RLG00000004225
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016842
Physical Location & Seq
Forward (+)
57377918 .. 57380790
2873 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004225

Sequence Viewer

Length: 2079 bp
ATGAGCACACACACACACAGAGAATGGTTCAAAGTCTTTGTCTGTATCAGCCAATTGGCACCAAGTGATTTCAGTTGCTTTCTTTTTGCTTTGCTGTTCCAAGTTACAGTTGTTCAAACCCTCTTCATGAAAAGGAATGCAAACCACAGAGCCATTTTTCACATACACTCAACTGGTGCATCGAAAATGGGTCGTGTCCCAATTTGGGTTCTCCCCATTGTGATATTTCTTATCTTCCGTGTTGTAAATTCAGAAGAAAATGAGGTGAAGCAAGCGCTGGTGCAGTTCATGCAGAAACTTTCACCAGGAAATGCAACAAGAGATGGCCATTGGGGTTGGAACATGAGCACAGATCCTTGCACAAGCAACTGGACTGGCGTGGTATGTGACAGGAATGGTTTTGTGAGGAAAATAGTTCTCGAAAAGGCCAACCTCACCGGAATTCTTGATGCGAGTTCTCTTTGTGTAACAAAGGCTCTTATTGGTTTGAGTCTCGAAAACAACAACATCACTGGTCTGCTCTCAGAAGACGTTGCACTTTGCAAACATTTGACTCACTTGTATTTGAGTGGGAATCATTTCTCTGGGGCCCTTCCTGAGTCTCTCTCCCAGTTGAGTAATCTGAAACGGTTTGATATATCCAATAACAACTTCTATGGTGAGCTTCCTGATATACAAAAGATTTCGGGCTTGTTATCTTTTCTTGCTCAAAACAATCAACTCGAGGGTCAAATCCCGGAGTTTGATTTCTCTAATCTTAATGAGTTCAATGTCTCCAACAACTTGTTCAGCGGTCCTATTCCTGATGTCAAAGGCCATTTCACTGCAAGCAGCTTTTCAGGTAATCCTAACCTTTGTGGGGAACCACTCGCTGCCTGCCCACCAGCTCCAGCAGTACCTATAAACCCAATGAAGAAGTCAAACAAGCCCTCATCCAAAACGGTTCTTATCTTTTCAGGCTATGTAATACTTGGCCTAGTAGTGGTGTTGTTTTTGGTATATAAGTTTTTTGTCCGAGGAAAACCATGTGAAGATAAAAATCAAAAGCCTAAAAAGAAGGCTGTGCCATTTGAAACTACTAGCAATATACCTAGTACTCCTGCTTCTAGTGAGTACAAGGATGGTGGGGGGAATCAGACATCAGAGTTTTCCCTGACATCTGTGGAAAGTGGAATAACCCCGCCGCTTGTTGTTCTCACCAGTCCTTTGTTGAGGAGGTTGACTTTCGAGCAGTTGCTCCGAGCTCCAGCTGAACTGCTTGGGAGAGGAAAGCATGGAAGCCTCTACAAAGTCTTGCTTGATGGAGGGGTTAACTTGGTTGTGAAGAGGATCAAGAATTGTGGGATTTCGAGCGATGATTTCAAGACTAGGATGAAGAAAATTAACCAAGTGAAGTGTCAAAATCTGTTGCCAGCGGTTGCATTTTATTGTTCAAGGCAAGAGAAGCTCTTGGTTTATGAATATCAACCAAATGGAAATCTATTTAATCTTCTTCATGGATCTCCAAATAGCCAAATCTTTGACTGGGGAAGCAGATTGAATGTTGCCGATAGCATTGCCGAGGCATTAGCCTTCATGCACCAAGAGTTAAATGAAGACGGTATTGCTCATGGTAACCTTAAGTCCATGAACATTTTGTTCAACATGGCCATGGAACCTTGCATCAGTGAATATGGGGTGATGGAGGCTGAAAATCATGAACAGTCGGTCCTTTCTCCCAAGAATGAATTCCGAAATGGCAATGCTAATAAAGTTCATGCATATAGCACCTTCAATGGTGATGTTTATGCTTTTGGGGTGATTCTTCTTGAGCTTCTAACAGGGAAGCTGGTCCAGAAAAACGGGTTTGACTTGCCTGGCTGGGTGAACTCAGTGGTCAAAGAGGAGTGGACTGTTGAGGTTTTCGACAGGGCTTTGATCTCGGAAGGTGCAAGCGAAGAAAGGATGGTGAACTTGCTGCAGGTAGCATTGAAGTGCATAAATACTTCTCCAACTGATAGGCCTGCTATGAGTCAAGTTTCGTTGATGATCAAGTCGATAAAAGAGGAAGAGGAGAGCTCTATAACTTATGATCCATGA

Protein Analysis

693

Amino Acids

76.67

Weight (kDa)

6.96

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 418 - 673 3e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 418 - 674 4.4e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016842)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05050
malus_domestica MD00G1171500.v1.1 MD06G1177700.v1.1 MD14G1184300.v1.1
prunus_persica Prupe.5G176900_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0193991
rosa_laevigata RLG00000004225
rosa_roxburghii Rroxscaffold_3G00261640
rosa_rugosa Rorug07G0013000
rosa_samantha Rh7AG140200 Rh7BG140700 Rh7CG144200 Rh7DG141500
rosa_wichuraiana Rw7G011960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1951
AccB1I GGYRCC 1 cut(s) 58
AciI CCGC 4 cut(s) 792, 1181, 1184, 1415
AclWI GGATC 4 cut(s) 347, 1337, 1507, 2066
AcoI YGGCCR 2 cut(s) 325, 1647
AcsI RAATTY 3 cut(s) 247, 441, 1727
AdeI CACNNNGTG 1 cut(s) 65
AfaI GTAC 3 cut(s) 897, 1096, 1115
AfeI AGCGCT 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 205, 859, 982
AflII CTTAAG 1 cut(s) 1619
AjnI CCWGG 2 cut(s) 304, 1855
AjuI GAANNNNNNNTTGG 6 cut(s) 635, 667, 770, 802, 1712, 1744
AloI GAACNNNNNNTCC 2 cut(s) 1692, 1724
AluBI AGCT 9 cut(s) 664, 834, 887, 1244, 1250, 1447, 1813, 1828, 2058
AluI AGCT 9 cut(s) 664, 834, 887, 1244, 1250, 1447, 1813, 1828, 2058
Alw21I GWGCWC 4 cut(s) 8, 350, 1246, 2060
Alw26I GTCTC 3 cut(s) 497, 606, 778
AlwI GGATC 4 cut(s) 347, 1337, 1507, 2066
Ama87I CYCGRG 1 cut(s) 722
Aor51HI AGCGCT 1 cut(s) 276
AoxI GGCC 7 cut(s) 325, 426, 588, 814, 973, 1647, 2000
ApaI GGGCCC 1 cut(s) 592
ApeKI GCWGC 3 cut(s) 831, 872, 1957
ApoI RAATTY 3 cut(s) 247, 441, 1727
ArsI GACNNNNNNTTYG 2 cut(s) 24, 56
Asp700I GAANNNNTTC 2 cut(s) 578, 1727
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 5 cut(s) 588, 589, 794, 1708, 1831
AsuC2I CCSGG 1 cut(s) 737
AvaI CYCGRG 1 cut(s) 722
AvaII GGWCC 3 cut(s) 794, 1708, 1831
BaeGI GKGCMC 1 cut(s) 592
BalI TGGCCA 2 cut(s) 327, 1649
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 3 cut(s) 592, 1246, 2060
BarI GAAGNNNNNNTAC 2 cut(s) 1087, 1119
BbsI GAAGAC 2 cut(s) 534, 1602
Bbv12I GWGCWC 4 cut(s) 8, 350, 1246, 2060
BbvI GCAGC 3 cut(s) 843, 859, 1944
BccI CCATC 5 cut(s) 317, 1115, 1295, 1675, 1939
BcgI CGANNNNNNTGC 2 cut(s) 1538, 1572
BciT130I CCWGG 2 cut(s) 306, 1857
BclI TGATCA 1 cut(s) 2028
BcnI CCSGG 1 cut(s) 737
BcoDI GTCTC 3 cut(s) 497, 606, 778
BfaI CTAG 5 cut(s) 977, 1080, 1092, 1107, 1368
BfmI CTRYAG 1 cut(s) 1958
BfoI RGCGCY 1 cut(s) 278
BfrI CTTAAG 1 cut(s) 1619
BfuAI ACCTGC 1 cut(s) 1951
BisI GCNGC 4 cut(s) 832, 873, 1184, 1958
BlsI GCNGC 4 cut(s) 833, 874, 1185, 1959
BmcAI AGTACT 1 cut(s) 1096
Bme1390I CCNGG 3 cut(s) 306, 737, 1857
Bme18I GGWCC 3 cut(s) 794, 1708, 1831
BmeT110I CYCGRG 1 cut(s) 722
BmgT120I GGNCC 5 cut(s) 588, 589, 794, 1708, 1831
BmiI GGNNCC 5 cut(s) 60, 589, 590, 864, 1656
BmrFI CCNGG 3 cut(s) 306, 737, 1857
BmrI ACTGGG 2 cut(s) 604, 1534
BmsI GCATC 3 cut(s) 188, 439, 1671
BmuI ACTGGG 2 cut(s) 604, 1534
BpiI GAAGAC 2 cut(s) 534, 1602
BplI GAGNNNNNCTC 4 cut(s) 590, 622, 2042, 2074
BpmI CTGGAG 2 cut(s) 873, 1230
BpuEI CTTGAG 1 cut(s) 1829
BpuMI CCSGG 1 cut(s) 737
BsaBI GATNNNNATC 1 cut(s) 1038
BsaJI CCNNGG 3 cut(s) 1015, 1560, 1650
BsaWI WCCGGW 1 cut(s) 437
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 859, 982
Bse1I ACTGG 7 cut(s) 178, 374, 379, 517, 610, 1200, 1529
Bse3DI GCAATG 2 cut(s) 1554, 1747
Bse8I GATNNNNATC 1 cut(s) 1038
BseBI CCWGG 2 cut(s) 306, 1857
BseDI CCNNGG 3 cut(s) 1015, 1560, 1650
BseGI GGATG 4 cut(s) 932, 1126, 1377, 1950
BseJI GATNNNNATC 1 cut(s) 1038
BseLI CCNNNNNNNGG 3 cut(s) 205, 859, 982
BseMI GCAATG 2 cut(s) 1554, 1747
BseMII CTCAG 3 cut(s) 537, 588, 1884
BseNI ACTGG 7 cut(s) 178, 374, 379, 517, 610, 1200, 1529
BseRI GAGGAG 3 cut(s) 1228, 1898, 2066
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 3 cut(s) 843, 859, 1944
BseYI CCCAGC 1 cut(s) 1860
BsgI GTGCAG 1 cut(s) 302
BshFI GGCC 7 cut(s) 327, 428, 590, 816, 975, 1649, 2002
BshNI GGYRCC 1 cut(s) 58
BsiHKAI GWGCWC 4 cut(s) 8, 350, 1246, 2060
BsiHKCI CYCGRG 1 cut(s) 722
BsiSI CCGG 2 cut(s) 438, 737
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 3 cut(s) 205, 859, 982
BsmAI GTCTC 3 cut(s) 497, 606, 778
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 142
BsnI GGCC 7 cut(s) 327, 428, 590, 816, 975, 1649, 2002
BsoBI CYCGRG 1 cut(s) 722
Bsp120I GGGCCC 1 cut(s) 588
Bsp1286I GDGCHC 5 cut(s) 8, 350, 592, 1246, 2060
Bsp143I GATC 6 cut(s) 352, 1329, 1499, 1917, 2028, 2071
Bsp19I CCATGG 1 cut(s) 1650
BspACI CCGC 4 cut(s) 792, 1181, 1184, 1415
BspANI GGCC 7 cut(s) 327, 428, 590, 816, 975, 1649, 2002
BspCNI CTCAG 3 cut(s) 536, 589, 1883
BspHI TCATGA 2 cut(s) 126, 1696
BspLI GGNNCC 5 cut(s) 60, 589, 590, 864, 1656
BspMAI CTGCAG 1 cut(s) 1962
BspMI ACCTGC 1 cut(s) 1951
BspPI GGATC 4 cut(s) 347, 1337, 1507, 2066
BspT107I GGYRCC 1 cut(s) 58
BspTI CTTAAG 1 cut(s) 1619
BsrDI GCAATG 2 cut(s) 1554, 1747
BsrI ACTGG 7 cut(s) 178, 374, 379, 517, 610, 1200, 1529
BssECI CCNNGG 3 cut(s) 1015, 1560, 1650
BssMI GATC 6 cut(s) 352, 1329, 1499, 1917, 2028, 2071
BssT1I CCWWGG 1 cut(s) 1650
Bst2UI CCWGG 2 cut(s) 306, 1857
Bst4CI ACNGT 6 cut(s) 109, 630, 943, 1601, 1704, 1894
Bst6I CTCTTC 3 cut(s) 128, 1319, 2043
BstAFI CTTAAG 1 cut(s) 1619
BstAPI GCANNNNNTGC 1 cut(s) 289
BstC8I GCNNGC 6 cut(s) 273, 829, 877, 1413, 1933, 2004
BstDEI CTNAG 3 cut(s) 523, 597, 1870
BstDSI CCRYGG 1 cut(s) 1650
BstEII GGTNACC 1 cut(s) 1613
BstF5I GGATG 4 cut(s) 932, 1126, 1377, 1950
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 6 cut(s) 355, 1332, 1502, 1920, 2031, 2074
BstMAI GTCTC 3 cut(s) 497, 606, 778
BstMBI GATC 6 cut(s) 352, 1329, 1499, 1917, 2028, 2071
BstMWI GCNNNNNNNGC 2 cut(s) 289, 1444
BstNI CCWGG 2 cut(s) 306, 1857
BstPI GGTNACC 1 cut(s) 1613
BstSCI CCNGG 3 cut(s) 304, 735, 1855
BstSFI CTRYAG 1 cut(s) 1958
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 3 cut(s) 843, 859, 1944
BstV2I GAAGAC 2 cut(s) 534, 1602
BstX2I RGATCY 2 cut(s) 352, 1499
BstYI RGATCY 2 cut(s) 352, 1499
BsuRI GGCC 7 cut(s) 327, 428, 590, 816, 975, 1649, 2002
BtgI CCRYGG 1 cut(s) 1650
BtgZI GCGATG 1 cut(s) 1368
BtsCI GGATG 4 cut(s) 932, 1126, 1377, 1950
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 4 cut(s) 510, 822, 1672, 1878
BveI ACCTGC 1 cut(s) 1951
Cac8I GCNNGC 6 cut(s) 273, 829, 877, 1413, 1933, 2004
CciI TCATGA 2 cut(s) 126, 1696
CfoI GCGC 1 cut(s) 277
Cfr13I GGNCC 5 cut(s) 588, 589, 794, 1708, 1831
Csp6I GTAC 3 cut(s) 896, 1095, 1114
CspCI CAANNNNNGTGG 4 cut(s) 1105, 1140, 1321, 1356
CviQI GTAC 3 cut(s) 896, 1095, 1114
DdeI CTNAG 3 cut(s) 523, 597, 1870
DpnI GATC 6 cut(s) 354, 1331, 1501, 1919, 2030, 2073
DpnII GATC 6 cut(s) 352, 1329, 1499, 1917, 2028, 2071
DraIII CACNNNGTG 1 cut(s) 65
EaeI YGGCCR 2 cut(s) 325, 1647
Eam1104I CTCTTC 3 cut(s) 128, 1319, 2043
EarI CTCTTC 3 cut(s) 128, 1319, 2043
Ecl136II GAGCTC 2 cut(s) 1244, 2058
Eco130I CCWWGG 1 cut(s) 1650
Eco147I AGGCCT 1 cut(s) 2002
Eco24I GRGCYC 3 cut(s) 592, 1246, 2060
Eco47I GGWCC 3 cut(s) 794, 1708, 1831
Eco47III AGCGCT 1 cut(s) 276
Eco53kI GAGCTC 2 cut(s) 1244, 2058
Eco88I CYCGRG 1 cut(s) 722
Eco91I GGTNACC 1 cut(s) 1613
EcoICRI GAGCTC 2 cut(s) 1244, 2058
EcoO109I RGGNCCY 2 cut(s) 588, 589
EcoO65I GGTNACC 1 cut(s) 1613
EcoRI GAATTC 2 cut(s) 441, 1727
EcoRII CCWGG 2 cut(s) 304, 1855
EcoT14I CCWWGG 1 cut(s) 1650
EcoT22I ATGCAT 1 cut(s) 1762
EcoT38I GRGCYC 3 cut(s) 592, 1246, 2060
ErhI CCWWGG 1 cut(s) 1650
FalI AAGNNNNNCTT 2 cut(s) 1281, 1313
FaqI GGGAC 1 cut(s) 182
FauI CCCGC 1 cut(s) 1188
FbaI TGATCA 1 cut(s) 2028
Fnu4HI GCNGC 4 cut(s) 832, 873, 1184, 1958
FokI GGATG 4 cut(s) 919, 1133, 1384, 1957
FriOI GRGCYC 3 cut(s) 592, 1246, 2060
Fsp4HI GCNGC 4 cut(s) 832, 873, 1184, 1958
FspBI CTAG 5 cut(s) 977, 1080, 1092, 1107, 1368
GlaI GCGC 1 cut(s) 276
GluI GCNGC 4 cut(s) 832, 873, 1184, 1958
GsaI CCCAGC 1 cut(s) 1864
GsuI CTGGAG 2 cut(s) 873, 1230
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 7 cut(s) 327, 428, 590, 816, 975, 1649, 2002
HapII CCGG 2 cut(s) 438, 737
HhaI GCGC 1 cut(s) 277
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HincII GTYRAC 2 cut(s) 1221, 1312
HindII GTYRAC 2 cut(s) 1221, 1312
HinfI GANTC 7 cut(s) 490, 553, 574, 599, 1132, 1801, 2011
HpaI GTTAAC 1 cut(s) 1312
HpaII CCGG 2 cut(s) 438, 737
Hpy166II GTNNAC 5 cut(s) 1221, 1312, 1867, 1890, 1951
Hpy188I TCNGA 9 cut(s) 253, 526, 624, 1016, 1137, 1144, 1241, 1733, 1924
Hpy8I GTNNAC 5 cut(s) 1221, 1312, 1867, 1890, 1951
HpyAV CCTTC 5 cut(s) 602, 1051, 1582, 1780, 1919
HpyCH4III ACNGT 6 cut(s) 109, 630, 943, 1601, 1704, 1894
HpyCH4IV ACGT 1 cut(s) 531
HpyF10VI GCNNNNNNNGC 2 cut(s) 289, 1444
HpyF3I CTNAG 3 cut(s) 523, 597, 1870
HpySE526I ACGT 1 cut(s) 531
HspAI GCGC 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 2028
KspAI GTTAAC 1 cut(s) 1312
Kzo9I GATC 6 cut(s) 352, 1329, 1499, 1917, 2028, 2071
LmnI GCTCC 3 cut(s) 892, 1242, 1249
Lsp1109I GCAGC 3 cut(s) 843, 859, 1944
LweI GCATC 3 cut(s) 188, 439, 1671
MaeI CTAG 5 cut(s) 977, 1080, 1092, 1107, 1368
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 4 cut(s) 103, 386, 466, 1613
MalI GATC 6 cut(s) 354, 1331, 1501, 1919, 2030, 2073
MboI GATC 6 cut(s) 352, 1329, 1499, 1917, 2028, 2071
MfeI CAATTG 1 cut(s) 53
MflI RGATCY 2 cut(s) 352, 1499
MhlI GDGCHC 5 cut(s) 8, 350, 592, 1246, 2060
MlsI TGGCCA 2 cut(s) 327, 1649
MluCI AATT 7 cut(s) 53, 201, 247, 441, 1336, 1380, 1727
MluNI TGGCCA 2 cut(s) 327, 1649
MlyI GAGTC 4 cut(s) 499, 547, 608, 2020
MmeI TCCRAC 3 cut(s) 317, 801, 2015
Mox20I TGGCCA 2 cut(s) 327, 1649
Mph1103I ATGCAT 1 cut(s) 1762
MroXI GAANNNNTTC 2 cut(s) 578, 1727
MscI TGGCCA 2 cut(s) 327, 1649
MseI TTAA 6 cut(s) 759, 1311, 1383, 1485, 1589, 1620
MslI CAYNNNNRTG 2 cut(s) 1649, 1972
Msp20I TGGCCA 2 cut(s) 327, 1649
MspA1I CMGCKG 3 cut(s) 792, 1250, 1415
MspCI CTTAAG 1 cut(s) 1619
MspI CCGG 2 cut(s) 438, 737
MspR9I CCNGG 3 cut(s) 306, 737, 1857
MunI CAATTG 1 cut(s) 53
Mva1269I GAATGC 1 cut(s) 142
MvaI CCWGG 2 cut(s) 306, 1857
MwoI GCNNNNNNNGC 2 cut(s) 289, 1444
NciI CCSGG 1 cut(s) 737
NcoI CCATGG 1 cut(s) 1650
NdeII GATC 6 cut(s) 352, 1329, 1499, 1917, 2028, 2071
NlaIV GGNNCC 5 cut(s) 60, 589, 590, 864, 1656
NmeAIII GCCGAG 1 cut(s) 1585
NmuCI GTSAC 1 cut(s) 386
NsiI ATGCAT 1 cut(s) 1762
PaeR7I CTCGAG 1 cut(s) 722
PagI TCATGA 2 cut(s) 126, 1696
PceI AGGCCT 1 cut(s) 2002
PctI GAATGC 1 cut(s) 142
PdmI GAANNNNTTC 2 cut(s) 578, 1727
PfeI GAWTC 3 cut(s) 574, 1132, 1801
PfoI TCCNGGA 1 cut(s) 735
PkrI GCNGC 4 cut(s) 833, 874, 1185, 1959
PleI GAGTC 4 cut(s) 498, 547, 607, 2019
PpsI GAGTC 4 cut(s) 498, 547, 607, 2019
Psp124BI GAGCTC 2 cut(s) 1246, 2060
Psp6I CCWGG 2 cut(s) 304, 1855
PspEI GGTNACC 1 cut(s) 1613
PspFI CCCAGC 1 cut(s) 1860
PspGI CCWGG 2 cut(s) 304, 1855
PspN4I GGNNCC 5 cut(s) 60, 589, 590, 864, 1656
PspOMI GGGCCC 1 cut(s) 588
PspPI GGNCC 5 cut(s) 588, 589, 794, 1708, 1831
PspXI VCTCGAGB 1 cut(s) 722
PstI CTGCAG 1 cut(s) 1962
PsuI RGATCY 2 cut(s) 352, 1499
PvuII CAGCTG 1 cut(s) 1250
RsaI GTAC 3 cut(s) 897, 1096, 1115
RsaNI GTAC 3 cut(s) 896, 1095, 1114
RseI CAYNNNNRTG 2 cut(s) 1649, 1972
SacI GAGCTC 2 cut(s) 1246, 2060
SaqAI TTAA 6 cut(s) 759, 1311, 1383, 1485, 1589, 1620
SatI GCNGC 4 cut(s) 832, 873, 1184, 1958
Sau3AI GATC 6 cut(s) 352, 1329, 1499, 1917, 2028, 2071
Sau96I GGNCC 5 cut(s) 588, 589, 794, 1708, 1831
ScaI AGTACT 1 cut(s) 1096
SchI GAGTC 4 cut(s) 499, 547, 608, 2020
ScrFI CCNGG 3 cut(s) 306, 737, 1857
SduI GDGCHC 5 cut(s) 8, 350, 592, 1246, 2060
SfaNI GCATC 3 cut(s) 188, 439, 1671
SfcI CTRYAG 1 cut(s) 1958
Sfr274I CTCGAG 1 cut(s) 722
SinI GGWCC 3 cut(s) 794, 1708, 1831
SlaI CTCGAG 1 cut(s) 722
SmiMI CAYNNNNRTG 2 cut(s) 1649, 1972
SmlI CTYRAG 3 cut(s) 722, 1619, 1808
SmoI CTYRAG 3 cut(s) 722, 1619, 1808
Sse9I AATT 7 cut(s) 53, 201, 247, 441, 1336, 1380, 1727
SseBI AGGCCT 1 cut(s) 2002
SsiI CCGC 4 cut(s) 792, 1181, 1184, 1415
SspMI CTAG 5 cut(s) 977, 1080, 1092, 1107, 1368
SstI GAGCTC 2 cut(s) 1246, 2060
StuI AGGCCT 1 cut(s) 2002
StyD4I CCNGG 3 cut(s) 304, 735, 1855
StyI CCWWGG 1 cut(s) 1650
TaaI ACNGT 6 cut(s) 109, 630, 943, 1601, 1704, 1894
TaiI ACGT 1 cut(s) 534
TaqI TCGA 8 cut(s) 182, 420, 495, 723, 1227, 1349, 1905, 2036
TaqII GACCGA 1 cut(s) 1696
TasI AATT 7 cut(s) 53, 201, 247, 441, 1336, 1380, 1727
TatI WGTACW 2 cut(s) 1094, 1113
TauI GCSGC 1 cut(s) 1186
TfiI GAWTC 3 cut(s) 574, 1132, 1801
Tru1I TTAA 6 cut(s) 759, 1311, 1383, 1485, 1589, 1620
Tru9I TTAA 6 cut(s) 759, 1311, 1383, 1485, 1589, 1620
TscAI CASTG 4 cut(s) 517, 829, 1672, 1878
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 3 cut(s) 831, 872, 1957
Tsp45I GTSAC 1 cut(s) 386
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 4 cut(s) 517, 829, 1672, 1878
Vha464I CTTAAG 1 cut(s) 1619
VpaK11BI GGWCC 3 cut(s) 794, 1708, 1831
XapI RAATTY 3 cut(s) 247, 441, 1727
XhoI CTCGAG 1 cut(s) 722
XmnI GAANNNNTTC 2 cut(s) 578, 1727
XspI CTAG 5 cut(s) 977, 1080, 1092, 1107, 1368
ZrmI AGTACT 1 cut(s) 1096
Zsp2I ATGCAT 1 cut(s) 1762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.