Prupe.5G183700_v2.0.a1

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0024253
Physical Location & Seq
Forward (+)
15282535 .. 15285281
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G183700.1

Sequence Viewer

Length: 933 bp
ATGTCGGACATATTTTGCCGCGACTGTAACATGCTGACGGAGGTTGTGTTCGACCACTCAGCGGGCGACACGATATGCTCGGAGTGCGGGCTCGTGCTGGAGTCTCGCTCCGTCGACGAGACTTCCGAGTGGCGCTCCTTCGCCGACGAGTCCAACGGCAATGACCCGAACCGGGTCGGGGGACCTGTTAACCCGCTTTTGGCCGACGGTGGGCTTTCCACCGTTATCTCAGGTCCCAGTAACGGAGCTTCCTCCAATGCCTTGCCGTCCTCCGTCGGCAGGTGGCAGTGCCGCGCCTCTAAACCCGACCGGCATCTTCTCAACATCTTCAGTGCTATTGCTATTATGGCCGACAGGTTGGGGCTTGTCACAACCATAAAGGATCGAGCCAATGAGATATATAAGAAGGTGGAAGATCAAAGACCTCTGAGAGGACGCAATCAAGATGCAGTTGTGGCTGCTTGCCTCTACATTGCCTGCAGACAAGAAAATAAGCCTCGTACTGTGAAAGAAATATGCTCCGCAGTCAGTGGAGCTAGCAAGAAAGAAATTGGGCGGGCCAAAGAGTTCATTTGGAAACACCTTGAGGTTGAGATAGGTCAGGAAATAGGAACCATTCATGCTGGAGACTATCTGAGACGTTTTTGCTCCAATCTTGGAATGAAGAATCTGGAAGTCAAGGCTGCCCAAGAAACTGTGCAGAAATCTGAAGACCTTGTCATAAGGAGGAGCCCGATATCGGTGGCAGCTGCAGTTATATACATCATAACCCAACTATCGGATGACAAAAGGACTTTTAAAGATATTTCGCTCGCTACTAGAGTGGCTGAAGGGACCATCAAGAATTCCTACAAAGACTTGTATCCTCATCTGCCACGCCTAATACCGAACTGGTTTGCCAAGGAGGAAGATGTCAAGAATCTCTGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

311

Amino Acids

34.27

Weight (kDa)

6.95

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024253)

Species Orthologous Gene IDs
malus_domestica MD06G1184900.v1.1 MD14G1190800.v1.1
prunus_persica Prupe.5G183700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 270
Acc36I ACCTGC 1 cut(s) 270
AccI GTMKAC 1 cut(s) 114
AccII CGCG 2 cut(s) 21, 294
AciI CCGC 7 cut(s) 19, 62, 87, 194, 292, 522, 556
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 2 cut(s) 201, 348
AcsI RAATTY 1 cut(s) 844
AcuI CTGAAG 3 cut(s) 313, 729, 849
AfaI GTAC 1 cut(s) 502
AloI GAACNNNNNNTCC 2 cut(s) 32, 64
AluBI AGCT 3 cut(s) 248, 536, 749
AluI AGCT 3 cut(s) 248, 536, 749
Alw26I GTCTC 4 cut(s) 108, 113, 621, 631
AlwI GGATC 1 cut(s) 390
AoxI GGCC 3 cut(s) 201, 348, 558
ApeKI GCWGC 4 cut(s) 458, 683, 746, 749
ApoI RAATTY 1 cut(s) 844
AspLEI GCGC 2 cut(s) 135, 296
AspS9I GGNCC 4 cut(s) 182, 233, 558, 834
AsuC2I CCSGG 1 cut(s) 173
AsuNHI GCTAGC 1 cut(s) 536
AvaII GGWCC 3 cut(s) 182, 233, 834
BanII GRGCYC 2 cut(s) 93, 734
BarI GAAGNNNNNNTAC 2 cut(s) 232, 264
BauI CACGAG 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 717
BbvI GCAGC 4 cut(s) 445, 670, 736, 758
BccI CCATC 1 cut(s) 845
BceAI ACGGC 2 cut(s) 172, 250
BciVI GTATCC 1 cut(s) 873
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 4 cut(s) 108, 113, 621, 631
BfaI CTAG 2 cut(s) 537, 819
BfmI CTRYAG 2 cut(s) 478, 750
BfoI RGCGCY 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 270
BfuI GTATCC 1 cut(s) 873
BisI GCNGC 6 cut(s) 19, 292, 459, 684, 747, 750
BlsI GCNGC 6 cut(s) 20, 293, 460, 685, 748, 751
Bme1390I CCNGG 1 cut(s) 173
Bme18I GGWCC 3 cut(s) 182, 233, 834
BmgT120I GGNCC 4 cut(s) 182, 233, 558, 834
BmiI GGNNCC 5 cut(s) 183, 235, 613, 731, 835
BmrFI CCNGG 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 231
BmsI GCATC 2 cut(s) 322, 436
BmtI GCTAGC 1 cut(s) 540
BmuI ACTGGG 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 717
BplI GAGNNNNNCTC 4 cut(s) 92, 124, 119, 151
BpmI CTGGAG 2 cut(s) 119, 645
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 900
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bse118I RCCGGY 1 cut(s) 309
Bse1I ACTGG 2 cut(s) 237, 896
Bse3DI GCAATG 2 cut(s) 166, 471
BseDI CCNNGG 1 cut(s) 900
BseGI GGATG 1 cut(s) 787
BseMI GCAATG 2 cut(s) 166, 471
BseMII CTCAG 4 cut(s) 72, 243, 419, 626
BseNI ACTGG 2 cut(s) 237, 896
BseRI GAGGAG 1 cut(s) 742
BseXI GCAGC 4 cut(s) 445, 670, 736, 758
BsgI GTGCAG 1 cut(s) 719
Bsh1236I CGCG 2 cut(s) 21, 294
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 3 cut(s) 203, 350, 560
BsiEI CGRYCG 1 cut(s) 310
BsiSI CCGG 2 cut(s) 172, 310
BslFI GGGAC 3 cut(s) 195, 219, 847
BsmAI GTCTC 4 cut(s) 108, 113, 621, 631
BsmBI CGTCTC 1 cut(s) 631
BsmFI GGGAC 3 cut(s) 195, 219, 847
BsnI GGCC 3 cut(s) 203, 350, 560
Bsp1286I GDGCHC 2 cut(s) 93, 734
Bsp143I GATC 2 cut(s) 382, 415
BspACI CCGC 7 cut(s) 19, 62, 87, 194, 292, 522, 556
BspANI GGCC 3 cut(s) 203, 350, 560
BspCNI CTCAG 4 cut(s) 71, 242, 420, 627
BspFNI CGCG 2 cut(s) 21, 294
BspLI GGNNCC 5 cut(s) 183, 235, 613, 731, 835
BspMAI CTGCAG 2 cut(s) 482, 754
BspMI ACCTGC 1 cut(s) 270
BspOI GCTAGC 1 cut(s) 540
BspPI GGATC 1 cut(s) 390
BsrDI GCAATG 2 cut(s) 166, 471
BsrFI RCCGGY 1 cut(s) 309
BsrI ACTGG 2 cut(s) 237, 896
BssAI RCCGGY 1 cut(s) 309
BssECI CCNNGG 1 cut(s) 900
BssMI GATC 2 cut(s) 382, 415
BssSI CACGAG 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 900
Bst2BI CACGAG 1 cut(s) 92
Bst4CI ACNGT 5 cut(s) 26, 209, 223, 505, 697
BstC8I GCNNGC 7 cut(s) 64, 89, 463, 478, 538, 558, 813
BstDEI CTNAG 4 cut(s) 58, 229, 428, 635
BstENI CCTNNNNNAGG 1 cut(s) 429
BstF5I GGATG 1 cut(s) 787
BstFNI CGCG 2 cut(s) 21, 294
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 2 cut(s) 135, 296
BstKTI GATC 2 cut(s) 385, 418
BstMAI GTCTC 4 cut(s) 108, 113, 621, 631
BstMBI GATC 2 cut(s) 382, 415
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 84, 347, 455
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 171
BstSFI CTRYAG 2 cut(s) 478, 750
BstUI CGCG 2 cut(s) 21, 294
BstV1I GCAGC 4 cut(s) 445, 670, 736, 758
BstV2I GAAGAC 1 cut(s) 717
BsuI GTATCC 1 cut(s) 873
BsuRI GGCC 3 cut(s) 203, 350, 560
BtsCI GGATG 1 cut(s) 787
BtsI GCAGTG 1 cut(s) 293
BtsIMutI CAGTG 3 cut(s) 293, 337, 535
BveI ACCTGC 1 cut(s) 270
Cac8I GCNNGC 7 cut(s) 64, 89, 463, 478, 538, 558, 813
CfoI GCGC 2 cut(s) 135, 296
Cfr10I RCCGGY 1 cut(s) 309
Cfr13I GGNCC 4 cut(s) 182, 233, 558, 834
CseI GACGC 1 cut(s) 444
Csp6I GTAC 1 cut(s) 501
CviAII CATG 2 cut(s) 31, 620
CviQI GTAC 1 cut(s) 501
DdeI CTNAG 4 cut(s) 58, 229, 428, 635
DpnI GATC 2 cut(s) 384, 417
DpnII GATC 2 cut(s) 382, 415
DraI TTTAAA 1 cut(s) 799
EaeI YGGCCR 2 cut(s) 201, 348
Eco130I CCWWGG 1 cut(s) 900
Eco24I GRGCYC 2 cut(s) 93, 734
Eco32I GATATC 1 cut(s) 738
Eco47I GGWCC 3 cut(s) 182, 233, 834
Eco57I CTGAAG 3 cut(s) 313, 729, 849
EcoNI CCTNNNNNAGG 1 cut(s) 429
EcoO109I RGGNCCY 2 cut(s) 182, 233
EcoRI GAATTC 1 cut(s) 844
EcoRV GATATC 1 cut(s) 738
EcoT14I CCWWGG 1 cut(s) 900
EcoT38I GRGCYC 2 cut(s) 93, 734
ErhI CCWWGG 1 cut(s) 900
Esp3I CGTCTC 1 cut(s) 631
FaeI CATG 2 cut(s) 34, 623
FaqI GGGAC 3 cut(s) 195, 219, 847
FatI CATG 2 cut(s) 30, 619
FauI CCCGC 4 cut(s) 55, 80, 201, 549
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 6 cut(s) 19, 292, 459, 684, 747, 750
FokI GGATG 1 cut(s) 794
FriOI GRGCYC 2 cut(s) 93, 734
Fsp4HI GCNGC 6 cut(s) 19, 292, 459, 684, 747, 750
FspBI CTAG 2 cut(s) 537, 819
GlaI GCGC 2 cut(s) 134, 295
GluI GCNGC 6 cut(s) 19, 292, 459, 684, 747, 750
GsuI CTGGAG 2 cut(s) 119, 645
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 3 cut(s) 203, 350, 560
HapII CCGG 2 cut(s) 172, 310
HgaI GACGC 1 cut(s) 444
HhaI GCGC 2 cut(s) 135, 296
Hin1II CATG 2 cut(s) 34, 623
Hin6I GCGC 2 cut(s) 133, 294
HinP1I GCGC 2 cut(s) 133, 294
HincII GTYRAC 2 cut(s) 115, 190
HindII GTYRAC 2 cut(s) 115, 190
HinfI GANTC 4 cut(s) 101, 149, 667, 919
HpaI GTTAAC 1 cut(s) 190
HpaII CCGG 2 cut(s) 172, 310
Hpy166II GTNNAC 2 cut(s) 115, 190
Hpy188I TCNGA 7 cut(s) 7, 82, 127, 429, 636, 709, 781
Hpy188III TCNNGA 5 cut(s) 443, 602, 671, 841, 916
Hpy8I GTNNAC 2 cut(s) 115, 190
Hpy99I CGWCG 5 cut(s) 116, 119, 149, 209, 278
HpyAV CCTTC 3 cut(s) 148, 400, 824
HpyCH4III ACNGT 5 cut(s) 26, 209, 223, 505, 697
HpyCH4IV ACGT 1 cut(s) 640
HpyCH4V TGCA 5 cut(s) 449, 480, 700, 752, 927
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 347, 455
HpyF3I CTNAG 4 cut(s) 58, 229, 428, 635
HpySE526I ACGT 1 cut(s) 640
Hsp92II CATG 2 cut(s) 34, 623
HspAI GCGC 2 cut(s) 133, 294
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 2 cut(s) 382, 415
LmnI GCTCC 7 cut(s) 113, 140, 245, 524, 533, 653, 729
Lsp1109I GCAGC 4 cut(s) 445, 670, 736, 758
LweI GCATC 2 cut(s) 322, 436
MaeI CTAG 2 cut(s) 537, 819
MaeII ACGT 1 cut(s) 640
MaeIII GTNAC 3 cut(s) 26, 239, 367
MalI GATC 2 cut(s) 384, 417
MboI GATC 2 cut(s) 382, 415
MboII GAAGA 6 cut(s) 308, 319, 425, 676, 722, 920
MhlI GDGCHC 2 cut(s) 93, 734
MluCI AATT 2 cut(s) 549, 844
MlyI GAGTC 2 cut(s) 110, 158
MmeI TCCRAC 1 cut(s) 177
MseI TTAA 2 cut(s) 189, 798
MspA1I CMGCKG 2 cut(s) 62, 749
MspI CCGG 2 cut(s) 172, 310
MspR9I CCNGG 1 cut(s) 173
MvnI CGCG 2 cut(s) 21, 294
MwoI GCNNNNNNNGC 3 cut(s) 84, 347, 455
NciI CCSGG 1 cut(s) 173
NdeII GATC 2 cut(s) 382, 415
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 2 cut(s) 34, 623
NlaIV GGNNCC 5 cut(s) 183, 235, 613, 731, 835
NmuCI GTSAC 1 cut(s) 367
NspI RCATGY 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 2 cut(s) 667, 919
PflFI GACNNNGTC 1 cut(s) 716
PkrI GCNGC 6 cut(s) 20, 293, 460, 685, 748, 751
PleI GAGTC 2 cut(s) 109, 157
PpsI GAGTC 2 cut(s) 109, 157
PpuMI RGGWCCY 2 cut(s) 182, 233
Psp5II RGGWCCY 2 cut(s) 182, 233
PspN4I GGNNCC 5 cut(s) 183, 235, 613, 731, 835
PspPI GGNCC 4 cut(s) 182, 233, 558, 834
PspPPI RGGWCCY 2 cut(s) 182, 233
PstI CTGCAG 2 cut(s) 482, 754
PsyI GACNNNGTC 1 cut(s) 716
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 1 cut(s) 502
RsaNI GTAC 1 cut(s) 501
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 189, 798
SatI GCNGC 6 cut(s) 19, 292, 459, 684, 747, 750
Sau3AI GATC 2 cut(s) 382, 415
Sau96I GGNCC 4 cut(s) 182, 233, 558, 834
SchI GAGTC 2 cut(s) 110, 158
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 2 cut(s) 93, 734
SfaNI GCATC 2 cut(s) 322, 436
SfcI CTRYAG 2 cut(s) 478, 750
SgrDI CGTCGACG 1 cut(s) 113
SinI GGWCC 3 cut(s) 182, 233, 834
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 2 cut(s) 549, 844
SsiI CCGC 7 cut(s) 19, 62, 87, 194, 292, 522, 556
SspMI CTAG 2 cut(s) 537, 819
StyD4I CCNGG 1 cut(s) 171
StyI CCWWGG 1 cut(s) 900
TaaI ACNGT 5 cut(s) 26, 209, 223, 505, 697
TaiI ACGT 1 cut(s) 643
TaqI TCGA 3 cut(s) 51, 114, 385
TasI AATT 2 cut(s) 549, 844
TauI GCSGC 2 cut(s) 21, 294
TfiI GAWTC 2 cut(s) 667, 919
Tru1I TTAA 2 cut(s) 189, 798
Tru9I TTAA 2 cut(s) 189, 798
TscAI CASTG 3 cut(s) 293, 337, 535
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 4 cut(s) 458, 683, 746, 749
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 3 cut(s) 559, 608, 677
TspGWI ACGGA 4 cut(s) 53, 100, 258, 262
TspRI CASTG 3 cut(s) 293, 337, 535
Tth111I GACNNNGTC 1 cut(s) 716
VpaK11BI GGWCC 3 cut(s) 182, 233, 834
XagI CCTNNNNNAGG 1 cut(s) 429
XapI RAATTY 1 cut(s) 844
XceI RCATGY 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 2 cut(s) 537, 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.