MD07G1254000.v1.1

Domain of unknown function (DUF4535)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0017499
Physical Location & Seq
Reverse (-)
32112166 .. 32112336
171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1254000.v1.1.491

Sequence Viewer

Length: 171 bp
ATGGGGATAATCAAGAGCAGCTTCTCTTTCCTTATGGGCACGGTCGCCGGGATCTACATAGCCCAAAACTACGACGTTCCGAACATCAAGAAGCTCGGCGACACTGCCGTCTTCATCGCCAAGCAATACGAGGAGAAGTACCGGAAGCCCAAAAAGCGAGACGACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4535

Protein Analysis

57

Amino Acids

6.4

Weight (kDa)

9.3

Isoelectric Point (pI)

25.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4535 PF15054 6 - 50 3.1e-24 Domain of unknown function (DUF4535)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25225 AT4G25225
malus_domestica MD07G1254000.v1.1
prunus_persica Prupe.2G277300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0374431
rosa_roxburghii Rroxscaffold_4G00283500
rosa_rugosa Rorug01G0382800
rosa_samantha Rh1AG393800 Rh1BG358300 Rh1DG389000
rosa_wichuraiana Rw1G034150 Rw1G034230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AclWI GGATC 1 cut(s) 59
AfaI GTAC 1 cut(s) 140
AluBI AGCT 2 cut(s) 21, 94
AluI AGCT 2 cut(s) 21, 94
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 1 cut(s) 59
ApeKI GCWGC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 30
BceAI ACGGC 1 cut(s) 92
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 1 cut(s) 169
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 103
BpuMI CCSGG 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 141
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 1 cut(s) 30
Bsh1285I CGRYCG 1 cut(s) 45
BsiEI CGRYCG 1 cut(s) 45
BsiSI CCGG 2 cut(s) 48, 142
BsmAI GTCTC 1 cut(s) 153
BsmBI CGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 1 cut(s) 51
BspPI GGATC 1 cut(s) 59
BssMI GATC 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 43
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 1 cut(s) 51
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 47
BstSLI GKGCMC 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 30
BstV2I GAAGAC 1 cut(s) 103
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 100
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 102
Csp6I GTAC 1 cut(s) 139
CviJI RGCY 4 cut(s) 21, 62, 94, 148
CviKI_1 RGCY 4 cut(s) 21, 62, 94, 148
CviQI GTAC 1 cut(s) 139
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
Esp3I CGTCTC 1 cut(s) 153
FaiI YATR 2 cut(s) 35, 59
FalI AAGNNNNNCTT 1 cut(s) 37
Fnu4HI GCNGC 1 cut(s) 19
Fsp4HI GCNGC 1 cut(s) 19
FspBI CTAG 1 cut(s) 169
GluI GCNGC 1 cut(s) 19
HapII CCGG 2 cut(s) 48, 142
HpaII CCGG 2 cut(s) 48, 142
Hpy188I TCNGA 1 cut(s) 81
Hpy188III TCNNGA 2 cut(s) 13, 88
Hpy99I CGWCG 2 cut(s) 77, 167
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 1 cut(s) 75
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpySE526I ACGT 1 cut(s) 75
Kzo9I GATC 1 cut(s) 51
LpnPI CCDG 2 cut(s) 61, 155
Lsp1109I GCAGC 1 cut(s) 30
MaeI CTAG 1 cut(s) 169
MaeII ACGT 1 cut(s) 75
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 1 cut(s) 103
MflI RGATCY 1 cut(s) 51
MhlI GDGCHC 1 cut(s) 41
MnlI CCTC 1 cut(s) 124
MspI CCGG 2 cut(s) 48, 142
MspR9I CCNGG 1 cut(s) 49
MwoI GCNNNNNNNGC 1 cut(s) 154
NciI CCSGG 1 cut(s) 49
NdeII GATC 1 cut(s) 51
NmeAIII GCCGAG 1 cut(s) 75
PkrI GCNGC 1 cut(s) 20
PsuI RGATCY 1 cut(s) 51
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 49
SduI GDGCHC 1 cut(s) 41
SetI ASST 3 cut(s) 23, 78, 96
SgeI CNNG 9 cut(s) 25, 52, 60, 61, 100, 107, 133, 142, 154
SspMI CTAG 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 47
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 1 cut(s) 78
TscAI CASTG 1 cut(s) 109
TseI GCWGC 1 cut(s) 18
TspDTI ATGAA 1 cut(s) 103
TspRI CASTG 1 cut(s) 109
XspI CTAG 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.