MD07G1263600.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0022429
Physical Location & Seq
Forward (+)
33262995 .. 33263294
300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1263600.v1.1.491

Sequence Viewer

Length: 300 bp
ATGCTCTTCATTGGCCGCCTTGGCGAGCTCGCCTTAGCGGGCAAGTCCCCCGCCATAGGCTTCGCCAACATCATCGGTTACTCCATCCTCCTTGGCCTCGCCATGGGAATGGTACCCATCTGCGGCCAAGATTTTGGCGCCAAAAAACACACTATGCTCGGCCTCTCTTTGCAGAGGACGGTCCTCCTCCGACTATTTACATCCTCACCAATTTCTCTTCTCTGGCTGAACATGAAGAAAATCCTTCTCATCTGTGGCCAGGACGAAGCGATGGCTAAGCTCCCTCTGTTCTCTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

100

Amino Acids

10.65

Weight (kDa)

9.7

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 94 4.5e-21 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022429)

Species Orthologous Gene IDs
malus_domestica MD07G1263600.v1.1 MD07G1263900.v1.1
pyrus_communis pycom07g24250 pycom07g24270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 112
AccB1I GGYRCC 2 cut(s) 112, 137
AciI CCGC 4 cut(s) 16, 38, 51, 123
AcoI YGGCCR 3 cut(s) 13, 124, 256
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 3 cut(s) 56, 103, 122
AjnI CCWGG 1 cut(s) 258
AluBI AGCT 2 cut(s) 28, 280
AluI AGCT 2 cut(s) 28, 280
Alw21I GWGCWC 1 cut(s) 30
AoxI GGCC 5 cut(s) 13, 94, 124, 160, 256
Asp718I GGTACC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 198
AvaII GGWCC 1 cut(s) 181
BalI TGGCCA 1 cut(s) 258
BanI GGYRCC 2 cut(s) 112, 137
BanII GRGCYC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 30
BccI CCATC 3 cut(s) 92, 125, 265
BciT130I CCWGG 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 21
BisI GCNGC 2 cut(s) 16, 124
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 2 cut(s) 17, 125
Bme1390I CCNGG 1 cut(s) 260
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 2 cut(s) 114, 139
BmrFI CCNGG 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 34
Bpu1102I GCTNAGC 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 19, 91, 102
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 103, 122
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 3 cut(s) 19, 91, 102
BseGI GGATG 2 cut(s) 84, 200
BseLI CCNNNNNNNGG 3 cut(s) 56, 103, 122
BseRI GAGGAG 1 cut(s) 176
BshFI GGCC 5 cut(s) 15, 96, 126, 162, 258
BshNI GGYRCC 2 cut(s) 112, 137
BsiHKAI GWGCWC 1 cut(s) 30
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 3 cut(s) 56, 103, 122
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 5 cut(s) 15, 96, 126, 162, 258
Bsp1286I GDGCHC 1 cut(s) 30
Bsp1720I GCTNAGC 1 cut(s) 276
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 4 cut(s) 16, 38, 51, 123
BspANI GGCC 5 cut(s) 15, 96, 126, 162, 258
BspLI GGNNCC 2 cut(s) 114, 139
BspQI GCTCTTC 1 cut(s) 11
BspT107I GGYRCC 2 cut(s) 112, 137
BssECI CCNNGG 3 cut(s) 19, 91, 102
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 3 cut(s) 19, 91, 102
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 11, 222
BstACI GRCGYC 1 cut(s) 138
BstC8I GCNNGC 3 cut(s) 26, 30, 40
BstDEI CTNAG 2 cut(s) 34, 276
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 84, 200
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 258
BstXI CCANNNNNNTGG 2 cut(s) 109, 134
BsuRI GGCC 5 cut(s) 15, 96, 126, 162, 258
BtgI CCRYGG 1 cut(s) 102
BtgZI GCGATG 1 cut(s) 284
BtsCI GGATG 2 cut(s) 84, 200
Cac8I GCNNGC 3 cut(s) 26, 30, 40
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 181
Csp6I GTAC 1 cut(s) 113
CviAII CATG 2 cut(s) 103, 232
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 2 cut(s) 34, 276
DinI GGCGCC 1 cut(s) 139
EaeI YGGCCR 3 cut(s) 13, 124, 256
Eam1104I CTCTTC 2 cut(s) 11, 222
EarI CTCTTC 2 cut(s) 11, 222
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 19, 91, 102
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 258
EcoT14I CCWWGG 3 cut(s) 19, 91, 102
EcoT38I GRGCYC 1 cut(s) 30
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
ErhI CCWWGG 3 cut(s) 19, 91, 102
FaeI CATG 2 cut(s) 106, 235
FaiI YATR 4 cut(s) 56, 104, 155, 233
FaqI GGGAC 1 cut(s) 31
FatI CATG 2 cut(s) 102, 231
FauI CCCGC 2 cut(s) 31, 58
Fnu4HI GCNGC 2 cut(s) 16, 124
FokI GGATG 2 cut(s) 71, 187
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 2 cut(s) 16, 124
GlaI GCGC 1 cut(s) 139
GluI GCNGC 2 cut(s) 16, 124
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 5 cut(s) 15, 96, 126, 162, 258
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 2 cut(s) 106, 235
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HphI GGTGA 1 cut(s) 198
Hpy188I TCNGA 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 2 cut(s) 34, 276
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 2 cut(s) 106, 235
HspAI GCGC 1 cut(s) 138
KasI GGCGCC 1 cut(s) 137
KpnI GGTACC 1 cut(s) 116
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 3 cut(s) 208, 245, 272
MaeIII GTNAC 1 cut(s) 77
MboII GAAGA 2 cut(s) 209, 247
MhlI GDGCHC 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 258
MluCI AATT 1 cut(s) 210
MluNI TGGCCA 1 cut(s) 258
Mly113I GGCGCC 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 8 cut(s) 98, 107, 168, 173, 194, 197, 214, 294
Mox20I TGGCCA 1 cut(s) 258
MscI TGGCCA 1 cut(s) 258
MslI CAYNNNNRTG 1 cut(s) 107
Msp20I TGGCCA 1 cut(s) 258
MspR9I CCNGG 1 cut(s) 260
MvaI CCWGG 1 cut(s) 260
MwoI GCNNNNNNNGC 1 cut(s) 21
NarI GGCGCC 1 cut(s) 138
NcoI CCATGG 1 cut(s) 102
NlaIII CATG 2 cut(s) 106, 235
NlaIV GGNNCC 2 cut(s) 114, 139
NmeAIII GCCGAG 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 17, 125
PluTI GGCGCC 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 258
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 2 cut(s) 114, 139
PspPI GGNCC 1 cut(s) 181
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 11
SatI GCNGC 2 cut(s) 16, 124
Sau96I GGNCC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 1 cut(s) 30
SetI ASST 2 cut(s) 30, 282
SfoI GGCGCC 1 cut(s) 139
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 1 cut(s) 107
Sse9I AATT 1 cut(s) 210
SsiI CCGC 4 cut(s) 16, 38, 51, 123
SspDI GGCGCC 1 cut(s) 137
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 1 cut(s) 258
StyI CCWWGG 3 cut(s) 19, 91, 102
TaaI ACNGT 1 cut(s) 181
TasI AATT 1 cut(s) 210
TauI GCSGC 2 cut(s) 18, 126
TspDTI ATGAA 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.