MD05G1354100.v1.1
HSP70 Family

Heat shock protein 70 (HSP70)-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011323
Physical Location & Seq
Reverse (-)
47073992 .. 47076295
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1354100.v1.1.491

Sequence Viewer

Length: 2304 bp
ATGGGGAAATCAGGGGGTAGGAAGAAGAAGGGAGCTTCGAACCAGGTCCCGAAGGATGCGAATTCGACTCCGATTTCGAACGGTAGTGTTGATTTTGACTCTTCAATCTTTTTGAAAAGAGCCCACGAGCTCAAAGAAGAAGGGAACAAGAGATTTCAGGGAAAGGACTATGTGGGTGCTCTGGACCAGTATGATAATGCTCTTAAGCTCACTCCCAAGAACCACCCTGATCGCGCGGTTTTCCATAGCAATAGGGCGGCTTGTTTGATGCAAATGAAGCCGATAGATTATGAGACTGTCATTGCTGAGTGCACCATGGCGCTCCAGGTGCAGCCCCGGTATGTCAGGGCTCTGCTTCGGAGGGCAAGGGCGCTGGATGCGATAGGGAAGTATGAAATGGCGATGCAGGATGTTCAGGTGTTATTGGGTGCTGACCCGAATCACAGGGATGCTTTGGAGATTGCCCAGCGGTTGAGGACAGCGCTTGGGCTGCGTCAGGAGGTTCAGCAGGACCTCCAGAGCCGCCCATCACCTGCTGCTCTTGGGGCTTCTGCTGTTCGCGGTGCTCCAGTTGGTGGGTTAGGGCCTTCTTTACCTGCCCGACCGGTTCTAAAGAAGGCAGCTGCTTCAGCTGGGGGCTCTGGTATATCCCCAATTAGTAGGACTGAGAAGCCACAAACAAGTTTACCGGCTGAGAATGGACCTGAGAACAAAACCCAAATGCCAAAGCTCACATTGAAGCCGTCCAATGGTCCTTCAAAATCTGCTAATCCAGGGAAGGGTATCCTAATGGAGCCATCGTCTTCATCAGTTTCATTGCCCGTGAATAGGCAGCTTTCAGAGGCTGCAACTCGTTGGAGACCATTGAAGCTTGTTTATGATCATGACATAAGGCTTGCCCAAATGCCTGTGAATAGCAATTTCAGAGTGTTGAGGGAAGTTGTGACGAAGCGCTTTCCATCATCAAAGTCAGTTTTGGTCAAGTATAAGGATAATGATGGTGACATGGTGACTATAACCTCTACAGCTGAACTTAGGTTGGCTGAGTCTTGTGCTGACAAGGTCATTTCAGAAGAGCCTGAAATAGATAAAGCTGACTCAATTGGGATGTTGAGATTGCATATCGTGGACGTGACTCCTGAACAGGAACCGCCTTTATTGGAAGAAGAGGAGGAAAAACCTGTAGAAAGCGAGGGTGTCAAGGAAGATGAAAGTAACTCATCACTTGGTGAATCTGTTTTGGAGCCTGCTGATTATGAAACTGTTAAGTCAGAGAAAGAAGCTCCAAAGGAGAAACCAGGAGCTTCAGAAGATCCCGAGTGCAAGGAATTGGAGATGGATGACTGGTTGTTTGAGTTTTCTCAGCTTTTCCGCAGCCATGTTGGTATTGACCCAGACGCTCATATTGATTTGCATGAACTTGGAATGGAGCTTTGTTCGGAGGCTCTTGAAGAGGCAGTTACTAGTGAAGAAGCTCAGAGTCTTTTTGACAAGGCTGCCTCAAAGTTCCAGGAGGTGGCGGCATTAGCGTTCTTTAATTGGGGAAATGTTTATATGTGTGCGGCAAGGAAGCGGATTCCCTTAGATGAGTCTGCTGGAAAGGAGGTCGTGGCAACGCAGCTTCAAACGGCTTATGACTGGGTCAGGGACAAATATTCCTTGGCCAGAGAGAAGTATGAGGAGGCACTTGTGATCAAGCCAGACTTCTACGAGGGGTTGCTGGCGCTGGGGCAGCAACAATTTGAAATGGCCAAACTTCATTGGTCATTTGCACTTGCTAGGAAAATAGATCTCTCGACATGTGATTCTACAGAAATGCTGACACTTTTTGACAGTGCAGAGGAGAAGATGAAAGTTGCAACTGAGATGTGGGAGAAGCTGGAGGAGCAGAGAGCAAAGGAACTGAAGGATCCAAGTGCAGCCAAGAGGGAAGAGTTGTTGAAAAAAAGGAAAAAACAGGGTACCGGGAATGAAGGTGAGGCATCTGGTCAGGGTGAGTTATCGGCAGATGAAGCTGCAGAACAGGCAGCTGTTATGAGATCACAGATCCATCTCTTCTGGGGTAACATGCTTTTTGAGCGATCCCAGGTCGAATGCAAATTAGGGCTGGATGGTTGGAAGAAAAACCTGGACGCTGCTGTTGAGCGCTTTAAGCTTGCTGGAGCTTCTGAGGGTGACATTTCAATGGTTCTGAAGAATCACTTCTCCAATGTCGATAGAGTGGAGGGGGATGAGAAAAAGGTTCTAGGCAGTGACGCGCCTGTTGAAGCCGACAAAGACGATGATATTCTTTCAGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

768

Amino Acids

84.7

Weight (kDa)

5.21

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 288 - 377 1.3e-14 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62390
fragaria_vesca FvH4_3g01270 FvH4_3g01270 FvH4_3g01270
malus_domestica MD05G1354100.v1.1 MD10G1328200.v1.1
prunus_persica Prupe.4G013700_v2.0.a1 Prupe.4G013700_v2.0.a1
pyrus_communis pycom05g32150 pycom10g27810
rosa_chinensis RchiOBHm_Chr5g0001821
rosa_laevigata RLG00000030974
rosa_multiflora Rmu_ssc0000237.1_g000011
rosa_roxburghii Rroxscaffold_1G00074530
rosa_rugosa Rorug04G0393400
rosa_samantha Rh5AG014600 Rh5BG018000 Rh5CG015700 Rh5DG016000
rosa_wichuraiana Rw5G001400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 541
Acc36I ACCTGC 2 cut(s) 541, 604
Acc65I GGTACC 1 cut(s) 1961
AccB1I GGYRCC 1 cut(s) 1961
AccB7I CCANNNNNTGG 2 cut(s) 575, 1516
AccII CGCG 4 cut(s) 234, 236, 561, 2258
AclWI GGATC 5 cut(s) 1307, 1904, 1917, 2041, 2076
AcoI YGGCCR 2 cut(s) 1662, 1749
AcsI RAATTY 1 cut(s) 61
AcuI CTGAAG 4 cut(s) 612, 1290, 1925, 2213
AdeI CACNNNGTG 1 cut(s) 1229
AfaI GTAC 1 cut(s) 1963
AfeI AGCGCT 3 cut(s) 483, 953, 2147
AfiI CCNNNNNNNGG 5 cut(s) 575, 749, 779, 828, 1516
AflII CTTAAG 1 cut(s) 203
AflIII ACRYGT 1 cut(s) 1799
AgeI ACCGGT 1 cut(s) 604
AhlI ACTAGT 1 cut(s) 1463
AjiI CACGTC 1 cut(s) 1132
AjnI CCWGG 7 cut(s) 42, 324, 772, 1297, 1509, 2085, 2127
Alw21I GWGCWC 4 cut(s) 132, 181, 314, 568
Alw26I GTCTC 2 cut(s) 287, 853
Alw44I GTGCAC 1 cut(s) 310
AlwI GGATC 5 cut(s) 1307, 1904, 1917, 2041, 2076
AlwNI CAGNNNCTG 1 cut(s) 845
Ama87I CYCGRG 1 cut(s) 1316
Aor51HI AGCGCT 3 cut(s) 483, 953, 2147
AoxI GGCC 3 cut(s) 584, 1662, 1749
ApaLI GTGCAC 1 cut(s) 310
ApoI RAATTY 1 cut(s) 61
ArsI GACNNNNNNTTYG 4 cut(s) 58, 90, 1334, 1366
AsiGI ACCGGT 1 cut(s) 604
Asp700I GAANNNNTTC 1 cut(s) 2201
Asp718I GGTACC 1 cut(s) 1961
AspLEI GCGC 8 cut(s) 236, 322, 373, 484, 954, 1726, 2148, 2260
AspS9I GGNCC 6 cut(s) 46, 184, 511, 584, 701, 752
AsuC2I CCSGG 2 cut(s) 337, 1966
AsuHPI GGTGA 7 cut(s) 522, 1013, 1021, 1241, 1988, 2006, 2186
AsuII TTCGAA 2 cut(s) 38, 77
AvaI CYCGRG 1 cut(s) 1316
AvaII GGWCC 5 cut(s) 46, 184, 511, 701, 752
BaeGI GKGCMC 1 cut(s) 314
BalI TGGCCA 2 cut(s) 1664, 1751
BamHI GGATCC 1 cut(s) 1909
BanI GGYRCC 1 cut(s) 1961
BanII GRGCYC 4 cut(s) 124, 132, 352, 641
BauI CACGAG 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 795
Bbv12I GWGCWC 4 cut(s) 132, 181, 314, 568
BccI CCATC 7 cut(s) 535, 805, 967, 992, 1330, 2058, 2105
BceAI ACGGC 2 cut(s) 727, 1644
BciT130I CCWGG 7 cut(s) 44, 326, 774, 1299, 1511, 2087, 2129
BciVI GTATCC 1 cut(s) 794
BclI TGATCA 2 cut(s) 880, 1692
BcnI CCSGG 2 cut(s) 337, 1966
BcoDI GTCTC 2 cut(s) 287, 853
BcuI ACTAGT 1 cut(s) 1463
BfaI CTAG 3 cut(s) 1464, 1779, 2246
BfmI CTRYAG 4 cut(s) 1023, 1182, 1809, 2016
BfoI RGCGCY 6 cut(s) 323, 374, 485, 955, 1727, 2149
BfrI CTTAAG 1 cut(s) 203
BfuAI ACCTGC 2 cut(s) 541, 604
BfuI GTATCC 1 cut(s) 794
BglII AGATCT 1 cut(s) 1789
Bme1390I CCNGG 9 cut(s) 44, 326, 337, 774, 1299, 1511, 1966, 2087, 2129
Bme18I GGWCC 5 cut(s) 46, 184, 511, 701, 752
BmeT110I CYCGRG 1 cut(s) 1316
BmgBI CACGTC 1 cut(s) 1132
BmgT120I GGNCC 6 cut(s) 46, 184, 511, 584, 701, 752
BmiI GGNNCC 6 cut(s) 48, 795, 1149, 1245, 1911, 1963
BmrFI CCNGG 9 cut(s) 44, 326, 337, 774, 1299, 1511, 1966, 2087, 2129
BmrI ACTGGG 1 cut(s) 1648
BmsI GCATC 6 cut(s) 46, 258, 367, 393, 439, 1991
BmuI ACTGGG 1 cut(s) 1648
BpiI GAAGAC 1 cut(s) 795
BpmI CTGGAG 5 cut(s) 308, 500, 552, 1901, 2181
Bpu14I TTCGAA 2 cut(s) 38, 77
BpuMI CCSGG 2 cut(s) 337, 1966
BsaI GGTCTC 1 cut(s) 853
BsaJI CCNNGG 5 cut(s) 315, 335, 773, 1659, 2085
BsaWI WCCGGW 1 cut(s) 604
BsaXI ACNNNNNCTCC 2 cut(s) 785, 815
Bsc4I CCNNNNNNNGG 5 cut(s) 575, 749, 779, 828, 1516
Bse118I RCCGGY 2 cut(s) 604, 688
Bse1I ACTGG 4 cut(s) 187, 569, 1349, 1643
Bse3DI GCAATG 2 cut(s) 300, 815
BseBI CCWGG 7 cut(s) 44, 326, 774, 1299, 1511, 2087, 2129
BseDI CCNNGG 5 cut(s) 315, 335, 773, 1659, 2085
BseGI GGATG 8 cut(s) 61, 382, 415, 454, 1113, 1345, 2116, 2236
BseLI CCNNNNNNNGG 5 cut(s) 575, 749, 779, 828, 1516
BseMI GCAATG 2 cut(s) 300, 815
BseMII CTCAG 9 cut(s) 297, 657, 684, 696, 1035, 1376, 1490, 1854, 2160
BseNI ACTGG 4 cut(s) 187, 569, 1349, 1643
BseRI GAGGAG 4 cut(s) 1184, 1694, 1856, 1898
BseSI GKGCMC 1 cut(s) 314
BseYI CCCAGC 3 cut(s) 465, 632, 1726
BsgI GTGCAG 3 cut(s) 350, 1857, 1938
Bsh1236I CGCG 4 cut(s) 234, 236, 561, 2258
Bsh1285I CGRYCG 1 cut(s) 605
BshFI GGCC 3 cut(s) 586, 1664, 1751
BshNI GGYRCC 1 cut(s) 1961
BshTI ACCGGT 1 cut(s) 604
BsiEI CGRYCG 1 cut(s) 605
BsiHKAI GWGCWC 4 cut(s) 132, 181, 314, 568
BsiHKCI CYCGRG 1 cut(s) 1316
BsiSI CCGG 4 cut(s) 337, 605, 689, 1965
BslFI GGGAC 2 cut(s) 32, 1661
BslI CCNNNNNNNGG 5 cut(s) 575, 749, 779, 828, 1516
BsmAI GTCTC 2 cut(s) 287, 853
BsmFI GGGAC 2 cut(s) 32, 1661
BsmI GAATGC 1 cut(s) 2099
BsnI GGCC 3 cut(s) 586, 1664, 1751
Bso31I GGTCTC 1 cut(s) 853
BsoBI CYCGRG 1 cut(s) 1316
Bsp119I TTCGAA 2 cut(s) 38, 77
Bsp1286I GDGCHC 7 cut(s) 124, 132, 181, 314, 352, 568, 641
Bsp143I GATC 9 cut(s) 229, 880, 1312, 1692, 1789, 1909, 2039, 2046, 2081
Bsp19I CCATGG 1 cut(s) 315
BspANI GGCC 3 cut(s) 586, 1664, 1751
BspCNI CTCAG 9 cut(s) 298, 658, 685, 697, 1036, 1375, 1489, 1855, 2161
BspFNI CGCG 4 cut(s) 234, 236, 561, 2258
BspHI TCATGA 1 cut(s) 883
BspLI GGNNCC 6 cut(s) 48, 795, 1149, 1245, 1911, 1963
BspMAI CTGCAG 1 cut(s) 2020
BspMI ACCTGC 2 cut(s) 541, 604
BspPI GGATC 5 cut(s) 1307, 1904, 1917, 2041, 2076
BspQI GCTCTTC 1 cut(s) 1068
BspT104I TTCGAA 2 cut(s) 38, 77
BspT107I GGYRCC 1 cut(s) 1961
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 853
BsrDI GCAATG 2 cut(s) 300, 815
BsrFI RCCGGY 2 cut(s) 604, 688
BsrI ACTGG 4 cut(s) 187, 569, 1349, 1643
BssAI RCCGGY 2 cut(s) 604, 688
BssECI CCNNGG 5 cut(s) 315, 335, 773, 1659, 2085
BssMI GATC 9 cut(s) 229, 880, 1312, 1692, 1789, 1909, 2039, 2046, 2081
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 2 cut(s) 315, 1659
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 7 cut(s) 44, 326, 774, 1299, 1511, 2087, 2129
Bst4CI ACNGT 4 cut(s) 83, 298, 1264, 1835
Bst6I CTCTTC 6 cut(s) 106, 1068, 1161, 1446, 1926, 2060
BstAFI CTTAAG 1 cut(s) 203
BstBI TTCGAA 2 cut(s) 38, 77
BstC8I GCNNGC 4 cut(s) 897, 1248, 1722, 2157
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 8 cut(s) 61, 382, 415, 454, 1113, 1345, 2116, 2236
BstFNI CGCG 4 cut(s) 234, 236, 561, 2258
BstH2I RGCGCY 6 cut(s) 323, 374, 485, 955, 1727, 2149
BstHHI GCGC 8 cut(s) 236, 322, 373, 484, 954, 1726, 2148, 2260
BstKTI GATC 9 cut(s) 232, 883, 1315, 1695, 1792, 1912, 2042, 2049, 2084
BstMAI GTCTC 2 cut(s) 287, 853
BstMBI GATC 9 cut(s) 229, 880, 1312, 1692, 1789, 1909, 2039, 2046, 2081
BstMCI CGRYCG 1 cut(s) 605
BstNI CCWGG 7 cut(s) 44, 326, 774, 1299, 1511, 2087, 2129
BstNSI RCATGY 2 cut(s) 1803, 2071
BstSCI CCNGG 9 cut(s) 42, 324, 335, 772, 1297, 1509, 1964, 2085, 2127
BstSFI CTRYAG 4 cut(s) 1023, 1182, 1809, 2016
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 4 cut(s) 234, 236, 561, 2258
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 4 cut(s) 1312, 1789, 1909, 2046
BstYI RGATCY 4 cut(s) 1312, 1789, 1909, 2046
BsuI GTATCC 1 cut(s) 794
BsuRI GGCC 3 cut(s) 586, 1664, 1751
BtgI CCRYGG 1 cut(s) 315
BtgZI GCGATG 1 cut(s) 416
BtrI CACGTC 1 cut(s) 1132
BtsCI GGATG 8 cut(s) 61, 382, 415, 454, 1113, 1345, 2116, 2236
BtsI GCAGTG 1 cut(s) 2257
BtsIMutI CAGTG 2 cut(s) 1840, 2257
BveI ACCTGC 2 cut(s) 541, 604
Cac8I GCNNGC 4 cut(s) 897, 1248, 1722, 2157
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 1 cut(s) 883
CfoI GCGC 8 cut(s) 236, 322, 373, 484, 954, 1726, 2148, 2260
Cfr10I RCCGGY 2 cut(s) 604, 688
Cfr13I GGNCC 6 cut(s) 46, 184, 511, 584, 701, 752
CseI GACGC 4 cut(s) 482, 1406, 2141, 2264
CsiI ACCWGGT 1 cut(s) 42
Csp6I GTAC 1 cut(s) 1962
CspAI ACCGGT 1 cut(s) 604
CviAII CATG 7 cut(s) 316, 884, 1006, 1379, 1415, 1800, 2068
CviQI GTAC 1 cut(s) 1962
DpnI GATC 9 cut(s) 231, 882, 1314, 1694, 1791, 1911, 2041, 2048, 2083
DpnII GATC 9 cut(s) 229, 880, 1312, 1692, 1789, 1909, 2039, 2046, 2081
DraIII CACNNNGTG 1 cut(s) 1229
EaeI YGGCCR 2 cut(s) 1662, 1749
Eam1104I CTCTTC 6 cut(s) 106, 1068, 1161, 1446, 1926, 2060
EarI CTCTTC 6 cut(s) 106, 1068, 1161, 1446, 1926, 2060
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 315, 1659
Eco24I GRGCYC 4 cut(s) 124, 132, 352, 641
Eco31I GGTCTC 1 cut(s) 853
Eco47I GGWCC 5 cut(s) 46, 184, 511, 701, 752
Eco47III AGCGCT 3 cut(s) 483, 953, 2147
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 4 cut(s) 612, 1290, 1925, 2213
Eco88I CYCGRG 1 cut(s) 1316
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 3 cut(s) 46, 511, 584
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 7 cut(s) 42, 324, 772, 1297, 1509, 2085, 2127
EcoT14I CCWWGG 2 cut(s) 315, 1659
EcoT38I GRGCYC 4 cut(s) 124, 132, 352, 641
ErhI CCWWGG 2 cut(s) 315, 1659
FaeI CATG 7 cut(s) 319, 887, 1009, 1382, 1418, 1803, 2071
FalI AAGNNNNNCTT 4 cut(s) 1688, 1720, 2186, 2218
FaqI GGGAC 2 cut(s) 32, 1661
FatI CATG 7 cut(s) 315, 883, 1005, 1378, 1414, 1799, 2067
FbaI TGATCA 2 cut(s) 880, 1692
FokI GGATG 8 cut(s) 68, 389, 422, 461, 1120, 1352, 2123, 2243
FriOI GRGCYC 4 cut(s) 124, 132, 352, 641
FspBI CTAG 3 cut(s) 1464, 1779, 2246
GlaI GCGC 8 cut(s) 235, 321, 372, 483, 953, 1725, 2147, 2259
GsaI CCCAGC 3 cut(s) 469, 636, 1730
GsuI CTGGAG 5 cut(s) 308, 500, 552, 1901, 2181
HaeII RGCGCY 6 cut(s) 323, 374, 485, 955, 1727, 2149
HaeIII GGCC 3 cut(s) 586, 1664, 1751
HapII CCGG 4 cut(s) 337, 605, 689, 1965
HgaI GACGC 4 cut(s) 482, 1406, 2141, 2264
HhaI GCGC 8 cut(s) 236, 322, 373, 484, 954, 1726, 2148, 2260
Hin1II CATG 7 cut(s) 319, 887, 1009, 1382, 1418, 1803, 2071
Hin6I GCGC 8 cut(s) 234, 320, 371, 482, 952, 1724, 2146, 2258
HinP1I GCGC 8 cut(s) 234, 320, 371, 482, 952, 1724, 2146, 2258
HindIII AAGCTT 2 cut(s) 869, 2153
HpaII CCGG 4 cut(s) 337, 605, 689, 1965
HphI GGTGA 7 cut(s) 522, 1013, 1021, 1241, 1988, 2006, 2186
Hpy166II GTNNAC 3 cut(s) 312, 686, 1129
Hpy188III TCNNGA 9 cut(s) 49, 182, 497, 517, 884, 1139, 1316, 1448, 1795
Hpy8I GTNNAC 3 cut(s) 312, 686, 1129
HpyAV CCTTC 9 cut(s) 22, 46, 134, 597, 610, 765, 772, 1900, 1967
HpyCH4III ACNGT 4 cut(s) 83, 298, 1264, 1835
HpyCH4IV ACGT 1 cut(s) 1131
HpySE526I ACGT 1 cut(s) 1131
Hsp92II CATG 7 cut(s) 319, 887, 1009, 1382, 1418, 1803, 2071
HspAI GCGC 8 cut(s) 234, 320, 371, 482, 952, 1724, 2146, 2258
KpnI GGTACC 1 cut(s) 1965
Ksp22I TGATCA 2 cut(s) 880, 1692
Kzo9I GATC 9 cut(s) 229, 880, 1312, 1692, 1789, 1909, 2039, 2046, 2081
LguI GCTCTTC 1 cut(s) 1068
LweI GCATC 6 cut(s) 46, 258, 367, 393, 439, 1991
MabI ACCWGGT 1 cut(s) 42
MaeI CTAG 3 cut(s) 1464, 1779, 2246
MaeII ACGT 1 cut(s) 1131
MaeIII GTNAC 9 cut(s) 943, 1001, 1009, 1132, 1214, 1459, 2063, 2174, 2252
MalI GATC 9 cut(s) 231, 882, 1314, 1694, 1791, 1911, 2041, 2048, 2083
MboI GATC 9 cut(s) 229, 880, 1312, 1692, 1789, 1909, 2039, 2046, 2081
MfeI CAATTG 1 cut(s) 1101
MflI RGATCY 4 cut(s) 1312, 1789, 1909, 2046
MhlI GDGCHC 7 cut(s) 124, 132, 181, 314, 352, 568, 641
MlsI TGGCCA 2 cut(s) 1664, 1751
MluCI AATT 8 cut(s) 61, 654, 919, 1101, 1328, 1537, 1739, 2099
MluNI TGGCCA 2 cut(s) 1664, 1751
MlyI GAGTC 7 cut(s) 61, 92, 1055, 1091, 1129, 1489, 1598
MmeI TCCRAC 2 cut(s) 836, 2096
Mox20I TGGCCA 2 cut(s) 1664, 1751
MroXI GAANNNNTTC 1 cut(s) 2201
MscI TGGCCA 2 cut(s) 1664, 1751
MseI TTAA 4 cut(s) 204, 1266, 1536, 2151
MslI CAYNNNNRTG 2 cut(s) 447, 2183
Msp20I TGGCCA 2 cut(s) 1664, 1751
MspA1I CMGCKG 5 cut(s) 469, 623, 632, 1028, 2030
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 4 cut(s) 337, 605, 689, 1965
MspR9I CCNGG 9 cut(s) 44, 326, 337, 774, 1299, 1511, 1966, 2087, 2129
MunI CAATTG 1 cut(s) 1101
Mva1269I GAATGC 1 cut(s) 2099
MvaI CCWGG 7 cut(s) 44, 326, 774, 1299, 1511, 2087, 2129
MvnI CGCG 4 cut(s) 234, 236, 561, 2258
NciI CCSGG 2 cut(s) 337, 1966
NcoI CCATGG 1 cut(s) 315
NdeII GATC 9 cut(s) 229, 880, 1312, 1692, 1789, 1909, 2039, 2046, 2081
NlaIII CATG 7 cut(s) 319, 887, 1009, 1382, 1418, 1803, 2071
NlaIV GGNNCC 6 cut(s) 48, 795, 1149, 1245, 1911, 1963
NmuCI GTSAC 6 cut(s) 943, 1001, 1009, 1132, 2174, 2252
NspI RCATGY 2 cut(s) 1803, 2071
NspV TTCGAA 2 cut(s) 38, 77
PagI TCATGA 1 cut(s) 883
PaqCI CACCTGC 1 cut(s) 541
PciI ACATGT 1 cut(s) 1799
PciSI GCTCTTC 1 cut(s) 1068
PctI GAATGC 1 cut(s) 2099
PdmI GAANNNNTTC 1 cut(s) 2201
PfeI GAWTC 5 cut(s) 439, 1232, 1576, 1805, 2197
PflFI GACNNNGTC 2 cut(s) 1061, 1640
PflMI CCANNNNNTGG 2 cut(s) 575, 1516
PfoI TCCNGGA 1 cut(s) 1509
PinAI ACCGGT 1 cut(s) 604
PleI GAGTC 7 cut(s) 61, 92, 1054, 1091, 1129, 1488, 1597
PpsI GAGTC 7 cut(s) 61, 92, 1054, 1091, 1129, 1488, 1597
PpuMI RGGWCCY 2 cut(s) 46, 511
PscI ACATGT 1 cut(s) 1799
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 2 cut(s) 46, 511
Psp6I CCWGG 7 cut(s) 42, 324, 772, 1297, 1509, 2085, 2127
PspFI CCCAGC 3 cut(s) 465, 632, 1726
PspGI CCWGG 7 cut(s) 42, 324, 772, 1297, 1509, 2085, 2127
PspN4I GGNNCC 6 cut(s) 48, 795, 1149, 1245, 1911, 1963
PspPI GGNCC 6 cut(s) 46, 184, 511, 584, 701, 752
PspPPI RGGWCCY 2 cut(s) 46, 511
PstI CTGCAG 1 cut(s) 2020
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 4 cut(s) 1312, 1789, 1909, 2046
PsyI GACNNNGTC 2 cut(s) 1061, 1640
PvuII CAGCTG 4 cut(s) 623, 632, 1028, 2030
RsaI GTAC 1 cut(s) 1963
RsaNI GTAC 1 cut(s) 1962
RseI CAYNNNNRTG 2 cut(s) 447, 2183
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 1068
SaqAI TTAA 4 cut(s) 204, 1266, 1536, 2151
Sau3AI GATC 9 cut(s) 229, 880, 1312, 1692, 1789, 1909, 2039, 2046, 2081
Sau96I GGNCC 6 cut(s) 46, 184, 511, 584, 701, 752
SchI GAGTC 7 cut(s) 61, 92, 1055, 1091, 1129, 1489, 1598
ScrFI CCNGG 9 cut(s) 44, 326, 337, 774, 1299, 1511, 1966, 2087, 2129
SduI GDGCHC 7 cut(s) 124, 132, 181, 314, 352, 568, 641
SexAI ACCWGGT 1 cut(s) 42
SfaNI GCATC 6 cut(s) 46, 258, 367, 393, 439, 1991
SfcI CTRYAG 4 cut(s) 1023, 1182, 1809, 2016
SfuI TTCGAA 2 cut(s) 38, 77
SinI GGWCC 5 cut(s) 46, 184, 511, 701, 752
SmiMI CAYNNNNRTG 2 cut(s) 447, 2183
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SpeI ACTAGT 1 cut(s) 1463
Sse9I AATT 8 cut(s) 61, 654, 919, 1101, 1328, 1537, 1739, 2099
SspI AATATT 1 cut(s) 1655
SspMI CTAG 3 cut(s) 1464, 1779, 2246
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 9 cut(s) 42, 324, 335, 772, 1297, 1509, 1964, 2085, 2127
StyI CCWWGG 2 cut(s) 315, 1659
TaaI ACNGT 4 cut(s) 83, 298, 1264, 1835
TaiI ACGT 1 cut(s) 1134
TaqI TCGA 6 cut(s) 38, 65, 77, 1796, 2091, 2214
TasI AATT 8 cut(s) 61, 654, 919, 1101, 1328, 1537, 1739, 2099
TauI GCSGC 4 cut(s) 260, 525, 1523, 1565
TfiI GAWTC 5 cut(s) 439, 1232, 1576, 1805, 2197
Tru1I TTAA 4 cut(s) 204, 1266, 1536, 2151
Tru9I TTAA 4 cut(s) 204, 1266, 1536, 2151
TscAI CASTG 2 cut(s) 1840, 2257
TseFI GTSAC 6 cut(s) 943, 1001, 1009, 1132, 2174, 2252
Tsp45I GTSAC 6 cut(s) 943, 1001, 1009, 1132, 2174, 2252
TspRI CASTG 2 cut(s) 1840, 2257
Tth111I GACNNNGTC 2 cut(s) 1061, 1640
Van91I CCANNNNNTGG 2 cut(s) 575, 1516
Vha464I CTTAAG 1 cut(s) 203
VneI GTGCAC 1 cut(s) 310
VpaK11BI GGWCC 5 cut(s) 46, 184, 511, 701, 752
XapI RAATTY 1 cut(s) 61
XceI RCATGY 2 cut(s) 1803, 2071
XmnI GAANNNNTTC 1 cut(s) 2201
XspI CTAG 3 cut(s) 1464, 1779, 2246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.