MD07G1165300.v1.1

3-phosphoinositide-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009841
Physical Location & Seq
Forward (+)
24018720 .. 24023252
4533 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1165300.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGTTGGCAATGGAGAAAGATTTTGATGCTAAGTTGACGATTCAGGGGAATTCTTCTAATGGCGCCAACAGCAATGTTCCGAGATCCAAGAGCTTCGCTTTCAGGGCTCCCCAGGAGCATTTCACCATTCAAGACTTTGAATTGGGCAAGATCTATGGCGTTGGTTCCTACTCCAAGGTTGTGAGGGCTAAGAAGAAGGACACGGGAACTGTCTATGCATTAAAGATCATGGACAAAAAATTCATCACCAAAGAAAATAAAACAGCTTATGTGAAGTTGGAACGTATTGTTCTTGATCAGCTGGATCATCCAGGAATTGTGCGGCTATTTTTTACATTTCAAGATTCCTTTTCATTGTACATGGCACTTGAGTCCTGTGAAGGTGGAGAACTTTTTGATCAAATAACTAGGAAAGGTCGTCTCTCTGAGGATGAAGCTTGCTTCTATGCAGCAGAAGTTGTAGACGCCCTTGAGTACATACACAGTATGGGATTGATTCATAGAGATATAAAGCCAGAGAACTTGCTACTTACATCAGAGGGTAATATCAAGATTGCTGATTTTGGGAGTGTAAAGCCCATGCAAGATAGCCGAATTACTGTTCTTCCGAATGCAGCATCAGATGATAAGGCCTGCACATTTGTGGGGACAGCTGCCTATGTTCCTCCCGAAGTCCTGAATTCGTCTCCTGCAACTTTCGGAAATGACCTCTGGGCACTTGGCTGCACCTTGTACCAAATGCTTTCAGGAACTTCACCTTTCAAAGATGCAAGCGAGTGGCTTATTTTCCAAAGAATTATAGCAAGAGATATTAGGTTTCCAAATTATTTTTCGGAGGAAGCTAAAGACCTTATTGACCGATTATTGGATTTAGATCCCAGCAGGAGACCAGGTGCTGGGCCGGACGGTTATGCTGCACTGAAGATGCATCCATTTTTTAAGGGAGTTGACTGGAAAAATGTACGAGGACAAACTCCTCCAAAACTTGCATTAGAGACGGGGTCAGTTGAGGGTGATAGCCGTGATAACTCATGGAACCCCACACACATTGGAGATGGTGCAGCAAAACAATATGAGGGTAACAGTGGTGCCACATCTTCTTCCGAAGGATCTGGTCATGTAACTAGGCTCGCTTCTATTGATTCCTTTGATTCAAAATGGCAACAATTCCTTGAGCCCGGGGAGTCCGTTCTTATGATCTCAATGGTGAAGAAAATTCAAAAGCTGACCAGCAAGAAGGTGCAGCTTATCCTCACCAACAAGCCGAAACTGATATATGTGGATCCTGCAAAGCTAATAGTGAAGGGAAATATAATTTGGTCCGACAACTCTAATGACTTCAGTATCCAAGTTGCTAGTCCATCACAGTTCAAGATTTGTACGCCGAAAAAGGTACTGCAATTTGAGGATGCCAAACAAAGAGCTTGGCAGTGGAAGAAGGCAATTGAGGGGCTTCAAAATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008289 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019220 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033674 GO:0035091 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0070300 GO:0071704 GO:0071944 GO:0080090 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko01524 ko03320 ko04011 ko04068 ko04071 ko04140 ko04150 ko04151 ko04152 ko04210 ko04510 ko04660 ko04664 ko04722 ko04910 ko04919 ko04931 ko04960 ko05145 ko05160 ko05205 ko05213 ko05215 ko05223 ko05231 map01524 map03320 map04011 map04068 map04071 map04140 map04150 map04151 map04152 map04210 map04510 map04660 map04664 map04722 map04910 map04919 map04931 map04960 map05145 map05160 map05205 map05213 map05215 map05223 map05231
Pfam Domains
Protein Families
PH_3 Pkinase

Protein Analysis

489

Amino Acids

54.45

Weight (kDa)

8.06

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 47 - 312 8.5e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 48 - 297 2.3e-30 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 151 - 262 1.4e-08 Kinase-like
PH_3 PF14593 385 - 487 1.5e-30 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009841)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10540 AT5G04510 AT5G04510 AT5G04510
fragaria_vesca FvH4_7g18280 FvH4_7g18280 FvH4_7g18280
malus_domestica MD07G1165300.v1.1
prunus_persica Prupe.2G204000_v2.0.a1 Prupe.2G204000_v2.0.a1
pyrus_communis pycom07g16060
rosa_chinensis RchiOBHm_Chr1g0361861
rosa_laevigata RLG00000027696
rosa_multiflora Rmu_ssc0000124.1_g000014
rosa_roxburghii Rroxscaffold_4G00294640
rosa_rugosa Rorug01G0292000
rosa_samantha Rh1AG302600 Rh1BG265700 Rh1CG283800 Rh1DG295700
rosa_wichuraiana Rw1G026810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 62, 1088
AccB7I CCANNNNNTGG 1 cut(s) 896
AccI GTMKAC 1 cut(s) 462
AciI CCGC 1 cut(s) 322
AclWI GGATC 6 cut(s) 78, 312, 869, 1117, 1277, 1290
AcsI RAATTY 4 cut(s) 49, 239, 679, 1215
AcuI CTGAAG 2 cut(s) 941, 1324
AcyI GRCGYC 2 cut(s) 63, 465
AfaI GTAC 6 cut(s) 359, 476, 734, 963, 1381, 1395
AfiI CCNNNNNNNGG 2 cut(s) 865, 896
AgsI TTSAA 8 cut(s) 131, 140, 341, 763, 1155, 1220, 1372, 1457
AjnI CCWGG 3 cut(s) 111, 310, 889
AjuI GAANNNNNNNTTGG 4 cut(s) 814, 846, 1300, 1332
AleI CACNNNNGTG 1 cut(s) 641
Alw26I GTCTC 4 cut(s) 425, 690, 880, 989
AlwI GGATC 6 cut(s) 78, 312, 869, 1117, 1277, 1290
AlwNI CAGNNNCTG 1 cut(s) 896
Ama87I CYCGRG 1 cut(s) 1178
AoxI GGCC 2 cut(s) 630, 899
ApeKI GCWGC 7 cut(s) 449, 614, 653, 723, 914, 1061, 1243
ApoI RAATTY 4 cut(s) 49, 239, 679, 1215
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 899, 1320
AsuC2I CCSGG 2 cut(s) 1179, 1180
AsuHPI GGTGA 6 cut(s) 115, 238, 747, 1025, 1219, 1246
AvaI CYCGRG 1 cut(s) 1178
AvaII GGWCC 1 cut(s) 1320
BaeGI GKGCMC 1 cut(s) 718
BaeI ACNNNNGTAYC 2 cut(s) 1327, 1360
BamHI GGATCC 1 cut(s) 1282
BanI GGYRCC 2 cut(s) 62, 1088
BanII GRGCYC 2 cut(s) 109, 1179
BbvI GCAGC 7 cut(s) 461, 626, 640, 710, 901, 1073, 1255
BccI CCATC 2 cut(s) 1049, 1369
BceAI ACGGC 1 cut(s) 1005
BciT130I CCWGG 3 cut(s) 113, 312, 891
BciVI GTATCC 1 cut(s) 1355
BclI TGATCA 2 cut(s) 295, 397
BcnI CCSGG 2 cut(s) 1179, 1180
BcoDI GTCTC 4 cut(s) 425, 690, 880, 989
BfaI CTAG 3 cut(s) 408, 1125, 1356
BfoI RGCGCY 1 cut(s) 66
BfuI GTATCC 1 cut(s) 1355
BglII AGATCT 1 cut(s) 150
BisI GCNGC 8 cut(s) 323, 450, 615, 654, 724, 915, 1062, 1244
BlsI GCNGC 8 cut(s) 324, 451, 616, 655, 725, 916, 1063, 1245
Bme1390I CCNGG 5 cut(s) 113, 312, 891, 1179, 1180
Bme18I GGWCC 1 cut(s) 1320
BmeT110I CYCGRG 1 cut(s) 1178
BmgT120I GGNCC 2 cut(s) 899, 1320
BmiI GGNNCC 6 cut(s) 64, 108, 166, 1037, 1090, 1284
BmrFI CCNGG 5 cut(s) 113, 312, 891, 1179, 1180
BmsI GCATC 6 cut(s) 16, 626, 757, 915, 937, 1399
BpuEI CTTGAG 3 cut(s) 389, 491, 1193
BpuMI CCSGG 2 cut(s) 1179, 1180
BsaBI GATNNNNATC 1 cut(s) 873
BsaHI GRCGYC 2 cut(s) 63, 465
BsaI GGTCTC 1 cut(s) 880
BsaJI CCNNGG 4 cut(s) 111, 174, 1178, 1179
BsaXI ACNNNNNCTCC 2 cut(s) 877, 907
Bsc4I CCNNNNNNNGG 2 cut(s) 865, 896
Bse1I ACTGG 1 cut(s) 956
Bse3DI GCAATG 2 cut(s) 15, 79
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 3 cut(s) 113, 312, 891
BseDI CCNNGG 4 cut(s) 111, 174, 1178, 1179
BseGI GGATG 4 cut(s) 307, 436, 928, 1414
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 2 cut(s) 865, 896
BseMI GCAATG 2 cut(s) 15, 79
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 956
BseRI GAGGAG 1 cut(s) 966
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 7 cut(s) 461, 626, 640, 710, 901, 1073, 1255
BseYI CCCAGC 2 cut(s) 878, 896
BsgI GTGCAG 5 cut(s) 619, 709, 900, 1080, 1262
BshFI GGCC 2 cut(s) 632, 901
BshNI GGYRCC 2 cut(s) 62, 1088
BsiHKCI CYCGRG 1 cut(s) 1178
BsiSI CCGG 2 cut(s) 902, 1179
BslFI GGGAC 1 cut(s) 661
BslI CCNNNNNNNGG 2 cut(s) 865, 896
BsmAI GTCTC 4 cut(s) 425, 690, 880, 989
BsmBI CGTCTC 3 cut(s) 425, 690, 989
BsmFI GGGAC 1 cut(s) 661
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 2 cut(s) 632, 901
Bso31I GGTCTC 1 cut(s) 880
BsoBI CYCGRG 1 cut(s) 1178
Bsp1286I GDGCHC 3 cut(s) 109, 718, 1179
Bsp1407I TGTACA 1 cut(s) 357
BspACI CCGC 1 cut(s) 322
BspANI GGCC 2 cut(s) 632, 901
BspCNI CTCAG 1 cut(s) 418
BspLI GGNNCC 6 cut(s) 64, 108, 166, 1037, 1090, 1284
BspPI GGATC 6 cut(s) 78, 312, 869, 1117, 1277, 1290
BspT107I GGYRCC 2 cut(s) 62, 1088
BspTNI GGTCTC 1 cut(s) 880
BsrDI GCAATG 2 cut(s) 15, 79
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 1 cut(s) 956
BssECI CCNNGG 4 cut(s) 111, 174, 1178, 1179
BssNI GRCGYC 2 cut(s) 63, 465
BssT1I CCWWGG 1 cut(s) 174
Bst2UI CCWGG 3 cut(s) 113, 312, 891
Bst4CI ACNGT 6 cut(s) 211, 485, 601, 908, 1085, 1368
BstACI GRCGYC 2 cut(s) 63, 465
BstAUI TGTACA 1 cut(s) 357
BstC8I GCNNGC 4 cut(s) 439, 634, 772, 1131
BstDEI CTNAG 3 cut(s) 30, 189, 426
BstF5I GGATG 4 cut(s) 307, 436, 928, 1414
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 1 cut(s) 65
BstMAI GTCTC 4 cut(s) 425, 690, 880, 989
BstMWI GCNNNNNNNGC 2 cut(s) 69, 104
BstNI CCWGG 3 cut(s) 113, 312, 891
BstSCI CCNGG 5 cut(s) 111, 310, 889, 1177, 1178
BstSLI GKGCMC 1 cut(s) 718
BstV1I GCAGC 7 cut(s) 461, 626, 640, 710, 901, 1073, 1255
BstX2I RGATCY 5 cut(s) 83, 150, 874, 1109, 1282
BstYI RGATCY 5 cut(s) 83, 150, 874, 1109, 1282
BsuI GTATCC 1 cut(s) 1355
BsuRI GGCC 2 cut(s) 632, 901
BtsCI GGATG 4 cut(s) 307, 436, 928, 1414
BtsI GCAGTG 1 cut(s) 1436
BtsIMutI CAGTG 3 cut(s) 917, 1090, 1436
Cac8I GCNNGC 4 cut(s) 439, 634, 772, 1131
CaiI CAGNNNCTG 1 cut(s) 896
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 899, 1320
Cfr9I CCCGGG 1 cut(s) 1178
CseI GACGC 1 cut(s) 473
CsiI ACCWGGT 1 cut(s) 889
Csp6I GTAC 6 cut(s) 358, 475, 733, 962, 1380, 1394
CspCI CAANNNNNGTGG 2 cut(s) 1030, 1065
CviAII CATG 5 cut(s) 229, 361, 580, 1032, 1118
CviQI GTAC 6 cut(s) 358, 475, 733, 962, 1380, 1394
DdeI CTNAG 3 cut(s) 30, 189, 426
DinI GGCGCC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 632
Eco24I GRGCYC 2 cut(s) 109, 1179
Eco31I GGTCTC 1 cut(s) 880
Eco47I GGWCC 1 cut(s) 1320
Eco57I CTGAAG 2 cut(s) 941, 1324
Eco88I CYCGRG 1 cut(s) 1178
EcoRI GAATTC 2 cut(s) 49, 679
EcoRII CCWGG 3 cut(s) 111, 310, 889
EcoT14I CCWWGG 1 cut(s) 174
EcoT22I ATGCAT 2 cut(s) 220, 930
EcoT38I GRGCYC 2 cut(s) 109, 1179
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 174
Esp3I CGTCTC 3 cut(s) 425, 690, 989
FaeI CATG 5 cut(s) 232, 364, 583, 1035, 1121
FaqI GGGAC 1 cut(s) 661
FatI CATG 5 cut(s) 228, 360, 579, 1031, 1117
FbaI TGATCA 2 cut(s) 295, 397
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 8 cut(s) 323, 450, 615, 654, 724, 915, 1062, 1244
FokI GGATG 4 cut(s) 294, 443, 915, 1421
FriOI GRGCYC 2 cut(s) 109, 1179
Fsp4HI GCNGC 8 cut(s) 323, 450, 615, 654, 724, 915, 1062, 1244
FspBI CTAG 3 cut(s) 408, 1125, 1356
GlaI GCGC 1 cut(s) 64
GluI GCNGC 8 cut(s) 323, 450, 615, 654, 724, 915, 1062, 1244
GsaI CCCAGC 2 cut(s) 882, 900
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 2 cut(s) 632, 901
HapII CCGG 2 cut(s) 902, 1179
HgaI GACGC 1 cut(s) 473
HhaI GCGC 1 cut(s) 65
Hin1I GRCGYC 2 cut(s) 63, 465
Hin1II CATG 5 cut(s) 232, 364, 583, 1035, 1121
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HincII GTYRAC 2 cut(s) 36, 949
HindII GTYRAC 2 cut(s) 36, 949
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 7 cut(s) 40, 344, 371, 496, 1142, 1151, 1184
HpaII CCGG 2 cut(s) 902, 1179
HphI GGTGA 6 cut(s) 115, 238, 747, 1025, 1219, 1246
Hpy166II GTNNAC 3 cut(s) 36, 463, 949
Hpy188I TCNGA 9 cut(s) 81, 427, 538, 609, 622, 701, 835, 1105, 1324
Hpy188III TCNNGA 8 cut(s) 131, 293, 341, 550, 668, 676, 747, 1372
Hpy8I GTNNAC 3 cut(s) 36, 463, 949
HpyAV CCTTC 6 cut(s) 190, 374, 1100, 1231, 1297, 1432
HpyCH4III ACNGT 6 cut(s) 211, 485, 601, 908, 1085, 1368
HpyCH4IV ACGT 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 104
HpyF3I CTNAG 3 cut(s) 30, 189, 426
HpySE526I ACGT 1 cut(s) 283
Hsp92I GRCGYC 2 cut(s) 63, 465
Hsp92II CATG 5 cut(s) 232, 364, 583, 1035, 1121
HspAI GCGC 1 cut(s) 63
KasI GGCGCC 1 cut(s) 62
Ksp22I TGATCA 2 cut(s) 295, 397
LmnI GCTCC 2 cut(s) 112, 115
Lsp1109I GCAGC 7 cut(s) 461, 626, 640, 710, 901, 1073, 1255
LweI GCATC 6 cut(s) 16, 626, 757, 915, 937, 1399
MabI ACCWGGT 1 cut(s) 889
MaeI CTAG 3 cut(s) 408, 1125, 1356
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 2 cut(s) 1079, 1120
MboII GAAGA 8 cut(s) 45, 205, 596, 934, 1089, 1092, 1222, 1447
MfeI CAATTG 1 cut(s) 1443
MflI RGATCY 5 cut(s) 83, 150, 874, 1109, 1282
MhlI GDGCHC 3 cut(s) 109, 718, 1179
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 2 cut(s) 380, 1193
MmeI TCCRAC 2 cut(s) 258, 1347
Mph1103I ATGCAT 2 cut(s) 220, 930
MseI TTAA 2 cut(s) 221, 939
MslI CAYNNNNRTG 1 cut(s) 641
MspA1I CMGCKG 2 cut(s) 301, 653
MspI CCGG 2 cut(s) 902, 1179
MspR9I CCNGG 5 cut(s) 113, 312, 891, 1179, 1180
MunI CAATTG 1 cut(s) 1443
Mva1269I GAATGC 1 cut(s) 616
MvaI CCWGG 3 cut(s) 113, 312, 891
MwoI GCNNNNNNNGC 2 cut(s) 69, 104
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 2 cut(s) 1179, 1180
NlaIII CATG 5 cut(s) 232, 364, 583, 1035, 1121
NlaIV GGNNCC 6 cut(s) 64, 108, 166, 1037, 1090, 1284
NsiI ATGCAT 2 cut(s) 220, 930
OliI CACNNNNGTG 1 cut(s) 641
PceI AGGCCT 1 cut(s) 632
PctI GAATGC 1 cut(s) 616
PfeI GAWTC 5 cut(s) 40, 344, 496, 1142, 1151
PflFI GACNNNGTC 1 cut(s) 1000
PflMI CCANNNNNTGG 1 cut(s) 896
PfoI TCCNGGA 1 cut(s) 310
PkrI GCNGC 8 cut(s) 324, 451, 616, 655, 725, 916, 1063, 1245
PleI GAGTC 2 cut(s) 379, 1192
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 2 cut(s) 379, 1192
Psp6I CCWGG 3 cut(s) 111, 310, 889
PspFI CCCAGC 2 cut(s) 878, 896
PspGI CCWGG 3 cut(s) 111, 310, 889
PspN4I GGNNCC 6 cut(s) 64, 108, 166, 1037, 1090, 1284
PspPI GGNCC 2 cut(s) 899, 1320
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 5 cut(s) 83, 150, 874, 1109, 1282
PsyI GACNNNGTC 1 cut(s) 1000
PvuII CAGCTG 2 cut(s) 301, 653
RsaI GTAC 6 cut(s) 359, 476, 734, 963, 1381, 1395
RsaNI GTAC 6 cut(s) 358, 475, 733, 962, 1380, 1394
RseI CAYNNNNRTG 1 cut(s) 641
SaqAI TTAA 2 cut(s) 221, 939
SatI GCNGC 8 cut(s) 323, 450, 615, 654, 724, 915, 1062, 1244
Sau96I GGNCC 2 cut(s) 899, 1320
SchI GAGTC 2 cut(s) 380, 1193
ScrFI CCNGG 5 cut(s) 113, 312, 891, 1179, 1180
SduI GDGCHC 3 cut(s) 109, 718, 1179
SexAI ACCWGGT 1 cut(s) 889
SfaNI GCATC 6 cut(s) 16, 626, 757, 915, 937, 1399
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 1 cut(s) 1320
SmaI CCCGGG 1 cut(s) 1180
SmiMI CAYNNNNRTG 1 cut(s) 641
SmlI CTYRAG 3 cut(s) 368, 470, 1172
SmoI CTYRAG 3 cut(s) 368, 470, 1172
SseBI AGGCCT 1 cut(s) 632
SsiI CCGC 1 cut(s) 322
SspDI GGCGCC 1 cut(s) 62
SspMI CTAG 3 cut(s) 408, 1125, 1356
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 5 cut(s) 111, 310, 889, 1177, 1178
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 6 cut(s) 211, 485, 601, 908, 1085, 1368
TaiI ACGT 1 cut(s) 286
TaqII GACCGA 1 cut(s) 873
TatI WGTACW 2 cut(s) 357, 474
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 5 cut(s) 40, 344, 496, 1142, 1151
Tru1I TTAA 2 cut(s) 221, 939
Tru9I TTAA 2 cut(s) 221, 939
TscAI CASTG 3 cut(s) 924, 1090, 1436
TseI GCWGC 7 cut(s) 449, 614, 653, 723, 914, 1061, 1243
TspDTI ATGAA 4 cut(s) 232, 342, 447, 488
TspGWI ACGGA 1 cut(s) 1177
TspMI CCCGGG 1 cut(s) 1178
TspRI CASTG 3 cut(s) 924, 1090, 1436
Tth111I GACNNNGTC 1 cut(s) 1000
Van91I CCANNNNNTGG 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 1320
XapI RAATTY 4 cut(s) 49, 239, 679, 1215
XmaI CCCGGG 1 cut(s) 1178
XmiI GTMKAC 1 cut(s) 462
XspI CTAG 3 cut(s) 408, 1125, 1356
Zsp2I ATGCAT 2 cut(s) 220, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.