pycom06g08540

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008937
Physical Location & Seq
Reverse (-)
13767340 .. 13768411
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08540.1

Sequence Viewer

Length: 534 bp
ATGACCGAGTATGTTGTCACTCGTTGGTACCGAGCACCAGAGTTGCTACTTAATTGTTCAGAGTACACAGCTGCAATTGATATCTGGTCTGTTGGTTGCATACTTGGTGAAATCATGACGAGAGAACCTTTGTTTCCTGGGAAAGACTATGTCCATCAGCTGAGGCTTATTACGGAGTTGATAGGTTCACCTGATGATGCGAGCCTCGGATTTCTTCGAAGTGATAATGCCCGAAGATATGCAAAACAGCTTCCACAATTCCGGAGGCAACAATTTGCTGCTAGATTCCCTAATATGTCTCCTGGGGCTGTGGATTTGCTGGAGAAAATGCTTGTGTTTGATCCTAGTAGACGCATTACTGTTGACGAGGCACTTTGTCACCCATACTTGTCGTCCCTTCATGATAACAATGATGAGCCCATCTGCGCAAGGCCTTTCCATTTTGATTTTGAGCAACCTTCGTGCACTGAAGAGCACATCAAAGAGCTCATCTGGAGAGAAGCAGTGAAGTTCAATCCAGACCCAACCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006971 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009504 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009861 GO:0009862 GO:0009863 GO:0009867 GO:0009868 GO:0009987 GO:0010033 GO:0010468 GO:0014070 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031122 GO:0032260 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032870 GO:0033206 GO:0033993 GO:0035556 GO:0035690 GO:0035821 GO:0036211 GO:0042221 GO:0042493 GO:0042539 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0046677 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051321 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052306 GO:0052308 GO:0052552 GO:0052553 GO:0052564 GO:0052572 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071840 GO:0075136 GO:0097305 GO:0097435 GO:0098542 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902410 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04016 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04138 ko04139 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05206 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 ko05418 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04016 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04138 map04139 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05206 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

178

Amino Acids

20.56

Weight (kDa)

5.35

Isoelectric Point (pI)

66.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 130 6.7e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 427
Acc65I GGTACC 1 cut(s) 27
AccB1I GGYRCC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 349
AccIII TCCGGA 1 cut(s) 261
AclWI GGATC 1 cut(s) 335
AcuI CTGAAG 1 cut(s) 489
AfaI GTAC 2 cut(s) 29, 65
AgsI TTSAA 1 cut(s) 514
AjnI CCWGG 2 cut(s) 136, 301
AluBI AGCT 4 cut(s) 71, 160, 250, 487
AluI AGCT 4 cut(s) 71, 160, 250, 487
Alw21I GWGCWC 4 cut(s) 37, 467, 477, 489
Alw26I GTCTC 1 cut(s) 303
Alw44I GTGCAC 1 cut(s) 463
AlwI GGATC 1 cut(s) 335
Aor13HI TCCGGA 1 cut(s) 261
AoxI GGCC 1 cut(s) 431
ApaLI GTGCAC 1 cut(s) 463
ApeKI GCWGC 2 cut(s) 71, 278
Asp718I GGTACC 1 cut(s) 27
AspLEI GCGC 1 cut(s) 428
AsuHPI GGTGA 3 cut(s) 119, 180, 371
AsuII TTCGAA 1 cut(s) 217
BaeGI GKGCMC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 27
BanII GRGCYC 2 cut(s) 420, 489
Bbv12I GWGCWC 4 cut(s) 37, 467, 477, 489
BbvCI CCTCAGC 1 cut(s) 161
BbvI GCAGC 2 cut(s) 58, 265
BccI CCATC 2 cut(s) 162, 428
BciT130I CCWGG 2 cut(s) 138, 303
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 2 cut(s) 282, 345
BisI GCNGC 2 cut(s) 72, 279
BlsI GCNGC 2 cut(s) 73, 280
Bme1390I CCNGG 2 cut(s) 138, 303
BmiI GGNNCC 1 cut(s) 29
BmrFI CCNGG 2 cut(s) 138, 303
BmsI GCATC 1 cut(s) 187
BpmI CTGGAG 2 cut(s) 341, 514
Bpu10I CCTNAGC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 137, 205, 302
BsaWI WCCGGW 1 cut(s) 261
BseAI TCCGGA 1 cut(s) 261
BseBI CCWGG 2 cut(s) 138, 303
BseDI CCNNGG 3 cut(s) 137, 205, 302
BseMII CTCAG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 467
BseXI GCAGC 2 cut(s) 58, 265
BshFI GGCC 1 cut(s) 433
BshNI GGYRCC 1 cut(s) 27
BsiHKAI GWGCWC 4 cut(s) 37, 467, 477, 489
BsiSI CCGG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 379
BsmAI GTCTC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 379
BsnI GGCC 1 cut(s) 433
Bsp119I TTCGAA 1 cut(s) 217
Bsp1286I GDGCHC 5 cut(s) 37, 420, 467, 477, 489
Bsp13I TCCGGA 1 cut(s) 261
Bsp143I GATC 1 cut(s) 340
BspANI GGCC 1 cut(s) 433
BspCNI CTCAG 1 cut(s) 153
BspEI TCCGGA 1 cut(s) 261
BspHI TCATGA 2 cut(s) 114, 400
BspLI GGNNCC 1 cut(s) 29
BspPI GGATC 1 cut(s) 335
BspQI GCTCTTC 1 cut(s) 465
BspT104I TTCGAA 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 27
BssECI CCNNGG 3 cut(s) 137, 205, 302
BssMI GATC 1 cut(s) 340
Bst2UI CCWGG 2 cut(s) 138, 303
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 1 cut(s) 465
BstBI TTCGAA 1 cut(s) 217
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 1 cut(s) 340
BstNI CCWGG 2 cut(s) 138, 303
BstSCI CCNGG 2 cut(s) 136, 301
BstSLI GKGCMC 1 cut(s) 467
BstV1I GCAGC 2 cut(s) 58, 265
BsuRI GGCC 1 cut(s) 433
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 2 cut(s) 465, 510
Cac8I GCNNGC 1 cut(s) 202
CciI TCATGA 2 cut(s) 114, 400
CfoI GCGC 1 cut(s) 428
CseI GACGC 1 cut(s) 360
Csp6I GTAC 2 cut(s) 28, 64
CviAII CATG 2 cut(s) 115, 401
CviJI RGCY 9 cut(s) 71, 160, 166, 204, 250, 308, 418, 433, 487
CviKI_1 RGCY 9 cut(s) 71, 160, 166, 204, 250, 308, 418, 433, 487
CviQI GTAC 2 cut(s) 28, 64
DdeI CTNAG 1 cut(s) 161
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 487
Eco147I AGGCCT 1 cut(s) 433
Eco24I GRGCYC 2 cut(s) 420, 489
Eco32I GATATC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 489
EcoICRI GAGCTC 1 cut(s) 487
EcoRII CCWGG 2 cut(s) 136, 301
EcoRV GATATC 1 cut(s) 82
EcoT38I GRGCYC 2 cut(s) 420, 489
FaeI CATG 2 cut(s) 118, 404
FaiI YATR 8 cut(s) 12, 101, 116, 150, 240, 296, 385, 402
FaqI GGGAC 1 cut(s) 379
FatI CATG 2 cut(s) 114, 400
FblI GTMKAC 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 72, 279
FriOI GRGCYC 2 cut(s) 420, 489
Fsp4HI GCNGC 2 cut(s) 72, 279
FspBI CTAG 2 cut(s) 282, 345
FspI TGCGCA 1 cut(s) 427
GlaI GCGC 1 cut(s) 427
GluI GCNGC 2 cut(s) 72, 279
GsuI CTGGAG 2 cut(s) 341, 514
HaeIII GGCC 1 cut(s) 433
HapII CCGG 1 cut(s) 262
HgaI GACGC 1 cut(s) 360
HhaI GCGC 1 cut(s) 428
Hin1II CATG 2 cut(s) 118, 404
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 1 cut(s) 285
HpaII CCGG 1 cut(s) 262
HphI GGTGA 3 cut(s) 119, 180, 371
Hpy166II GTNNAC 5 cut(s) 66, 188, 350, 364, 465
Hpy188I TCNGA 2 cut(s) 61, 209
Hpy188III TCNNGA 5 cut(s) 115, 262, 401, 493, 518
Hpy8I GTNNAC 5 cut(s) 66, 188, 350, 364, 465
HpyAV CCTTC 2 cut(s) 407, 468
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4V TGCA 4 cut(s) 74, 99, 242, 465
HpyF3I CTNAG 1 cut(s) 161
Hsp92II CATG 2 cut(s) 118, 404
HspAI GCGC 1 cut(s) 426
Kpn2I TCCGGA 1 cut(s) 261
KpnI GGTACC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 340
LguI GCTCTTC 1 cut(s) 465
Lsp1109I GCAGC 2 cut(s) 58, 265
LweI GCATC 1 cut(s) 187
MaeI CTAG 2 cut(s) 282, 345
MaeIII GTNAC 2 cut(s) 16, 377
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 3 cut(s) 206, 246, 482
MfeI CAATTG 1 cut(s) 75
MhlI GDGCHC 5 cut(s) 37, 420, 467, 477, 489
MluCI AATT 4 cut(s) 52, 75, 257, 272
MnlI CCTC 4 cut(s) 156, 215, 258, 361
MroI TCCGGA 1 cut(s) 261
MseI TTAA 2 cut(s) 51, 532
MspA1I CMGCKG 2 cut(s) 71, 160
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 2 cut(s) 138, 303
MunI CAATTG 1 cut(s) 75
MvaI CCWGG 2 cut(s) 138, 303
NdeII GATC 1 cut(s) 340
NlaIII CATG 2 cut(s) 118, 404
NlaIV GGNNCC 1 cut(s) 29
NmuCI GTSAC 2 cut(s) 16, 377
NsbI TGCGCA 1 cut(s) 427
NspV TTCGAA 1 cut(s) 217
PagI TCATGA 2 cut(s) 114, 400
PceI AGGCCT 1 cut(s) 433
PciSI GCTCTTC 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PfeI GAWTC 1 cut(s) 285
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 2 cut(s) 73, 280
Psp124BI GAGCTC 1 cut(s) 489
Psp6I CCWGG 2 cut(s) 136, 301
PspGI CCWGG 2 cut(s) 136, 301
PspN4I GGNNCC 1 cut(s) 29
PsyI GACNNNGTC 1 cut(s) 149
PvuII CAGCTG 2 cut(s) 71, 160
RsaI GTAC 2 cut(s) 29, 65
RsaNI GTAC 2 cut(s) 28, 64
SacI GAGCTC 1 cut(s) 489
SapI GCTCTTC 1 cut(s) 465
SaqAI TTAA 2 cut(s) 51, 532
SatI GCNGC 2 cut(s) 72, 279
Sau3AI GATC 1 cut(s) 340
ScrFI CCNGG 2 cut(s) 138, 303
SduI GDGCHC 5 cut(s) 37, 420, 467, 477, 489
SetI ASST 8 cut(s) 73, 130, 162, 187, 193, 252, 460, 489
SfaNI GCATC 1 cut(s) 187
SfuI TTCGAA 1 cut(s) 217
Sse9I AATT 4 cut(s) 52, 75, 257, 272
SseBI AGGCCT 1 cut(s) 433
SspMI CTAG 2 cut(s) 282, 345
SstI GAGCTC 1 cut(s) 489
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 2 cut(s) 136, 301
TaaI ACNGT 1 cut(s) 361
TaqI TCGA 1 cut(s) 217
TaqII GACCGA 1 cut(s) 20
TasI AATT 4 cut(s) 52, 75, 257, 272
TatI WGTACW 1 cut(s) 63
TfiI GAWTC 1 cut(s) 285
Tru1I TTAA 2 cut(s) 51, 532
Tru9I TTAA 2 cut(s) 51, 532
TscAI CASTG 2 cut(s) 472, 510
TseFI GTSAC 2 cut(s) 16, 377
TseI GCWGC 2 cut(s) 71, 278
Tsp45I GTSAC 2 cut(s) 16, 377
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 2 cut(s) 472, 510
Tth111I GACNNNGTC 1 cut(s) 149
VneI GTGCAC 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 349
XspI CTAG 2 cut(s) 282, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.