Rmu_sc0025557.1_g000003

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0025557.1
OG0022181
Physical Location & Seq
Reverse (-)
5290 .. 6030
741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0025557.1_g000003.1.cds

Sequence Viewer

Length: 741 bp
atgaaagagttgtttggagttgtgccaaggggggagcattttgcctgcatgattgatcttcttggcagagcaggaaagataagcgaggcaatggagcttgtcaacacaatgccatttcaagcaaacgcctcagtttgggggtcccttcttggtgccgcaagaatccataaaaatgttgatcttggtgaacgtgctgctgatatgctcttagttctggagcctgagaaatccggtacccatgtacttcttgcaaacatttatgcagcagctggcatgtgggacaaggtagcaaaaatgagaagacttatgaaaaataaccaggtaaagaaggaacctggaatgagttggattgaggtccaagacaaggtacacacctttattgtaggagatagaagtcattcaagaagcacggagatatatgcaaaacttgatgagctgcttgaccttgtgcataaagctggctatgttccaatggtggagattgacctccatgatgtggaaccaagtgagaaggagcgacttctgcgataccacagtgagaagcttgcagtggctttcgcattgattgtcactccgcctggagctcccattagagtgaagaagaatttaagagtatgtgtagattgccatactgcattcaagttcatctgtaaaattgtctcgagggagataatcgtgagggacatcaatagattccatcatttcaaagatggctcatgctcttgtggggattactggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

246

Amino Acids

27.77

Weight (kDa)

8.47

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022181)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g08730
malus_domestica MD05G1311200.v1.1
pyrus_communis pycom14g06810
rosa_multiflora Rmu_sc0025557.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 2 cut(s) 152, 233
AccB7I CCANNNNNTGG 1 cut(s) 496
AciI CCGC 2 cut(s) 156, 575
AcsI RAATTY 1 cut(s) 604
AfaI GTAC 3 cut(s) 235, 243, 369
AfiI CCNNNNNNNGG 3 cut(s) 135, 364, 496
AgsI TTSAA 4 cut(s) 119, 402, 640, 706
AjnI CCWGG 3 cut(s) 318, 334, 577
AluBI AGCT 6 cut(s) 97, 269, 436, 458, 544, 584
AluI AGCT 6 cut(s) 97, 269, 436, 458, 544, 584
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 1 cut(s) 664
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 661
ApeKI GCWGC 4 cut(s) 194, 263, 266, 436
ApoI RAATTY 1 cut(s) 604
Asp700I GAANNNNTTC 1 cut(s) 397
Asp718I GGTACC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 141, 355
AsuHPI GGTGA 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 661
AvaII GGWCC 2 cut(s) 141, 355
BaeI ACNNNNGTAYC 4 cut(s) 225, 258, 520, 553
BanI GGYRCC 2 cut(s) 152, 233
BanII GRGCYC 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 4 cut(s) 181, 275, 278, 423
BccI CCATC 2 cut(s) 704, 705
BciT130I CCWGG 3 cut(s) 320, 336, 579
BcoDI GTCTC 1 cut(s) 664
BisI GCNGC 5 cut(s) 156, 195, 264, 267, 437
BlsI GCNGC 5 cut(s) 157, 196, 265, 268, 438
Bme1390I CCNGG 3 cut(s) 320, 336, 579
Bme18I GGWCC 2 cut(s) 141, 355
BmeT110I CYCGRG 1 cut(s) 661
BmgT120I GGNCC 2 cut(s) 141, 355
BmiI GGNNCC 7 cut(s) 142, 143, 154, 219, 235, 333, 501
BmrFI CCNGG 3 cut(s) 320, 336, 579
BpiI GAAGAC 1 cut(s) 307
BpmI CTGGAG 2 cut(s) 236, 600
BsaJI CCNNGG 1 cut(s) 26
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 364, 496
Bse1I ACTGG 1 cut(s) 740
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 3 cut(s) 320, 336, 579
BseDI CCNNGG 1 cut(s) 26
BseLI CCNNNNNNNGG 3 cut(s) 135, 364, 496
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 2 cut(s) 144, 213
BseNI ACTGG 1 cut(s) 740
BseXI GCAGC 4 cut(s) 181, 275, 278, 423
BshNI GGYRCC 2 cut(s) 152, 233
BsiHKAI GWGCWC 1 cut(s) 586
BsiHKCI CYCGRG 1 cut(s) 661
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 3 cut(s) 127, 293, 695
BslI CCNNNNNNNGG 3 cut(s) 135, 364, 496
BsmAI GTCTC 1 cut(s) 664
BsmFI GGGAC 3 cut(s) 127, 293, 695
BsmI GAATGC 1 cut(s) 635
BsoBI CYCGRG 1 cut(s) 661
Bsp1286I GDGCHC 1 cut(s) 586
Bsp143I GATC 2 cut(s) 55, 178
BspACI CCGC 2 cut(s) 156, 575
BspCNI CTCAG 2 cut(s) 143, 214
BspLI GGNNCC 7 cut(s) 142, 143, 154, 219, 235, 333, 501
BspT107I GGYRCC 2 cut(s) 152, 233
BsrDI GCAATG 1 cut(s) 96
BsrI ACTGG 1 cut(s) 740
BssECI CCNNGG 1 cut(s) 26
BssMI GATC 2 cut(s) 55, 178
BssT1I CCWWGG 1 cut(s) 26
Bst2UI CCWGG 3 cut(s) 320, 336, 579
Bst4CI ACNGT 1 cut(s) 536
BstC8I GCNNGC 4 cut(s) 46, 271, 460, 546
BstDEI CTNAG 3 cut(s) 130, 208, 222
BstKTI GATC 2 cut(s) 58, 181
BstMAI GTCTC 1 cut(s) 664
BstMBI GATC 2 cut(s) 55, 178
BstMWI GCNNNNNNNGC 1 cut(s) 523
BstNI CCWGG 3 cut(s) 320, 336, 579
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 3 cut(s) 318, 334, 577
BstV1I GCAGC 4 cut(s) 181, 275, 278, 423
BstV2I GAAGAC 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 2 cut(s) 541, 555
Cac8I GCNNGC 4 cut(s) 46, 271, 460, 546
CaiI CAGNNNCTG 1 cut(s) 269
Cfr13I GGNCC 2 cut(s) 141, 355
CsiI ACCWGGT 1 cut(s) 318
Csp6I GTAC 3 cut(s) 234, 242, 368
CviAII CATG 5 cut(s) 49, 239, 274, 491, 717
CviQI GTAC 3 cut(s) 234, 242, 368
DdeI CTNAG 3 cut(s) 130, 208, 222
DpnI GATC 2 cut(s) 57, 180
DpnII GATC 2 cut(s) 55, 178
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 584
Eco130I CCWWGG 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 586
Eco47I GGWCC 2 cut(s) 141, 355
Eco53kI GAGCTC 1 cut(s) 584
Eco88I CYCGRG 1 cut(s) 661
EcoICRI GAGCTC 1 cut(s) 584
EcoO109I RGGNCCY 1 cut(s) 141
EcoRII CCWGG 3 cut(s) 318, 334, 577
EcoT14I CCWWGG 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 586
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 5 cut(s) 52, 242, 277, 494, 720
FaqI GGGAC 3 cut(s) 127, 293, 695
FatI CATG 5 cut(s) 48, 238, 273, 490, 716
Fnu4HI GCNGC 5 cut(s) 156, 195, 264, 267, 437
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 5 cut(s) 156, 195, 264, 267, 437
GluI GCNGC 5 cut(s) 156, 195, 264, 267, 437
GsuI CTGGAG 2 cut(s) 236, 600
HapII CCGG 1 cut(s) 231
Hin1II CATG 5 cut(s) 52, 242, 277, 494, 720
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 2 cut(s) 162, 693
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 3 cut(s) 103, 188, 370
Hpy188III TCNNGA 4 cut(s) 215, 402, 661, 676
Hpy8I GTNNAC 3 cut(s) 103, 188, 370
HpyAV CCTTC 3 cut(s) 155, 322, 505
HpyCH4III ACNGT 1 cut(s) 536
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 7 cut(s) 48, 251, 263, 422, 451, 548, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 523
HpyF3I CTNAG 3 cut(s) 130, 208, 222
HpySE526I ACGT 1 cut(s) 190
Hsp92II CATG 5 cut(s) 52, 242, 277, 494, 720
KflI GGGWCCC 1 cut(s) 141
KpnI GGTACC 1 cut(s) 237
Kzo9I GATC 2 cut(s) 55, 178
LmnI GCTCC 6 cut(s) 34, 94, 217, 514, 581, 589
Lsp1109I GCAGC 4 cut(s) 181, 275, 278, 423
MabI ACCWGGT 1 cut(s) 318
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 1 cut(s) 568
MalI GATC 2 cut(s) 57, 180
MboI GATC 2 cut(s) 55, 178
MboII GAAGA 4 cut(s) 50, 312, 610, 613
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 2 cut(s) 604, 654
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 6 cut(s) 79, 139, 346, 497, 657, 672
MroXI GAANNNNTTC 1 cut(s) 397
MseI TTAA 1 cut(s) 608
MslI CAYNNNNRTG 2 cut(s) 171, 593
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 3 cut(s) 320, 336, 579
Mva1269I GAATGC 1 cut(s) 635
MvaI CCWGG 3 cut(s) 320, 336, 579
MwoI GCNNNNNNNGC 1 cut(s) 523
NdeII GATC 2 cut(s) 55, 178
NlaIII CATG 5 cut(s) 52, 242, 277, 494, 720
NlaIV GGNNCC 7 cut(s) 142, 143, 154, 219, 235, 333, 501
NmuCI GTSAC 1 cut(s) 568
NspI RCATGY 1 cut(s) 277
PaeR7I CTCGAG 1 cut(s) 661
PctI GAATGC 1 cut(s) 635
PdmI GAANNNNTTC 1 cut(s) 397
PfeI GAWTC 2 cut(s) 162, 693
PflMI CCANNNNNTGG 1 cut(s) 496
PkrI GCNGC 5 cut(s) 157, 196, 265, 268, 438
PpuMI RGGWCCY 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 586
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 3 cut(s) 318, 334, 577
PspGI CCWGG 3 cut(s) 318, 334, 577
PspN4I GGNNCC 7 cut(s) 142, 143, 154, 219, 235, 333, 501
PspPI GGNCC 2 cut(s) 141, 355
PspPPI RGGWCCY 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 3 cut(s) 235, 243, 369
RsaNI GTAC 3 cut(s) 234, 242, 368
RseI CAYNNNNRTG 2 cut(s) 171, 593
SacI GAGCTC 1 cut(s) 586
SaqAI TTAA 1 cut(s) 608
SatI GCNGC 5 cut(s) 156, 195, 264, 267, 437
Sau3AI GATC 2 cut(s) 55, 178
Sau96I GGNCC 2 cut(s) 141, 355
ScrFI CCNGG 3 cut(s) 320, 336, 579
SduI GDGCHC 1 cut(s) 586
SexAI ACCWGGT 1 cut(s) 318
Sfr274I CTCGAG 1 cut(s) 661
SinI GGWCC 2 cut(s) 141, 355
SlaI CTCGAG 1 cut(s) 661
SmiMI CAYNNNNRTG 2 cut(s) 171, 593
SmlI CTYRAG 1 cut(s) 661
SmoI CTYRAG 1 cut(s) 661
Sse9I AATT 2 cut(s) 604, 654
SsiI CCGC 2 cut(s) 156, 575
SstI GAGCTC 1 cut(s) 586
StyD4I CCNGG 3 cut(s) 318, 334, 577
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 536
TaiI ACGT 1 cut(s) 193
TaqI TCGA 1 cut(s) 662
TasI AATT 2 cut(s) 604, 654
TatI WGTACW 1 cut(s) 241
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 2 cut(s) 162, 693
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 2 cut(s) 541, 555
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 4 cut(s) 194, 263, 266, 436
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 3 cut(s) 17, 323, 634
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 2 cut(s) 541, 555
Van91I CCANNNNNTGG 1 cut(s) 496
VpaK11BI GGWCC 2 cut(s) 141, 355
XapI RAATTY 1 cut(s) 604
XceI RCATGY 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 661
XmnI GAANNNNTTC 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.