MD06G1177700.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016842
Physical Location & Seq
Reverse (-)
31806756 .. 31808872
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1177700.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGGATCGGATTTCCATTTGGGCACTCGCCATTTTGATCATTCTCGTTATCATTCCAATGGCGGATTCAGAAGATCAAGAGTGGACTCAGATCAAGCAAGCCCTGGTGCAACTCATGGACAAACTCTCACCGGCCAATGCAACGTCCAGAGACGGAAATTGGGGTTGGAACATGAGCTCGGATCCTTGCATAGATAAATGGGAAGGTGTGACGTGTGACAGAAAGAATTCTGTCAAGAAAATAGTCCTCGAAACCTCGAATTTAACGGGAGTTCTTGATGCAAACTCTCTCTGCATGGTGAAGTCTGTTGGTGTTGTGAGCCTGAAGAAGAACAACATAACTGGTCTGCTCTCAGAAGACATTGGCAATTGCAGAGATTTAACTCACTTGTACATAAGTGGGAACGACTTTTCAGGTGAGCTTCCGGAGTCTCTCTCGCATTTGAATAATCTGAAAAGGGTTGATATCTCCAACAACAACTTCAATGGTGTGCTTCCTGATTTCGCAAAGATTTCAGGGTTGATATCTTTTCTTGCTCAGAACAACCGGCTTGATGGGCAAATCCCGGATTTTGATTTCTTCAACCTCAAGGAATTCAATGTCTCCAACAACAACTTCAGTGGCTCTATCCCTGATGTCAATGGCCTATTCGGAAAAGATAGCTTTTCGGGTAATCCTAAGCTGTGTGGAAAGCCACTGCAAAATGCCTGCCCTCCTGCAATACCAACTTCCGTAATGCCAAAGAAATCGAAAAAGTCCTCATCCGAAAAGCTGTTACTCTTCTCAATCAGAGAAGACAACGAAAAGGGCGGAAATAAAAAGATCGCGGCAAATGATGCTGCTAGCACTGTGCCTAGCACTACTATTTCTTCTGGTGAGTTGATGGCTGGTGGTGGTCATCATAAGATGGCAGAGTACTCGTTATCATCTGTTGAAAGCGGAATGGCTCCACCACTCGTAGTTCTCACAAATCCATTGCTGAGGGGGTTGAGTTTCGAGGATTTGCTCCGAGCTCCTGCTATATTGCTTGTCAGAGGGAAGAATGGAAGCCTCTACAAAATCATGCTTGATCGAGGGGTTAACTTGGTTGTGAAGAGGATCAGGAACAGTGGGATTTCGAGGGAAAAATTCAAGACTCGGTTGACACAAATCGATCAGGTTAAGTGTCGAAATGTCTTGCCAGCTGTTGCATTCTACTGTTCCAGACAAGAGAAGCTCTTGGTTTATGAGTATCAGCCTAATGGCAGTCTCTTTAACCTTCTTCATGAAATCATAGCTGAGTCATTGGCATTCATGCACCAAGAGCTACATGAACATGGCATTGCTCATGGCAACCTAAAGTCCATGAACATTTTGTTCAACATGTCCATGGAGCCCTGCATCAGTGAATACGGAATAATGGAGATTGAAAATCAAGACCAGTCACACCTTTCGCCAAACAGCGGGATTGAAAGTTCGAAAGCTGGCCATGCAGGAAGCACGTTCAAGGGGGAAGCTGGTGCAGCAAAACGGCTTGGATTTGCCCAGGTGGGTACACACTCGGTAGTTAGTGAGGAGTGGACTGTCGAAGTTTTTGACGAGGCTTTGATCCAAGAAGGTGCAAGTGAAGAAAGGATGGTGAAATTGTTGCAGGTAGCAATGCAGTGCATAAATGAGAGGCCAAGTATGAGTCGAGTTTCGGCGATGATTAAGTCGATCAAAGAGGAGGAGGAGAGATCTGTGTCATCTGATCCATAA

Protein Analysis

579

Amino Acids

63.44

Weight (kDa)

6.02

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 345 - 474 8.5e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 345 - 475 6.2e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05050
malus_domestica MD00G1171500.v1.1 MD06G1177700.v1.1 MD14G1184300.v1.1
prunus_persica Prupe.5G176900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0193991
rosa_laevigata RLG00000004225
rosa_roxburghii Rroxscaffold_3G00261640
rosa_rugosa Rorug07G0013000
rosa_samantha Rh7AG140200 Rh7BG140700 Rh7CG144200 Rh7DG141500
rosa_wichuraiana Rw7G011960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1621
AccII CGCG 1 cut(s) 827
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 5 cut(s) 62, 810, 827, 939, 1443
AclWI GGATC 6 cut(s) 12, 176, 189, 1106, 1582, 1724
AcoI YGGCCR 2 cut(s) 132, 1465
AcsI RAATTY 4 cut(s) 226, 259, 593, 1127
AcuI CTGAAG 2 cut(s) 344, 601
AfaI GTAC 3 cut(s) 392, 917, 1534
AfiI CCNNNNNNNGG 1 cut(s) 1442
AflIII ACRYGT 2 cut(s) 212, 1362
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 102, 1524
AjuI GAANNNNNNNTTGG 8 cut(s) 49, 81, 148, 180, 464, 496, 599, 631
AloI GAACNNNNNNTCC 2 cut(s) 1438, 1470
Alw21I GWGCWC 2 cut(s) 179, 1015
Alw26I GTCTC 4 cut(s) 144, 435, 607, 1253
AlwI GGATC 6 cut(s) 12, 176, 189, 1106, 1582, 1724
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 4 cut(s) 132, 643, 1465, 1658
ApeKI GCWGC 2 cut(s) 839, 1502
ApoI RAATTY 4 cut(s) 226, 259, 593, 1127
Asp700I GAANNNNTTC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 5 cut(s) 120, 310, 428, 887, 1630
AsuII TTCGAA 1 cut(s) 1457
AsuNHI GCTAGC 1 cut(s) 842
BaeGI GKGCMC 1 cut(s) 25
BalI TGGCCA 1 cut(s) 1467
BamHI GGATCC 1 cut(s) 181
BanII GRGCYC 3 cut(s) 179, 1015, 1377
BbsI GAAGAC 2 cut(s) 363, 801
Bbv12I GWGCWC 2 cut(s) 179, 1015
BbvCI CCTCAGC 1 cut(s) 980
BbvI GCAGC 2 cut(s) 826, 1514
BccI CCATC 4 cut(s) 548, 877, 901, 1609
BceAI ACGGC 1 cut(s) 1526
BcgI CGANNNNNNTGC 2 cut(s) 900, 934
BciT130I CCWGG 2 cut(s) 104, 1526
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 1 cut(s) 566
BcoDI GTCTC 4 cut(s) 144, 435, 607, 1253
BfaI CTAG 2 cut(s) 843, 855
BfuAI ACCTGC 1 cut(s) 1621
BglII AGATCT 1 cut(s) 1715
BisI GCNGC 3 cut(s) 828, 840, 1503
BlsI GCNGC 3 cut(s) 829, 841, 1504
BmcAI AGTACT 1 cut(s) 917
Bme1390I CCNGG 3 cut(s) 104, 566, 1526
BmgBI CACGTC 1 cut(s) 213
BmiI GGNNCC 3 cut(s) 183, 948, 1374
BmrFI CCNGG 3 cut(s) 104, 566, 1526
BmsI GCATC 3 cut(s) 268, 826, 1389
BmtI GCTAGC 1 cut(s) 846
BpiI GAAGAC 2 cut(s) 363, 801
BplI GAGNNNNNCTC 2 cut(s) 419, 451
Bpu10I CCTNAGC 2 cut(s) 678, 980
Bpu14I TTCGAA 1 cut(s) 1457
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 1 cut(s) 566
Bsa29I ATCGAT 1 cut(s) 1152
BsaJI CCNNGG 3 cut(s) 102, 1368, 1524
BsaWI WCCGGW 1 cut(s) 424
Bsc4I CCNNNNNNNGG 1 cut(s) 1442
Bse118I RCCGGY 2 cut(s) 130, 546
Bse1I ACTGG 2 cut(s) 346, 1420
Bse3DI GCAATG 3 cut(s) 974, 1320, 1644
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 2 cut(s) 104, 1526
BseCI ATCGAT 1 cut(s) 1152
BseDI CCNNGG 3 cut(s) 102, 1368, 1524
BseGI GGATG 2 cut(s) 761, 1620
BseLI CCNNNNNNNGG 1 cut(s) 1442
BseMI GCAATG 3 cut(s) 974, 1320, 1644
BseMII CTCAG 5 cut(s) 101, 366, 551, 971, 1269
BseNI ACTGG 2 cut(s) 346, 1420
BseRI GAGGAG 4 cut(s) 1568, 1718, 1721, 1724
BseSI GKGCMC 1 cut(s) 25
BseXI GCAGC 2 cut(s) 826, 1514
BsgI GTGCAG 1 cut(s) 1521
Bsh1236I CGCG 1 cut(s) 827
BshFI GGCC 4 cut(s) 134, 645, 1467, 1660
BshVI ATCGAT 1 cut(s) 1152
BsiHKAI GWGCWC 2 cut(s) 179, 1015
BsiSI CCGG 4 cut(s) 131, 425, 547, 566
BslI CCNNNNNNNGG 1 cut(s) 1442
BsmAI GTCTC 4 cut(s) 144, 435, 607, 1253
BsmBI CGTCTC 1 cut(s) 144
BsmI GAATGC 2 cut(s) 1190, 1289
BsnI GGCC 4 cut(s) 134, 645, 1467, 1660
Bsp119I TTCGAA 1 cut(s) 1457
Bsp1286I GDGCHC 4 cut(s) 25, 179, 1015, 1377
Bsp13I TCCGGA 1 cut(s) 424
Bsp1407I TGTACA 1 cut(s) 390
Bsp19I CCATGG 1 cut(s) 1368
BspACI CCGC 5 cut(s) 62, 810, 827, 939, 1443
BspANI GGCC 4 cut(s) 134, 645, 1467, 1660
BspCNI CTCAG 5 cut(s) 100, 365, 550, 972, 1270
BspDI ATCGAT 1 cut(s) 1152
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 1 cut(s) 827
BspHI TCATGA 1 cut(s) 1264
BspLI GGNNCC 3 cut(s) 183, 948, 1374
BspMI ACCTGC 1 cut(s) 1621
BspOI GCTAGC 1 cut(s) 846
BspPI GGATC 6 cut(s) 12, 176, 189, 1106, 1582, 1724
BspT104I TTCGAA 1 cut(s) 1457
BsrDI GCAATG 3 cut(s) 974, 1320, 1644
BsrFI RCCGGY 2 cut(s) 130, 546
BsrGI TGTACA 1 cut(s) 390
BsrI ACTGG 2 cut(s) 346, 1420
BssAI RCCGGY 2 cut(s) 130, 546
BssECI CCNNGG 3 cut(s) 102, 1368, 1524
BssT1I CCWWGG 1 cut(s) 1368
Bst2UI CCWGG 2 cut(s) 104, 1526
Bst4CI ACNGT 4 cut(s) 850, 1109, 1199, 1564
Bst6I CTCTTC 2 cut(s) 785, 1088
BstAPI GCANNNNNTGC 1 cut(s) 836
BstAUI TGTACA 1 cut(s) 390
BstBI TTCGAA 1 cut(s) 1457
BstC8I GCNNGC 5 cut(s) 99, 709, 844, 1182, 1465
BstDEI CTNAG 6 cut(s) 87, 352, 537, 678, 980, 1278
BstDSI CCRYGG 1 cut(s) 1368
BstF5I GGATG 2 cut(s) 761, 1620
BstFNI CGCG 1 cut(s) 827
BstMAI GTCTC 4 cut(s) 144, 435, 607, 1253
BstMWI GCNNNNNNNGC 5 cut(s) 556, 836, 1303, 1469, 1502
BstNI CCWGG 2 cut(s) 104, 1526
BstNSI RCATGY 1 cut(s) 1366
BstSCI CCNGG 3 cut(s) 102, 564, 1524
BstSLI GKGCMC 1 cut(s) 25
BstUI CGCG 1 cut(s) 827
BstV1I GCAGC 2 cut(s) 826, 1514
BstV2I GAAGAC 2 cut(s) 363, 801
BstX2I RGATCY 2 cut(s) 181, 1715
BstYI RGATCY 2 cut(s) 181, 1715
Bsu15I ATCGAT 1 cut(s) 1152
BsuRI GGCC 4 cut(s) 134, 645, 1467, 1660
BsuTUI ATCGAT 1 cut(s) 1152
BtgI CCRYGG 1 cut(s) 1368
BtgZI GCGATG 1 cut(s) 1697
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 2 cut(s) 761, 1620
BtsI GCAGTG 2 cut(s) 695, 1649
BtsIMutI CAGTG 6 cut(s) 625, 695, 846, 1114, 1390, 1649
BveI ACCTGC 1 cut(s) 1621
Cac8I GCNNGC 5 cut(s) 99, 709, 844, 1182, 1465
CciI TCATGA 1 cut(s) 1264
Cfr10I RCCGGY 2 cut(s) 130, 546
ClaI ATCGAT 1 cut(s) 1152
Csp6I GTAC 3 cut(s) 391, 916, 1533
CspCI CAANNNNNGTGG 2 cut(s) 601, 636
CviQI GTAC 3 cut(s) 391, 916, 1533
DdeI CTNAG 6 cut(s) 87, 352, 537, 678, 980, 1278
EaeI YGGCCR 2 cut(s) 132, 1465
Eam1104I CTCTTC 2 cut(s) 785, 1088
EarI CTCTTC 2 cut(s) 785, 1088
EciI GGCGGA 2 cut(s) 77, 825
Ecl136II GAGCTC 2 cut(s) 177, 1013
Eco130I CCWWGG 1 cut(s) 1368
Eco24I GRGCYC 3 cut(s) 179, 1015, 1377
Eco32I GATATC 2 cut(s) 466, 525
Eco53kI GAGCTC 2 cut(s) 177, 1013
Eco57I CTGAAG 2 cut(s) 344, 601
EcoICRI GAGCTC 2 cut(s) 177, 1013
EcoRI GAATTC 2 cut(s) 226, 593
EcoRII CCWGG 2 cut(s) 102, 1524
EcoRV GATATC 2 cut(s) 466, 525
EcoT14I CCWWGG 1 cut(s) 1368
EcoT38I GRGCYC 3 cut(s) 179, 1015, 1377
ErhI CCWWGG 1 cut(s) 1368
Esp3I CGTCTC 1 cut(s) 144
FauI CCCGC 1 cut(s) 1436
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 3 cut(s) 828, 840, 1503
FokI GGATG 2 cut(s) 748, 1627
FriOI GRGCYC 3 cut(s) 179, 1015, 1377
Fsp4HI GCNGC 3 cut(s) 828, 840, 1503
FspBI CTAG 2 cut(s) 843, 855
GluI GCNGC 3 cut(s) 828, 840, 1503
HaeIII GGCC 4 cut(s) 134, 645, 1467, 1660
HapII CCGG 4 cut(s) 131, 425, 547, 566
HincII GTYRAC 2 cut(s) 1081, 1143
HindII GTYRAC 2 cut(s) 1081, 1143
HinfI GANTC 6 cut(s) 65, 85, 428, 1135, 1280, 1669
HpaI GTTAAC 1 cut(s) 1081
HpaII CCGG 4 cut(s) 131, 425, 547, 566
HphI GGTGA 5 cut(s) 120, 310, 428, 887, 1630
Hpy166II GTNNAC 5 cut(s) 84, 1081, 1143, 1535, 1560
Hpy8I GTNNAC 5 cut(s) 84, 1081, 1143, 1535, 1560
HpyAV CCTTC 3 cut(s) 197, 1268, 1589
HpyCH4III ACNGT 4 cut(s) 850, 1109, 1199, 1564
HpyCH4IV ACGT 3 cut(s) 143, 212, 1481
HpyF10VI GCNNNNNNNGC 5 cut(s) 556, 836, 1303, 1469, 1502
HpyF3I CTNAG 6 cut(s) 87, 352, 537, 678, 980, 1278
HpySE526I ACGT 3 cut(s) 143, 212, 1481
Kpn2I TCCGGA 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 36
KspAI GTTAAC 1 cut(s) 1081
LmnI GCTCC 4 cut(s) 952, 1011, 1018, 1372
Lsp1109I GCAGC 2 cut(s) 826, 1514
LweI GCATC 3 cut(s) 268, 826, 1389
MaeI CTAG 2 cut(s) 843, 855
MaeII ACGT 3 cut(s) 143, 212, 1481
MaeIII GTNAC 4 cut(s) 208, 215, 774, 1422
MfeI CAATTG 1 cut(s) 367
MflI RGATCY 2 cut(s) 181, 1715
MhlI GDGCHC 4 cut(s) 25, 179, 1015, 1377
MlsI TGGCCA 1 cut(s) 1467
MluCI AATT 7 cut(s) 157, 226, 259, 367, 593, 1127, 1622
MluNI TGGCCA 1 cut(s) 1467
MlyI GAGTC 5 cut(s) 79, 437, 1129, 1289, 1678
MmeI TCCRAC 3 cut(s) 146, 495, 630
Mox20I TGGCCA 1 cut(s) 1467
MroI TCCGGA 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 226
MscI TGGCCA 1 cut(s) 1467
MseI TTAA 6 cut(s) 263, 380, 1080, 1161, 1254, 1689
MslI CAYNNNNRTG 3 cut(s) 56, 1314, 1367
Msp20I TGGCCA 1 cut(s) 1467
MspA1I CMGCKG 2 cut(s) 1184, 1443
MspI CCGG 4 cut(s) 131, 425, 547, 566
MspR9I CCNGG 3 cut(s) 104, 566, 1526
MunI CAATTG 1 cut(s) 367
Mva1269I GAATGC 2 cut(s) 1190, 1289
MvaI CCWGG 2 cut(s) 104, 1526
MvnI CGCG 1 cut(s) 827
MwoI GCNNNNNNNGC 5 cut(s) 556, 836, 1303, 1469, 1502
NciI CCSGG 1 cut(s) 566
NcoI CCATGG 1 cut(s) 1368
NheI GCTAGC 1 cut(s) 842
NlaIV GGNNCC 3 cut(s) 183, 948, 1374
NmuCI GTSAC 3 cut(s) 208, 215, 1422
NspI RCATGY 1 cut(s) 1366
NspV TTCGAA 1 cut(s) 1457
PagI TCATGA 1 cut(s) 1264
PciI ACATGT 1 cut(s) 1362
PctI GAATGC 2 cut(s) 1190, 1289
PdmI GAANNNNTTC 1 cut(s) 226
PfeI GAWTC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 564
PkrI GCNGC 3 cut(s) 829, 841, 1504
PleI GAGTC 5 cut(s) 79, 436, 1129, 1288, 1677
PpsI GAGTC 5 cut(s) 79, 436, 1129, 1288, 1677
PscI ACATGT 1 cut(s) 1362
Psp124BI GAGCTC 2 cut(s) 179, 1015
Psp6I CCWGG 2 cut(s) 102, 1524
PspGI CCWGG 2 cut(s) 102, 1524
PspN4I GGNNCC 3 cut(s) 183, 948, 1374
PsuI RGATCY 2 cut(s) 181, 1715
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 3 cut(s) 392, 917, 1534
RsaNI GTAC 3 cut(s) 391, 916, 1533
RseI CAYNNNNRTG 3 cut(s) 56, 1314, 1367
SacI GAGCTC 2 cut(s) 179, 1015
SaqAI TTAA 6 cut(s) 263, 380, 1080, 1161, 1254, 1689
SatI GCNGC 3 cut(s) 828, 840, 1503
ScaI AGTACT 1 cut(s) 917
SchI GAGTC 5 cut(s) 79, 437, 1129, 1289, 1678
ScrFI CCNGG 3 cut(s) 104, 566, 1526
SduI GDGCHC 4 cut(s) 25, 179, 1015, 1377
SfaNI GCATC 3 cut(s) 268, 826, 1389
SfuI TTCGAA 1 cut(s) 1457
SmiMI CAYNNNNRTG 3 cut(s) 56, 1314, 1367
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 7 cut(s) 157, 226, 259, 367, 593, 1127, 1622
SsiI CCGC 5 cut(s) 62, 810, 827, 939, 1443
SspMI CTAG 2 cut(s) 843, 855
SstI GAGCTC 2 cut(s) 179, 1015
StyD4I CCNGG 3 cut(s) 102, 564, 1524
StyI CCWWGG 1 cut(s) 1368
TaaI ACNGT 4 cut(s) 850, 1109, 1199, 1564
TaiI ACGT 3 cut(s) 146, 215, 1484
TasI AATT 7 cut(s) 157, 226, 259, 367, 593, 1127, 1622
TatI WGTACW 2 cut(s) 390, 915
TauI GCSGC 1 cut(s) 830
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 6 cut(s) 263, 380, 1080, 1161, 1254, 1689
Tru9I TTAA 6 cut(s) 263, 380, 1080, 1161, 1254, 1689
TscAI CASTG 6 cut(s) 625, 702, 853, 1114, 1390, 1649
TseFI GTSAC 3 cut(s) 208, 215, 1422
TseI GCWGC 2 cut(s) 839, 1502
Tsp45I GTSAC 3 cut(s) 208, 215, 1422
TspDTI ATGAA 5 cut(s) 1253, 1281, 1282, 1326, 1361
TspGWI ACGGA 3 cut(s) 168, 721, 1407
TspRI CASTG 6 cut(s) 625, 702, 853, 1114, 1390, 1649
XapI RAATTY 4 cut(s) 226, 259, 593, 1127
XceI RCATGY 1 cut(s) 1366
XmnI GAANNNNTTC 1 cut(s) 226
XspI CTAG 2 cut(s) 843, 855
ZrmI AGTACT 1 cut(s) 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.