Rroxscaffold_4G00294640

3-phosphoinositide-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009841
Physical Location & Seq
Reverse (-)
14667433 .. 14673896
6464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294640.1

Sequence Viewer

Length: 2382 bp
ATGTTGGCAATGGAGAAAGATTTTGATGCCAAGTTGAAGATTCAGGGGAATTCTTCTTCTAGTGGTGGTGGTGGTGGTGGTGCTAATAGTAATGTTCAGAGAGCCAAGAACTTTGCTTTCAGGGCTCCCCAAGAGAATTTCACCATCCAAGACTTTGAATTGGGCAAGATCTATGGCGTTGGTTCCTATTCAAAGGTTGTGAGAGCAAGGAAGAAGGATTCAGGAATTGTCTACGCATTAAAGATCATGGACAAAAAGTTCATCACCAAAGAAAATAAAACAGCTTATGTGAAGCTGGAACGTATTGTTCTTGATCAGCTGGATCATCCAGGTGTTGTGCGGCTATTTTTCACTTTTCAGGATTCCTTTTCACTGTATATGGCACTGGAATCCTGTGAAGGTGGAGAACTCTTTGATCAAATAACAAGGAAAGGTCGTCTGTCTGAGGACGAAGCTCGCTTTTATGCAGCAGAAGTTGTAGACGCACTTGAATATATACACAGTATGGGATTGATACATAGAGATATAAAGCCAGAGAATTTGTTACTTACAGCAGAGGGTAATATCAAGGTTGCTGATTTTGGGAGTGTAAAGCCTATGCAGGATAGCCAAATTACAGTCCTTCCAAATGCAGCATCAGATGATAAGGCTTGTACATTTGTTGGGACAGCTGCATATGTTCCTCCAGAAGTCCTGAATTCTTCCCCTGCAACTTTTGGAAATGACCTCTGGGCACTTGGCTGCACCTTGTACCAAATGCTTTCAGGAACTTCTCCTTTCAAAGATGCAAGCGAGTGGCTTATTTTTCAAAGAATTATAGCAAGAGATATTAGGTTTCCAAATTATTTCTCAGACGAAGCTAAAGACCTTATTGATCAGTTATTGGATTTAGATCCCAGTAGGAGGCCTGGTGCTGGACGCGATGGTTATGCTGCACTGAAGATGCATCCATTCTTTAAGGGGGTCGACTGGAAAAATTTAAGAGCGCAAACCCCTCCGAAACTTGCCTTGGAGCCGGGGTCAGGTGAGGCTGATGATGTCCGTGATAACTCGTGGAACCCTTCACATATTGGAGATGGCGCAGCAAGACAAAATGAGGGTAACACTGGTTCAGCATCCTCTTCTGAAGCATCTGGTCATGTAACTAGGCTAGCTTCAATTGACTCCTTTGATTCAAAATGGCAACAATTCCTTGACCCTGGTGAGTCTGTTCTCATGATCTCAATGGTGAAGAAACTTCAAAAACTGACAAGCAAGAAGGTGCAGCTTATTCTCACCAACAAGCCAAAGTTGATATATGTGGATCCTGCAAAGCTGCTGATGAAGGGAAATATAATTTGGTCTGACAACTCTAATGATCTCAATGTCCAAGTTGCTAGTCCTTCACAGTTCAAAATTGTTACGCCCAAAAAGGTACTGTCATTTGAGGATGCAAAACAAAGAGCATGGCAATGGAAGAAGGCAATTGAGGGGCTTCAAAATAGGAGATCTTTGAAGCTCATCCAAGAAATAAACCGGAAGGGACCTTACATCGGCCTCATCACAGTCTATCCACCAGAAGAGGCTGCTTTTTTCCACACCGGGGCTTTTAAGCCTAATCCAAAGCATCCTTTTCTGGATTTCTCAGGTAGGCGATTCCGGGTGGGGAAGATCCATGATAAGAAAGTCGTCTATGTGAGATGTGGAGTTGGAATGGTGAATGCTGCTGCAGCAACACAGCAAATGCTGGATCTATTTGACGTTGTCGGAATTGTTCACTTTGGCATATCTGGCAATGCCAATAACTCTATGTCTATTGGTGATGTTACCATTCCTCAACAATTAGCTCACACTGGCATATGGGATTGGGTGGATGTTGCACAATTAGACTTTGAAGTATACAATGAACCAAAAGGAGGAGAAAACCTGTTGGGACACATTGGATTTAGAAATGAACAGTTCTATTCTGAGTCTGGTAATCCAAATATCGCTCGGCCATTGTTTTGGGCAAAGACTACTCGAAAGTGGCTTCAGGTTGCTGCCCATTTGGAGGGAGTCGAACTGGAGCAATGTGTGAATTCGAGTTTGTGTCTGCCACAGAAGCCCAAGCTAGTTGTTGGTCTTAGGGGATCAACAGGCAACACTTTTGTGGACAATGCAGCTTACAGGAACTTCCTTTTTCAAACTTTTCATGTTTCCTCAGTGGACATGGAAAGCTCGGCTGTAGTAATGACAAGCTTGTCAAATGGCTTCCCGGTGATTGTGTTCAGAGGGTTATCAGATTTAGCAGGAAGGCAAGCAGGAGAGAACCCGATTCAGACCTTTGGGCCTCTGGCAGCTATAAATGCTTGCAAAGTAGTTGTACAGTTCATCAAGGACCTGCCAGAGGCAGGGTATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008289 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019220 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033674 GO:0035091 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0070300 GO:0071704 GO:0071944 GO:0080090 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko01524 ko03320 ko04011 ko04068 ko04071 ko04140 ko04150 ko04151 ko04152 ko04210 ko04510 ko04660 ko04664 ko04722 ko04910 ko04919 ko04931 ko04960 ko05145 ko05160 ko05205 ko05213 ko05215 ko05223 ko05231 map01524 map03320 map04011 map04068 map04071 map04140 map04150 map04151 map04152 map04210 map04510 map04660 map04664 map04722 map04910 map04919 map04931 map04960 map05145 map05160 map05205 map05213 map05215 map05223 map05231
Pfam Domains
Protein Families
PH_3 Pkinase

Protein Analysis

793

Amino Acids

87.7

Weight (kDa)

8.21

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 53 - 318 1.4e-65 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 54 - 303 4.5e-29 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 150 - 266 1.2e-08 Kinase-like
PH_3 PF14593 392 - 495 3.7e-30 PH domain
PNP_UDP_1 PF01048 511 - 785 3.3e-31 Phosphorylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009841)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10540 AT5G04510 AT5G04510 AT5G04510
fragaria_vesca FvH4_7g18280 FvH4_7g18280 FvH4_7g18280
malus_domestica MD07G1165300.v1.1
prunus_persica Prupe.2G204000_v2.0.a1 Prupe.2G204000_v2.0.a1
pyrus_communis pycom07g16060
rosa_chinensis RchiOBHm_Chr1g0361861
rosa_laevigata RLG00000027696
rosa_multiflora Rmu_ssc0000124.1_g000014
rosa_roxburghii Rroxscaffold_4G00294640
rosa_rugosa Rorug01G0292000
rosa_samantha Rh1AG302600 Rh1BG265700 Rh1CG283800 Rh1DG295700
rosa_wichuraiana Rw1G026810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2218
Acc36I ACCTGC 1 cut(s) 2367
AccI GTMKAC 4 cut(s) 231, 480, 966, 1878
AccII CGCG 1 cut(s) 921
AciI CCGC 1 cut(s) 340
AclWI GGATC 7 cut(s) 330, 887, 1298, 1311, 1645, 1737, 2116
AcoI YGGCCR 1 cut(s) 1973
AcsI RAATTY 6 cut(s) 49, 136, 538, 697, 976, 2056
AcuI CTGAAG 3 cut(s) 959, 1146, 1994
AfaI GTAC 4 cut(s) 655, 752, 1416, 2343
AfiI CCNNNNNNNGG 9 cut(s) 903, 914, 1022, 1532, 1582, 1895, 2029, 2365, 2369
AjnI CCWGG 3 cut(s) 328, 907, 1198
AjuI GAANNNNNNNTTGG 4 cut(s) 992, 1024, 1321, 1353
AleI CACNNNNGTG 1 cut(s) 2126
AloI GAACNNNNNNTCC 2 cut(s) 242, 274
AlwI GGATC 7 cut(s) 330, 887, 1298, 1311, 1645, 1737, 2116
AoxI GGCC 4 cut(s) 905, 1534, 1973, 2306
ApoI RAATTY 6 cut(s) 49, 136, 538, 697, 976, 2056
AspLEI GCGC 2 cut(s) 988, 1082
AspS9I GGNCC 3 cut(s) 1523, 2306, 2356
AsuC2I CCSGG 4 cut(s) 1017, 1582, 1640, 2234
AsuHPI GGTGA 9 cut(s) 133, 256, 1037, 1214, 1240, 1267, 1708, 1811, 2248
AsuNHI GCTAGC 1 cut(s) 1150
AvaII GGWCC 2 cut(s) 1523, 2356
BaeGI GKGCMC 1 cut(s) 736
BamHI GGATCC 1 cut(s) 1303
BanII GRGCYC 1 cut(s) 127
BarI GAAGNNNNNNTAC 2 cut(s) 1511, 1543
BauI CACGAG 1 cut(s) 1051
BccI CCATC 3 cut(s) 152, 917, 1070
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 3 cut(s) 330, 909, 1200
BclI TGATCA 3 cut(s) 313, 415, 874
BcnI CCSGG 4 cut(s) 1017, 1582, 1640, 2234
BfaI CTAG 5 cut(s) 60, 1146, 1151, 1377, 2090
BfmI CTRYAG 2 cut(s) 1707, 2202
BfuAI ACCTGC 1 cut(s) 2367
BglII AGATCT 2 cut(s) 168, 1487
Bme1390I CCNGG 7 cut(s) 330, 909, 1017, 1200, 1582, 1640, 2234
Bme18I GGWCC 2 cut(s) 1523, 2356
BmgT120I GGNCC 3 cut(s) 1523, 2306, 2356
BmiI GGNNCC 6 cut(s) 126, 184, 1014, 1058, 1305, 1524
BmrFI CCNGG 7 cut(s) 330, 909, 1017, 1200, 1582, 1640, 2234
BmrI ACTGGG 1 cut(s) 891
BmsI GCATC 9 cut(s) 16, 644, 775, 933, 955, 1124, 1139, 1420, 1615
BmtI GCTAGC 1 cut(s) 1154
BmuI ACTGGG 1 cut(s) 891
BpmI CTGGAG 2 cut(s) 669, 2063
BpuMI CCSGG 4 cut(s) 1017, 1582, 1640, 2234
BsaBI GATNNNNATC 1 cut(s) 891
BsaJI CCNNGG 4 cut(s) 1008, 1016, 1198, 1581
BsaWI WCCGGW 1 cut(s) 1515
BsaXI ACNNNNNCTCC 4 cut(s) 895, 925, 1004, 1034
Bsc4I CCNNNNNNNGG 9 cut(s) 903, 914, 1022, 1532, 1582, 1895, 2029, 2365, 2369
Bse1I ACTGG 6 cut(s) 390, 897, 974, 1111, 1837, 2046
Bse3DI GCAATG 4 cut(s) 15, 1457, 1780, 2054
Bse8I GATNNNNATC 1 cut(s) 891
BseBI CCWGG 3 cut(s) 330, 909, 1200
BseDI CCNNGG 4 cut(s) 1008, 1016, 1198, 1581
BseGI GGATG 8 cut(s) 144, 325, 946, 1115, 1435, 1500, 1606, 1858
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 9 cut(s) 903, 914, 1022, 1532, 1582, 1895, 2029, 2365, 2369
BseMI GCAATG 4 cut(s) 15, 1457, 1780, 2054
BseMII CTCAG 5 cut(s) 435, 864, 1638, 1938, 2193
BseNI ACTGG 6 cut(s) 390, 897, 974, 1111, 1837, 2046
BseRI GAGGAG 1 cut(s) 1911
BseSI GKGCMC 1 cut(s) 736
BsgI GTGCAG 3 cut(s) 727, 918, 1283
Bsh1236I CGCG 1 cut(s) 921
BshFI GGCC 4 cut(s) 907, 1536, 1975, 2308
BsiSI CCGG 5 cut(s) 1016, 1516, 1581, 1639, 2234
BslFI GGGAC 3 cut(s) 679, 1536, 1926
BslI CCNNNNNNNGG 9 cut(s) 903, 914, 1022, 1532, 1582, 1895, 2029, 2365, 2369
BsmFI GGGAC 3 cut(s) 679, 1536, 1926
BsmI GAATGC 1 cut(s) 1705
BsnI GGCC 4 cut(s) 907, 1536, 1975, 2308
Bsp1286I GDGCHC 2 cut(s) 127, 736
Bsp1407I TGTACA 2 cut(s) 653, 2341
BspACI CCGC 1 cut(s) 340
BspANI GGCC 4 cut(s) 907, 1536, 1975, 2308
BspCNI CTCAG 5 cut(s) 436, 863, 1637, 1939, 2192
BspFNI CGCG 1 cut(s) 921
BspHI TCATGA 1 cut(s) 1215
BspLI GGNNCC 6 cut(s) 126, 184, 1014, 1058, 1305, 1524
BspMAI CTGCAG 1 cut(s) 1711
BspMI ACCTGC 1 cut(s) 2367
BspOI GCTAGC 1 cut(s) 1154
BspPI GGATC 7 cut(s) 330, 887, 1298, 1311, 1645, 1737, 2116
BsrDI GCAATG 4 cut(s) 15, 1457, 1780, 2054
BsrGI TGTACA 2 cut(s) 653, 2341
BsrI ACTGG 6 cut(s) 390, 897, 974, 1111, 1837, 2046
BssECI CCNNGG 4 cut(s) 1008, 1016, 1198, 1581
BssNAI GTATAC 1 cut(s) 1879
BssSI CACGAG 1 cut(s) 1051
BssT1I CCWWGG 1 cut(s) 1008
Bst1107I GTATAC 1 cut(s) 1879
Bst2BI CACGAG 1 cut(s) 1051
Bst2UI CCWGG 3 cut(s) 330, 909, 1200
Bst4CI ACNGT 8 cut(s) 375, 503, 619, 1389, 1419, 1546, 1938, 2346
Bst6I CTCTTC 2 cut(s) 1126, 1554
BstAUI TGTACA 2 cut(s) 653, 2341
BstC8I GCNNGC 5 cut(s) 457, 790, 1152, 2277, 2329
BstDEI CTNAG 6 cut(s) 444, 850, 1624, 1947, 2102, 2179
BstENI CCTNNNNNAGG 1 cut(s) 2363
BstF5I GGATG 8 cut(s) 144, 325, 946, 1115, 1435, 1500, 1606, 1858
BstFNI CGCG 1 cut(s) 921
BstHHI GCGC 2 cut(s) 988, 1082
BstMWI GCNNNNNNNGC 5 cut(s) 122, 1709, 1770, 2080, 2324
BstNI CCWGG 3 cut(s) 330, 909, 1200
BstSCI CCNGG 7 cut(s) 328, 907, 1015, 1198, 1580, 1638, 2232
BstSFI CTRYAG 2 cut(s) 1707, 2202
BstSLI GKGCMC 1 cut(s) 736
BstUI CGCG 1 cut(s) 921
BstX2I RGATCY 6 cut(s) 168, 892, 1303, 1487, 1650, 1729
BstXI CCANNNNNNTGG 1 cut(s) 1983
BstYI RGATCY 6 cut(s) 168, 892, 1303, 1487, 1650, 1729
BstZ17I GTATAC 1 cut(s) 1879
BsuRI GGCC 4 cut(s) 907, 1536, 1975, 2308
BtgZI GCGATG 1 cut(s) 936
BtsCI GGATG 8 cut(s) 144, 325, 946, 1115, 1435, 1500, 1606, 1858
BtsIMutI CAGTG 6 cut(s) 371, 383, 935, 1104, 1830, 2187
BveI ACCTGC 1 cut(s) 2367
Cac8I GCNNGC 5 cut(s) 457, 790, 1152, 2277, 2329
CciI TCATGA 1 cut(s) 1215
CfoI GCGC 2 cut(s) 988, 1082
Cfr13I GGNCC 3 cut(s) 1523, 2306, 2356
CseI GACGC 2 cut(s) 491, 927
Csp6I GTAC 4 cut(s) 654, 751, 1415, 2342
CviAII CATG 7 cut(s) 247, 1139, 1216, 1446, 1655, 2171, 2188
CviQI GTAC 4 cut(s) 654, 751, 1415, 2342
DdeI CTNAG 6 cut(s) 444, 850, 1624, 1947, 2102, 2179
DrdI GACNNNNNNGTC 1 cut(s) 2218
DseDI GACNNNNNNGTC 1 cut(s) 2218
EaeI YGGCCR 1 cut(s) 1973
Eam1104I CTCTTC 2 cut(s) 1126, 1554
EarI CTCTTC 2 cut(s) 1126, 1554
Eco130I CCWWGG 1 cut(s) 1008
Eco147I AGGCCT 1 cut(s) 907
Eco24I GRGCYC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 1523, 2356
Eco57I CTGAAG 3 cut(s) 959, 1146, 1994
EcoNI CCTNNNNNAGG 1 cut(s) 2363
EcoO109I RGGNCCY 2 cut(s) 1523, 2356
EcoRI GAATTC 3 cut(s) 49, 697, 2056
EcoRII CCWGG 3 cut(s) 328, 907, 1198
EcoT14I CCWWGG 1 cut(s) 1008
EcoT22I ATGCAT 1 cut(s) 948
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 1008
FaeI CATG 7 cut(s) 250, 1142, 1219, 1449, 1658, 2174, 2191
FaqI GGGAC 3 cut(s) 679, 1536, 1926
FatI CATG 7 cut(s) 246, 1138, 1215, 1445, 1654, 2170, 2187
FauNDI CATATG 2 cut(s) 676, 1838
FbaI TGATCA 3 cut(s) 313, 415, 874
FblI GTMKAC 4 cut(s) 231, 480, 966, 1878
FokI GGATG 8 cut(s) 131, 312, 933, 1102, 1442, 1487, 1593, 1865
FriOI GRGCYC 1 cut(s) 127
FspBI CTAG 5 cut(s) 60, 1146, 1151, 1377, 2090
GlaI GCGC 2 cut(s) 987, 1081
GsuI CTGGAG 2 cut(s) 669, 2063
HaeIII GGCC 4 cut(s) 907, 1536, 1975, 2308
HapII CCGG 5 cut(s) 1016, 1516, 1581, 1639, 2234
HgaI GACGC 2 cut(s) 491, 927
HhaI GCGC 2 cut(s) 988, 1082
Hin1II CATG 7 cut(s) 250, 1142, 1219, 1449, 1658, 2174, 2191
Hin6I GCGC 2 cut(s) 986, 1080
HinP1I GCGC 2 cut(s) 986, 1080
HincII GTYRAC 1 cut(s) 967
HindII GTYRAC 1 cut(s) 967
HindIII AAGCTT 1 cut(s) 2215
HpaII CCGG 5 cut(s) 1016, 1516, 1581, 1639, 2234
HphI GGTGA 9 cut(s) 133, 256, 1037, 1214, 1240, 1267, 1708, 1811, 2248
Hpy166II GTNNAC 7 cut(s) 232, 481, 967, 1756, 1879, 2131, 2185
Hpy188III TCNNGA 8 cut(s) 222, 311, 359, 686, 694, 765, 1216, 1616
Hpy8I GTNNAC 7 cut(s) 232, 481, 967, 1756, 1879, 2131, 2185
HpyCH4III ACNGT 8 cut(s) 375, 503, 619, 1389, 1419, 1546, 1938, 2346
HpyCH4IV ACGT 2 cut(s) 301, 1740
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 1709, 1770, 2080, 2324
HpyF3I CTNAG 6 cut(s) 444, 850, 1624, 1947, 2102, 2179
HpySE526I ACGT 2 cut(s) 301, 1740
Hsp92II CATG 7 cut(s) 250, 1142, 1219, 1449, 1658, 2174, 2191
HspAI GCGC 2 cut(s) 986, 1080
Ksp22I TGATCA 3 cut(s) 313, 415, 874
LmnI GCTCC 3 cut(s) 130, 1012, 2044
LweI GCATC 9 cut(s) 16, 644, 775, 933, 955, 1124, 1139, 1420, 1615
MaeI CTAG 5 cut(s) 60, 1146, 1151, 1377, 2090
MaeII ACGT 2 cut(s) 301, 1740
MaeIII GTNAC 5 cut(s) 543, 1100, 1141, 1399, 1804
MfeI CAATTG 2 cut(s) 1158, 1464
MflI RGATCY 6 cut(s) 168, 892, 1303, 1487, 1650, 1729
MhlI GDGCHC 2 cut(s) 127, 736
MlyI GAGTC 4 cut(s) 1157, 1214, 1958, 2043
MmeI TCCRAC 2 cut(s) 1669, 1726
Mph1103I ATGCAT 1 cut(s) 948
MseI TTAA 4 cut(s) 239, 957, 980, 1590
MslI CAYNNNNRTG 3 cut(s) 330, 1450, 2126
MspA1I CMGCKG 2 cut(s) 319, 671
MspI CCGG 5 cut(s) 1016, 1516, 1581, 1639, 2234
MspR9I CCNGG 7 cut(s) 330, 909, 1017, 1200, 1582, 1640, 2234
MunI CAATTG 2 cut(s) 1158, 1464
Mva1269I GAATGC 1 cut(s) 1705
MvaI CCWGG 3 cut(s) 330, 909, 1200
MvnI CGCG 1 cut(s) 921
MwoI GCNNNNNNNGC 5 cut(s) 122, 1709, 1770, 2080, 2324
NciI CCSGG 4 cut(s) 1017, 1582, 1640, 2234
NdeI CATATG 2 cut(s) 676, 1838
NheI GCTAGC 1 cut(s) 1150
NlaIII CATG 7 cut(s) 250, 1142, 1219, 1449, 1658, 2174, 2191
NlaIV GGNNCC 6 cut(s) 126, 184, 1014, 1058, 1305, 1524
NmeAIII GCCGAG 2 cut(s) 1951, 2177
NsiI ATGCAT 1 cut(s) 948
OliI CACNNNNGTG 1 cut(s) 2126
PagI TCATGA 1 cut(s) 1215
PceI AGGCCT 1 cut(s) 907
PctI GAATGC 1 cut(s) 1705
PfeI GAWTC 7 cut(s) 40, 218, 362, 389, 1172, 1635, 2293
PflFI GACNNNGTC 1 cut(s) 1742
PleI GAGTC 4 cut(s) 1157, 1213, 1957, 2042
PpsI GAGTC 4 cut(s) 1157, 1213, 1957, 2042
PpuMI RGGWCCY 2 cut(s) 1523, 2356
Psp5II RGGWCCY 2 cut(s) 1523, 2356
Psp6I CCWGG 3 cut(s) 328, 907, 1198
PspGI CCWGG 3 cut(s) 328, 907, 1198
PspN4I GGNNCC 6 cut(s) 126, 184, 1014, 1058, 1305, 1524
PspPI GGNCC 3 cut(s) 1523, 2306, 2356
PspPPI RGGWCCY 2 cut(s) 1523, 2356
PsrI GAACNNNNNNTAC 2 cut(s) 1093, 1125
PstI CTGCAG 1 cut(s) 1711
PsuI RGATCY 6 cut(s) 168, 892, 1303, 1487, 1650, 1729
PsyI GACNNNGTC 1 cut(s) 1742
PvuII CAGCTG 2 cut(s) 319, 671
RsaI GTAC 4 cut(s) 655, 752, 1416, 2343
RsaNI GTAC 4 cut(s) 654, 751, 1415, 2342
RseI CAYNNNNRTG 3 cut(s) 330, 1450, 2126
SalI GTCGAC 1 cut(s) 965
SaqAI TTAA 4 cut(s) 239, 957, 980, 1590
Sau96I GGNCC 3 cut(s) 1523, 2306, 2356
SchI GAGTC 4 cut(s) 1157, 1214, 1958, 2043
ScrFI CCNGG 7 cut(s) 330, 909, 1017, 1200, 1582, 1640, 2234
SduI GDGCHC 2 cut(s) 127, 736
SfaNI GCATC 9 cut(s) 16, 644, 775, 933, 955, 1124, 1139, 1420, 1615
SfcI CTRYAG 2 cut(s) 1707, 2202
SinI GGWCC 2 cut(s) 1523, 2356
SmiMI CAYNNNNRTG 3 cut(s) 330, 1450, 2126
SseBI AGGCCT 1 cut(s) 907
SsiI CCGC 1 cut(s) 340
SspMI CTAG 5 cut(s) 60, 1146, 1151, 1377, 2090
StuI AGGCCT 1 cut(s) 907
StyD4I CCNGG 7 cut(s) 328, 907, 1015, 1198, 1580, 1638, 2232
StyI CCWWGG 1 cut(s) 1008
TaaI ACNGT 8 cut(s) 375, 503, 619, 1389, 1419, 1546, 1938, 2346
TaiI ACGT 2 cut(s) 304, 1743
TaqI TCGA 4 cut(s) 966, 1999, 2037, 2060
TatI WGTACW 2 cut(s) 653, 2341
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 7 cut(s) 40, 218, 362, 389, 1172, 1635, 2293
Tru1I TTAA 4 cut(s) 239, 957, 980, 1590
Tru9I TTAA 4 cut(s) 239, 957, 980, 1590
TscAI CASTG 6 cut(s) 378, 390, 942, 1111, 1837, 2187
TspDTI ATGAA 6 cut(s) 250, 1337, 1899, 1947, 2159, 2338
TspGWI ACGGA 1 cut(s) 1031
TspRI CASTG 6 cut(s) 378, 390, 942, 1111, 1837, 2187
Tth111I GACNNNGTC 1 cut(s) 1742
VpaK11BI GGWCC 2 cut(s) 1523, 2356
XagI CCTNNNNNAGG 1 cut(s) 2363
XapI RAATTY 6 cut(s) 49, 136, 538, 697, 976, 2056
XmiI GTMKAC 4 cut(s) 231, 480, 966, 1878
XspI CTAG 5 cut(s) 60, 1146, 1151, 1377, 2090
Zsp2I ATGCAT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.