pycom10g27810
HSP70 Family

Heat shock protein 70 (HSP70)-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011323
Physical Location & Seq
Reverse (-)
28688786 .. 28691080
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g27810.1

Sequence Viewer

Length: 2295 bp
ATGGGGAAATCAGGTGGTAGGAAGAAGAAGGGAGCTTCGAACCAGGTCCCGACGGATGCGAATTCGACTCCGATTGTAAACGGTAGTGTTGATTTGGACAATTCGATCTTTTTGAAAAGAGCCCACGAGCTCAAAGAAGAAGGGAACAAGAGATTTCAGGGGAAGGACTATGTGGGTGCTCTGGACCAGTATGATAGTGCTCTTAAGCTCACTCCCAAGACCCACCCGGACCGGGCGGTTTTCCATAGCAATAAGGCGGCTTGTTTGATGCAAATGAAGCCAATAGATTATGAGACTGTCATTGCTGAGTGCACCCTGGCGCTCCAGGTGCAGCCCCGGTATGTCAGGGCTCTGCTTCGGAGGGCGAGGGCATTGGAGGCGATCGGGAAGTATGAAATGGCTATGCAGGATGTTCAGGTGTTATTGGTTGCTGACCCAACTCACCGGGATGCTTTGGAGATTGCCCAGCGGTTGAGGACAGCGCTTGGCCTGCGTCAGGAGGCTCAGCAGGACCTCCAGAGCCGCCCGTCACCTGCTGCCCTTGGGGCTTCTGCTGTTCGCGGTGCTCCAGTTGGTGGGTTGGGGCCTTGTTTACCAGCCCGGCCTGTTCCAAAGAAGGCAGCTGCTTCAGCTGGTGGTTCTGGTATATCCCCAAGTAATAGGACCGAGAAGCCTCAAACAAGTTTACTGGCTGAGAACGGACCTTTGAACAAAACCCAAATGCCAAAACTCACATTGAAGCCGTCAAATGGTCCTTCAAAATCTGCCAATCCAAGTAAGGGTAACCTGAAGGAGCCGTCATCTTCAGTTTCATTGCCAGAGGCTGCAACTCGTTGGAGACCATTGAAGCTTGTTTATGATCATGACATAAGGCTTGCCCAAATGCCTGTGAATTGTAATTTCAGAGTATTGAGGGAAATTGTGACGAAGCGCTTTCCATCATCAAAGTCAGTTTTGGTCAAGTATAAGGATAGTGATGGTGATCTGGTGACTATAACCTCTACTGCTGAACTTAGGTTGGCTGAGTCTTGTGTTGACAAGGTCATTTTAGAAGAGCCTGAAATAGATAAAGCTGATTCTATTGGGATGTTGAGATTGCATATCGTGGAAGTGACTCCTGAACAGGAACCGCCTTTATTGGAAGAAGAGGAGGAAAAACCTGTCGAAACCGAGGGGATCAAGGAAGATGAAAGTAACTCTAATTCTTCACTTGGTGAATCTGTTTTGGAGCCTGCTGATTATGAAACTGATAAGACAGAGAAAGAAGTTCCAAAGGAGAAACCAGGAGCTGCAGAAGATCCTGAGTGCAAGGAATTGGAGATGGATGATTGGTTGTTTGAGTTTTCTCAGCTTTTCCGCAGCCATGTTGGTATTGACCCAGACGCTCATATTGATTTGCATGAGCTTGGAATGGAGCTTTGTTCGGAGGCTCTTGAAGAGGCAGTTACTAGTGAAGAAGCTCAGAGTCTTTTTGACAAGGCTGCCTCAAAGTTCCAGGAGGTGGCGGCATTAGCTTTCTTTAATTGGGGAAATGTTTATATGTGTGCGGCAAGGAAGCGGATTCCCTTAGATGAGTCTGCCGGAAAGGATGTAGTGGCTATGCAGCTTCAAATGGCCTATGACTGGGTCAGGGAAAAATATTCCTTGGCCAGAGAGAAGTATGAAGAGGCACTTGTGATCAAGCCAGATTTTTACGAGGGGTTGCTGGCGCTGGGGCAGCAACAATTTGAAATGGCCAAACTTCATTGGTCATTTGCACTTGCTAGGAAAATAGATCTCGCGACCTGTGATTCTGCAGAAATGCTGACACTTTTTGACAGTGCGGAGGCGAAGATGAGAGTTGCAACTGAGATGTGGGAGAAGCTGGAGGAGCAGAGGGCAAAGGAACTGAAGGATCCAAGTGCAAGCAAGAGGGATGAGTTGATGAAAAAAAGGAAAAAACAAGGTACCGGGAATGAAGGTGATGCATCTGGTCAGGGTGAGATTTCAGCAGATGAAGCTGCAGAACAGGCAGCTGTTATGAGATCACAGATCCATCTCTTCTGGGGTAACATGCTTTTTGAGCGATCCCAGGTCGAATGCAAATTGGGGATGGATGGTTGGAAGAAAAACCTGGACGATGCTGTGGAGCGCTTTAAGCTTGCTGGAGCTTCTGAGGGTGACATCGCATTGGTTCTGAAGAATCATTTCTCCAACGTCGAAGGAGTGGAGGGGGATGAGAAAAAGGTTCAGATTCTAGGCAGTGACGTTCCTGTTGAAGCCAATAAAGATGATGAGATTCTTTCAGGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

765

Amino Acids

84.39

Weight (kDa)

5.18

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 281 - 370 1.8e-14 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62390
fragaria_vesca FvH4_3g01270 FvH4_3g01270 FvH4_3g01270
malus_domestica MD05G1354100.v1.1 MD10G1328200.v1.1
prunus_persica Prupe.4G013700_v2.0.a1 Prupe.4G013700_v2.0.a1
pyrus_communis pycom05g32150 pycom10g27810
rosa_chinensis RchiOBHm_Chr5g0001821
rosa_laevigata RLG00000030974
rosa_multiflora Rmu_ssc0000237.1_g000011
rosa_roxburghii Rroxscaffold_1G00074530
rosa_rugosa Rorug04G0393400
rosa_samantha Rh5AG014600 Rh5BG018000 Rh5CG015700 Rh5DG016000
rosa_wichuraiana Rw5G001400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 541
Acc36I ACCTGC 1 cut(s) 541
Acc65I GGTACC 1 cut(s) 1946
AccB1I GGYRCC 1 cut(s) 1946
AccB7I CCANNNNNTGG 2 cut(s) 575, 1501
AccII CGCG 2 cut(s) 561, 1781
AclWI GGATC 6 cut(s) 1184, 1292, 1889, 1902, 2026, 2061
AcoI YGGCCR 2 cut(s) 1647, 1734
AcsI RAATTY 1 cut(s) 61
AcuI CTGAAG 5 cut(s) 612, 789, 809, 1910, 2198
AdeI CACNNNGTG 1 cut(s) 1214
AfaI GTAC 1 cut(s) 1948
AfeI AGCGCT 3 cut(s) 483, 932, 2132
AfiI CCNNNNNNNGG 7 cut(s) 232, 496, 575, 749, 779, 1501, 1623
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 9 cut(s) 115, 709, 739, 759, 847, 1436, 1610, 1730, 2258
AhlI ACTAGT 1 cut(s) 1448
AjnI CCWGG 7 cut(s) 42, 315, 324, 1282, 1494, 2070, 2112
Alw21I GWGCWC 5 cut(s) 132, 181, 202, 314, 568
Alw26I GTCTC 2 cut(s) 287, 832
Alw44I GTGCAC 1 cut(s) 310
AlwI GGATC 6 cut(s) 1184, 1292, 1889, 1902, 2026, 2061
AlwNI CAGNNNCTG 2 cut(s) 824, 1289
Aor51HI AGCGCT 3 cut(s) 483, 932, 2132
AoxI GGCC 6 cut(s) 487, 584, 602, 1614, 1647, 1734
ApaLI GTGCAC 1 cut(s) 310
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 2186
Asp718I GGTACC 1 cut(s) 1946
AspLEI GCGC 5 cut(s) 322, 484, 933, 1711, 2133
AspS9I GGNCC 8 cut(s) 46, 184, 229, 511, 584, 663, 701, 752
AsuC2I CCSGG 6 cut(s) 227, 233, 337, 446, 601, 1951
AsuHPI GGTGA 8 cut(s) 434, 522, 992, 1000, 1226, 1973, 1991, 2171
AsuII TTCGAA 1 cut(s) 38
AvaII GGWCC 7 cut(s) 46, 184, 229, 511, 663, 701, 752
BaeGI GKGCMC 1 cut(s) 314
BalI TGGCCA 2 cut(s) 1649, 1736
BamHI GGATCC 1 cut(s) 1894
BanI GGYRCC 1 cut(s) 1946
BanII GRGCYC 3 cut(s) 124, 132, 352
BauI CACGAG 1 cut(s) 125
Bbv12I GWGCWC 5 cut(s) 132, 181, 202, 314, 568
BccI CCATC 6 cut(s) 946, 971, 1315, 2043, 2086, 2090
BceAI ACGGC 2 cut(s) 727, 781
BciT130I CCWGG 7 cut(s) 44, 317, 326, 1284, 1496, 2072, 2114
BclI TGATCA 2 cut(s) 859, 1677
BcnI CCSGG 6 cut(s) 227, 233, 337, 446, 601, 1951
BcoDI GTCTC 2 cut(s) 287, 832
BcuI ACTAGT 1 cut(s) 1448
BfaI CTAG 3 cut(s) 1449, 1764, 2237
BfmI CTRYAG 3 cut(s) 1290, 1794, 2001
BfoI RGCGCY 5 cut(s) 323, 485, 934, 1712, 2134
BfrI CTTAAG 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 541
BglI GCCNNNNNGGC 1 cut(s) 545
BglII AGATCT 1 cut(s) 1774
BlpI GCTNAGC 1 cut(s) 504
Bme18I GGWCC 7 cut(s) 46, 184, 229, 511, 663, 701, 752
BmgT120I GGNCC 8 cut(s) 46, 184, 229, 511, 584, 663, 701, 752
BmiI GGNNCC 7 cut(s) 48, 585, 795, 1128, 1230, 1896, 1948
BmrI ACTGGG 1 cut(s) 1633
BmsI GCATC 6 cut(s) 46, 258, 439, 1954, 1976, 2110
BmuI ACTGGG 1 cut(s) 1633
BpmI CTGGAG 5 cut(s) 308, 500, 552, 1886, 2166
Bpu1102I GCTNAGC 1 cut(s) 504
Bpu14I TTCGAA 1 cut(s) 38
BpuMI CCSGG 6 cut(s) 227, 233, 337, 446, 601, 1951
BsaBI GATNNNNATC 1 cut(s) 981
BsaI GGTCTC 1 cut(s) 832
BsaJI CCNNGG 6 cut(s) 315, 335, 541, 1170, 1644, 2070
Bsc4I CCNNNNNNNGG 7 cut(s) 232, 496, 575, 749, 779, 1501, 1623
Bse1I ACTGG 4 cut(s) 187, 569, 693, 1628
Bse3DI GCAATG 2 cut(s) 300, 812
Bse8I GATNNNNATC 1 cut(s) 981
BseBI CCWGG 7 cut(s) 44, 317, 326, 1284, 1496, 2072, 2114
BseDI CCNNGG 6 cut(s) 315, 335, 541, 1170, 1644, 2070
BseJI GATNNNNATC 1 cut(s) 981
BseLI CCNNNNNNNGG 7 cut(s) 232, 496, 575, 749, 779, 1501, 1623
BseMI GCAATG 2 cut(s) 300, 812
BseMII CTCAG 9 cut(s) 297, 518, 684, 1014, 1293, 1361, 1475, 1839, 2145
BseNI ACTGG 4 cut(s) 187, 569, 693, 1628
BseRI GAGGAG 2 cut(s) 1163, 1883
BseSI GKGCMC 1 cut(s) 314
BseYI CCCAGC 2 cut(s) 465, 1711
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 2 cut(s) 561, 1781
Bsh1285I CGRYCG 1 cut(s) 384
BshFI GGCC 6 cut(s) 489, 586, 604, 1616, 1649, 1736
BshNI GGYRCC 1 cut(s) 1946
BsiEI CGRYCG 1 cut(s) 384
BsiHKAI GWGCWC 5 cut(s) 132, 181, 202, 314, 568
BsiSI CCGG 7 cut(s) 227, 232, 337, 445, 601, 1581, 1950
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 7 cut(s) 232, 496, 575, 749, 779, 1501, 1623
BsmAI GTCTC 2 cut(s) 287, 832
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 2084
BsnI GGCC 6 cut(s) 489, 586, 604, 1616, 1649, 1736
Bso31I GGTCTC 1 cut(s) 832
Bsp119I TTCGAA 1 cut(s) 38
Bsp1286I GDGCHC 7 cut(s) 124, 132, 181, 202, 314, 352, 568
Bsp1720I GCTNAGC 1 cut(s) 504
Bsp68I TCGCGA 1 cut(s) 1781
BspANI GGCC 6 cut(s) 489, 586, 604, 1616, 1649, 1736
BspCNI CTCAG 9 cut(s) 298, 517, 685, 1015, 1294, 1360, 1474, 1840, 2146
BspFNI CGCG 2 cut(s) 561, 1781
BspHI TCATGA 1 cut(s) 862
BspLI GGNNCC 7 cut(s) 48, 585, 795, 1128, 1230, 1896, 1948
BspMAI CTGCAG 3 cut(s) 1294, 1798, 2005
BspMI ACCTGC 1 cut(s) 541
BspPI GGATC 6 cut(s) 1184, 1292, 1889, 1902, 2026, 2061
BspQI GCTCTTC 1 cut(s) 1047
BspT104I TTCGAA 1 cut(s) 38
BspT107I GGYRCC 1 cut(s) 1946
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 832
BsrDI GCAATG 2 cut(s) 300, 812
BsrI ACTGG 4 cut(s) 187, 569, 693, 1628
BssECI CCNNGG 6 cut(s) 315, 335, 541, 1170, 1644, 2070
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 2 cut(s) 541, 1644
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 7 cut(s) 44, 317, 326, 1284, 1496, 2072, 2114
Bst4CI ACNGT 3 cut(s) 83, 298, 1820
Bst6I CTCTTC 5 cut(s) 1047, 1140, 1431, 1659, 2045
BstAFI CTTAAG 1 cut(s) 203
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 6 cut(s) 491, 876, 1233, 1707, 1906, 2142
BstEII GGTNACC 1 cut(s) 782
BstENI CCTNNNNNAGG 1 cut(s) 494
BstFNI CGCG 2 cut(s) 561, 1781
BstH2I RGCGCY 5 cut(s) 323, 485, 934, 1712, 2134
BstHHI GCGC 5 cut(s) 322, 484, 933, 1711, 2133
BstMAI GTCTC 2 cut(s) 287, 832
BstMCI CGRYCG 1 cut(s) 384
BstNI CCWGG 7 cut(s) 44, 317, 326, 1284, 1496, 2072, 2114
BstNSI RCATGY 1 cut(s) 2056
BstPI GGTNACC 1 cut(s) 782
BstSFI CTRYAG 3 cut(s) 1290, 1794, 2001
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 2 cut(s) 561, 1781
BstX2I RGATCY 4 cut(s) 1297, 1774, 1894, 2031
BstYI RGATCY 4 cut(s) 1297, 1774, 1894, 2031
BsuRI GGCC 6 cut(s) 489, 586, 604, 1616, 1649, 1736
BtgZI GCGATG 1 cut(s) 2149
BtsI GCAGTG 1 cut(s) 2248
BtsIMutI CAGTG 2 cut(s) 1825, 2248
BtuMI TCGCGA 1 cut(s) 1781
BveI ACCTGC 1 cut(s) 541
Cac8I GCNNGC 6 cut(s) 491, 876, 1233, 1707, 1906, 2142
CaiI CAGNNNCTG 2 cut(s) 824, 1289
CciI TCATGA 1 cut(s) 862
CfoI GCGC 5 cut(s) 322, 484, 933, 1711, 2133
Cfr13I GGNCC 8 cut(s) 46, 184, 229, 511, 584, 663, 701, 752
CpoI CGGWCCG 1 cut(s) 229
CseI GACGC 2 cut(s) 482, 1391
CsiI ACCWGGT 1 cut(s) 42
Csp6I GTAC 1 cut(s) 1947
CspI CGGWCCG 1 cut(s) 229
CviAII CATG 4 cut(s) 863, 1364, 1400, 2053
CviQI GTAC 1 cut(s) 1947
DraIII CACNNNGTG 1 cut(s) 1214
EaeI YGGCCR 2 cut(s) 1647, 1734
Eam1104I CTCTTC 5 cut(s) 1047, 1140, 1431, 1659, 2045
EarI CTCTTC 5 cut(s) 1047, 1140, 1431, 1659, 2045
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 541, 1644
Eco24I GRGCYC 3 cut(s) 124, 132, 352
Eco31I GGTCTC 1 cut(s) 832
Eco47I GGWCC 7 cut(s) 46, 184, 229, 511, 663, 701, 752
Eco47III AGCGCT 3 cut(s) 483, 932, 2132
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 5 cut(s) 612, 789, 809, 1910, 2198
Eco91I GGTNACC 1 cut(s) 782
EcoICRI GAGCTC 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 494
EcoO109I RGGNCCY 3 cut(s) 46, 511, 584
EcoO65I GGTNACC 1 cut(s) 782
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 7 cut(s) 42, 315, 324, 1282, 1494, 2070, 2112
EcoT14I CCWWGG 2 cut(s) 541, 1644
EcoT22I ATGCAT 1 cut(s) 1969
EcoT38I GRGCYC 3 cut(s) 124, 132, 352
ErhI CCWWGG 2 cut(s) 541, 1644
FaeI CATG 4 cut(s) 866, 1367, 1403, 2056
FalI AAGNNNNNCTT 2 cut(s) 1656, 1688
FaqI GGGAC 1 cut(s) 32
FatI CATG 4 cut(s) 862, 1363, 1399, 2052
FbaI TGATCA 2 cut(s) 859, 1677
FriOI GRGCYC 3 cut(s) 124, 132, 352
FspBI CTAG 3 cut(s) 1449, 1764, 2237
GlaI GCGC 5 cut(s) 321, 483, 932, 1710, 2132
GsaI CCCAGC 2 cut(s) 469, 1715
GsuI CTGGAG 5 cut(s) 308, 500, 552, 1886, 2166
HaeII RGCGCY 5 cut(s) 323, 485, 934, 1712, 2134
HaeIII GGCC 6 cut(s) 489, 586, 604, 1616, 1649, 1736
HapII CCGG 7 cut(s) 227, 232, 337, 445, 601, 1581, 1950
HgaI GACGC 2 cut(s) 482, 1391
HhaI GCGC 5 cut(s) 322, 484, 933, 1711, 2133
Hin1II CATG 4 cut(s) 866, 1367, 1403, 2056
Hin6I GCGC 5 cut(s) 320, 482, 931, 1709, 2131
HinP1I GCGC 5 cut(s) 320, 482, 931, 1709, 2131
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HindIII AAGCTT 2 cut(s) 848, 2138
HpaII CCGG 7 cut(s) 227, 232, 337, 445, 601, 1581, 1950
HphI GGTGA 8 cut(s) 434, 522, 992, 1000, 1226, 1973, 1991, 2171
Hpy166II GTNNAC 5 cut(s) 79, 312, 593, 686, 1036
Hpy188I TCNGA 8 cut(s) 72, 360, 905, 1426, 1464, 2155, 2178, 2232
Hpy8I GTNNAC 5 cut(s) 79, 312, 593, 686, 1036
Hpy99I CGWCG 2 cut(s) 55, 2201
HpyAV CCTTC 9 cut(s) 22, 134, 157, 610, 765, 784, 1885, 1952, 2195
HpyCH4III ACNGT 3 cut(s) 83, 298, 1820
HpyCH4IV ACGT 2 cut(s) 2196, 2247
HpySE526I ACGT 2 cut(s) 2196, 2247
Hsp92II CATG 4 cut(s) 866, 1367, 1403, 2056
HspAI GCGC 5 cut(s) 320, 482, 931, 1709, 2131
KpnI GGTACC 1 cut(s) 1950
Ksp22I TGATCA 2 cut(s) 859, 1677
LguI GCTCTTC 1 cut(s) 1047
LweI GCATC 6 cut(s) 46, 258, 439, 1954, 1976, 2110
MabI ACCWGGT 1 cut(s) 42
MaeI CTAG 3 cut(s) 1449, 1764, 2237
MaeII ACGT 2 cut(s) 2196, 2247
MflI RGATCY 4 cut(s) 1297, 1774, 1894, 2031
MhlI GDGCHC 7 cut(s) 124, 132, 181, 202, 314, 352, 568
MlsI TGGCCA 2 cut(s) 1649, 1736
MluNI TGGCCA 2 cut(s) 1649, 1736
MlyI GAGTC 5 cut(s) 61, 1034, 1108, 1474, 1583
MmeI TCCRAC 3 cut(s) 815, 2081, 2217
Mox20I TGGCCA 2 cut(s) 1649, 1736
Mph1103I ATGCAT 1 cut(s) 1969
MroXI GAANNNNTTC 1 cut(s) 2186
MscI TGGCCA 2 cut(s) 1649, 1736
MseI TTAA 3 cut(s) 204, 1521, 2136
MslI CAYNNNNRTG 1 cut(s) 447
Msp20I TGGCCA 2 cut(s) 1649, 1736
MspA1I CMGCKG 4 cut(s) 469, 623, 632, 2015
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 7 cut(s) 227, 232, 337, 445, 601, 1581, 1950
Mva1269I GAATGC 1 cut(s) 2084
MvaI CCWGG 7 cut(s) 44, 317, 326, 1284, 1496, 2072, 2114
MvnI CGCG 2 cut(s) 561, 1781
NciI CCSGG 6 cut(s) 227, 233, 337, 446, 601, 1951
NlaIII CATG 4 cut(s) 866, 1367, 1403, 2056
NlaIV GGNNCC 7 cut(s) 48, 585, 795, 1128, 1230, 1896, 1948
NmuCI GTSAC 6 cut(s) 528, 922, 988, 1111, 2159, 2243
NruI TCGCGA 1 cut(s) 1781
NsiI ATGCAT 1 cut(s) 1969
NspI RCATGY 1 cut(s) 2056
NspV TTCGAA 1 cut(s) 38
PagI TCATGA 1 cut(s) 862
PaqCI CACCTGC 1 cut(s) 541
PciSI GCTCTTC 1 cut(s) 1047
PctI GAATGC 1 cut(s) 2084
PdmI GAANNNNTTC 1 cut(s) 2186
PfeI GAWTC 7 cut(s) 1076, 1217, 1561, 1790, 2182, 2233, 2278
PflFI GACNNNGTC 2 cut(s) 1040, 1625
PflMI CCANNNNNTGG 2 cut(s) 575, 1501
PfoI TCCNGGA 1 cut(s) 1494
Ple19I CGATCG 1 cut(s) 384
PleI GAGTC 5 cut(s) 61, 1033, 1108, 1473, 1582
PpsI GAGTC 5 cut(s) 61, 1033, 1108, 1473, 1582
PpuMI RGGWCCY 2 cut(s) 46, 511
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 2 cut(s) 46, 511
Psp6I CCWGG 7 cut(s) 42, 315, 324, 1282, 1494, 2070, 2112
PspEI GGTNACC 1 cut(s) 782
PspFI CCCAGC 2 cut(s) 465, 1711
PspGI CCWGG 7 cut(s) 42, 315, 324, 1282, 1494, 2070, 2112
PspN4I GGNNCC 7 cut(s) 48, 585, 795, 1128, 1230, 1896, 1948
PspPI GGNCC 8 cut(s) 46, 184, 229, 511, 584, 663, 701, 752
PspPPI RGGWCCY 2 cut(s) 46, 511
PstI CTGCAG 3 cut(s) 1294, 1798, 2005
PstNI CAGNNNCTG 2 cut(s) 824, 1289
PsuI RGATCY 4 cut(s) 1297, 1774, 1894, 2031
PsyI GACNNNGTC 2 cut(s) 1040, 1625
PvuI CGATCG 1 cut(s) 384
PvuII CAGCTG 3 cut(s) 623, 632, 2015
RruI TCGCGA 1 cut(s) 1781
RsaI GTAC 1 cut(s) 1948
RsaNI GTAC 1 cut(s) 1947
RseI CAYNNNNRTG 1 cut(s) 447
Rsr2I CGGWCCG 1 cut(s) 229
RsrII CGGWCCG 1 cut(s) 229
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 1047
SaqAI TTAA 3 cut(s) 204, 1521, 2136
Sau96I GGNCC 8 cut(s) 46, 184, 229, 511, 584, 663, 701, 752
SchI GAGTC 5 cut(s) 61, 1034, 1108, 1474, 1583
SduI GDGCHC 7 cut(s) 124, 132, 181, 202, 314, 352, 568
SexAI ACCWGGT 1 cut(s) 42
SfaNI GCATC 6 cut(s) 46, 258, 439, 1954, 1976, 2110
SfcI CTRYAG 3 cut(s) 1290, 1794, 2001
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 7 cut(s) 46, 184, 229, 511, 663, 701, 752
SmiMI CAYNNNNRTG 1 cut(s) 447
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
SpeI ACTAGT 1 cut(s) 1448
SspI AATATT 1 cut(s) 1640
SspMI CTAG 3 cut(s) 1449, 1764, 2237
SstI GAGCTC 1 cut(s) 132
StyI CCWWGG 2 cut(s) 541, 1644
TaaI ACNGT 3 cut(s) 83, 298, 1820
TaiI ACGT 2 cut(s) 2199, 2250
TaqI TCGA 6 cut(s) 38, 65, 104, 1164, 2076, 2199
TaqII GACCGA 1 cut(s) 680
TauI GCSGC 4 cut(s) 260, 525, 1508, 1550
TfiI GAWTC 7 cut(s) 1076, 1217, 1561, 1790, 2182, 2233, 2278
Tru1I TTAA 3 cut(s) 204, 1521, 2136
Tru9I TTAA 3 cut(s) 204, 1521, 2136
TscAI CASTG 2 cut(s) 1825, 2248
TseFI GTSAC 6 cut(s) 528, 922, 988, 1111, 2159, 2243
Tsp45I GTSAC 6 cut(s) 528, 922, 988, 1111, 2159, 2243
TspGWI ACGGA 2 cut(s) 68, 714
TspRI CASTG 2 cut(s) 1825, 2248
Tth111I GACNNNGTC 2 cut(s) 1040, 1625
Van91I CCANNNNNTGG 2 cut(s) 575, 1501
Vha464I CTTAAG 1 cut(s) 203
VneI GTGCAC 1 cut(s) 310
VpaK11BI GGWCC 7 cut(s) 46, 184, 229, 511, 663, 701, 752
XagI CCTNNNNNAGG 1 cut(s) 494
XapI RAATTY 1 cut(s) 61
XceI RCATGY 1 cut(s) 2056
XmnI GAANNNNTTC 1 cut(s) 2186
XspI CTAG 3 cut(s) 1449, 1764, 2237
Zsp2I ATGCAT 1 cut(s) 1969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.