MD06G1056000.v1.1

Calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0022315
Physical Location & Seq
Forward (+)
8384935 .. 8386271
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1056000.v1.1.491

Sequence Viewer

Length: 249 bp
ATGGCAGATCAATTGACAGATGATCAGCTGAACATGCTCAAGACTAAATTCAATACCTTCGACCCAGATGGCACTGGCATAATCACTAGGGATCAGTTGGCCGATGTGTTGAGATCGTTAGGGCAAGATCCCACCGAGGAGGAGTTGGATCAATTCGTGCAGCAGCTGGGCAGTGAGGATGGGAATATTCACTGGGATAAATTCGTCGAACTCATGGCCCAGACAATATCTGCATTAGCTGGGCAGTGA

Protein Analysis

83

Amino Acids

9.17

Weight (kDa)

4.05

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 12 - 72 1.1e-09 EF-hand domain pair
EF-hand_6 PF13405 13 - 41 2.5e-06 EF-hand domain
EF-hand_9 PF14658 14 - 75 6e-08 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022315)

Species Orthologous Gene IDs
malus_domestica MD06G1056000.v1.1 MD06G1056200.v1.1
prunus_persica Prupe.5G066700_v2.0.a1 Prupe.5G066900_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 99, 122, 156
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 2 cut(s) 47, 200
AgsI TTSAA 1 cut(s) 52
AluBI AGCT 3 cut(s) 28, 166, 239
AluI AGCT 3 cut(s) 28, 166, 239
AlwI GGATC 3 cut(s) 99, 122, 156
AlwNI CAGNNNCTG 1 cut(s) 166
AoxI GGCC 2 cut(s) 99, 216
ApeKI GCWGC 2 cut(s) 160, 163
ApoI RAATTY 2 cut(s) 47, 200
AspS9I GGNCC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 172, 175
BccI CCATC 2 cut(s) 62, 173
BclI TGATCA 1 cut(s) 22
BfaI CTAG 1 cut(s) 87
BisI GCNGC 2 cut(s) 161, 164
BlsI GCNGC 2 cut(s) 162, 165
BmgT120I GGNCC 1 cut(s) 217
BmrI ACTGGG 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 135
Bse1I ACTGG 2 cut(s) 79, 197
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 1 cut(s) 184
BseNI ACTGG 2 cut(s) 79, 197
BseRI GAGGAG 2 cut(s) 152, 155
BseXI GCAGC 2 cut(s) 172, 175
BseYI CCCAGC 2 cut(s) 166, 239
BsgI GTGCAG 1 cut(s) 179
BshFI GGCC 2 cut(s) 101, 218
BsnI GGCC 2 cut(s) 101, 218
Bsp143I GATC 6 cut(s) 7, 22, 91, 113, 127, 148
BspANI GGCC 2 cut(s) 101, 218
BspPI GGATC 3 cut(s) 99, 122, 156
BsrI ACTGG 2 cut(s) 79, 197
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 6 cut(s) 7, 22, 91, 113, 127, 148
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 6 cut(s) 10, 25, 94, 116, 130, 151
BstMBI GATC 6 cut(s) 7, 22, 91, 113, 127, 148
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNSI RCATGY 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 172, 175
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BsuRI GGCC 2 cut(s) 101, 218
BtsCI GGATG 1 cut(s) 184
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 3 cut(s) 72, 178, 190
CaiI CAGNNNCTG 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 217
CviAII CATG 2 cut(s) 34, 214
CviJI RGCY 5 cut(s) 28, 101, 166, 218, 239
CviKI_1 RGCY 5 cut(s) 28, 101, 166, 218, 239
DpnI GATC 6 cut(s) 9, 24, 93, 115, 129, 150
DpnII GATC 6 cut(s) 7, 22, 91, 113, 127, 148
EaeI YGGCCR 1 cut(s) 99
FaeI CATG 2 cut(s) 37, 217
FaiI YATR 3 cut(s) 35, 80, 215
FatI CATG 2 cut(s) 33, 213
FbaI TGATCA 1 cut(s) 22
Fnu4HI GCNGC 2 cut(s) 161, 164
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 161, 164
FspBI CTAG 1 cut(s) 87
GluI GCNGC 2 cut(s) 161, 164
GsaI CCCAGC 2 cut(s) 170, 243
HaeIII GGCC 2 cut(s) 101, 218
Hin1II CATG 2 cut(s) 37, 217
Hpy188III TCNNGA 1 cut(s) 40
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 67
HpyCH4V TGCA 2 cut(s) 160, 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
Hsp92II CATG 2 cut(s) 37, 217
Ksp22I TGATCA 1 cut(s) 22
Kzo9I GATC 6 cut(s) 7, 22, 91, 113, 127, 148
LpnPI CCDG 6 cut(s) 60, 78, 152, 178, 225, 233
Lsp1109I GCAGC 2 cut(s) 172, 175
MaeI CTAG 1 cut(s) 87
MalI GATC 6 cut(s) 9, 24, 93, 115, 129, 150
MboI GATC 6 cut(s) 7, 22, 91, 113, 127, 148
MfeI CAATTG 1 cut(s) 11
MflI RGATCY 1 cut(s) 127
MluCI AATT 4 cut(s) 11, 47, 152, 200
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 3 cut(s) 130, 133, 169
MspA1I CMGCKG 2 cut(s) 28, 166
MunI CAATTG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 34
NdeII GATC 6 cut(s) 7, 22, 91, 113, 127, 148
NlaIII CATG 2 cut(s) 37, 217
NspI RCATGY 1 cut(s) 37
PkrI GCNGC 2 cut(s) 162, 165
PspFI CCCAGC 2 cut(s) 166, 239
PspPI GGNCC 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 127
PvuII CAGCTG 2 cut(s) 28, 166
SatI GCNGC 2 cut(s) 161, 164
Sau3AI GATC 6 cut(s) 7, 22, 91, 113, 127, 148
Sau96I GGNCC 1 cut(s) 217
SetI ASST 4 cut(s) 30, 59, 168, 241
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 4 cut(s) 11, 47, 152, 200
SspI AATATT 1 cut(s) 187
SspMI CTAG 1 cut(s) 87
TaqI TCGA 2 cut(s) 60, 207
TasI AATT 4 cut(s) 11, 47, 152, 200
TscAI CASTG 3 cut(s) 79, 178, 197
TseI GCWGC 2 cut(s) 160, 163
TspRI CASTG 3 cut(s) 79, 178, 197
XapI RAATTY 2 cut(s) 47, 200
XceI RCATGY 1 cut(s) 37
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.