Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0007356

Physical Location & Seq

Genomic Coordinates Reverse (-)

20400919 .. 20405536

Gene Structure 4618 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1083100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGGCAGGGGGCAACAAACTGCATAAGAATGATCAACCTTCGATCAAATACAGGTGGTACCCAGTTGGTCAGGCATCCTCTGGGCTTCCTGGTTTTGTTCATGAAGAATCTGCCTCCGCCATTTCTGATAAAGTTAATAAGCAATGCAGTAGAAAGTTGCTGCCTAAGCCTCGTGAGATATTGAGCACTGCTGAGCTCATCACAGCAGTTGGCCAGATATGGGATTGTGCAAGTCGTCCTCTTGGTTTTTTTCAACCCAAGGCACATTCAAACCAAAATGACGGTGGCTGTCAGAAGGATGTTATCTTGGCTGATATAGGTGGGGAAGGAAATGACACGGCACCTATTTCAGCCAAGAGTGAGTACTTGTCTGTTGATTTCAGGAATTTTGGTCCATTTCCACCCACAATCCAATCGAGTTTAGATTTTCTGCAGGTAAGTGAAAAGATTTCAGTGTTCGAACCTTGCAGTAGAAGTTATTCTCATTCATCATTTTGGAGGTTTTTGCATGATGGTGCGAGTGTGTCTCAAGAATCTTGGAGGGGAAATGGGCTTGCAAGTTTAGAAGTCTCATACCAATTGGGAAGTATATATGGATGGATGGGTGATATGATTTCTTCTGGGTTAAACTGCCCAGTCAAAGTTACTGAGATTGATAACAGAACTGGTCAGTACTGCATTCCAGGAGATACAGTGAGCATTGCAGGCCAATCTATTTCAGGAGATACAACTAGTCATTCAGAGAAATTGGCCGCAAATGCTGAAAATCCTGCTGTAATTGAATGTTACAAATACCATTTATCATCATATGAGAATGGAAACTTGGAGATGGATACAAGCACCTCTCTGTCTTCTGATTACTACCTGAGTTCTGTAGGCAGTGTGGGGGATGATAGTATTTCAAGGACCCCATACTCTAATCCATATACTGACTATCATATAGACTCGATTGTTTCCTGTAATGATGTGTTTGACGAATGTCATCCTAAGACAGATCATCATGAACTACTTGGGAACCAAAAGAAACAATCCGAAAAGTATATTGTCCAAGATGAATGCAAGGCAGAGATCTGTGCATTAGCATGTGAAAAGCGTCGTTATGCTCTTCCTAAGCAAGAGCATGCCTTTGCAGGTGCATTGTCTGGAGTATTTGTTAGCATTTGTCTTCATCCTGTTGATACAATTAAGACTGTTGTTCAATCCTGCAGAGCTGAGCAGAAGTCTATATACGACATTGGAAAATCAATTGTGTCTGATAGAGGTTTAACTGGACTTTATCGTGGTATTGCTACCAACATTGCATCATCAGCTCCAATTTCTGCAGTTTATACTTTCACATATGAATCAGTCAAGGGAGCTTTACTTCCTCTTTTTCCAAAGGAGTATTACTCTTTTGCCCACTGCGTGGCAGGCGGTTGTGCAAGCATTGCTACTTCCTTTATTTTTACTCCTAGTGAGCGTATAAAGCAGCAGATGCAAGTTGGTTCACACTATCACAGCTGCTGGAATGCATTTGTTGGAATCATTCGGAAGGGAGGTTTGCCATCACTGTATGCTGGATGGGGAGCTGTACTATGCAGGAATGTTCCTCACTCAATTGTCAAGTTCTACACATATGAAAGCCTGAAGCAATTTATGCTGTCTCCAAAAGAAGCTGGTGTCCAACCCACCACATTACAAACACTAGTTTGTGGAGGAATAGCGGGATCTACTGCTGCTTTATTTACAACTCCTTTTGATGTTGTGAAGACAAGATTGCAGACTCAGATTCCGGGGTCTATGAGCCAGTATAATGGTGTGATTCATGCCCTTCAAGAAATAGGAAAGAATGAAGGTCTGCAAGGCCTCTACAGGGGATTGACTCCGAGATTGATTATGTATATGTCTCAAGGAGCGATCTTCTTTGCATCGTATGAATTTTTCAAGAGATTGTTCTCTTTGGAGAGTCCCCAAACCGATGTTCAAAGAATTCAATACAAGCAAAAGATGGACAATGATCCAGTGCCGCCATCATCAATCCCATCACCATCTTCACAAGCATCATCTGAACTGCATGCTCAACATTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mito_carr

Protein Analysis

690

Amino Acids

75.45

Weight (kDa)

6.38

Isoelectric Point (pI)

47.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mito_carr	PF00153	373 - 454	2e-17	Mitochondrial carrier protein
Mito_carr	PF00153	463 - 547	6.8e-18	Mitochondrial carrier protein
Mito_carr	PF00153	556 - 646	3e-27	Mitochondrial carrier protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007356)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11440 AT4G11440
fragaria_vesca	FvH4_5g02410 FvH4_5g02410 FvH4_5g02410 FvH4_5g02410 FvH4_5g02410 FvH4_5g02410 FvH4_5g02410
malus_domestica	MD06G1083100.v1.1
prunus_persica	Prupe.5G100200_v2.0.a1 Prupe.5G100200_v2.0.a1
pyrus_communis	pycom06g08000
rosa_chinensis	RchiOBHm_Chr7g0197691
rosa_laevigata	RLG00000003919
rosa_multiflora	Rmu_sc0001164.1_g000021 Rmu_sc0006732.1_g000008 Rmu_sc0029952.1_g000002
rosa_roxburghii	Rroxscaffold_3G00258290
rosa_rugosa	Rorug07G0042200
rosa_samantha	Rh7AG166600 Rh7BG168800 Rh7CG175100 Rh7DG168400
rosa_wichuraiana	Rw7G014510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1121
Acc36I	ACCTGC	2 cut(s)	424, 1121
Acc65I	GGTACC	1 cut(s)	57
AccB1I	GGYRCC	2 cut(s)	57, 340
AccB7I	CCANNNNNTGG	1 cut(s)	1405
AciI	CCGC	5 cut(s)	117, 753, 1413, 1703, 2006
AclWI	GGATC	2 cut(s)	1714, 1991
AcoI	YGGCCR	2 cut(s)	211, 750
AcsI	RAATTY	3 cut(s)	385, 1916, 1968
AcuI	CTGAAG	1 cut(s)	1646
AdeI	CACNNNGTG	1 cut(s)	1405
AfaI	GTAC	4 cut(s)	59, 365, 674, 1572
AfiI	CCNNNNNNNGG	3 cut(s)	220, 1405, 1852
AgsI	TTSAA	9 cut(s)	254, 270, 782, 903, 1199, 1814, 1924, 1964, 1973
AhlI	ACTAGT	2 cut(s)	731, 1684
AjnI	CCWGG	2 cut(s)	88, 682
AjuI	GAANNNNNNNTTGG	4 cut(s)	806, 838, 1944, 1976
AluBI	AGCT	7 cut(s)	196, 1211, 1310, 1358, 1500, 1568, 1655
AluI	AGCT	7 cut(s)	196, 1211, 1310, 1358, 1500, 1568, 1655
Alw21I	GWGCWC	2 cut(s)	188, 198
Alw26I	GTCTC	4 cut(s)	531, 574, 1647, 1890
AlwI	GGATC	2 cut(s)	1714, 1991
AlwNI	CAGNNNCTG	1 cut(s)	1503
AoxI	GGCC	4 cut(s)	211, 706, 750, 1843
ApeKI	GCWGC	4 cut(s)	160, 1468, 1500, 1715
ApoI	RAATTY	3 cut(s)	385, 1916, 1968
Asp700I	GAANNNNTTC	1 cut(s)	478
Asp718I	GGTACC	1 cut(s)	57
AspS9I	GGNCC	2 cut(s)	392, 906
AsuC2I	CCSGG	1 cut(s)	1773
AsuHPI	GGTGA	2 cut(s)	617, 2016
AsuII	TTCGAA	1 cut(s)	459
AvaII	GGWCC	2 cut(s)	392, 906
BalI	TGGCCA	1 cut(s)	213
BanI	GGYRCC	2 cut(s)	57, 340
BanII	GRGCYC	1 cut(s)	198
BarI	GAAGNNNNNNTAC	2 cut(s)	1211, 1243
BauI	CACGAG	1 cut(s)	171
BbsI	GAAGAC	3 cut(s)	843, 1157, 1754
Bbv12I	GWGCWC	2 cut(s)	188, 198
BbvI	GCAGC	4 cut(s)	147, 1480, 1487, 1702
BceAI	ACGGC	1 cut(s)	354
BciT130I	CCWGG	2 cut(s)	90, 684
BciVI	GTATCC	1 cut(s)	826
BclI	TGATCA	1 cut(s)	31
BcnI	CCSGG	1 cut(s)	1773
BcoDI	GTCTC	4 cut(s)	531, 574, 1647, 1890
BcuI	ACTAGT	2 cut(s)	731, 1684
BfaI	CTAG	3 cut(s)	732, 1452, 1685
BfmI	CTRYAG	5 cut(s)	431, 873, 1204, 1320, 1849
BfuAI	ACCTGC	2 cut(s)	424, 1121
BfuI	GTATCC	1 cut(s)	826
BglII	AGATCT	1 cut(s)	1068
BisI	GCNGC	6 cut(s)	161, 753, 1469, 1501, 1716, 2006
BlpI	GCTNAGC	2 cut(s)	192, 1212
BlsI	GCNGC	6 cut(s)	162, 754, 1470, 1502, 1717, 2007
BmcAI	AGTACT	2 cut(s)	365, 674
Bme1390I	CCNGG	3 cut(s)	90, 684, 1773
Bme18I	GGWCC	2 cut(s)	392, 906
BmgT120I	GGNCC	2 cut(s)	392, 906
BmiI	GGNNCC	4 cut(s)	59, 342, 908, 1016
BmrFI	CCNGG	3 cut(s)	90, 684, 1773
BmrI	ACTGGG	2 cut(s)	56, 629
BmsI	GCATC	5 cut(s)	83, 1310, 1464, 1916, 2048
BmuI	ACTGGG	2 cut(s)	56, 629
BoxI	GACNNNNGTC	1 cut(s)	978
BpiI	GAAGAC	3 cut(s)	843, 1157, 1754
BplI	GAGNNNNNCTC	4 cut(s)	511, 543, 1373, 1405
BpmI	CTGGAG	1 cut(s)	1164
Bpu10I	CCTNAGC	2 cut(s)	165, 1110
Bpu1102I	GCTNAGC	2 cut(s)	192, 1212
Bpu14I	TTCGAA	1 cut(s)	459
BpuEI	CTTGAG	2 cut(s)	513, 1872
BpuMI	CCSGG	1 cut(s)	1773
BsaJI	CCNNGG	2 cut(s)	258, 1772
BsaXI	ACNNNNNCTCC	4 cut(s)	678, 708, 1137, 1167
Bsc4I	CCNNNNNNNGG	3 cut(s)	220, 1405, 1852
Bse1I	ACTGG	6 cut(s)	62, 635, 670, 1273, 1786, 2000
Bse3DI	GCAATG	4 cut(s)	149, 699, 1296, 1425
BseBI	CCWGG	2 cut(s)	90, 684
BseDI	CCNNGG	2 cut(s)	258, 1772
BseGI	GGATG	8 cut(s)	74, 304, 602, 606, 895, 982, 1168, 1565
BseLI	CCNNNNNNNGG	3 cut(s)	220, 1405, 1852
BseMI	GCAATG	4 cut(s)	149, 699, 1296, 1425
BseMII	CTCAG	5 cut(s)	183, 639, 857, 1203, 1778
BseNI	ACTGG	6 cut(s)	62, 635, 670, 1273, 1786, 2000
BseXI	GCAGC	4 cut(s)	147, 1480, 1487, 1702
BshFI	GGCC	4 cut(s)	213, 708, 752, 1845
BshNI	GGYRCC	2 cut(s)	57, 340
BsiHKAI	GWGCWC	2 cut(s)	188, 198
BsiSI	CCGG	1 cut(s)	1772
BslFI	GGGAC	1 cut(s)	1932
BslI	CCNNNNNNNGG	3 cut(s)	220, 1405, 1852
BsmAI	GTCTC	4 cut(s)	531, 574, 1647, 1890
BsmFI	GGGAC	1 cut(s)	1932
BsmI	GAATGC	3 cut(s)	678, 1061, 1513
BsnI	GGCC	4 cut(s)	213, 708, 752, 1845
Bsp119I	TTCGAA	1 cut(s)	459
Bsp1286I	GDGCHC	2 cut(s)	188, 198
Bsp143I	GATC	7 cut(s)	31, 42, 994, 1068, 1706, 1896, 1996
Bsp1720I	GCTNAGC	2 cut(s)	192, 1212
BspACI	CCGC	5 cut(s)	117, 753, 1413, 1703, 2006
BspANI	GGCC	4 cut(s)	213, 708, 752, 1845
BspCNI	CTCAG	5 cut(s)	184, 640, 858, 1204, 1777
BspHI	TCATGA	3 cut(s)	100, 1000, 2066
BspLI	GGNNCC	4 cut(s)	59, 342, 908, 1016
BspMAI	CTGCAG	3 cut(s)	435, 1208, 1324
BspMI	ACCTGC	2 cut(s)	424, 1121
BspPI	GGATC	2 cut(s)	1714, 1991
BspQI	GCTCTTC	1 cut(s)	1110
BspT104I	TTCGAA	1 cut(s)	459
BspT107I	GGYRCC	2 cut(s)	57, 340
BsrDI	GCAATG	4 cut(s)	149, 699, 1296, 1425
BsrI	ACTGG	6 cut(s)	62, 635, 670, 1273, 1786, 2000
BssECI	CCNNGG	2 cut(s)	258, 1772
BssMI	GATC	7 cut(s)	31, 42, 994, 1068, 1706, 1896, 1996
BssSI	CACGAG	1 cut(s)	171
BssT1I	CCWWGG	1 cut(s)	258
Bst2BI	CACGAG	1 cut(s)	171
Bst2UI	CCWGG	2 cut(s)	90, 684
Bst4CI	ACNGT	4 cut(s)	284, 694, 1192, 1551
Bst6I	CTCTTC	1 cut(s)	1110
BstAPI	GCANNNNNTGC	3 cut(s)	1427, 1474, 1636
BstBI	TTCGAA	1 cut(s)	459
BstC8I	GCNNGC	6 cut(s)	555, 706, 1122, 1411, 1423, 2055
BstDEI	CTNAG	8 cut(s)	165, 192, 648, 866, 987, 1110, 1212, 1764
BstF5I	GGATG	8 cut(s)	74, 304, 602, 606, 895, 982, 1168, 1565
BstKTI	GATC	7 cut(s)	34, 45, 997, 1071, 1709, 1899, 1999
BstMAI	GTCTC	4 cut(s)	531, 574, 1647, 1890
BstMBI	GATC	7 cut(s)	31, 42, 994, 1068, 1706, 1896, 1996
BstMWI	GCNNNNNNNGC	9 cut(s)	166, 705, 758, 1307, 1410, 1427, 1465, 1474, 1636
BstNI	CCWGG	2 cut(s)	90, 684
BstNSI	RCATGY	3 cut(s)	1086, 1124, 2057
BstPAI	GACNNNNGTC	1 cut(s)	978
BstSCI	CCNGG	3 cut(s)	88, 682, 1771
BstSFI	CTRYAG	5 cut(s)	431, 873, 1204, 1320, 1849
BstV1I	GCAGC	4 cut(s)	147, 1480, 1487, 1702
BstV2I	GAAGAC	3 cut(s)	843, 1157, 1754
BstX2I	RGATCY	2 cut(s)	1068, 1706
BstXI	CCANNNNNNTGG	1 cut(s)	1793
BstYI	RGATCY	2 cut(s)	1068, 1706
BsuI	GTATCC	1 cut(s)	826
BsuRI	GGCC	4 cut(s)	213, 708, 752, 1845
BtsCI	GGATG	8 cut(s)	74, 304, 602, 606, 895, 982, 1168, 1565
BtsI	GCAGTG	3 cut(s)	186, 886, 1399
BtsIMutI	CAGTG	7 cut(s)	186, 459, 699, 886, 1399, 1547, 2007
BveI	ACCTGC	2 cut(s)	424, 1121
Cac8I	GCNNGC	6 cut(s)	555, 706, 1122, 1411, 1423, 2055
CaiI	CAGNNNCTG	1 cut(s)	1503
CciI	TCATGA	3 cut(s)	100, 1000, 2066
Cfr13I	GGNCC	2 cut(s)	392, 906
CseI	GACGC	1 cut(s)	1082
Csp6I	GTAC	4 cut(s)	58, 364, 673, 1571
CviAII	CATG	8 cut(s)	101, 509, 1001, 1083, 1121, 1805, 2054, 2067
CviQI	GTAC	4 cut(s)	58, 364, 673, 1571
DdeI	CTNAG	8 cut(s)	165, 192, 648, 866, 987, 1110, 1212, 1764
DpnI	GATC	7 cut(s)	33, 44, 996, 1070, 1708, 1898, 1998
DpnII	GATC	7 cut(s)	31, 42, 994, 1068, 1706, 1896, 1996
DraIII	CACNNNGTG	1 cut(s)	1405
EaeI	YGGCCR	2 cut(s)	211, 750
Eam1104I	CTCTTC	1 cut(s)	1110
EarI	CTCTTC	1 cut(s)	1110
EciI	GGCGGA	1 cut(s)	106
Ecl136II	GAGCTC	1 cut(s)	196
Eco130I	CCWWGG	1 cut(s)	258
Eco147I	AGGCCT	1 cut(s)	1845
Eco24I	GRGCYC	1 cut(s)	198
Eco47I	GGWCC	2 cut(s)	392, 906
Eco53kI	GAGCTC	1 cut(s)	196
Eco57I	CTGAAG	1 cut(s)	1646
EcoICRI	GAGCTC	1 cut(s)	196
EcoO109I	RGGNCCY	1 cut(s)	906
EcoRI	GAATTC	1 cut(s)	1968
EcoRII	CCWGG	2 cut(s)	88, 682
EcoT14I	CCWWGG	1 cut(s)	258
EcoT22I	ATGCAT	1 cut(s)	1513
EcoT38I	GRGCYC	1 cut(s)	198
ErhI	CCWWGG	1 cut(s)	258
FaeI	CATG	8 cut(s)	104, 512, 1004, 1086, 1124, 1808, 2057, 2070
FaqI	GGGAC	1 cut(s)	1932
FatI	CATG	8 cut(s)	100, 508, 1000, 1082, 1120, 1804, 2053, 2066
FauI	CCCGC	1 cut(s)	1696
FauNDI	CATATG	3 cut(s)	808, 1339, 1615
FbaI	TGATCA	1 cut(s)	31
Fnu4HI	GCNGC	6 cut(s)	161, 753, 1469, 1501, 1716, 2006
FokI	GGATG	8 cut(s)	61, 311, 609, 613, 902, 969, 1155, 1572
FriOI	GRGCYC	1 cut(s)	198
Fsp4HI	GCNGC	6 cut(s)	161, 753, 1469, 1501, 1716, 2006
FspBI	CTAG	3 cut(s)	732, 1452, 1685
GluI	GCNGC	6 cut(s)	161, 753, 1469, 1501, 1716, 2006
GsuI	CTGGAG	1 cut(s)	1164
HaeIII	GGCC	4 cut(s)	213, 708, 752, 1845
HapII	CCGG	1 cut(s)	1772
HgaI	GACGC	1 cut(s)	1082
Hin1II	CATG	8 cut(s)	104, 512, 1004, 1086, 1124, 1808, 2057, 2070
HpaII	CCGG	1 cut(s)	1772
HphI	GGTGA	2 cut(s)	617, 2016
Hpy166II	GTNNAC	1 cut(s)	1487
Hpy8I	GTNNAC	1 cut(s)	1487
Hpy99I	CGWCG	1 cut(s)	1098
HpyAV	CCTTC	6 cut(s)	48, 289, 320, 1525, 1820, 1826
HpyCH4III	ACNGT	4 cut(s)	284, 694, 1192, 1551
HpyF10VI	GCNNNNNNNGC	9 cut(s)	166, 705, 758, 1307, 1410, 1427, 1465, 1474, 1636
HpyF3I	CTNAG	8 cut(s)	165, 192, 648, 866, 987, 1110, 1212, 1764
Hsp92II	CATG	8 cut(s)	104, 512, 1004, 1086, 1124, 1808, 2057, 2070
KpnI	GGTACC	1 cut(s)	61
Ksp22I	TGATCA	1 cut(s)	31
Kzo9I	GATC	7 cut(s)	31, 42, 994, 1068, 1706, 1896, 1996
LguI	GCTCTTC	1 cut(s)	1110
LmnI	GCTCC	4 cut(s)	1315, 1355, 1565, 1892
Lsp1109I	GCAGC	4 cut(s)	147, 1480, 1487, 1702
LweI	GCATC	5 cut(s)	83, 1310, 1464, 1916, 2048
MaeI	CTAG	3 cut(s)	732, 1452, 1685
MaeIII	GTNAC	2 cut(s)	643, 785
MalI	GATC	7 cut(s)	33, 44, 996, 1070, 1708, 1898, 1998
MboI	GATC	7 cut(s)	31, 42, 994, 1068, 1706, 1896, 1996
MboII	GAAGA	8 cut(s)	116, 609, 843, 1097, 1157, 1759, 1891, 2022
MfeI	CAATTG	3 cut(s)	578, 1245, 1596
MflI	RGATCY	2 cut(s)	1068, 1706
MhlI	GDGCHC	2 cut(s)	188, 198
MlsI	TGGCCA	1 cut(s)	213
MluNI	TGGCCA	1 cut(s)	213
MlyI	GAGTC	4 cut(s)	938, 1756, 1855, 1954
MmeI	TCCRAC	2 cut(s)	1498, 1687
Mox20I	TGGCCA	1 cut(s)	213
Mph1103I	ATGCAT	1 cut(s)	1513
MroXI	GAANNNNTTC	1 cut(s)	478
MscI	TGGCCA	1 cut(s)	213
MseI	TTAA	4 cut(s)	135, 626, 1185, 1265
MslI	CAYNNNNRTG	3 cut(s)	27, 513, 1081
Msp20I	TGGCCA	1 cut(s)	213
MspA1I	CMGCKG	1 cut(s)	1500
MspI	CCGG	1 cut(s)	1772
MspR9I	CCNGG	3 cut(s)	90, 684, 1773
MunI	CAATTG	3 cut(s)	578, 1245, 1596
Mva1269I	GAATGC	3 cut(s)	678, 1061, 1513
MvaI	CCWGG	2 cut(s)	90, 684
MwoI	GCNNNNNNNGC	9 cut(s)	166, 705, 758, 1307, 1410, 1427, 1465, 1474, 1636
NciI	CCSGG	1 cut(s)	1773
NdeI	CATATG	3 cut(s)	808, 1339, 1615
NdeII	GATC	7 cut(s)	31, 42, 994, 1068, 1706, 1896, 1996
NlaIII	CATG	8 cut(s)	104, 512, 1004, 1086, 1124, 1808, 2057, 2070
NlaIV	GGNNCC	4 cut(s)	59, 342, 908, 1016
NsiI	ATGCAT	1 cut(s)	1513
NspI	RCATGY	3 cut(s)	1086, 1124, 2057
NspV	TTCGAA	1 cut(s)	459
PaeI	GCATGC	2 cut(s)	1124, 2057
PagI	TCATGA	3 cut(s)	100, 1000, 2066
PaqCI	CACCTGC	1 cut(s)	1121
PceI	AGGCCT	1 cut(s)	1845
PciSI	GCTCTTC	1 cut(s)	1110
PctI	GAATGC	3 cut(s)	678, 1061, 1513
PdmI	GAANNNNTTC	1 cut(s)	478
PfeI	GAWTC	6 cut(s)	107, 533, 1343, 1521, 1768, 1801
PflMI	CCANNNNNTGG	1 cut(s)	1405
PfoI	TCCNGGA	1 cut(s)	682
PkrI	GCNGC	6 cut(s)	162, 754, 1470, 1502, 1717, 2007
PleI	GAGTC	4 cut(s)	938, 1756, 1855, 1953
PpsI	GAGTC	4 cut(s)	938, 1756, 1855, 1953
PpuMI	RGGWCCY	1 cut(s)	906
PshAI	GACNNNNGTC	1 cut(s)	978
Psp124BI	GAGCTC	1 cut(s)	198
Psp5II	RGGWCCY	1 cut(s)	906
Psp6I	CCWGG	2 cut(s)	88, 682
PspGI	CCWGG	2 cut(s)	88, 682
PspN4I	GGNNCC	4 cut(s)	59, 342, 908, 1016
PspPI	GGNCC	2 cut(s)	392, 906
PspPPI	RGGWCCY	1 cut(s)	906
PstI	CTGCAG	3 cut(s)	435, 1208, 1324
PstNI	CAGNNNCTG	1 cut(s)	1503
PsuI	RGATCY	2 cut(s)	1068, 1706
PvuII	CAGCTG	1 cut(s)	1500
RsaI	GTAC	4 cut(s)	59, 365, 674, 1572
RsaNI	GTAC	4 cut(s)	58, 364, 673, 1571
RseI	CAYNNNNRTG	3 cut(s)	27, 513, 1081
SacI	GAGCTC	1 cut(s)	198
SapI	GCTCTTC	1 cut(s)	1110
SaqAI	TTAA	4 cut(s)	135, 626, 1185, 1265
SatI	GCNGC	6 cut(s)	161, 753, 1469, 1501, 1716, 2006
Sau3AI	GATC	7 cut(s)	31, 42, 994, 1068, 1706, 1896, 1996
Sau96I	GGNCC	2 cut(s)	392, 906
ScaI	AGTACT	2 cut(s)	365, 674
SchI	GAGTC	4 cut(s)	938, 1756, 1855, 1954
ScrFI	CCNGG	3 cut(s)	90, 684, 1773
SduI	GDGCHC	2 cut(s)	188, 198
SfaNI	GCATC	5 cut(s)	83, 1310, 1464, 1916, 2048
SfcI	CTRYAG	5 cut(s)	431, 873, 1204, 1320, 1849
SfuI	TTCGAA	1 cut(s)	459
SinI	GGWCC	2 cut(s)	392, 906
SmiMI	CAYNNNNRTG	3 cut(s)	27, 513, 1081
SmlI	CTYRAG	2 cut(s)	528, 1887
SmoI	CTYRAG	2 cut(s)	528, 1887
SpeI	ACTAGT	2 cut(s)	731, 1684
SphI	GCATGC	2 cut(s)	1124, 2057
SseBI	AGGCCT	1 cut(s)	1845
SsiI	CCGC	5 cut(s)	117, 753, 1413, 1703, 2006
SspMI	CTAG	3 cut(s)	732, 1452, 1685
SstI	GAGCTC	1 cut(s)	198
StuI	AGGCCT	1 cut(s)	1845
StyD4I	CCNGG	3 cut(s)	88, 682, 1771
StyI	CCWWGG	1 cut(s)	258
TaaI	ACNGT	4 cut(s)	284, 694, 1192, 1551
TaqI	TCGA	4 cut(s)	41, 416, 459, 947
TatI	WGTACW	3 cut(s)	363, 672, 1570
TauI	GCSGC	2 cut(s)	755, 2008
TfiI	GAWTC	6 cut(s)	107, 533, 1343, 1521, 1768, 1801
Tru1I	TTAA	4 cut(s)	135, 626, 1185, 1265
Tru9I	TTAA	4 cut(s)	135, 626, 1185, 1265
TscAI	CASTG	7 cut(s)	193, 459, 699, 886, 1406, 1554, 2007
TseI	GCWGC	4 cut(s)	160, 1468, 1500, 1715
TspRI	CASTG	7 cut(s)	193, 459, 699, 886, 1406, 1554, 2007
Van91I	CCANNNNNTGG	1 cut(s)	1405
VpaK11BI	GGWCC	2 cut(s)	392, 906
XapI	RAATTY	3 cut(s)	385, 1916, 1968
XceI	RCATGY	3 cut(s)	1086, 1124, 2057
XcmI	CCANNNNNNNNNTGG	1 cut(s)	281
XmnI	GAANNNNTTC	1 cut(s)	478
XspI	CTAG	3 cut(s)	732, 1452, 1685
ZrmI	AGTACT	2 cut(s)	365, 674
Zsp2I	ATGCAT	1 cut(s)	1513

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.