MD07G1057000.v1.1

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0020401
Physical Location & Seq
Forward (+)
5214085 .. 5216300
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1057000.v1.1.491

Sequence Viewer

Length: 825 bp
ATGTCTAGGAAGCTCGTGACGGTGTCTGCAGATACACACACTGACGGAGGAAACTCTGGACATGGTGGTACTCAAGTAGCTTTAATTATTGACGGCACCAGCCTCGTGTATATTCTTGACAGCGAACTTGAGGAAAAGCTCTTTGAACTAGCAAGTAACTGTGCTGTGGTGCTTTGTTGCCGAGTGGCTCCATTGCAAAAGGCTGGAATTGTAGCCCTTGTGAAGAACAGGACAACTGATATGACACTTGTCATTGGGGATGGTGCTAATGACGTTTCAATGATCCAAATGGCTGATGTGGGAGTTGGCATCAGTGGCCAAGAAGGTCAGCAAGCTGTCATGGCATCAGATTTTGCAATGGGGCAGTTCAGATTCTTAGTTCCCCTTTTATTGGTACATGGCCATTGGAATTACCAGCAGATGGGCTACATGATATTATACAATTTCTATAGGAATGCAGTGTTTGTTCTTATTTTGTTTTGGTACGTGCTCTTCACTTCTTTCACTTTGACAACTGCAATCACTGAATGGAGCAGCATGTTGTATTCTATAATCTACACTGCAGTACCTACAATTGATGCTATACCTTCCCTGGTTGGCTACTGGGCCATTTTTCAAGTCGCAAAGACTGCATCGTTTTGGTTATGTTTGTTGGCAATCGTCACAGGCGCGATCGCTCCACGCTTTGTTGTAAAGTTTTTGTATCAGTACTATAGACCTTGTGATGTTCAGATTGCAAGAGAATTTGAGAAGTTTGGCAATCCAAGCGCGTCAAACCCCGCACAAATAGAGATGGATGCGATCTTGGACCTGCGTAGGAGATGA

Protein Analysis

275

Amino Acids

30.37

Weight (kDa)

5.54

Isoelectric Point (pI)

27.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_C PF16212 113 - 192 7.6e-30 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020401)

Species Orthologous Gene IDs
malus_domestica MD07G1057000.v1.1
pyrus_communis pycom13g04470
rosa_laevigata RLG00000019012
rosa_rugosa Rorug02G0013500 Rorug03G0224600
rosa_samantha Rh5DG151200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 819
AccB1I GGYRCC 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 421
AccII CGCG 2 cut(s) 671, 770
AciI CCGC 1 cut(s) 780
AclWI GGATC 1 cut(s) 277
AcoI YGGCCR 2 cut(s) 316, 400
AcsI RAATTY 1 cut(s) 743
AfaI GTAC 5 cut(s) 70, 396, 485, 567, 710
AfiI CCNNNNNNNGG 2 cut(s) 391, 421
AgsI TTSAA 3 cut(s) 146, 279, 617
AjnI CCWGG 1 cut(s) 591
AluBI AGCT 4 cut(s) 13, 80, 139, 335
AluI AGCT 4 cut(s) 13, 80, 139, 335
Alw21I GWGCWC 1 cut(s) 492
AlwI GGATC 1 cut(s) 277
AoxI GGCC 3 cut(s) 316, 400, 606
ApeKI GCWGC 1 cut(s) 534
ApoI RAATTY 1 cut(s) 743
AsiSI GCGATCGC 1 cut(s) 675
AspLEI GCGC 2 cut(s) 671, 770
AspS9I GGNCC 2 cut(s) 606, 808
AvaII GGWCC 1 cut(s) 808
BaeI ACNNNNGTAYC 4 cut(s) 60, 93, 549, 582
BalI TGGCCA 2 cut(s) 318, 402
BanI GGYRCC 1 cut(s) 95
BarI GAAGNNNNNNTAC 2 cut(s) 476, 508
BauI CACGAG 2 cut(s) 14, 104
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 546
BccI CCATC 3 cut(s) 254, 415, 787
BceAI ACGGC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 1 cut(s) 593
BfaI CTAG 2 cut(s) 6, 149
BfmI CTRYAG 4 cut(s) 27, 448, 561, 712
BfuAI ACCTGC 1 cut(s) 819
BisI GCNGC 1 cut(s) 535
BlsI GCNGC 1 cut(s) 536
BmcAI AGTACT 1 cut(s) 710
Bme1390I CCNGG 1 cut(s) 593
Bme18I GGWCC 1 cut(s) 808
BmgT120I GGNCC 2 cut(s) 606, 808
BmiI GGNNCC 2 cut(s) 97, 189
BmrFI CCNGG 1 cut(s) 593
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 5 cut(s) 318, 353, 568, 641, 787
BmuI ACTGGG 1 cut(s) 613
BoxI GACNNNNGTC 1 cut(s) 248
BpuEI CTTGAG 2 cut(s) 57, 149
BsaAI YACGTR 1 cut(s) 487
BsaJI CCNNGG 1 cut(s) 591
Bsc4I CCNNNNNNNGG 2 cut(s) 391, 421
Bse1I ACTGG 1 cut(s) 608
Bse3DI GCAATG 2 cut(s) 191, 363
BseBI CCWGG 1 cut(s) 593
BseDI CCNNGG 1 cut(s) 591
BseGI GGATG 2 cut(s) 265, 802
BseLI CCNNNNNNNGG 2 cut(s) 391, 421
BseMI GCAATG 2 cut(s) 191, 363
BseNI ACTGG 1 cut(s) 608
BseXI GCAGC 1 cut(s) 546
Bsh1236I CGCG 2 cut(s) 671, 770
Bsh1285I CGRYCG 1 cut(s) 675
BshFI GGCC 3 cut(s) 318, 402, 608
BshNI GGYRCC 1 cut(s) 95
BsiEI CGRYCG 1 cut(s) 675
BsiHKAI GWGCWC 1 cut(s) 492
BslI CCNNNNNNNGG 2 cut(s) 391, 421
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 3 cut(s) 318, 402, 608
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 3 cut(s) 282, 672, 801
BspACI CCGC 1 cut(s) 780
BspANI GGCC 3 cut(s) 318, 402, 608
BspFNI CGCG 2 cut(s) 671, 770
BspLI GGNNCC 2 cut(s) 97, 189
BspMAI CTGCAG 2 cut(s) 31, 565
BspMI ACCTGC 1 cut(s) 819
BspPI GGATC 1 cut(s) 277
BspQI GCTCTTC 1 cut(s) 497
BspT107I GGYRCC 1 cut(s) 95
BsrDI GCAATG 2 cut(s) 191, 363
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 591
BssMI GATC 3 cut(s) 282, 672, 801
BssSI CACGAG 2 cut(s) 14, 104
Bst2BI CACGAG 2 cut(s) 14, 104
Bst2UI CCWGG 1 cut(s) 593
Bst4CI ACNGT 2 cut(s) 22, 161
Bst6I CTCTTC 1 cut(s) 497
BstAPI GCANNNNNTGC 1 cut(s) 629
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 376
BstF5I GGATG 2 cut(s) 265, 802
BstFNI CGCG 2 cut(s) 671, 770
BstHHI GCGC 2 cut(s) 671, 770
BstKTI GATC 3 cut(s) 285, 675, 804
BstMBI GATC 3 cut(s) 282, 672, 801
BstMCI CGRYCG 1 cut(s) 675
BstMWI GCNNNNNNNGC 4 cut(s) 315, 341, 629, 765
BstNI CCWGG 1 cut(s) 593
BstNSI RCATGY 1 cut(s) 541
BstPAI GACNNNNGTC 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 591
BstSFI CTRYAG 4 cut(s) 27, 448, 561, 712
BstUI CGCG 2 cut(s) 671, 770
BstV1I GCAGC 1 cut(s) 546
BsuRI GGCC 3 cut(s) 318, 402, 608
BtsCI GGATG 2 cut(s) 265, 802
BtsI GCAGTG 2 cut(s) 465, 558
BtsIMutI CAGTG 5 cut(s) 39, 319, 465, 522, 558
BveI ACCTGC 1 cut(s) 819
Cac8I GCNNGC 1 cut(s) 333
CfoI GCGC 2 cut(s) 671, 770
Cfr13I GGNCC 2 cut(s) 606, 808
CseI GACGC 1 cut(s) 759
Csp6I GTAC 5 cut(s) 69, 395, 484, 566, 709
CviAII CATG 5 cut(s) 62, 340, 398, 430, 538
CviQI GTAC 5 cut(s) 69, 395, 484, 566, 709
DdeI CTNAG 1 cut(s) 376
DpnI GATC 3 cut(s) 284, 674, 803
DpnII GATC 3 cut(s) 282, 672, 801
EaeI YGGCCR 2 cut(s) 316, 400
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 808
EcoRII CCWGG 1 cut(s) 591
FaeI CATG 5 cut(s) 65, 343, 401, 433, 541
FatI CATG 5 cut(s) 61, 339, 397, 429, 537
FauI CCCGC 1 cut(s) 787
Fnu4HI GCNGC 1 cut(s) 535
FokI GGATG 2 cut(s) 272, 809
Fsp4HI GCNGC 1 cut(s) 535
FspBI CTAG 2 cut(s) 6, 149
GlaI GCGC 2 cut(s) 670, 769
GluI GCNGC 1 cut(s) 535
HaeIII GGCC 3 cut(s) 318, 402, 608
HgaI GACGC 1 cut(s) 759
HhaI GCGC 2 cut(s) 671, 770
Hin1II CATG 5 cut(s) 65, 343, 401, 433, 541
Hin6I GCGC 2 cut(s) 669, 768
HinP1I GCGC 2 cut(s) 669, 768
HinfI GANTC 1 cut(s) 372
Hpy188I TCNGA 3 cut(s) 349, 371, 732
Hpy188III TCNNGA 3 cut(s) 16, 57, 116
HpyAV CCTTC 2 cut(s) 317, 597
HpyCH4III ACNGT 2 cut(s) 22, 161
HpyCH4IV ACGT 2 cut(s) 273, 486
HpyCH4V TGCA 8 cut(s) 29, 196, 356, 458, 518, 563, 632, 737
HpyF10VI GCNNNNNNNGC 4 cut(s) 315, 341, 629, 765
HpyF3I CTNAG 1 cut(s) 376
HpySE526I ACGT 2 cut(s) 273, 486
Hsp92II CATG 5 cut(s) 65, 343, 401, 433, 541
HspAI GCGC 2 cut(s) 669, 768
Kzo9I GATC 3 cut(s) 282, 672, 801
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 3 cut(s) 193, 531, 682
LpnPI CCDG 9 cut(s) 42, 112, 189, 214, 428, 578, 589, 605, 651
Lsp1109I GCAGC 1 cut(s) 546
LweI GCATC 5 cut(s) 318, 353, 568, 641, 787
MaeI CTAG 2 cut(s) 6, 149
MaeII ACGT 2 cut(s) 273, 486
MaeIII GTNAC 3 cut(s) 16, 155, 661
MalI GATC 3 cut(s) 284, 674, 803
MboI GATC 3 cut(s) 282, 672, 801
MboII GAAGA 2 cut(s) 235, 484
MfeI CAATTG 1 cut(s) 573
MhlI GDGCHC 1 cut(s) 492
MlsI TGGCCA 2 cut(s) 318, 402
MluCI AATT 6 cut(s) 84, 207, 409, 442, 573, 743
MluNI TGGCCA 2 cut(s) 318, 402
MnlI CCTC 3 cut(s) 41, 113, 124
Mox20I TGGCCA 2 cut(s) 318, 402
MscI TGGCCA 2 cut(s) 318, 402
MseI TTAA 1 cut(s) 83
Msp20I TGGCCA 2 cut(s) 318, 402
MspR9I CCNGG 1 cut(s) 593
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 1 cut(s) 460
MvaI CCWGG 1 cut(s) 593
MvnI CGCG 2 cut(s) 671, 770
MwoI GCNNNNNNNGC 4 cut(s) 315, 341, 629, 765
NdeII GATC 3 cut(s) 282, 672, 801
NlaIII CATG 5 cut(s) 65, 343, 401, 433, 541
NlaIV GGNNCC 2 cut(s) 97, 189
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 2 cut(s) 16, 661
NspI RCATGY 1 cut(s) 541
PciSI GCTCTTC 1 cut(s) 497
PctI GAATGC 1 cut(s) 460
PfeI GAWTC 1 cut(s) 372
PflFI GACNNNGTC 1 cut(s) 22
PflMI CCANNNNNTGG 1 cut(s) 421
PkrI GCNGC 1 cut(s) 536
Ple19I CGATCG 1 cut(s) 675
Ppu21I YACGTR 1 cut(s) 487
PshAI GACNNNNGTC 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 591
PspGI CCWGG 1 cut(s) 591
PspN4I GGNNCC 2 cut(s) 97, 189
PspPI GGNCC 2 cut(s) 606, 808
PstI CTGCAG 2 cut(s) 31, 565
PsyI GACNNNGTC 1 cut(s) 22
PvuI CGATCG 1 cut(s) 675
RgaI GCGATCGC 1 cut(s) 675
RsaI GTAC 5 cut(s) 70, 396, 485, 567, 710
RsaNI GTAC 5 cut(s) 69, 395, 484, 566, 709
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 1 cut(s) 535
Sau3AI GATC 3 cut(s) 282, 672, 801
Sau96I GGNCC 2 cut(s) 606, 808
ScaI AGTACT 1 cut(s) 710
ScrFI CCNGG 1 cut(s) 593
SduI GDGCHC 1 cut(s) 492
SfaAI GCGATCGC 1 cut(s) 675
SfaNI GCATC 5 cut(s) 318, 353, 568, 641, 787
SfcI CTRYAG 4 cut(s) 27, 448, 561, 712
SgfI GCGATCGC 1 cut(s) 675
SinI GGWCC 1 cut(s) 808
SmlI CTYRAG 2 cut(s) 72, 128
SmoI CTYRAG 2 cut(s) 72, 128
Sse9I AATT 6 cut(s) 84, 207, 409, 442, 573, 743
SsiI CCGC 1 cut(s) 780
SspMI CTAG 2 cut(s) 6, 149
StyD4I CCNGG 1 cut(s) 591
TaaI ACNGT 2 cut(s) 22, 161
TaiI ACGT 2 cut(s) 276, 489
TasI AATT 6 cut(s) 84, 207, 409, 442, 573, 743
TatI WGTACW 1 cut(s) 708
TfiI GAWTC 1 cut(s) 372
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 5 cut(s) 46, 319, 465, 529, 565
TseFI GTSAC 2 cut(s) 16, 661
TseI GCWGC 1 cut(s) 534
Tsp45I GTSAC 2 cut(s) 16, 661
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 5 cut(s) 46, 319, 465, 529, 565
Tth111I GACNNNGTC 1 cut(s) 22
Van91I CCANNNNNTGG 1 cut(s) 421
VpaK11BI GGWCC 1 cut(s) 808
XapI RAATTY 1 cut(s) 743
XceI RCATGY 1 cut(s) 541
XspI CTAG 2 cut(s) 6, 149
ZrmI AGTACT 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.