MD06G1188000.v1.1

Domain of unknown function (DUF2828)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0022311
Physical Location & Seq
Reverse (-)
32527440 .. 32529344
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1188000.v1.1.491

Sequence Viewer

Length: 1905 bp
ATGAAAACTGGGCAAGTGCAGCTCCTTTCTGTTCTGAAAGCATGCCAAAGCTATGTTTGCAATCTCTCACAACTCACAAGATCGGCGGCTGCTCGGACGCCTCTCGTCGGTCCTCCGGAGCTCAAACTACCGGTAACATCACAACTGTCACTGCCACAACCCCAATTAGCACACCAACCCAGCCCAACCCCCTCCGCGAAGGAGGTCCCGTACTCCTTCGGTAACCCCTGCCACAATCTCTATTTCCACGTGGTAGGCAACGAACCTGAGAGCCATGAGCATCTGAACCAACTGCTTCCGGAGGCCTGGTCCCACAATCCCCTAACCACCCTTAAGCTCATCGGTAACCTGCTAGACAACCACCACCTAACTGGAAAAGGCTACGACGAATTGTTTTACACGGCAGCGTTTTGGCTCTACCAGAACCACCCCAAAACCCTAGCGCACAACGTGGTGCCGATCGTCGGGTCTTTGGGGGCTTTTGAAAACATTCTTGAGGTTCTTTACGGCATTGTAGATGTCAATGCCAAGAGCAAGAGGACATCGAGGGAACACGATTGCTATTTTGAGTACATAAACTTGATACAGGAGAAGGCAGGCGACACGGGGACTAAAAGATGTATCGCGATGGCTAGAAATGCTGTCGACATGTATGAGCGTGACCCCAATTATCAATTGTTACACGAGAGGGTTTCGGATGTTTACGCTGAGTGCTTGAAATGTGATATCCAAAATTTGAAGGAATATGAGAAGCAGAAGCTGGATAATGACAATGATCCTAAGGACTCCGAGGTGTTGGAGTTAACCTCTGCAGCGAAATGGTGCCCTTCTGTTGATTCTTTCTTTGATCGCGCGACTTTGATATGCGAGAGCATTGCAAAGAAGGTGTTCCCGCGAGAGGAATTCGTAGAGGGTGTTAGTGTTGAGGAGGAGGCAAATTATGTGTCCAGGGTCCGAGAGAGGCTGATGCGTGAGGTTTTGGAGCCTTTGAGGGCAGCCATAGCGAAAGGCTATAAAGATAATACTCGAAGATGGCAAAGGTTAACCCCGAAGAGCAAGTTTGTGCAGACGGACTTTCCAAATGATTTTAATCCAAGAGTGTCAAGGTCAGCAATTGTTAAGTATTTGGAGGATGTCAAAGCTGGAGGCAAGTCGAAGATTGAAGCAGGTGCCATGCTTCCAGATGAGATCATAAGCAATGTGCACGACCACAATTATGGGCAACTGGCTGAGCATCAGTGGAAGGCAATGGTGGAGGAGATCTTTTTAAAAGAGGGGAAGTGGAAAAGCTGCTTGGCTGTGTGTGACGTCTCCGGAAGGATGTGTGGTAACCCCATGAATGTGTCATTGGGTTTGGGACTGTTGGTGTCTGAACTGGGTGAAGAGCCATGGAAAGGAAAGGTCTTCACTTTTAGTCGAAACCCTCAACTACTTACCATACAAGGGGATGGTCTTAAGTCCAGGTGCACGTTCATGAGGATGATGGACAACCAGGACTGGGATGGCGAAACTGATTTTCAGAAGGTGTTTGATTTGATTCTGAACGTGGCTCTGAATGAAAATTTGAAGCCAGAGCAAATGATCAAGAGGGTGTATGTGTTCACCAGCGACCAAGATTTTGATGATGCTTCGGGCGGTAGCTGGAACACAGATTATGAGGCAATACAAAGAAAGTACAAGGAGAAAGGGTACGGAGATGTGGTGCCACGCATAGTGTTTTGGGATCTGAACGATGACAAGTACCCTATGGCACTTCCTGCAGAACAAGGGGTGGCCACATTGGCTGGCTACTCCAAGAAATTTTTCAAGTTCTTCTTGGACAATGATGGGGATATTGGGCCCGACCATGTCGTGGAAGCAGCCATCTCTGGCGAACGGTACCAAAATCTGGCTGTAGTGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2828

Protein Analysis

635

Amino Acids

71.84

Weight (kDa)

5.52

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2828 PF11443 72 - 157 7.8e-21 Domain of unknown function (DUF2828)
DUF2828 PF11443 194 - 421 4.4e-39 Domain of unknown function (DUF2828)
DUF7788 PF25043 430 - 612 4.2e-60 Domain of unknown function (DUF7788)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022311)

Species Orthologous Gene IDs
malus_domestica MD06G1187700.v1.1 MD06G1187900.v1.1 MD06G1188000.v1.1
prunus_persica Prupe.5G186100_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1157
AatII GACGTC 1 cut(s) 1311
Acc36I ACCTGC 2 cut(s) 357, 1157
Acc65I GGTACC 1 cut(s) 1878
AccB1I GGYRCC 5 cut(s) 454, 822, 1169, 1702, 1878
AccB7I CCANNNNNTGG 2 cut(s) 1852, 1888
AccI GTMKAC 1 cut(s) 645
AccII CGCG 5 cut(s) 197, 626, 852, 854, 895
AccIII TCCGGA 3 cut(s) 115, 298, 1313
AciI CCGC 4 cut(s) 86, 195, 893, 1635
AclWI GGATC 2 cut(s) 770, 1731
AcoI YGGCCR 1 cut(s) 1773
AcsI RAATTY 4 cut(s) 733, 902, 1561, 1799
AcvI CACGTG 1 cut(s) 250
AcyI GRCGYC 2 cut(s) 98, 1308
AdeI CACNNNGTG 1 cut(s) 451
AfaI GTAC 6 cut(s) 212, 572, 1676, 1691, 1742, 1880
AfiI CCNNNNNNNGG 8 cut(s) 107, 464, 898, 1394, 1443, 1498, 1852, 1888
AflII CTTAAG 2 cut(s) 332, 1454
AflIII ACRYGT 1 cut(s) 648
AgeI ACCGGT 1 cut(s) 130
AgsI TTSAA 6 cut(s) 485, 718, 739, 1163, 1567, 1807
AjnI CCWGG 4 cut(s) 305, 947, 1460, 1491
AjuI GAANNNNNNNTTGG 6 cut(s) 1277, 1309, 1331, 1363, 1787, 1819
AluBI AGCT 8 cut(s) 22, 51, 121, 337, 760, 1142, 1290, 1641
AluI AGCT 8 cut(s) 22, 51, 121, 337, 760, 1142, 1290, 1641
Alw21I GWGCWC 3 cut(s) 123, 1206, 1469
Alw26I GTCTC 1 cut(s) 1315
Alw44I GTGCAC 2 cut(s) 1202, 1465
AlwI GGATC 2 cut(s) 770, 1731
AlwNI CAGNNNCTG 1 cut(s) 760
Aor13HI TCCGGA 3 cut(s) 115, 298, 1313
AoxI GGCC 3 cut(s) 303, 1773, 1838
ApaI GGGCCC 1 cut(s) 1842
ApaLI GTGCAC 2 cut(s) 1202, 1465
ApeKI GCWGC 7 cut(s) 19, 89, 404, 812, 995, 1290, 1859
ApoI RAATTY 4 cut(s) 733, 902, 1561, 1799
ArsI GACNNNNNNTTYG 4 cut(s) 929, 961, 1601, 1633
AsiGI ACCGGT 1 cut(s) 130
Asp700I GAANNNNTTC 3 cut(s) 489, 887, 1802
Asp718I GGTACC 1 cut(s) 1878
AspLEI GCGC 2 cut(s) 445, 854
AspS9I GGNCC 6 cut(s) 110, 205, 309, 952, 1838, 1839
AsuHPI GGTGA 2 cut(s) 1391, 1594
AvaII GGWCC 4 cut(s) 110, 205, 309, 952
AxyI CCTNAGG 1 cut(s) 780
BaeGI GKGCMC 4 cut(s) 827, 1206, 1469, 1842
BalI TGGCCA 1 cut(s) 1775
BanI GGYRCC 5 cut(s) 454, 822, 1169, 1702, 1878
BanII GRGCYC 2 cut(s) 123, 1842
BauI CACGAG 1 cut(s) 683
BbrPI CACGTG 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 1396
Bbv12I GWGCWC 3 cut(s) 123, 1206, 1469
BbvI GCAGC 7 cut(s) 31, 76, 416, 824, 1007, 1277, 1871
BccI CCATC 7 cut(s) 622, 1026, 1442, 1477, 1496, 1820, 1871
BceAI ACGGC 2 cut(s) 417, 523
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BciT130I CCWGG 4 cut(s) 307, 949, 1462, 1493
BclI TGATCA 1 cut(s) 1581
BcoDI GTCTC 1 cut(s) 1315
BfaI CTAG 3 cut(s) 353, 440, 633
BfmI CTRYAG 3 cut(s) 810, 1758, 1893
BfrI CTTAAG 2 cut(s) 332, 1454
BfuAI ACCTGC 2 cut(s) 357, 1157
BglI GCCNNNNNGGC 1 cut(s) 1781
BglII AGATCT 1 cut(s) 1260
BisI GCNGC 8 cut(s) 20, 87, 90, 405, 813, 996, 1291, 1860
BlpI GCTNAGC 1 cut(s) 1230
BlsI GCNGC 8 cut(s) 21, 88, 91, 406, 814, 997, 1292, 1861
Bme1390I CCNGG 4 cut(s) 307, 949, 1462, 1493
Bme18I GGWCC 4 cut(s) 110, 205, 309, 952
BmgT120I GGNCC 6 cut(s) 110, 205, 309, 952, 1838, 1839
BmrFI CCNGG 4 cut(s) 307, 949, 1462, 1493
BmrI ACTGGG 3 cut(s) 18, 1385, 1507
BmsI GCATC 4 cut(s) 289, 957, 1243, 1615
BmuI ACTGGG 3 cut(s) 18, 1385, 1507
BpiI GAAGAC 1 cut(s) 1396
BplI GAGNNNNNCTC 2 cut(s) 791, 823
BpmI CTGGAG 1 cut(s) 1164
Bpu1102I GCTNAGC 1 cut(s) 1230
BpuEI CTTGAG 1 cut(s) 515
BsaAI YACGTR 1 cut(s) 250
BsaBI GATNNNNATC 1 cut(s) 1089
BsaHI GRCGYC 2 cut(s) 98, 1308
BsaJI CCNNGG 3 cut(s) 789, 948, 1388
BsaWI WCCGGW 4 cut(s) 115, 130, 298, 1313
BsaXI ACNNNNNCTCC 8 cut(s) 194, 224, 293, 323, 1673, 1686, 1703, 1716
Bsc4I CCNNNNNNNGG 8 cut(s) 107, 464, 898, 1394, 1443, 1498, 1852, 1888
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 5 cut(s) 13, 376, 1230, 1380, 1502
Bse21I CCTNAGG 1 cut(s) 780
Bse3DI GCAATG 3 cut(s) 873, 1204, 1254
Bse8I GATNNNNATC 1 cut(s) 1089
BseAI TCCGGA 3 cut(s) 115, 298, 1313
BseBI CCWGG 4 cut(s) 307, 949, 1462, 1493
BseDI CCNNGG 3 cut(s) 789, 948, 1388
BseGI GGATG 6 cut(s) 703, 1138, 1326, 1453, 1485, 1507
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 8 cut(s) 107, 464, 898, 1394, 1443, 1498, 1852, 1888
BseMI GCAATG 3 cut(s) 873, 1204, 1254
BseMII CTCAG 3 cut(s) 258, 699, 1221
BseNI ACTGG 5 cut(s) 13, 376, 1230, 1380, 1502
BseRI GAGGAG 3 cut(s) 941, 944, 1271
BseSI GKGCMC 4 cut(s) 827, 1206, 1469, 1842
BseXI GCAGC 7 cut(s) 31, 76, 416, 824, 1007, 1277, 1871
BseYI CCCAGC 1 cut(s) 179
BsgI GTGCAG 2 cut(s) 38, 1085
Bsh1236I CGCG 5 cut(s) 197, 626, 852, 854, 895
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 3 cut(s) 305, 1775, 1840
BshNI GGYRCC 5 cut(s) 454, 822, 1169, 1702, 1878
BshTI ACCGGT 1 cut(s) 130
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 3 cut(s) 123, 1206, 1469
BsiSI CCGG 4 cut(s) 116, 131, 299, 1314
BslFI GGGAC 4 cut(s) 191, 295, 622, 1371
BslI CCNNNNNNNGG 8 cut(s) 107, 464, 898, 1394, 1443, 1498, 1852, 1888
BsmAI GTCTC 1 cut(s) 1315
BsmBI CGTCTC 1 cut(s) 1315
BsmFI GGGAC 4 cut(s) 191, 295, 622, 1371
BsnI GGCC 3 cut(s) 305, 1775, 1840
Bsp120I GGGCCC 1 cut(s) 1838
Bsp1286I GDGCHC 5 cut(s) 123, 827, 1206, 1469, 1842
Bsp13I TCCGGA 3 cut(s) 115, 298, 1313
Bsp143I GATC 8 cut(s) 80, 459, 775, 847, 1188, 1260, 1581, 1723
Bsp1720I GCTNAGC 1 cut(s) 1230
Bsp19I CCATGG 1 cut(s) 1388
Bsp68I TCGCGA 1 cut(s) 626
BspACI CCGC 4 cut(s) 86, 195, 893, 1635
BspANI GGCC 3 cut(s) 305, 1775, 1840
BspCNI CTCAG 3 cut(s) 259, 700, 1222
BspEI TCCGGA 3 cut(s) 115, 298, 1313
BspFNI CGCG 5 cut(s) 197, 626, 852, 854, 895
BspHI TCATGA 1 cut(s) 1473
BspMAI CTGCAG 2 cut(s) 814, 1762
BspMI ACCTGC 2 cut(s) 357, 1157
BspPI GGATC 2 cut(s) 770, 1731
BspQI GCTCTTC 2 cut(s) 1046, 1377
BspT107I GGYRCC 5 cut(s) 454, 822, 1169, 1702, 1878
BspTI CTTAAG 2 cut(s) 332, 1454
BsrDI GCAATG 3 cut(s) 873, 1204, 1254
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 5 cut(s) 13, 376, 1230, 1380, 1502
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 789, 948, 1388
BssMI GATC 8 cut(s) 80, 459, 775, 847, 1188, 1260, 1581, 1723
BssNI GRCGYC 2 cut(s) 98, 1308
BssSI CACGAG 1 cut(s) 683
BssT1I CCWWGG 1 cut(s) 1388
Bst2BI CACGAG 1 cut(s) 683
Bst2UI CCWGG 4 cut(s) 307, 949, 1462, 1493
Bst4CI ACNGT 3 cut(s) 147, 1362, 1878
Bst6I CTCTTC 2 cut(s) 1046, 1377
BstACI GRCGYC 2 cut(s) 98, 1308
BstAFI CTTAAG 2 cut(s) 332, 1454
BstAPI GCANNNNNTGC 1 cut(s) 1757
BstBAI YACGTR 1 cut(s) 250
BstC8I GCNNGC 3 cut(s) 43, 598, 1786
BstDEI CTNAG 4 cut(s) 267, 708, 780, 1230
BstDSI CCRYGG 1 cut(s) 1388
BstEII GGTNACC 3 cut(s) 221, 344, 1328
BstF5I GGATG 6 cut(s) 703, 1138, 1326, 1453, 1485, 1507
BstFNI CGCG 5 cut(s) 197, 626, 852, 854, 895
BstHHI GCGC 2 cut(s) 445, 854
BstKTI GATC 8 cut(s) 83, 462, 778, 850, 1191, 1263, 1584, 1726
BstMAI GTCTC 1 cut(s) 1315
BstMBI GATC 8 cut(s) 80, 459, 775, 847, 1188, 1260, 1581, 1723
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 6 cut(s) 19, 57, 638, 1001, 1757, 1781
BstNI CCWGG 4 cut(s) 307, 949, 1462, 1493
BstNSI RCATGY 2 cut(s) 45, 652
BstPI GGTNACC 3 cut(s) 221, 344, 1328
BstSCI CCNGG 4 cut(s) 305, 947, 1460, 1491
BstSFI CTRYAG 3 cut(s) 810, 1758, 1893
BstSLI GKGCMC 4 cut(s) 827, 1206, 1469, 1842
BstUI CGCG 5 cut(s) 197, 626, 852, 854, 895
BstV1I GCAGC 7 cut(s) 31, 76, 416, 824, 1007, 1277, 1871
BstV2I GAAGAC 1 cut(s) 1396
BstX2I RGATCY 2 cut(s) 1260, 1723
BstXI CCANNNNNNTGG 2 cut(s) 371, 1217
BstYI RGATCY 2 cut(s) 1260, 1723
Bsu36I CCTNAGG 1 cut(s) 780
BsuRI GGCC 3 cut(s) 305, 1775, 1840
BtgI CCRYGG 1 cut(s) 1388
BtgZI GCGATG 1 cut(s) 641
BtsCI GGATG 6 cut(s) 703, 1138, 1326, 1453, 1485, 1507
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 2 cut(s) 149, 1244
BtuMI TCGCGA 1 cut(s) 626
BveI ACCTGC 2 cut(s) 357, 1157
Cac8I GCNNGC 3 cut(s) 43, 598, 1786
CaiI CAGNNNCTG 1 cut(s) 760
CciI TCATGA 1 cut(s) 1473
CfoI GCGC 2 cut(s) 445, 854
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 6 cut(s) 110, 205, 309, 952, 1838, 1839
CseI GACGC 1 cut(s) 106
Csp6I GTAC 6 cut(s) 211, 571, 1675, 1690, 1741, 1879
CspAI ACCGGT 1 cut(s) 130
CviAII CATG 8 cut(s) 42, 275, 649, 1174, 1336, 1389, 1474, 1847
CviQI GTAC 6 cut(s) 211, 571, 1675, 1690, 1741, 1879
DdeI CTNAG 4 cut(s) 267, 708, 780, 1230
DpnI GATC 8 cut(s) 82, 461, 777, 849, 1190, 1262, 1583, 1725
DpnII GATC 8 cut(s) 80, 459, 775, 847, 1188, 1260, 1581, 1723
DraI TTTAAA 1 cut(s) 1269
DraIII CACNNNGTG 1 cut(s) 451
EaeI YGGCCR 1 cut(s) 1773
Eam1104I CTCTTC 2 cut(s) 1046, 1377
EarI CTCTTC 2 cut(s) 1046, 1377
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 1388
Eco147I AGGCCT 1 cut(s) 305
Eco24I GRGCYC 2 cut(s) 123, 1842
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 4 cut(s) 110, 205, 309, 952
Eco53kI GAGCTC 1 cut(s) 121
Eco72I CACGTG 1 cut(s) 250
Eco81I CCTNAGG 1 cut(s) 780
Eco91I GGTNACC 3 cut(s) 221, 344, 1328
EcoICRI GAGCTC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 205
EcoO65I GGTNACC 3 cut(s) 221, 344, 1328
EcoRI GAATTC 1 cut(s) 902
EcoRII CCWGG 4 cut(s) 305, 947, 1460, 1491
EcoRV GATATC 1 cut(s) 727
EcoT14I CCWWGG 1 cut(s) 1388
EcoT38I GRGCYC 2 cut(s) 123, 1842
ErhI CCWWGG 1 cut(s) 1388
Esp3I CGTCTC 1 cut(s) 1315
FaeI CATG 8 cut(s) 45, 278, 652, 1177, 1339, 1392, 1477, 1850
FalI AAGNNNNNCTT 2 cut(s) 1799, 1831
FaqI GGGAC 4 cut(s) 191, 295, 622, 1371
FatI CATG 8 cut(s) 41, 274, 648, 1173, 1335, 1388, 1473, 1846
FauI CCCGC 1 cut(s) 900
FbaI TGATCA 1 cut(s) 1581
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 8 cut(s) 20, 87, 90, 405, 813, 996, 1291, 1860
FokI GGATG 6 cut(s) 710, 1145, 1333, 1460, 1492, 1514
FriOI GRGCYC 2 cut(s) 123, 1842
Fsp4HI GCNGC 8 cut(s) 20, 87, 90, 405, 813, 996, 1291, 1860
FspBI CTAG 3 cut(s) 353, 440, 633
GlaI GCGC 2 cut(s) 444, 853
GluI GCNGC 8 cut(s) 20, 87, 90, 405, 813, 996, 1291, 1860
GsaI CCCAGC 1 cut(s) 183
GsuI CTGGAG 1 cut(s) 1164
HaeIII GGCC 3 cut(s) 305, 1775, 1840
HapII CCGG 4 cut(s) 116, 131, 299, 1314
HgaI GACGC 1 cut(s) 106
HhaI GCGC 2 cut(s) 445, 854
Hin1I GRCGYC 2 cut(s) 98, 1308
Hin1II CATG 8 cut(s) 45, 278, 652, 1177, 1339, 1392, 1477, 1850
Hin6I GCGC 2 cut(s) 443, 852
HinP1I GCGC 2 cut(s) 443, 852
HincII GTYRAC 3 cut(s) 646, 804, 1044
HindII GTYRAC 3 cut(s) 646, 804, 1044
HinfI GANTC 3 cut(s) 785, 836, 1537
HpaI GTTAAC 2 cut(s) 804, 1044
HpaII CCGG 4 cut(s) 116, 131, 299, 1314
HphI GGTGA 2 cut(s) 1391, 1594
Hpy166II GTNNAC 8 cut(s) 646, 703, 804, 1044, 1204, 1467, 1602, 1900
Hpy188III TCNNGA 8 cut(s) 116, 299, 494, 625, 1181, 1314, 1474, 1585
Hpy8I GTNNAC 8 cut(s) 646, 703, 804, 1044, 1204, 1467, 1602, 1900
Hpy99I CGWCG 3 cut(s) 110, 389, 467
HpyAV CCTTC 9 cut(s) 193, 226, 586, 733, 837, 877, 1237, 1311, 1516
HpyCH4III ACNGT 3 cut(s) 147, 1362, 1878
HpyCH4IV ACGT 5 cut(s) 249, 450, 1308, 1469, 1545
HpyCH4V TGCA 8 cut(s) 19, 60, 812, 878, 1066, 1204, 1467, 1760
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 57, 638, 1001, 1757, 1781
HpyF3I CTNAG 4 cut(s) 267, 708, 780, 1230
HpySE526I ACGT 5 cut(s) 249, 450, 1308, 1469, 1545
Hsp92I GRCGYC 2 cut(s) 98, 1308
Hsp92II CATG 8 cut(s) 45, 278, 652, 1177, 1339, 1392, 1477, 1850
HspAI GCGC 2 cut(s) 443, 852
Kpn2I TCCGGA 3 cut(s) 115, 298, 1313
KpnI GGTACC 1 cut(s) 1882
Ksp22I TGATCA 1 cut(s) 1581
KspAI GTTAAC 2 cut(s) 804, 1044
Kzo9I GATC 8 cut(s) 80, 459, 775, 847, 1188, 1260, 1581, 1723
LguI GCTCTTC 2 cut(s) 1046, 1377
LmnI GCTCC 3 cut(s) 27, 118, 982
Lsp1109I GCAGC 7 cut(s) 31, 76, 416, 824, 1007, 1277, 1871
LweI GCATC 4 cut(s) 289, 957, 1243, 1615
MaeI CTAG 3 cut(s) 353, 440, 633
MaeII ACGT 5 cut(s) 249, 450, 1308, 1469, 1545
MaeIII GTNAC 8 cut(s) 133, 147, 221, 344, 659, 678, 1304, 1328
MalI GATC 8 cut(s) 82, 461, 777, 849, 1190, 1262, 1583, 1725
MboI GATC 8 cut(s) 80, 459, 775, 847, 1188, 1260, 1581, 1723
MboII GAAGA 6 cut(s) 1041, 1063, 1168, 1394, 1396, 1804
MfeI CAATTG 2 cut(s) 674, 1113
MflI RGATCY 2 cut(s) 1260, 1723
MhlI GDGCHC 5 cut(s) 123, 827, 1206, 1469, 1842
MlsI TGGCCA 1 cut(s) 1775
MluNI TGGCCA 1 cut(s) 1775
MlyI GAGTC 1 cut(s) 779
MmeI TCCRAC 1 cut(s) 777
Mox20I TGGCCA 1 cut(s) 1775
MroI TCCGGA 3 cut(s) 115, 298, 1313
MroXI GAANNNNTTC 3 cut(s) 489, 887, 1802
MscI TGGCCA 1 cut(s) 1775
MseI TTAA 7 cut(s) 333, 803, 1043, 1089, 1119, 1268, 1455
MslI CAYNNNNRTG 4 cut(s) 1215, 1340, 1472, 1478
Msp20I TGGCCA 1 cut(s) 1775
MspCI CTTAAG 2 cut(s) 332, 1454
MspI CCGG 4 cut(s) 116, 131, 299, 1314
MspR9I CCNGG 4 cut(s) 307, 949, 1462, 1493
MunI CAATTG 2 cut(s) 674, 1113
MvaI CCWGG 4 cut(s) 307, 949, 1462, 1493
MvnI CGCG 5 cut(s) 197, 626, 852, 854, 895
MwoI GCNNNNNNNGC 6 cut(s) 19, 57, 638, 1001, 1757, 1781
NcoI CCATGG 1 cut(s) 1388
NdeII GATC 8 cut(s) 80, 459, 775, 847, 1188, 1260, 1581, 1723
NlaIII CATG 8 cut(s) 45, 278, 652, 1177, 1339, 1392, 1477, 1850
NmuCI GTSAC 3 cut(s) 147, 659, 1304
NruI TCGCGA 1 cut(s) 626
NspI RCATGY 2 cut(s) 45, 652
PaeI GCATGC 1 cut(s) 45
PagI TCATGA 1 cut(s) 1473
PaqCI CACCTGC 1 cut(s) 1157
PceI AGGCCT 1 cut(s) 305
PciI ACATGT 1 cut(s) 648
PciSI GCTCTTC 2 cut(s) 1046, 1377
PdmI GAANNNNTTC 3 cut(s) 489, 887, 1802
PfeI GAWTC 2 cut(s) 836, 1537
PflFI GACNNNGTC 1 cut(s) 1847
PflMI CCANNNNNTGG 2 cut(s) 1852, 1888
PinAI ACCGGT 1 cut(s) 130
PkrI GCNGC 8 cut(s) 21, 88, 91, 406, 814, 997, 1292, 1861
Ple19I CGATCG 1 cut(s) 462
PleI GAGTC 1 cut(s) 779
PmaCI CACGTG 1 cut(s) 250
PmlI CACGTG 1 cut(s) 250
PpsI GAGTC 1 cut(s) 779
Ppu21I YACGTR 1 cut(s) 250
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 4 cut(s) 305, 947, 1460, 1491
PspCI CACGTG 1 cut(s) 250
PspEI GGTNACC 3 cut(s) 221, 344, 1328
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 4 cut(s) 305, 947, 1460, 1491
PspOMI GGGCCC 1 cut(s) 1838
PspPI GGNCC 6 cut(s) 110, 205, 309, 952, 1838, 1839
PspPPI RGGWCCY 1 cut(s) 205
PstI CTGCAG 2 cut(s) 814, 1762
PstNI CAGNNNCTG 1 cut(s) 760
PsuI RGATCY 2 cut(s) 1260, 1723
PsyI GACNNNGTC 1 cut(s) 1847
PvuI CGATCG 1 cut(s) 462
RruI TCGCGA 1 cut(s) 626
RsaI GTAC 6 cut(s) 212, 572, 1676, 1691, 1742, 1880
RsaNI GTAC 6 cut(s) 211, 571, 1675, 1690, 1741, 1879
RseI CAYNNNNRTG 4 cut(s) 1215, 1340, 1472, 1478
SacI GAGCTC 1 cut(s) 123
SalI GTCGAC 1 cut(s) 644
SapI GCTCTTC 2 cut(s) 1046, 1377
SaqAI TTAA 7 cut(s) 333, 803, 1043, 1089, 1119, 1268, 1455
SatI GCNGC 8 cut(s) 20, 87, 90, 405, 813, 996, 1291, 1860
Sau3AI GATC 8 cut(s) 80, 459, 775, 847, 1188, 1260, 1581, 1723
Sau96I GGNCC 6 cut(s) 110, 205, 309, 952, 1838, 1839
SchI GAGTC 1 cut(s) 779
ScrFI CCNGG 4 cut(s) 307, 949, 1462, 1493
SduI GDGCHC 5 cut(s) 123, 827, 1206, 1469, 1842
SfaNI GCATC 4 cut(s) 289, 957, 1243, 1615
SfcI CTRYAG 3 cut(s) 810, 1758, 1893
SinI GGWCC 4 cut(s) 110, 205, 309, 952
SmiMI CAYNNNNRTG 4 cut(s) 1215, 1340, 1472, 1478
SmlI CTYRAG 3 cut(s) 332, 494, 1454
SmoI CTYRAG 3 cut(s) 332, 494, 1454
SphI GCATGC 1 cut(s) 45
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 4 cut(s) 86, 195, 893, 1635
SspMI CTAG 3 cut(s) 353, 440, 633
SstI GAGCTC 1 cut(s) 123
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 4 cut(s) 305, 947, 1460, 1491
StyI CCWWGG 1 cut(s) 1388
TaaI ACNGT 3 cut(s) 147, 1362, 1878
TaiI ACGT 5 cut(s) 252, 453, 1311, 1472, 1548
TaqI TCGA 5 cut(s) 545, 645, 1027, 1154, 1417
TaqII GACCGA 1 cut(s) 98
TatI WGTACW 2 cut(s) 570, 1674
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 2 cut(s) 836, 1537
Tru1I TTAA 7 cut(s) 333, 803, 1043, 1089, 1119, 1268, 1455
Tru9I TTAA 7 cut(s) 333, 803, 1043, 1089, 1119, 1268, 1455
TscAI CASTG 2 cut(s) 156, 1244
TseFI GTSAC 3 cut(s) 147, 659, 1304
TseI GCWGC 7 cut(s) 19, 89, 404, 812, 995, 1290, 1859
Tsp45I GTSAC 3 cut(s) 147, 659, 1304
TspDTI ATGAA 4 cut(s) 17, 1352, 1462, 1572
TspGWI ACGGA 2 cut(s) 1085, 1707
TspRI CASTG 2 cut(s) 156, 1244
Tth111I GACNNNGTC 1 cut(s) 1847
Van91I CCANNNNNTGG 2 cut(s) 1852, 1888
Vha464I CTTAAG 2 cut(s) 332, 1454
VneI GTGCAC 2 cut(s) 1202, 1465
VpaK11BI GGWCC 4 cut(s) 110, 205, 309, 952
XapI RAATTY 4 cut(s) 733, 902, 1561, 1799
XceI RCATGY 2 cut(s) 45, 652
XcmI CCANNNNNNNNNTGG 2 cut(s) 368, 1499
XmiI GTMKAC 1 cut(s) 645
XmnI GAANNNNTTC 3 cut(s) 489, 887, 1802
XspI CTAG 3 cut(s) 353, 440, 633
ZraI GACGTC 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.