC 2 cut(s) 25, 130
BsoBI CYCGRG 1 cut(s) 424
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 1 cut(s) 1367
Bsp13I TCCGGA 2 cut(s) 148, 1117
Bsp1407I TGTACA 1 cut(s) 372
Bsp143I GATC 4 cut(s) 151, 624, 994, 1451
BspACI CCGC 3 cut(s) 17, 138, 1449
BspANI GGCC 2 cut(s) 25, 130
BspEI TCCGGA 2 cut(s) 148, 1117
BspLI GGNNCC 2 cut(s) 203, 768
BspOI GCTAGC 1 cut(s) 753
BspPI GGATC 3 cut(s) 159, 989, 1459
BspT104I TTCGAA 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 766
BsrDI GCAATG 3 cut(s) 640, 1282, 1420
BsrGI TGTACA 1 cut(s) 372
BssMI GATC 4 cut(s) 151, 624, 994, 1451
BssNI GRCGYC 2 cut(s) 69, 302
BssSI CACGAG 1 cut(s) 701
Bst2BI CACGAG 1 cut(s) 701
Bst4CI ACNGT 5 cut(s) 226, 377, 433, 1022, 1095
BstACI GRCGYC 2 cut(s) 69, 302
BstAPI GCANNNNNTGC 2 cut(s) 116, 1426
BstAUI TGTACA 1 cut(s) 372
BstBI TTCGAA 1 cut(s) 363
BstC8I GCNNGC 3 cut(s) 248, 751, 1427
BstDEI CTNAG 1 cut(s) 1377
BstF5I GGATG 4 cut(s) 499, 733, 951, 1114
BstH2I RGCGCY 1 cut(s) 1235
BstHHI GCGC 1 cut(s) 1234
BstKTI GATC 4 cut(s) 154, 627, 997, 1454
BstMAI GTCTC 1 cut(s) 645
BstMBI GATC 4 cut(s) 151, 624, 994, 1451
BstMWI GCNNNNNNNGC 3 cut(s) 77, 116, 1426
BstSCI CCNGG 1 cut(s) 1227
BstV2I GAAGAC 3 cut(s) 554, 809, 950
BstX2I RGATCY 2 cut(s) 994, 1451
BstYI RGATCY 2 cut(s) 994, 1451
BsuRI GGCC 2 cut(s) 25, 130
BtsCI GGATG 4 cut(s) 499, 733, 951, 1114
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 327
Cac8I GCNNGC 3 cut(s) 248, 751, 1427
CfoI GCGC 1 cut(s) 1234
Cfr13I GGNCC 4 cut(s) 23, 140, 201, 222
CpoI CGGWCCG 1 cut(s) 222
CseI GACGC 2 cut(s) 77, 594
Csp6I GTAC 5 cut(s) 373, 429, 767, 774, 1018
CspI CGGWCCG 1 cut(s) 222
CviQI GTAC 5 cut(s) 373, 429, 767, 774, 1018
DdeI CTNAG 1 cut(s) 1377
DpnI GATC 4 cut(s) 153, 626, 996, 1453
DpnII GATC 4 cut(s) 151, 624, 994, 1451
EaeI YGGCCR 1 cut(s) 128
Eco24I GRGCYC 1 cut(s) 1367
Eco47I GGWCC 3 cut(s) 140, 201, 222
Eco88I CYCGRG 1 cut(s) 424
EcoO109I RGGNCCY 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 1367
FalI AAGNNNNNCTT 2 cut(s) 863, 895
FaqI GGGAC 2 cut(s) 187, 212
FblI GTMKAC 1 cut(s) 1224
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 4 cut(s) 486, 740, 938, 1101
FriOI GRGCYC 1 cut(s) 1367
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 4 cut(s) 714, 750, 981, 1356
GlaI GCGC 1 cut(s) 1233
GluI GCNGC 1 cut(s) 18
GsuI CTGGAG 2 cut(s) 489, 1130
HaeII RGCGCY 1 cut(s) 1235
HaeIII GGCC 2 cut(s) 25, 130
HapII CCGG 3 cut(s) 149, 1118, 1229
HgaI GACGC 2 cut(s) 77, 594
HhaI GCGC 1 cut(s) 1234
Hin1I GRCGYC 2 cut(s) 69, 302
Hin6I GCGC 1 cut(s) 1232
HinP1I GCGC 1 cut(s) 1232
HinfI GANTC 7 cut(s) 98, 217, 289, 697, 862, 884, 1411
HpaII CCGG 3 cut(s) 149, 1118, 1229
HphI GGTGA 4 cut(s) 394, 634, 650, 1292
Hpy166II GTNNAC 3 cut(s) 385, 1128, 1225
Hpy188III TCNNGA 9 cut(s) 149, 155, 278, 506, 599, 701, 981, 1118, 1408
Hpy8I GTNNAC 3 cut(s) 385, 1128, 1225
HpyAV CCTTC 4 cut(s) 363, 613, 1008, 1265
HpyCH4III ACNGT 5 cut(s) 226, 377, 433, 1022, 1095
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 8 cut(s) 246, 257, 320, 669, 891, 976, 1030, 1420
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 116, 1426
HpyF3I CTNAG 1 cut(s) 1377
HpySE526I ACGT 1 cut(s) 302
Hsp92I GRCGYC 2 cut(s) 69, 302
HspAI GCGC 1 cut(s) 1232
Kpn2I TCCGGA 2 cut(s) 148, 1117
KpnI GGTACC 1 cut(s) 770
Kzo9I GATC 4 cut(s) 151, 624, 994, 1451
LmnI GCTCC 6 cut(s) 151, 175, 496, 601, 820, 1111
LweI GCATC 3 cut(s) 508, 1123, 1138
MaeI CTAG 4 cut(s) 714, 750, 981, 1356
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 514
MalI GATC 4 cut(s) 153, 626, 996, 1453
MboI GATC 4 cut(s) 151, 624, 994, 1451
MboII GAAGA 5 cut(s) 559, 814, 839, 950, 1446
MflI RGATCY 2 cut(s) 994, 1451
MhlI GDGCHC 1 cut(s) 1367
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 8 cut(s) 513, 623, 693, 1129, 1255, 1335, 1394, 1447
MroI TCCGGA 2 cut(s) 148, 1117
MroXI GAANNNNTTC 3 cut(s) 576, 875, 1095
MseI TTAA 4 cut(s) 690, 942, 1056, 1332
MspI CCGG 3 cut(s) 149, 1118, 1229
MspR9I CCNGG 1 cut(s) 1229
MwoI GCNNNNNNNGC 3 cut(s) 77, 116, 1426
NciI CCSGG 1 cut(s) 1229
NdeII GATC 4 cut(s) 151, 624, 994, 1451
NheI GCTAGC 1 cut(s) 749
NlaIV GGNNCC 2 cut(s) 203, 768
NspV TTCGAA 1 cut(s) 363
PaeR7I CTCGAG 1 cut(s) 424
PdmI GAANNNNTTC 3 cut(s) 576, 875, 1095
PfeI GAWTC 7 cut(s) 98, 217, 289, 697, 862, 884, 1411
PkrI GCNGC 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 201
PsiI TTATAA 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 203, 768
PspPI GGNCC 4 cut(s) 23, 140, 201, 222
PspPPI RGGWCCY 1 cut(s) 201
PspXI VCTCGAGB 1 cut(s) 424
PsuI RGATCY 2 cut(s) 994, 1451
RsaI GTAC 5 cut(s) 374, 430, 768, 775, 1019
RsaNI GTAC 5 cut(s) 373, 429, 767, 774, 1018
Rsr2I CGGWCCG 1 cut(s) 222
RsrII CGGWCCG 1 cut(s) 222
SaqAI TTAA 4 cut(s) 690, 942, 1056, 1332
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 4 cut(s) 151, 624, 994, 1451
Sau96I GGNCC 4 cut(s) 23, 140, 201, 222
ScrFI CCNGG 1 cut(s) 1229
SduI GDGCHC 1 cut(s) 1367
SetI ASST 9 cut(s) 203, 305, 480, 624, 768, 916, 1327, 1383, 1405
SfaNI GCATC 3 cut(s) 508, 1123, 1138
Sfr274I CTCGAG 1 cut(s) 424
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 3 cut(s) 140, 201, 222
SlaI CTCGAG 1 cut(s) 424
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SsiI CCGC 3 cut(s) 17, 138, 1449
SspI AATATT 1 cut(s) 1060
SspMI CTAG 4 cut(s) 714, 750, 981, 1356
StyD4I CCNGG 1 cut(s) 1227
TaaI ACNGT 5 cut(s) 226, 377, 433, 1022, 1095
TaiI ACGT 1 cut(s) 305
TaqI TCGA 4 cut(s) 363, 396, 425, 1269
TatI WGTACW 2 cut(s) 372, 773
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 7 cut(s) 98, 217, 289, 697, 862, 884, 1411
Tru1I TTAA 4 cut(s) 690, 942, 1056, 1332
Tru9I TTAA 4 cut(s) 690, 942, 1056, 1332
TscAI CASTG 1 cut(s) 327
TspDTI ATGAA 5 cut(s) 429, 585, 950, 1015, 1246
TspGWI ACGGA 2 cut(s) 944, 1256
TspRI CASTG 1 cut(s) 327
VpaK11BI GGWCC 3 cut(s) 140, 201, 222
XapI RAATTY 4 cut(s) 576, 662, 917, 1304
XbaI TCTAGA 1 cut(s) 980
XhoI CTCGAG 1 cut(s) 424
XmiI GTMKAC 1 cut(s) 1224
XmnI GAANNNNTTC 3 cut(s) 576, 875, 1095
XspI CTAG 4 cut(s) 714, 750, 981, 1356
ZraI GACGTC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.