MD05G1311200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0022181
Physical Location & Seq
Forward (+)
44128120 .. 44129067
948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1311200.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGTCTGCAATGATTGGAGGACTCGCTCAACACGGGCATGGAAGAGAGGCCCTCAACTTATTCACTCAGATGCTCGAGGATGGTGTTTCCCCCAATCACATTACTTTAGTAATGGTGTTTCCCCCAATCACATTACTTTAGTAAGTGTCCTCTGTGCCTGCAATCACCCAGGTTTGGTAATGGAAGCCAAAAAGTATTTCGAGTCCATGACAGAGTTGTTTGGAGTTGTGCCGAGGCAAGAGCATTATGCTTGCATGATTGATCTCCTTAGCAGAGCAGGGAAGATAAATGAGGCAACGGAGCTTGTCAACACAATGCCATTTCAAGCGAACACATCTGTTTGGGGGGCACTTCTTGGTGCTGCGAGAATCCATAAAAATGTTGAGCTTGGCCAGTGCGCTGCTGAAATGCTCTTAGTTCTTGAGCCCGAGAAATCTGGTACTCACGTACTTCTTGCAAACATTTGCACATCAGCCGGCATGTGGGGTAATGTGGCGAAAATGAGAAAACTTATGAAAGATGGCCAGGTAAAGAAGGAACCTGGGATGAGTTGGATTGAGGTCAGAGACCAGGTACACACATTTATAGTAGGAGATAGAAGCCATTCAAGAAGCGATGAAGTTTATGCGAAATTCGATGAACTTTTCAACCTACTGTACAAAGCCGGCTATGTTCCAATGGTGGAGATGGACCTCCATGACGTGGAACAAGGCGAAAAGCAGCGGCTTCTGCGCTACCACAGCGAGAAGCTTACAGTGGCTTTCGCGTTGATTGCCACTCGGCCGGGAGCTCCCATCAGGGTGAAGAAGAATCTAAGAGTACGTGTGGACTGCCATACAGCACTAAAGTTCATCTGTAAGATTGTTTCGAGGGAGATTATTGTGAGGGACGTCAACAGATTCCATCATTTCAAAGATGGCTCATGCTCTTGTGGGGATTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

316

Amino Acids

35.79

Weight (kDa)

8.91

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 125 - 187 4.6e-19 E motif
DYW_deaminase PF14432 223 - 315 1.2e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022181)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08730
malus_domestica MD05G1311200.v1.1
pyrus_communis pycom14g06810
rosa_multiflora Rmu_sc0025557.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 894
AccB7I CCANNNNNTGG 1 cut(s) 703
AccII CGCG 1 cut(s) 767
AciI CCGC 1 cut(s) 724
AcoI YGGCCR 3 cut(s) 391, 523, 782
AcsI RAATTY 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 891
AfaI GTAC 5 cut(s) 442, 450, 576, 659, 822
AfiI CCNNNNNNNGG 3 cut(s) 175, 483, 703
AflIII ACRYGT 1 cut(s) 823
AgsI TTSAA 4 cut(s) 326, 609, 649, 913
AjiI CACGTC 1 cut(s) 703
AjnI CCWGG 4 cut(s) 169, 525, 541, 570
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 4 cut(s) 304, 388, 751, 791
AluI AGCT 4 cut(s) 304, 388, 751, 791
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 1 cut(s) 561
Ama87I CYCGRG 2 cut(s) 75, 428
AoxI GGCC 4 cut(s) 49, 391, 523, 782
ApeKI GCWGC 3 cut(s) 362, 401, 721
ApoI RAATTY 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 2 cut(s) 401, 735
AspS9I GGNCC 2 cut(s) 50, 691
AsuC2I CCSGG 1 cut(s) 786
AsuHPI GGTGA 2 cut(s) 158, 814
AvaI CYCGRG 2 cut(s) 75, 428
AvaII GGWCC 1 cut(s) 691
BaeGI GKGCMC 1 cut(s) 352
BaeI ACNNNNGTAYC 2 cut(s) 432, 465
BalI TGGCCA 2 cut(s) 393, 525
BanII GRGCYC 2 cut(s) 429, 793
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 3 cut(s) 349, 388, 733
BccI CCATC 6 cut(s) 75, 515, 682, 803, 911, 912
BciT130I CCWGG 4 cut(s) 171, 527, 543, 572
BcnI CCSGG 1 cut(s) 786
BcoDI GTCTC 1 cut(s) 561
BisI GCNGC 4 cut(s) 363, 402, 722, 725
BlsI GCNGC 4 cut(s) 364, 403, 723, 726
Bme1390I CCNGG 5 cut(s) 171, 527, 543, 572, 786
Bme18I GGWCC 1 cut(s) 691
BmeT110I CYCGRG 2 cut(s) 75, 428
BmgBI CACGTC 1 cut(s) 703
BmgT120I GGNCC 2 cut(s) 50, 691
BmiI GGNNCC 1 cut(s) 540
BmrFI CCNGG 5 cut(s) 171, 527, 543, 572, 786
BmsI GCATC 1 cut(s) 61
BplI GAGNNNNNCTC 2 cut(s) 37, 69
Bpu10I CCTNAGC 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 786
BsaAI YACGTR 2 cut(s) 448, 824
BsaHI GRCGYC 1 cut(s) 891
BsaI GGTCTC 1 cut(s) 561
BsaJI CCNNGG 3 cut(s) 169, 233, 542
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 483, 703
Bse118I RCCGGY 2 cut(s) 476, 665
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 16
BseBI CCWGG 4 cut(s) 171, 527, 543, 572
BseDI CCNNGG 3 cut(s) 169, 233, 542
BseGI GGATG 2 cut(s) 86, 552
BseLI CCNNNNNNNGG 3 cut(s) 175, 483, 703
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 1 cut(s) 394
BseSI GKGCMC 1 cut(s) 352
BseX3I CGGCCG 1 cut(s) 782
BseXI GCAGC 3 cut(s) 349, 388, 733
Bsh1236I CGCG 1 cut(s) 767
Bsh1285I CGRYCG 1 cut(s) 785
BshFI GGCC 4 cut(s) 51, 393, 525, 784
BsiEI CGRYCG 1 cut(s) 785
BsiHKAI GWGCWC 1 cut(s) 793
BsiHKCI CYCGRG 2 cut(s) 75, 428
BsiSI CCGG 3 cut(s) 477, 666, 785
BslFI GGGAC 1 cut(s) 902
BslI CCNNNNNNNGG 3 cut(s) 175, 483, 703
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 1 cut(s) 902
BsnI GGCC 4 cut(s) 51, 393, 525, 784
Bso31I GGTCTC 1 cut(s) 561
BsoBI CYCGRG 2 cut(s) 75, 428
Bsp1286I GDGCHC 3 cut(s) 352, 429, 793
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 1 cut(s) 262
BspACI CCGC 1 cut(s) 724
BspANI GGCC 4 cut(s) 51, 393, 525, 784
BspCNI CTCAG 1 cut(s) 80
BspFNI CGCG 1 cut(s) 767
BspLI GGNNCC 1 cut(s) 540
BspTNI GGTCTC 1 cut(s) 561
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 2 cut(s) 476, 665
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 2 cut(s) 476, 665
BssECI CCNNGG 3 cut(s) 169, 233, 542
BssMI GATC 1 cut(s) 262
BssNI GRCGYC 1 cut(s) 891
Bst2UI CCWGG 4 cut(s) 171, 527, 543, 572
Bst4CI ACNGT 2 cut(s) 657, 757
Bst6I CTCTTC 1 cut(s) 38
BstACI GRCGYC 1 cut(s) 891
BstAUI TGTACA 1 cut(s) 657
BstBAI YACGTR 2 cut(s) 448, 824
BstC8I GCNNGC 4 cut(s) 160, 253, 478, 667
BstDEI CTNAG 4 cut(s) 67, 269, 415, 815
BstF5I GGATG 2 cut(s) 86, 552
BstFNI CGCG 1 cut(s) 767
BstHHI GCGC 2 cut(s) 401, 735
BstKTI GATC 1 cut(s) 265
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 1 cut(s) 262
BstMCI CGRYCG 1 cut(s) 785
BstMWI GCNNNNNNNGC 3 cut(s) 730, 741, 773
BstNI CCWGG 4 cut(s) 171, 527, 543, 572
BstNSI RCATGY 1 cut(s) 484
BstSCI CCNGG 5 cut(s) 169, 525, 541, 570, 784
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 1 cut(s) 767
BstV1I GCAGC 3 cut(s) 349, 388, 733
BstZI CGGCCG 1 cut(s) 782
BsuRI GGCC 4 cut(s) 51, 393, 525, 784
BtgZI GCGATG 1 cut(s) 630
BtrI CACGTC 1 cut(s) 703
BtsCI GGATG 2 cut(s) 86, 552
BtsIMutI CAGTG 2 cut(s) 401, 762
Cac8I GCNNGC 4 cut(s) 160, 253, 478, 667
CfoI GCGC 2 cut(s) 401, 735
Cfr10I RCCGGY 2 cut(s) 476, 665
Cfr13I GGNCC 2 cut(s) 50, 691
CsiI ACCWGGT 1 cut(s) 570
Csp6I GTAC 5 cut(s) 441, 449, 575, 658, 821
CviAII CATG 6 cut(s) 39, 208, 256, 481, 698, 924
CviQI GTAC 5 cut(s) 441, 449, 575, 658, 821
DdeI CTNAG 4 cut(s) 67, 269, 415, 815
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
EaeI YGGCCR 3 cut(s) 391, 523, 782
EagI CGGCCG 1 cut(s) 782
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 791
EclXI CGGCCG 1 cut(s) 782
Eco24I GRGCYC 2 cut(s) 429, 793
Eco31I GGTCTC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 691
Eco52I CGGCCG 1 cut(s) 782
Eco53kI GAGCTC 1 cut(s) 791
Eco88I CYCGRG 2 cut(s) 75, 428
EcoICRI GAGCTC 1 cut(s) 791
EcoO109I RGGNCCY 1 cut(s) 50
EcoRII CCWGG 4 cut(s) 169, 525, 541, 570
EcoT38I GRGCYC 2 cut(s) 429, 793
FaeI CATG 6 cut(s) 42, 211, 259, 484, 701, 927
FaqI GGGAC 1 cut(s) 902
FatI CATG 6 cut(s) 38, 207, 255, 480, 697, 923
Fnu4HI GCNGC 4 cut(s) 363, 402, 722, 725
FokI GGATG 2 cut(s) 93, 559
FriOI GRGCYC 2 cut(s) 429, 793
Fsp4HI GCNGC 4 cut(s) 363, 402, 722, 725
GlaI GCGC 2 cut(s) 400, 734
GluI GCNGC 4 cut(s) 363, 402, 722, 725
HaeIII GGCC 4 cut(s) 51, 393, 525, 784
HapII CCGG 3 cut(s) 477, 666, 785
HhaI GCGC 2 cut(s) 401, 735
Hin1I GRCGYC 1 cut(s) 891
Hin1II CATG 6 cut(s) 42, 211, 259, 484, 701, 927
Hin6I GCGC 2 cut(s) 399, 733
HinP1I GCGC 2 cut(s) 399, 733
HincII GTYRAC 2 cut(s) 310, 895
HindII GTYRAC 2 cut(s) 310, 895
HindIII AAGCTT 1 cut(s) 749
HinfI GANTC 5 cut(s) 22, 203, 369, 811, 900
HpaII CCGG 3 cut(s) 477, 666, 785
HphI GGTGA 2 cut(s) 158, 814
Hpy166II GTNNAC 4 cut(s) 310, 577, 829, 895
Hpy188I TCNGA 2 cut(s) 70, 566
Hpy188III TCNNGA 2 cut(s) 422, 609
Hpy8I GTNNAC 4 cut(s) 310, 577, 829, 895
HpyAV CCTTC 1 cut(s) 529
HpyCH4III ACNGT 2 cut(s) 657, 757
HpyCH4IV ACGT 4 cut(s) 447, 702, 823, 891
HpyCH4V TGCA 5 cut(s) 9, 162, 255, 458, 468
HpyF10VI GCNNNNNNNGC 3 cut(s) 730, 741, 773
HpyF3I CTNAG 4 cut(s) 67, 269, 415, 815
HpySE526I ACGT 4 cut(s) 447, 702, 823, 891
Hsp92I GRCGYC 1 cut(s) 891
Hsp92II CATG 6 cut(s) 42, 211, 259, 484, 701, 927
HspAI GCGC 2 cut(s) 399, 733
KroI GCCGGC 2 cut(s) 476, 665
KroNI GCCGGC 2 cut(s) 478, 667
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 3 cut(s) 301, 788, 796
Lsp1109I GCAGC 3 cut(s) 349, 388, 733
LweI GCATC 1 cut(s) 61
MabI ACCWGGT 1 cut(s) 570
MaeII ACGT 4 cut(s) 447, 702, 823, 891
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 4 cut(s) 55, 295, 817, 820
MhlI GDGCHC 3 cut(s) 352, 429, 793
MlsI TGGCCA 2 cut(s) 393, 525
MluCI AATT 1 cut(s) 632
MluNI TGGCCA 2 cut(s) 393, 525
MlyI GAGTC 2 cut(s) 16, 212
MmeI TCCRAC 1 cut(s) 533
Mox20I TGGCCA 2 cut(s) 393, 525
MroNI GCCGGC 2 cut(s) 476, 665
MroXI GAANNNNTTC 1 cut(s) 604
MscI TGGCCA 2 cut(s) 393, 525
MslI CAYNNNNRTG 4 cut(s) 37, 69, 378, 800
Msp20I TGGCCA 2 cut(s) 393, 525
MspA1I CMGCKG 1 cut(s) 724
MspI CCGG 3 cut(s) 477, 666, 785
MspR9I CCNGG 5 cut(s) 171, 527, 543, 572, 786
MvaI CCWGG 4 cut(s) 171, 527, 543, 572
MvnI CGCG 1 cut(s) 767
MwoI GCNNNNNNNGC 3 cut(s) 730, 741, 773
NaeI GCCGGC 2 cut(s) 478, 667
NciI CCSGG 1 cut(s) 786
NdeII GATC 1 cut(s) 262
NgoMIV GCCGGC 2 cut(s) 476, 665
NlaIII CATG 6 cut(s) 42, 211, 259, 484, 701, 927
NlaIV GGNNCC 1 cut(s) 540
NmeAIII GCCGAG 2 cut(s) 258, 760
NspI RCATGY 1 cut(s) 484
PaeR7I CTCGAG 1 cut(s) 75
PdiI GCCGGC 2 cut(s) 478, 667
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 3 cut(s) 369, 811, 900
PflMI CCANNNNNTGG 1 cut(s) 703
PkrI GCNGC 4 cut(s) 364, 403, 723, 726
PleI GAGTC 2 cut(s) 16, 211
PpsI GAGTC 2 cut(s) 16, 211
Ppu21I YACGTR 2 cut(s) 448, 824
Psp124BI GAGCTC 1 cut(s) 793
Psp6I CCWGG 4 cut(s) 169, 525, 541, 570
PspGI CCWGG 4 cut(s) 169, 525, 541, 570
PspN4I GGNNCC 1 cut(s) 540
PspPI GGNCC 2 cut(s) 50, 691
PspXI VCTCGAGB 1 cut(s) 75
RsaI GTAC 5 cut(s) 442, 450, 576, 659, 822
RsaNI GTAC 5 cut(s) 441, 449, 575, 658, 821
RseI CAYNNNNRTG 4 cut(s) 37, 69, 378, 800
SacI GAGCTC 1 cut(s) 793
SatI GCNGC 4 cut(s) 363, 402, 722, 725
Sau3AI GATC 1 cut(s) 262
Sau96I GGNCC 2 cut(s) 50, 691
SchI GAGTC 2 cut(s) 16, 212
ScrFI CCNGG 5 cut(s) 171, 527, 543, 572, 786
SduI GDGCHC 3 cut(s) 352, 429, 793
SexAI ACCWGGT 1 cut(s) 570
SfaNI GCATC 1 cut(s) 61
Sfr274I CTCGAG 1 cut(s) 75
SinI GGWCC 1 cut(s) 691
SlaI CTCGAG 1 cut(s) 75
SmiMI CAYNNNNRTG 4 cut(s) 37, 69, 378, 800
SmlI CTYRAG 2 cut(s) 75, 422
SmoI CTYRAG 2 cut(s) 75, 422
Sse9I AATT 1 cut(s) 632
SsiI CCGC 1 cut(s) 724
SstI GAGCTC 1 cut(s) 793
StyD4I CCNGG 5 cut(s) 169, 525, 541, 570, 784
TaaI ACNGT 2 cut(s) 657, 757
TaiI ACGT 4 cut(s) 450, 705, 826, 894
TaqI TCGA 4 cut(s) 76, 201, 636, 869
TasI AATT 1 cut(s) 632
TatI WGTACW 1 cut(s) 657
TauI GCSGC 1 cut(s) 727
TfiI GAWTC 3 cut(s) 369, 811, 900
TscAI CASTG 2 cut(s) 401, 762
TseI GCWGC 3 cut(s) 362, 401, 721
TspDTI ATGAA 4 cut(s) 530, 633, 654, 841
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 2 cut(s) 401, 762
Van91I CCANNNNNTGG 1 cut(s) 703
VpaK11BI GGWCC 1 cut(s) 691
XapI RAATTY 1 cut(s) 632
XceI RCATGY 1 cut(s) 484
XhoI CTCGAG 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 604
ZraI GACGTC 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.