MD06G1184900.v1.1

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0024253
Physical Location & Seq
Forward (+)
32294215 .. 32297248
3034 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1184900.v1.1.491

Sequence Viewer

Length: 942 bp
ATGTCGGACACGTTTTGCCGCGACTGTAACAGTCTGACGGAGGTTGTCTTCGACCACAGAGCGGGCGACGCGATATGCTCCGAGTGCGGCCTCGTACTGGAGGCACACTCCGTCGACGAGACCGCCGAGTGGCGCTCCTTCGCCGACGACTCTAACGACAACGACCCGAACCGTGTTGGCGGACCCGTTAACCCGCTTTTGTCAGAAGCTGGGCTTTCTACCGTCATTGCCAGGCCCAAAAACGGCGGCGGCGGCGGCTCCTCTGAAGCCTTGCCATCCTCCGTCGGGAAGTGGCAGGGACGCGCGTCTAAGCCTGACCGTCATCTTCTCGACATCTTCGGTGCAATTGCCGTGATGGCCGACAGGTTGGGACTTGTTACAACCATAAAGGATCGAGCCAATGAAATATACAAGAAGGTAGAAGATCAAAAACCTCTCCGAGGGCGAAATCAAGATGCAATTGTGGCTGCTTGCCTCTACATTGCCTGTAGACAAGAAAATAAGCCTCGTACTGTGAAAGAAATATGCACTGCAGTCAGTGGAGCTACCAAGAAGGAAATTGGACGGGCAAAAGAGTTCATCTGGAAACACCTGGAGGCCGAGCTTGGTCCGGAAATGGGAACTATCCATGCTGGAGATTATTTGCGACGCTTCTGTTCCAATCTTGGAATGAAGAATCGAGATGTCAAGGCTGCCCAAGAAATGGTGCAGAACACTGAAGAGCTCGTTATAAGGAGGAGCCCTATATCGGTGGCAGCTGCCGTTATTTATACCATCAATCAACTAGCGGATGAGAAAAAGTCACTTAAAGATATCTCTGTTGCTACTCGAGTTGCTGAAGGGACGATCAAGAATTCCTACAGAGACCTGCATCCTCATCTGGCAAGAATAATACCGAACTGGTTTGCCAACGAGGAATGTGTCAAGAATCTGCAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

314

Amino Acids

34.38

Weight (kDa)

6.67

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_Ribbon_TF PF08271 5 - 47 2.1e-13 TFIIB zinc-binding
TFIIB PF00382 111 - 189 3.1e-20 Transcription factor TFIIB repeat
TFIIB PF00382 207 - 291 5.2e-12 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024253)

Species Orthologous Gene IDs
malus_domestica MD06G1184900.v1.1 MD14G1190800.v1.1
prunus_persica Prupe.5G183700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
Acc36I ACCTGC 1 cut(s) 876
AccB7I CCANNNNNTGG 1 cut(s) 703
AccBSI CCGCTC 1 cut(s) 62
AccI GTMKAC 2 cut(s) 114, 490
AccII CGCG 4 cut(s) 21, 71, 303, 305
AccIII TCCGGA 1 cut(s) 610
AclWI GGATC 1 cut(s) 399
AcoI YGGCCR 1 cut(s) 357
AcsI RAATTY 1 cut(s) 853
AcuI CTGAAG 3 cut(s) 285, 738, 858
AfaI GTAC 2 cut(s) 96, 511
AfiI CCNNNNNNNGG 9 cut(s) 61, 97, 129, 242, 285, 440, 617, 703, 748
AflIII ACRYGT 1 cut(s) 9
AjnI CCWGG 2 cut(s) 230, 591
AluBI AGCT 5 cut(s) 209, 545, 604, 724, 758
AluI AGCT 5 cut(s) 209, 545, 604, 724, 758
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 2 cut(s) 113, 858
AlwI GGATC 1 cut(s) 399
AlwNI CAGNNNCTG 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 828
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 4 cut(s) 88, 233, 357, 597
ApeKI GCWGC 5 cut(s) 467, 692, 755, 758, 934
ApoI RAATTY 1 cut(s) 853
AspLEI GCGC 2 cut(s) 135, 305
AspS9I GGNCC 3 cut(s) 182, 234, 608
AvaI CYCGRG 1 cut(s) 828
AvaII GGWCC 2 cut(s) 182, 608
BanII GRGCYC 2 cut(s) 726, 743
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 4 cut(s) 454, 679, 745, 767
BccI CCATC 3 cut(s) 283, 349, 782
BceAI ACGGC 3 cut(s) 259, 335, 746
BciT130I CCWGG 2 cut(s) 232, 593
BcoDI GTCTC 2 cut(s) 113, 858
BfaI CTAG 2 cut(s) 785, 940
BfmI CTRYAG 4 cut(s) 487, 531, 859, 932
BfoI RGCGCY 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 876
BglI GCCNNNNNGGC 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 232, 593
Bme18I GGWCC 2 cut(s) 182, 608
BmeT110I CYCGRG 1 cut(s) 828
BmgT120I GGNCC 3 cut(s) 182, 234, 608
BmiI GGNNCC 3 cut(s) 184, 259, 740
BmrFI CCNGG 2 cut(s) 232, 593
BmsI GCATC 2 cut(s) 445, 880
BoxI GACNNNNGTC 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 40
BplI GAGNNNNNCTC 4 cut(s) 92, 124, 119, 151
BpmI CTGGAG 3 cut(s) 119, 614, 654
BsaI GGTCTC 2 cut(s) 113, 858
BsaJI CCNNGG 1 cut(s) 439
BsaWI WCCGGW 1 cut(s) 610
Bsc4I CCNNNNNNNGG 9 cut(s) 61, 97, 129, 242, 285, 440, 617, 703, 748
Bse1I ACTGG 2 cut(s) 102, 905
Bse3DI GCAATG 2 cut(s) 225, 480
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 2 cut(s) 232, 593
BseDI CCNNGG 1 cut(s) 439
BseGI GGATG 3 cut(s) 275, 796, 871
BseLI CCNNNNNNNGG 9 cut(s) 61, 97, 129, 242, 285, 440, 617, 703, 748
BseMI GCAATG 2 cut(s) 225, 480
BseNI ACTGG 2 cut(s) 102, 905
BseRI GAGGAG 2 cut(s) 250, 751
BseXI GCAGC 4 cut(s) 454, 679, 745, 767
BseYI CCCAGC 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 4 cut(s) 21, 71, 303, 305
BshFI GGCC 4 cut(s) 90, 235, 359, 599
BsiHKAI GWGCWC 1 cut(s) 726
BsiHKCI CYCGRG 1 cut(s) 828
BsiSI CCGG 1 cut(s) 611
BslFI GGGAC 3 cut(s) 312, 384, 856
BslI CCNNNNNNNGG 9 cut(s) 61, 97, 129, 242, 285, 440, 617, 703, 748
BsmAI GTCTC 2 cut(s) 113, 858
BsmFI GGGAC 3 cut(s) 312, 384, 856
BsnI GGCC 4 cut(s) 90, 235, 359, 599
Bso31I GGTCTC 2 cut(s) 113, 858
BsoBI CYCGRG 1 cut(s) 828
Bsp1286I GDGCHC 2 cut(s) 726, 743
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 3 cut(s) 391, 424, 846
BspANI GGCC 4 cut(s) 90, 235, 359, 599
BspEI TCCGGA 1 cut(s) 610
BspFNI CGCG 4 cut(s) 21, 71, 303, 305
BspLI GGNNCC 3 cut(s) 184, 259, 740
BspMAI CTGCAG 2 cut(s) 535, 936
BspMI ACCTGC 1 cut(s) 876
BspPI GGATC 1 cut(s) 399
BspQI GCTCTTC 1 cut(s) 714
BspTNI GGTCTC 2 cut(s) 113, 858
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 2 cut(s) 225, 480
BsrI ACTGG 2 cut(s) 102, 905
BssECI CCNNGG 1 cut(s) 439
BssMI GATC 3 cut(s) 391, 424, 846
Bst2UI CCWGG 2 cut(s) 232, 593
Bst4CI ACNGT 6 cut(s) 26, 32, 173, 223, 320, 514
Bst6I CTCTTC 1 cut(s) 714
BstC8I GCNNGC 2 cut(s) 64, 472
BstDEI CTNAG 1 cut(s) 309
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 3 cut(s) 275, 796, 871
BstFNI CGCG 4 cut(s) 21, 71, 303, 305
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 2 cut(s) 135, 305
BstKTI GATC 3 cut(s) 394, 427, 849
BstMAI GTCTC 2 cut(s) 113, 858
BstMBI GATC 3 cut(s) 391, 424, 846
BstMWI GCNNNNNNNGC 6 cut(s) 68, 84, 252, 255, 356, 464
BstNI CCWGG 2 cut(s) 232, 593
BstPAI GACNNNNGTC 1 cut(s) 304
BstSCI CCNGG 2 cut(s) 230, 591
BstSFI CTRYAG 4 cut(s) 487, 531, 859, 932
BstUI CGCG 4 cut(s) 21, 71, 303, 305
BstV1I GCAGC 4 cut(s) 454, 679, 745, 767
BstV2I GAAGAC 1 cut(s) 40
BsuRI GGCC 4 cut(s) 90, 235, 359, 599
BtsCI GGATG 3 cut(s) 275, 796, 871
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 3 cut(s) 528, 544, 714
BveI ACCTGC 1 cut(s) 876
Cac8I GCNNGC 2 cut(s) 64, 472
CaiI CAGNNNCTG 1 cut(s) 209
CfoI GCGC 2 cut(s) 135, 305
Cfr13I GGNCC 3 cut(s) 182, 234, 608
CseI GACGC 4 cut(s) 77, 294, 309, 657
Csp6I GTAC 2 cut(s) 95, 510
CviAII CATG 1 cut(s) 629
CviQI GTAC 2 cut(s) 95, 510
DdeI CTNAG 1 cut(s) 309
DpnI GATC 3 cut(s) 393, 426, 848
DpnII GATC 3 cut(s) 391, 424, 846
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
EciI GGCGGA 1 cut(s) 195
Ecl136II GAGCTC 1 cut(s) 724
Eco24I GRGCYC 2 cut(s) 726, 743
Eco31I GGTCTC 2 cut(s) 113, 858
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 2 cut(s) 182, 608
Eco53kI GAGCTC 1 cut(s) 724
Eco57I CTGAAG 3 cut(s) 285, 738, 858
Eco88I CYCGRG 1 cut(s) 828
EcoICRI GAGCTC 1 cut(s) 724
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 2 cut(s) 230, 591
EcoRV GATATC 1 cut(s) 814
EcoT38I GRGCYC 2 cut(s) 726, 743
FaeI CATG 1 cut(s) 632
FaiI YATR 8 cut(s) 76, 386, 409, 526, 630, 731, 746, 771
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 3 cut(s) 312, 384, 856
FatI CATG 1 cut(s) 628
FauI CCCGC 2 cut(s) 55, 201
FblI GTMKAC 2 cut(s) 114, 490
FokI GGATG 3 cut(s) 262, 803, 858
FriOI GRGCYC 2 cut(s) 726, 743
FspBI CTAG 2 cut(s) 785, 940
GlaI GCGC 2 cut(s) 134, 304
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 3 cut(s) 119, 614, 654
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 4 cut(s) 90, 235, 359, 599
HapII CCGG 1 cut(s) 611
HgaI GACGC 4 cut(s) 77, 294, 309, 657
HhaI GCGC 2 cut(s) 135, 305
Hin1II CATG 1 cut(s) 632
Hin6I GCGC 2 cut(s) 133, 303
HinP1I GCGC 2 cut(s) 133, 303
HincII GTYRAC 2 cut(s) 115, 190
HindII GTYRAC 2 cut(s) 115, 190
HinfI GANTC 3 cut(s) 149, 676, 928
HpaI GTTAAC 1 cut(s) 190
HpaII CCGG 1 cut(s) 611
Hpy166II GTNNAC 3 cut(s) 115, 190, 491
Hpy188I TCNGA 6 cut(s) 7, 36, 82, 205, 265, 440
Hpy188III TCNNGA 8 cut(s) 286, 329, 452, 583, 611, 680, 850, 925
Hpy8I GTNNAC 3 cut(s) 115, 190, 491
Hpy99I CGWCG 6 cut(s) 71, 116, 119, 149, 287, 651
HpyAV CCTTC 4 cut(s) 148, 409, 547, 833
HpyCH4III ACNGT 6 cut(s) 26, 32, 173, 223, 320, 514
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 7 cut(s) 344, 458, 528, 533, 709, 871, 934
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 84, 252, 255, 356, 464
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 1 cut(s) 632
HspAI GCGC 2 cut(s) 133, 303
Kpn2I TCCGGA 1 cut(s) 610
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 3 cut(s) 391, 424, 846
LguI GCTCTTC 1 cut(s) 714
LmnI GCTCC 5 cut(s) 83, 140, 263, 542, 738
Lsp1109I GCAGC 4 cut(s) 454, 679, 745, 767
LweI GCATC 2 cut(s) 445, 880
MaeI CTAG 2 cut(s) 785, 940
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 3 cut(s) 26, 376, 801
MalI GATC 3 cut(s) 393, 426, 848
MbiI CCGCTC 1 cut(s) 62
MboI GATC 3 cut(s) 391, 424, 846
MboII GAAGA 6 cut(s) 40, 317, 328, 434, 685, 731
MfeI CAATTG 2 cut(s) 345, 459
MhlI GDGCHC 2 cut(s) 726, 743
MluCI AATT 4 cut(s) 345, 459, 558, 853
MlyI GAGTC 1 cut(s) 143
MroI TCCGGA 1 cut(s) 610
MseI TTAA 2 cut(s) 189, 807
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 1 cut(s) 611
MspR9I CCNGG 2 cut(s) 232, 593
MunI CAATTG 2 cut(s) 345, 459
MvaI CCWGG 2 cut(s) 232, 593
MvnI CGCG 4 cut(s) 21, 71, 303, 305
MwoI GCNNNNNNNGC 6 cut(s) 68, 84, 252, 255, 356, 464
NdeII GATC 3 cut(s) 391, 424, 846
NlaIII CATG 1 cut(s) 632
NlaIV GGNNCC 3 cut(s) 184, 259, 740
NmeAIII GCCGAG 2 cut(s) 151, 625
NmuCI GTSAC 1 cut(s) 801
PaeR7I CTCGAG 1 cut(s) 828
PciSI GCTCTTC 1 cut(s) 714
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 153
PfeI GAWTC 2 cut(s) 676, 928
PflMI CCANNNNNTGG 1 cut(s) 703
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PshAI GACNNNNGTC 1 cut(s) 304
PsiI TTATAA 1 cut(s) 731
Psp124BI GAGCTC 1 cut(s) 726
Psp6I CCWGG 2 cut(s) 230, 591
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 2 cut(s) 230, 591
PspN4I GGNNCC 3 cut(s) 184, 259, 740
PspPI GGNCC 3 cut(s) 182, 234, 608
PspXI VCTCGAGB 1 cut(s) 828
PstI CTGCAG 2 cut(s) 535, 936
PstNI CAGNNNCTG 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 2 cut(s) 96, 511
RsaNI GTAC 2 cut(s) 95, 510
SacI GAGCTC 1 cut(s) 726
SalI GTCGAC 1 cut(s) 113
SapI GCTCTTC 1 cut(s) 714
SaqAI TTAA 2 cut(s) 189, 807
Sau3AI GATC 3 cut(s) 391, 424, 846
Sau96I GGNCC 3 cut(s) 182, 234, 608
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 232, 593
SduI GDGCHC 2 cut(s) 726, 743
SfaNI GCATC 2 cut(s) 445, 880
SfcI CTRYAG 4 cut(s) 487, 531, 859, 932
Sfr274I CTCGAG 1 cut(s) 828
SgrDI CGTCGACG 1 cut(s) 113
SinI GGWCC 2 cut(s) 182, 608
SlaI CTCGAG 1 cut(s) 828
SmlI CTYRAG 1 cut(s) 828
SmoI CTYRAG 1 cut(s) 828
Sse9I AATT 4 cut(s) 345, 459, 558, 853
SspMI CTAG 2 cut(s) 785, 940
SstI GAGCTC 1 cut(s) 726
StyD4I CCNGG 2 cut(s) 230, 591
TaaI ACNGT 6 cut(s) 26, 32, 173, 223, 320, 514
TaiI ACGT 1 cut(s) 14
TaqI TCGA 6 cut(s) 51, 114, 330, 394, 679, 829
TasI AATT 4 cut(s) 345, 459, 558, 853
TauI GCSGC 6 cut(s) 21, 90, 249, 252, 255, 258
TfiI GAWTC 2 cut(s) 676, 928
Tru1I TTAA 2 cut(s) 189, 807
Tru9I TTAA 2 cut(s) 189, 807
TscAI CASTG 3 cut(s) 535, 544, 721
TseFI GTSAC 1 cut(s) 801
TseI GCWGC 5 cut(s) 467, 692, 755, 758, 934
Tsp45I GTSAC 1 cut(s) 801
TspDTI ATGAA 3 cut(s) 417, 568, 686
TspGWI ACGGA 3 cut(s) 53, 100, 271
TspRI CASTG 3 cut(s) 535, 544, 721
Van91I CCANNNNNTGG 1 cut(s) 703
VpaK11BI GGWCC 2 cut(s) 182, 608
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 1 cut(s) 853
XhoI CTCGAG 1 cut(s) 828
XmiI GTMKAC 2 cut(s) 114, 490
XspI CTAG 2 cut(s) 785, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.