MD06G1089500.v1.1

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008937
Physical Location & Seq
Reverse (-)
21793744 .. 21797906
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1089500.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGCTGCCAAGGAGTCGAGCTCTGCAGCTGCTTCGGCGGACGGAAAAATCAAAAGGGTGCTCACCCATGGCGGTAGATATGCGCAGTACAATGTGTTTGGGAACTTGTTCGAGGTCTCCAGCAAGTACGTGCCTCCCATTAGGCCTATTGGGAGAGGTGCTTATGGCATTGTCTGTGCGGCTGTTAATTCAGACACCCATGAGGAAGTTGCAATTAAGAAGATTGGTAACGCTTTTGACAATATAATAGATGCCAAAAGGACACTAAGAGAAATCAAGCTTCTTCGTCACATGGACCATGAAAATGTTATTTCTATCAAGGACATTGTACGACCACCCAAAAAAGAGACTTTCAATGACGTCTACGTTGTTTATGAATTGATGGACACTGATCTTCATCAGATCATTCGTTCTGACCAACCATTGACAGATGATCATTGTCAGTACTTTCTGTATCAGCTGTTACGAGGACTAAAATATGTGCACTCAGCCAATGTCTTGCACCGTGATTTGAAGCCAAGTAATCTGCTTCTAAATGCTAACTGTGACCTTAAAATTGGAGATTTTGGACTAGCAAGAACAACATCTGAGACAGATTTCATGACCGAGTATGTTGTCACTCGTTGGTACCGAGCACCAGAGTTGCTACTTAATTGTTCAGAGTACACAGCTGCAATTGATATCTGGTCTGTTGGTTGCATACTTGGTGAAATCATGACGAGAGAACCTTTGTTTCCTGGGAAAGATTATGTCCATCAGCTGAGGCTTATTACGGAGTTGATAGGTTCACCTGATGATGCGAGCCTCGGATTTCTTCGAAGTGATAATGCCCGTAGATATGCAAAACAGCTTCCACAATTCCGGAGGCAACAATTCGCTGCTAGATTCCCTAATATGTCTCCTGGGGCTGTGGATTTGCTGGAGAAAATGCTTGTGTTCGATCCTAGTAGACGCATTACTGTCGACGAGGCACTTTGTCACCCATACTTGTTGTCCCTTCATGATAACAATGATGAGCCCATCTGCGCAAGGCCTTTCCATTTTGATTTTGAGCAACCTTCGTGCACCGAAGAGCACATCAAAGAGCTCATCTGGAGAGAAGCAGTGAAGTTCAATCCAGACCCAACCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006971 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009504 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009861 GO:0009862 GO:0009863 GO:0009867 GO:0009868 GO:0009987 GO:0010033 GO:0010468 GO:0014070 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031122 GO:0032260 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032870 GO:0033206 GO:0033993 GO:0035556 GO:0035690 GO:0035821 GO:0036211 GO:0042221 GO:0042493 GO:0042539 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0046677 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051321 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052306 GO:0052308 GO:0052552 GO:0052553 GO:0052564 GO:0052572 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071840 GO:0075136 GO:0097305 GO:0097435 GO:0098542 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902410 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04016 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04138 ko04139 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05206 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 ko05418 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04016 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04138 map04139 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05206 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

378

Amino Acids

43.08

Weight (kDa)

6.17

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 48 - 330 3e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 48 - 241 2e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 363
Acc16I TGCGCA 2 cut(s) 84, 1027
Acc65I GGTACC 1 cut(s) 627
AccB1I GGYRCC 1 cut(s) 627
AccI GTMKAC 3 cut(s) 363, 949, 963
AccIII TCCGGA 1 cut(s) 861
AciI CCGC 3 cut(s) 38, 72, 179
AclWI GGATC 1 cut(s) 935
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 6 cut(s) 89, 128, 330, 446, 629, 665
AgsI TTSAA 3 cut(s) 355, 514, 1114
AjnI CCWGG 2 cut(s) 736, 901
AluBI AGCT 8 cut(s) 21, 29, 280, 460, 671, 760, 850, 1087
AluI AGCT 8 cut(s) 21, 29, 280, 460, 671, 760, 850, 1087
Alw21I GWGCWC 7 cut(s) 23, 63, 486, 637, 1067, 1077, 1089
Alw26I GTCTC 4 cut(s) 121, 341, 584, 903
Alw44I GTGCAC 2 cut(s) 482, 1063
AlwI GGATC 1 cut(s) 935
Aor13HI TCCGGA 1 cut(s) 861
AoxI GGCC 2 cut(s) 143, 1031
ApaLI GTGCAC 2 cut(s) 482, 1063
ApeKI GCWGC 5 cut(s) 5, 26, 29, 671, 878
Asp700I GAANNNNTTC 1 cut(s) 107
Asp718I GGTACC 1 cut(s) 627
AspLEI GCGC 2 cut(s) 85, 1028
AspS9I GGNCC 1 cut(s) 295
AsuHPI GGTGA 4 cut(s) 55, 719, 780, 971
AsuII TTCGAA 1 cut(s) 817
AvaII GGWCC 1 cut(s) 295
BaeGI GKGCMC 2 cut(s) 486, 1067
BanI GGYRCC 1 cut(s) 627
BanII GRGCYC 3 cut(s) 23, 1020, 1089
Bbv12I GWGCWC 7 cut(s) 23, 63, 486, 637, 1067, 1077, 1089
BbvCI CCTCAGC 1 cut(s) 761
BbvI GCAGC 4 cut(s) 16, 38, 658, 865
BccI CCATC 3 cut(s) 376, 762, 1028
BcgI CGANNNNNNTGC 4 cut(s) 15, 49, 943, 977
BciT130I CCWGG 2 cut(s) 738, 903
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 4 cut(s) 121, 341, 584, 903
BfaI CTAG 3 cut(s) 572, 882, 945
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 6 cut(s) 6, 27, 30, 180, 672, 879
BlsI GCNGC 6 cut(s) 7, 28, 31, 181, 673, 880
BmcAI AGTACT 1 cut(s) 446
Bme1390I CCNGG 2 cut(s) 738, 903
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 629
BmrFI CCNGG 2 cut(s) 738, 903
BmsI GCATC 2 cut(s) 241, 787
BplI GAGNNNNNCTC 1 cut(s) 37
BpmI CTGGAG 3 cut(s) 103, 941, 1114
Bpu10I CCTNAGC 1 cut(s) 761
Bpu14I TTCGAA 1 cut(s) 817
BsaAI YACGTR 1 cut(s) 130
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 5 cut(s) 9, 67, 737, 805, 902
BsaWI WCCGGW 1 cut(s) 861
Bse8I GATNNNNATC 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 861
BseBI CCWGG 2 cut(s) 738, 903
BseDI CCNNGG 5 cut(s) 9, 67, 737, 805, 902
BseJI GATNNNNATC 1 cut(s) 396
BseMII CTCAG 3 cut(s) 501, 579, 752
BseSI GKGCMC 2 cut(s) 486, 1067
BseXI GCAGC 4 cut(s) 16, 38, 658, 865
BshFI GGCC 2 cut(s) 145, 1033
BshNI GGYRCC 1 cut(s) 627
BsiHKAI GWGCWC 7 cut(s) 23, 63, 486, 637, 1067, 1077, 1089
BsiSI CCGG 1 cut(s) 862
BslFI GGGAC 1 cut(s) 979
BsmAI GTCTC 4 cut(s) 121, 341, 584, 903
BsmFI GGGAC 1 cut(s) 979
BsnI GGCC 2 cut(s) 145, 1033
Bso31I GGTCTC 1 cut(s) 121
Bsp119I TTCGAA 1 cut(s) 817
Bsp1286I GDGCHC 8 cut(s) 23, 63, 486, 637, 1020, 1067, 1077, 1089
Bsp13I TCCGGA 1 cut(s) 861
Bsp143I GATC 4 cut(s) 391, 402, 433, 940
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 3 cut(s) 38, 72, 179
BspANI GGCC 2 cut(s) 145, 1033
BspCNI CTCAG 3 cut(s) 500, 580, 753
BspEI TCCGGA 1 cut(s) 861
BspHI TCATGA 3 cut(s) 600, 714, 1000
BspLI GGNNCC 1 cut(s) 629
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 1 cut(s) 935
BspQI GCTCTTC 1 cut(s) 1065
BspT104I TTCGAA 1 cut(s) 817
BspT107I GGYRCC 1 cut(s) 627
BspTNI GGTCTC 1 cut(s) 121
BssECI CCNNGG 5 cut(s) 9, 67, 737, 805, 902
BssMI GATC 4 cut(s) 391, 402, 433, 940
BssNI GRCGYC 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 9, 67
Bst2UI CCWGG 2 cut(s) 738, 903
Bst4CI ACNGT 3 cut(s) 506, 545, 961
Bst6I CTCTTC 1 cut(s) 1065
BstACI GRCGYC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 130
BstBI TTCGAA 1 cut(s) 817
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 4 cut(s) 266, 487, 588, 761
BstDSI CCRYGG 1 cut(s) 67
BstHHI GCGC 2 cut(s) 85, 1028
BstKTI GATC 4 cut(s) 394, 405, 436, 943
BstMAI GTCTC 4 cut(s) 121, 341, 584, 903
BstMBI GATC 4 cut(s) 391, 402, 433, 940
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstNI CCWGG 2 cut(s) 738, 903
BstSCI CCNGG 2 cut(s) 736, 901
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 2 cut(s) 486, 1067
BstV1I GCAGC 4 cut(s) 16, 38, 658, 865
BsuRI GGCC 2 cut(s) 145, 1033
BtgI CCRYGG 1 cut(s) 67
BtsI GCAGTG 1 cut(s) 1110
BtsIMutI CAGTG 2 cut(s) 387, 1110
Cac8I GCNNGC 1 cut(s) 802
CciI TCATGA 3 cut(s) 600, 714, 1000
CfoI GCGC 2 cut(s) 85, 1028
Cfr13I GGNCC 1 cut(s) 295
CseI GACGC 1 cut(s) 960
Csp6I GTAC 6 cut(s) 88, 127, 329, 445, 628, 664
CviAII CATG 7 cut(s) 68, 200, 292, 299, 601, 715, 1001
CviQI GTAC 6 cut(s) 88, 127, 329, 445, 628, 664
DdeI CTNAG 4 cut(s) 266, 487, 588, 761
DpnI GATC 4 cut(s) 393, 404, 435, 942
DpnII GATC 4 cut(s) 391, 402, 433, 940
Eam1104I CTCTTC 1 cut(s) 1065
EarI CTCTTC 1 cut(s) 1065
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 2 cut(s) 21, 1087
Eco130I CCWWGG 2 cut(s) 9, 67
Eco147I AGGCCT 2 cut(s) 145, 1033
Eco24I GRGCYC 3 cut(s) 23, 1020, 1089
Eco31I GGTCTC 1 cut(s) 121
Eco32I GATATC 1 cut(s) 682
Eco47I GGWCC 1 cut(s) 295
Eco53kI GAGCTC 2 cut(s) 21, 1087
EcoICRI GAGCTC 2 cut(s) 21, 1087
EcoRII CCWGG 2 cut(s) 736, 901
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 2 cut(s) 9, 67
EcoT38I GRGCYC 3 cut(s) 23, 1020, 1089
ErhI CCWWGG 2 cut(s) 9, 67
FaeI CATG 7 cut(s) 71, 203, 295, 302, 604, 718, 1004
FaqI GGGAC 1 cut(s) 979
FatI CATG 7 cut(s) 67, 199, 291, 298, 600, 714, 1000
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 3 cut(s) 363, 949, 963
Fnu4HI GCNGC 6 cut(s) 6, 27, 30, 180, 672, 879
FriOI GRGCYC 3 cut(s) 23, 1020, 1089
Fsp4HI GCNGC 6 cut(s) 6, 27, 30, 180, 672, 879
FspBI CTAG 3 cut(s) 572, 882, 945
FspI TGCGCA 2 cut(s) 84, 1027
GlaI GCGC 2 cut(s) 84, 1027
GluI GCNGC 6 cut(s) 6, 27, 30, 180, 672, 879
GsuI CTGGAG 3 cut(s) 103, 941, 1114
HaeIII GGCC 2 cut(s) 145, 1033
HapII CCGG 1 cut(s) 862
HgaI GACGC 1 cut(s) 960
HhaI GCGC 2 cut(s) 85, 1028
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 7 cut(s) 71, 203, 295, 302, 604, 718, 1004
Hin6I GCGC 2 cut(s) 83, 1026
HinP1I GCGC 2 cut(s) 83, 1026
HincII GTYRAC 1 cut(s) 964
HindII GTYRAC 1 cut(s) 964
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 2 cut(s) 14, 885
HpaII CCGG 1 cut(s) 862
HphI GGTGA 4 cut(s) 55, 719, 780, 971
Hpy166II GTNNAC 7 cut(s) 364, 484, 666, 788, 950, 964, 1065
Hpy188I TCNGA 6 cut(s) 193, 402, 415, 589, 661, 809
Hpy188III TCNNGA 6 cut(s) 601, 715, 862, 1001, 1093, 1118
Hpy8I GTNNAC 7 cut(s) 364, 484, 666, 788, 950, 964, 1065
Hpy99I CGWCG 1 cut(s) 968
HpyAV CCTTC 2 cut(s) 1007, 1068
HpyCH4III ACNGT 3 cut(s) 506, 545, 961
HpyCH4IV ACGT 3 cut(s) 129, 360, 366
HpyCH4V TGCA 8 cut(s) 26, 212, 484, 502, 674, 699, 842, 1065
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpyF3I CTNAG 4 cut(s) 266, 487, 588, 761
HpySE526I ACGT 3 cut(s) 129, 360, 366
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 7 cut(s) 71, 203, 295, 302, 604, 718, 1004
HspAI GCGC 2 cut(s) 83, 1026
Kpn2I TCCGGA 1 cut(s) 861
KpnI GGTACC 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 4 cut(s) 391, 402, 433, 940
LguI GCTCTTC 1 cut(s) 1065
Lsp1109I GCAGC 4 cut(s) 16, 38, 658, 865
LweI GCATC 2 cut(s) 241, 787
MaeI CTAG 3 cut(s) 572, 882, 945
MaeII ACGT 3 cut(s) 129, 360, 366
MaeIII GTNAC 6 cut(s) 227, 287, 462, 545, 616, 977
MalI GATC 4 cut(s) 393, 404, 435, 942
MboI GATC 4 cut(s) 391, 402, 433, 940
MboII GAAGA 5 cut(s) 232, 275, 386, 806, 1082
MfeI CAATTG 1 cut(s) 675
MhlI GDGCHC 8 cut(s) 23, 63, 486, 637, 1020, 1067, 1077, 1089
MluCI AATT 8 cut(s) 187, 213, 377, 555, 652, 675, 857, 872
MlyI GAGTC 1 cut(s) 23
MnlI CCTC 9 cut(s) 106, 144, 149, 196, 461, 756, 815, 858, 961
MroI TCCGGA 1 cut(s) 861
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 5 cut(s) 186, 216, 552, 651, 1132
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 4 cut(s) 29, 460, 671, 760
MspI CCGG 1 cut(s) 862
MspR9I CCNGG 2 cut(s) 738, 903
MunI CAATTG 1 cut(s) 675
MvaI CCWGG 2 cut(s) 738, 903
MwoI GCNNNNNNNGC 1 cut(s) 35
NcoI CCATGG 1 cut(s) 67
NdeII GATC 4 cut(s) 391, 402, 433, 940
NlaIII CATG 7 cut(s) 71, 203, 295, 302, 604, 718, 1004
NlaIV GGNNCC 1 cut(s) 629
NmuCI GTSAC 4 cut(s) 287, 545, 616, 977
NsbI TGCGCA 2 cut(s) 84, 1027
NspV TTCGAA 1 cut(s) 817
PagI TCATGA 3 cut(s) 600, 714, 1000
PceI AGGCCT 2 cut(s) 145, 1033
PciSI GCTCTTC 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 885
PkrI GCNGC 6 cut(s) 7, 28, 31, 181, 673, 880
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Ppu21I YACGTR 1 cut(s) 130
Psp124BI GAGCTC 2 cut(s) 23, 1089
Psp6I CCWGG 2 cut(s) 736, 901
PspGI CCWGG 2 cut(s) 736, 901
PspN4I GGNNCC 1 cut(s) 629
PspPI GGNCC 1 cut(s) 295
PstI CTGCAG 1 cut(s) 28
PvuII CAGCTG 4 cut(s) 29, 460, 671, 760
RsaI GTAC 6 cut(s) 89, 128, 330, 446, 629, 665
RsaNI GTAC 6 cut(s) 88, 127, 329, 445, 628, 664
RseI CAYNNNNRTG 1 cut(s) 303
SacI GAGCTC 2 cut(s) 23, 1089
SalI GTCGAC 1 cut(s) 962
SapI GCTCTTC 1 cut(s) 1065
SaqAI TTAA 5 cut(s) 186, 216, 552, 651, 1132
SatI GCNGC 6 cut(s) 6, 27, 30, 180, 672, 879
Sau3AI GATC 4 cut(s) 391, 402, 433, 940
Sau96I GGNCC 1 cut(s) 295
ScaI AGTACT 1 cut(s) 446
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 2 cut(s) 738, 903
SduI GDGCHC 8 cut(s) 23, 63, 486, 637, 1020, 1067, 1077, 1089
SfaNI GCATC 2 cut(s) 241, 787
SfcI CTRYAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 817
SinI GGWCC 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 303
Sse9I AATT 8 cut(s) 187, 213, 377, 555, 652, 675, 857, 872
SseBI AGGCCT 2 cut(s) 145, 1033
SsiI CCGC 3 cut(s) 38, 72, 179
SspMI CTAG 3 cut(s) 572, 882, 945
SstI GAGCTC 2 cut(s) 23, 1089
StuI AGGCCT 2 cut(s) 145, 1033
StyD4I CCNGG 2 cut(s) 736, 901
StyI CCWWGG 2 cut(s) 9, 67
TaaI ACNGT 3 cut(s) 506, 545, 961
TaiI ACGT 3 cut(s) 132, 363, 369
TaqI TCGA 5 cut(s) 17, 111, 817, 939, 963
TaqII GACCGA 1 cut(s) 620
TasI AATT 8 cut(s) 187, 213, 377, 555, 652, 675, 857, 872
TatI WGTACW 3 cut(s) 87, 444, 663
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 1 cut(s) 885
Tru1I TTAA 5 cut(s) 186, 216, 552, 651, 1132
Tru9I TTAA 5 cut(s) 186, 216, 552, 651, 1132
TscAI CASTG 2 cut(s) 394, 1110
TseFI GTSAC 4 cut(s) 287, 545, 616, 977
TseI GCWGC 5 cut(s) 5, 26, 29, 671, 878
Tsp45I GTSAC 4 cut(s) 287, 545, 616, 977
TspDTI ATGAA 5 cut(s) 315, 386, 390, 589, 989
TspGWI ACGGA 2 cut(s) 57, 788
TspRI CASTG 2 cut(s) 394, 1110
VneI GTGCAC 2 cut(s) 482, 1063
VpaK11BI GGWCC 1 cut(s) 295
XmiI GTMKAC 3 cut(s) 363, 949, 963
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 3 cut(s) 572, 882, 945
ZraI GACGTC 1 cut(s) 361
ZrmI AGTACT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.