MD14G1190800.v1.1

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0024253
Physical Location & Seq
Forward (+)
28184593 .. 28189482
4890 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1190800.v1.1.491

Sequence Viewer

Length: 939 bp
ATGTCGGACACGTTTTGCCGCGACTGTAATAGTCTGACGGAGGTTGTCTTCGACCACAGAGCGGGCTACGCGATATGCTCCGAGTGCGGCCTCGTACTGGAGGCGCATACCGTCGACGAGACCGCTGAGTGGCGCTCCTTCGCAGACGATTCCAACGACAAAGACCCGAACCGTGTCGGCGGACCCGTTAACCCGCTTTTGTCAGAAGCTGGGCTTTCCACCGTCATCGCCAGGCCCAGAAATGGCGGCTCCTCCTCCGAAGCCTTGCCCTCCTCCGTCGGGAAGTGGCAAGGCCGCGCCTCTAAGCCGGACCGTCATCTTCTCGACGTCTTCGGTGCAATTGCCGTGATGGCCGACAGGTTGGGACTTGTTACAACCATAAAGGATCGAGCCAATGAAATCTACAAGAAGGTAGAAGATCAAAAACCTCTCCGAGGGCGAAATCAAGATGCAGTTGTGGCTGCTTGCCTTTACATTGCTTGTAGACAAGAAAATAAGGCTCGTACTATGAAAGAAATATGCACCGCAGTCAGTGGAGCTACCAAGAAGGAAATTGGACGGGCAAAAGAGTTTATTTTGAAACACCTGGAGGCCGAGCTTGGTCAGGAAATGGGAACTATCCATGCTGGAGATTATCTGAGACGCTTTTGTTCCAATCTTGGAATGAAGAATCGAGATGTCAAGGCTGCCCAAGAAATGGTGCAGAACATTGAAGAGCTTGTTATAAGGTTGAGGAGCCCCATATCGGTGGCAGCTGCCGTTACTTATACCATTAATCAACTATCAGATGAGAAAATGTCTCTTAAAGAGATCTCGATTGCTACTCAAGTTGCCGAAGGGACGATCAAGAACTCCTACAAAGACCTGCATCCTCATCTAGCAAGGATAATACCGAACTGGTTCGCCAACGAGGACTGTGTCAAGAATCTGCAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

313

Amino Acids

34.43

Weight (kDa)

6.67

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_Ribbon_TF PF08271 5 - 47 5.9e-12 TFIIB zinc-binding
TFIIB PF00382 106 - 187 4e-20 Transcription factor TFIIB repeat
TFIIB PF00382 204 - 290 2.6e-11 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024253)

Species Orthologous Gene IDs
malus_domestica MD06G1184900.v1.1 MD14G1190800.v1.1
prunus_persica Prupe.5G183700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 725
AatII GACGTC 1 cut(s) 330
Acc36I ACCTGC 1 cut(s) 873
AccB7I CCANNNNNTGG 1 cut(s) 697
AccBSI CCGCTC 1 cut(s) 62
AccI GTMKAC 2 cut(s) 114, 484
AccII CGCG 3 cut(s) 21, 71, 297
AciI CCGC 9 cut(s) 19, 62, 87, 123, 180, 194, 246, 295, 525
AclWI GGATC 1 cut(s) 393
AcoI YGGCCR 1 cut(s) 351
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 2 cut(s) 96, 505
AfiI CCNNNNNNNGG 8 cut(s) 61, 97, 129, 242, 279, 434, 697, 745
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 2 cut(s) 580, 713
AjnI CCWGG 2 cut(s) 230, 585
AluBI AGCT 5 cut(s) 209, 539, 598, 718, 755
AluI AGCT 5 cut(s) 209, 539, 598, 718, 755
Alw26I GTCTC 3 cut(s) 113, 634, 804
AlwI GGATC 1 cut(s) 393
AlwNI CAGNNNCTG 1 cut(s) 209
AoxI GGCC 5 cut(s) 88, 233, 292, 351, 591
ApeKI GCWGC 5 cut(s) 461, 686, 752, 755, 931
AseI ATTAAT 1 cut(s) 774
Asp700I GAANNNNTTC 1 cut(s) 899
AspLEI GCGC 3 cut(s) 106, 135, 299
AspS9I GGNCC 3 cut(s) 182, 234, 310
AvaII GGWCC 2 cut(s) 182, 310
BanII GRGCYC 1 cut(s) 740
BbsI GAAGAC 2 cut(s) 40, 322
BbvI GCAGC 4 cut(s) 448, 673, 742, 764
BccI CCATC 1 cut(s) 343
BceAI ACGGC 2 cut(s) 329, 743
BciT130I CCWGG 2 cut(s) 232, 587
BcoDI GTCTC 3 cut(s) 113, 634, 804
BfaI CTAG 2 cut(s) 878, 937
BfmI CTRYAG 1 cut(s) 929
BfoI RGCGCY 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 873
BglI GCCNNNNNGGC 1 cut(s) 350
BglII AGATCT 1 cut(s) 810
BisI GCNGC 9 cut(s) 19, 88, 247, 295, 462, 687, 753, 756, 932
BlsI GCNGC 9 cut(s) 20, 89, 248, 296, 463, 688, 754, 757, 933
Bme1390I CCNGG 2 cut(s) 232, 587
Bme18I GGWCC 2 cut(s) 182, 310
BmgT120I GGNCC 3 cut(s) 182, 234, 310
BmiI GGNNCC 3 cut(s) 184, 250, 737
BmrFI CCNGG 2 cut(s) 232, 587
BmsI GCATC 2 cut(s) 439, 877
BpiI GAAGAC 2 cut(s) 40, 322
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 3 cut(s) 119, 608, 648
BpuEI CTTGAG 1 cut(s) 810
BsaHI GRCGYC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 433
Bsc4I CCNNNNNNNGG 8 cut(s) 61, 97, 129, 242, 279, 434, 697, 745
Bse1I ACTGG 2 cut(s) 102, 902
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 2 cut(s) 232, 587
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 1 cut(s) 868
BseLI CCNNNNNNNGG 8 cut(s) 61, 97, 129, 242, 279, 434, 697, 745
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 2 cut(s) 117, 629
BseNI ACTGG 2 cut(s) 102, 902
BseRI GAGGAG 4 cut(s) 241, 244, 262, 748
BseXI GCAGC 4 cut(s) 448, 673, 742, 764
BseYI CCCAGC 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 722
Bsh1236I CGCG 3 cut(s) 21, 71, 297
BshFI GGCC 5 cut(s) 90, 235, 294, 353, 593
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 2 cut(s) 378, 853
BslI CCNNNNNNNGG 8 cut(s) 61, 97, 129, 242, 279, 434, 697, 745
BsmAI GTCTC 3 cut(s) 113, 634, 804
BsmBI CGTCTC 1 cut(s) 634
BsmFI GGGAC 2 cut(s) 378, 853
BsnI GGCC 5 cut(s) 90, 235, 294, 353, 593
Bso31I GGTCTC 1 cut(s) 113
Bsp1286I GDGCHC 1 cut(s) 740
Bsp143I GATC 4 cut(s) 385, 418, 810, 843
BspACI CCGC 9 cut(s) 19, 62, 87, 123, 180, 194, 246, 295, 525
BspANI GGCC 5 cut(s) 90, 235, 294, 353, 593
BspCNI CTCAG 2 cut(s) 118, 630
BspFNI CGCG 3 cut(s) 21, 71, 297
BspLI GGNNCC 3 cut(s) 184, 250, 737
BspMAI CTGCAG 1 cut(s) 933
BspMI ACCTGC 1 cut(s) 873
BspPI GGATC 1 cut(s) 393
BspQI GCTCTTC 1 cut(s) 708
BspTNI GGTCTC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 2 cut(s) 102, 902
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 4 cut(s) 385, 418, 810, 843
BssNI GRCGYC 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 232, 587
Bst4CI ACNGT 6 cut(s) 26, 112, 173, 223, 314, 917
Bst6I CTCTTC 1 cut(s) 708
BstACI GRCGYC 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 64, 466
BstDEI CTNAG 3 cut(s) 126, 303, 638
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 868
BstFNI CGCG 3 cut(s) 21, 71, 297
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 3 cut(s) 106, 135, 299
BstKTI GATC 4 cut(s) 388, 421, 813, 846
BstMAI GTCTC 3 cut(s) 113, 634, 804
BstMBI GATC 4 cut(s) 385, 418, 810, 843
BstMWI GCNNNNNNNGC 4 cut(s) 68, 84, 350, 458
BstNI CCWGG 2 cut(s) 232, 587
BstSCI CCNGG 2 cut(s) 230, 585
BstSFI CTRYAG 1 cut(s) 929
BstUI CGCG 3 cut(s) 21, 71, 297
BstV1I GCAGC 4 cut(s) 448, 673, 742, 764
BstV2I GAAGAC 2 cut(s) 40, 322
BstX2I RGATCY 1 cut(s) 810
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 1 cut(s) 810
BsuRI GGCC 5 cut(s) 90, 235, 294, 353, 593
BtgZI GCGATG 1 cut(s) 211
BtsCI GGATG 1 cut(s) 868
BtsIMutI CAGTG 1 cut(s) 538
BveI ACCTGC 1 cut(s) 873
Cac8I GCNNGC 2 cut(s) 64, 466
CaiI CAGNNNCTG 1 cut(s) 209
CfoI GCGC 3 cut(s) 106, 135, 299
Cfr13I GGNCC 3 cut(s) 182, 234, 310
CpoI CGGWCCG 1 cut(s) 310
CseI GACGC 1 cut(s) 651
Csp6I GTAC 2 cut(s) 95, 504
CspI CGGWCCG 1 cut(s) 310
CviAII CATG 1 cut(s) 623
CviQI GTAC 2 cut(s) 95, 504
DdeI CTNAG 3 cut(s) 126, 303, 638
DpnI GATC 4 cut(s) 387, 420, 812, 845
DpnII GATC 4 cut(s) 385, 418, 810, 843
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
EciI GGCGGA 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 740
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 2 cut(s) 182, 310
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 230, 585
EcoT38I GRGCYC 1 cut(s) 740
Esp3I CGTCTC 1 cut(s) 634
FaeI CATG 1 cut(s) 626
FaiI YATR 9 cut(s) 76, 108, 380, 509, 520, 624, 725, 743, 768
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 2 cut(s) 378, 853
FatI CATG 1 cut(s) 622
FauI CCCGC 2 cut(s) 55, 201
FblI GTMKAC 2 cut(s) 114, 484
Fnu4HI GCNGC 9 cut(s) 19, 88, 247, 295, 462, 687, 753, 756, 932
FokI GGATG 1 cut(s) 855
FriOI GRGCYC 1 cut(s) 740
Fsp4HI GCNGC 9 cut(s) 19, 88, 247, 295, 462, 687, 753, 756, 932
FspBI CTAG 2 cut(s) 878, 937
GlaI GCGC 3 cut(s) 105, 134, 298
GluI GCNGC 9 cut(s) 19, 88, 247, 295, 462, 687, 753, 756, 932
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 3 cut(s) 119, 608, 648
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 5 cut(s) 90, 235, 294, 353, 593
HapII CCGG 1 cut(s) 308
HgaI GACGC 1 cut(s) 651
HhaI GCGC 3 cut(s) 106, 135, 299
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 1 cut(s) 626
Hin6I GCGC 3 cut(s) 104, 133, 297
HinP1I GCGC 3 cut(s) 104, 133, 297
HincII GTYRAC 2 cut(s) 115, 190
HindII GTYRAC 2 cut(s) 115, 190
HinfI GANTC 3 cut(s) 149, 670, 925
HpaI GTTAAC 1 cut(s) 190
HpaII CCGG 1 cut(s) 308
Hpy166II GTNNAC 3 cut(s) 115, 190, 485
Hpy188I TCNGA 8 cut(s) 7, 36, 82, 205, 259, 434, 639, 787
Hpy188III TCNNGA 8 cut(s) 280, 323, 446, 605, 674, 814, 847, 922
Hpy8I GTNNAC 3 cut(s) 115, 190, 485
Hpy99I CGWCG 4 cut(s) 116, 119, 281, 329
HpyAV CCTTC 4 cut(s) 148, 403, 541, 830
HpyCH4III ACNGT 6 cut(s) 26, 112, 173, 223, 314, 917
HpyCH4IV ACGT 2 cut(s) 11, 327
HpyCH4V TGCA 6 cut(s) 338, 452, 522, 703, 868, 931
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 84, 350, 458
HpyF3I CTNAG 3 cut(s) 126, 303, 638
HpySE526I ACGT 2 cut(s) 11, 327
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 1 cut(s) 626
HspAI GCGC 3 cut(s) 104, 133, 297
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 4 cut(s) 385, 418, 810, 843
LguI GCTCTTC 1 cut(s) 708
LmnI GCTCC 5 cut(s) 83, 140, 254, 536, 735
Lsp1109I GCAGC 4 cut(s) 448, 673, 742, 764
LweI GCATC 2 cut(s) 439, 877
MaeI CTAG 2 cut(s) 878, 937
MaeII ACGT 2 cut(s) 11, 327
MaeIII GTNAC 2 cut(s) 370, 760
MalI GATC 4 cut(s) 387, 420, 812, 845
MbiI CCGCTC 1 cut(s) 62
MboI GATC 4 cut(s) 385, 418, 810, 843
MboII GAAGA 6 cut(s) 40, 311, 322, 428, 679, 725
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 810
MhlI GDGCHC 1 cut(s) 740
MluCI AATT 2 cut(s) 339, 552
MmeI TCCRAC 1 cut(s) 177
MroXI GAANNNNTTC 1 cut(s) 899
MseI TTAA 3 cut(s) 189, 774, 804
MslI CAYNNNNRTG 1 cut(s) 746
MspA1I CMGCKG 2 cut(s) 125, 755
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 2 cut(s) 232, 587
MunI CAATTG 1 cut(s) 339
MvaI CCWGG 2 cut(s) 232, 587
MvnI CGCG 3 cut(s) 21, 71, 297
MwoI GCNNNNNNNGC 4 cut(s) 68, 84, 350, 458
NdeII GATC 4 cut(s) 385, 418, 810, 843
NlaIII CATG 1 cut(s) 626
NlaIV GGNNCC 3 cut(s) 184, 250, 737
NmeAIII GCCGAG 1 cut(s) 619
PciSI GCTCTTC 1 cut(s) 708
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 183
PdmI GAANNNNTTC 1 cut(s) 899
PfeI GAWTC 3 cut(s) 149, 670, 925
PflFI GACNNNGTC 1 cut(s) 917
PflMI CCANNNNNTGG 1 cut(s) 697
PkrI GCNGC 9 cut(s) 20, 89, 248, 296, 463, 688, 754, 757, 933
PshBI ATTAAT 1 cut(s) 774
PsiI TTATAA 1 cut(s) 725
Psp6I CCWGG 2 cut(s) 230, 585
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 2 cut(s) 230, 585
PspN4I GGNNCC 3 cut(s) 184, 250, 737
PspPI GGNCC 3 cut(s) 182, 234, 310
PstI CTGCAG 1 cut(s) 933
PstNI CAGNNNCTG 1 cut(s) 209
PsuI RGATCY 1 cut(s) 810
PsyI GACNNNGTC 1 cut(s) 917
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 2 cut(s) 96, 505
RsaNI GTAC 2 cut(s) 95, 504
RseI CAYNNNNRTG 1 cut(s) 746
Rsr2I CGGWCCG 1 cut(s) 310
RsrII CGGWCCG 1 cut(s) 310
SalI GTCGAC 1 cut(s) 113
SapI GCTCTTC 1 cut(s) 708
SaqAI TTAA 3 cut(s) 189, 774, 804
SatI GCNGC 9 cut(s) 19, 88, 247, 295, 462, 687, 753, 756, 932
Sau3AI GATC 4 cut(s) 385, 418, 810, 843
Sau96I GGNCC 3 cut(s) 182, 234, 310
ScrFI CCNGG 2 cut(s) 232, 587
SduI GDGCHC 1 cut(s) 740
SfaNI GCATC 2 cut(s) 439, 877
SfcI CTRYAG 1 cut(s) 929
SgrDI CGTCGACG 1 cut(s) 113
SinI GGWCC 2 cut(s) 182, 310
SmiMI CAYNNNNRTG 1 cut(s) 746
SmlI CTYRAG 1 cut(s) 825
SmoI CTYRAG 1 cut(s) 825
Sse9I AATT 2 cut(s) 339, 552
SsiI CCGC 9 cut(s) 19, 62, 87, 123, 180, 194, 246, 295, 525
SspMI CTAG 2 cut(s) 878, 937
StyD4I CCNGG 2 cut(s) 230, 585
TaaI ACNGT 6 cut(s) 26, 112, 173, 223, 314, 917
TaiI ACGT 2 cut(s) 14, 330
TaqI TCGA 6 cut(s) 51, 114, 324, 388, 673, 815
TasI AATT 2 cut(s) 339, 552
TauI GCSGC 4 cut(s) 21, 90, 249, 297
TfiI GAWTC 3 cut(s) 149, 670, 925
Tru1I TTAA 3 cut(s) 189, 774, 804
Tru9I TTAA 3 cut(s) 189, 774, 804
TscAI CASTG 1 cut(s) 538
TseI GCWGC 5 cut(s) 461, 686, 752, 755, 931
TspDTI ATGAA 3 cut(s) 411, 524, 680
TspGWI ACGGA 2 cut(s) 53, 265
TspRI CASTG 1 cut(s) 538
Tth111I GACNNNGTC 1 cut(s) 917
Van91I CCANNNNNTGG 1 cut(s) 697
VpaK11BI GGWCC 2 cut(s) 182, 310
VspI ATTAAT 1 cut(s) 774
XagI CCTNNNNNAGG 1 cut(s) 432
XmiI GTMKAC 2 cut(s) 114, 484
XmnI GAANNNNTTC 1 cut(s) 899
XspI CTAG 2 cut(s) 878, 937
ZraI GACGTC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.