pycom07g24250

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0022429
Physical Location & Seq
Reverse (-)
24977822 .. 24979394
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24250.2

Sequence Viewer

Length: 930 bp
ATGGATCCCAAAACCCTAACTTCCCCATTGATCTCCAAAACCATAACATCCCAACCAAAACAAGTCCCGAAGTTACAGTTCAATCCCCACAAAGAATCCCATTTTTCTTTGGCTTTAAAAGAAGCCAATTCCATAGCTGTCATAGCTTTCCCAATGATACTTACCGGCCTGTTGCTTTACTCAAGGTCAATCATCTCCATGCTCTTCTTCGGCCGCCTTGGTGAGCTCGCCGTAGCGGACGGGTCCCTTGCCGTGGGCTTCGCCAACATCACCGGTTACTACATACTCTCCGGCCTCGCCATGGGAATGGAACTCATCTGCGGCCAAGCTTTTGGCGCCAGAAAACACGCTCTGCTCGGCCCCTCTTTGCAGAGGACAGTCCTCCTCTTCCTATTTACATCCTTACCCATTTCTCTTCCCTGGCTGAACATGAAGAAAATCATCCTCATCTGCGGCCAGGACGAAGCGATCGATCAAATGGCCGAAGCTCAGTTGTACCTCCTCTGTTCTCTCCCTGATCTTCTCGCTCAATCTTTTCTCCACCCTCTGAGAATTTACCTCAGAAGTCAATCCATCACTCTGCCTCTCATTGTGTGCAACTCACGATCGATCATCCCCCACATTCCCATAAACTATTTTCTTGTTTCGCACCTCGATTTGGCAATCAAGGTTCAAGTGCCGGACGCAACTCCGAATCAAATAAACCCCACTGCTGCGGACCAAGGACTGGACACTAATTATATTGTGGCTGTGTGCAGTGGCAGGGAGGTGATCAACAAGTCCTCAACCCCGAATTCAGAGGCAACGTCTTTGAATCATAGGACTAAAACCTGTTATTTGAGACACCACTCCCTTTTCGGGTTGTTCCCGCCGATTTCATTGCAGACTCTCGTGGACACACTAAAGCTTAAGGAAGAAGGAAATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

310

Amino Acids

33.97

Weight (kDa)

8.7

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022429)

Species Orthologous Gene IDs
malus_domestica MD07G1263600.v1.1 MD07G1263900.v1.1
pyrus_communis pycom07g24250 pycom07g24270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 335
AccB7I CCANNNNNTGG 1 cut(s) 727
AciI CCGC 6 cut(s) 214, 236, 321, 453, 716, 869
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 4 cut(s) 211, 322, 454, 480
AcsI RAATTY 2 cut(s) 552, 793
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 5 cut(s) 253, 301, 658, 727, 858
AflII CTTAAG 1 cut(s) 908
AgeI ACCGGT 1 cut(s) 272
AgsI TTSAA 3 cut(s) 82, 674, 814
AjnI CCWGG 2 cut(s) 419, 456
AluBI AGCT 6 cut(s) 137, 146, 226, 329, 488, 907
AluI AGCT 6 cut(s) 137, 146, 226, 329, 488, 907
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 1 cut(s) 835
AlwI GGATC 1 cut(s) 12
AoxI GGCC 7 cut(s) 166, 211, 292, 322, 358, 454, 480
ApeKI GCWGC 1 cut(s) 713
ApoI RAATTY 2 cut(s) 552, 793
AsiGI ACCGGT 1 cut(s) 272
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 3 cut(s) 243, 359, 718
AsuHPI GGTGA 3 cut(s) 233, 262, 781
AvaII GGWCC 2 cut(s) 243, 718
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 228
BauI CACGAG 1 cut(s) 890
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 700
BccI CCATC 1 cut(s) 581
BceAI ACGGC 2 cut(s) 215, 236
BcgI CGANNNNNNTGC 2 cut(s) 862, 896
BciT130I CCWGG 2 cut(s) 421, 458
BclI TGATCA 1 cut(s) 771
BcoDI GTCTC 1 cut(s) 835
BfoI RGCGCY 1 cut(s) 339
BfrI CTTAAG 1 cut(s) 908
BisI GCNGC 4 cut(s) 214, 322, 454, 714
BlsI GCNGC 4 cut(s) 215, 323, 455, 715
Bme1390I CCNGG 2 cut(s) 421, 458
Bme18I GGWCC 2 cut(s) 243, 718
BmgT120I GGNCC 3 cut(s) 243, 359, 718
BmiI GGNNCC 5 cut(s) 6, 244, 245, 337, 361
BmrFI CCNGG 2 cut(s) 421, 458
BpuEI CTTGAG 1 cut(s) 166
Bsa29I ATCGAT 2 cut(s) 471, 608
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 5 cut(s) 217, 252, 300, 419, 721
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 5 cut(s) 253, 301, 658, 727, 858
Bse118I RCCGGY 2 cut(s) 164, 272
Bse1I ACTGG 1 cut(s) 732
Bse3DI GCAATG 1 cut(s) 878
BseBI CCWGG 2 cut(s) 421, 458
BseCI ATCGAT 2 cut(s) 471, 608
BseDI CCNNGG 5 cut(s) 217, 252, 300, 419, 721
BseGI GGATG 4 cut(s) 47, 398, 441, 612
BseLI CCNNNNNNNGG 5 cut(s) 253, 301, 658, 727, 858
BseMI GCAATG 1 cut(s) 878
BseMII CTCAG 3 cut(s) 503, 539, 574
BseNI ACTGG 1 cut(s) 732
BseRI GAGGAG 2 cut(s) 374, 491
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 1 cut(s) 700
BsgI GTGCAG 1 cut(s) 775
Bsh1285I CGRYCG 3 cut(s) 214, 471, 608
BshFI GGCC 7 cut(s) 168, 213, 294, 324, 360, 456, 482
BshNI GGYRCC 1 cut(s) 335
BshTI ACCGGT 1 cut(s) 272
BshVI ATCGAT 2 cut(s) 471, 608
BsiEI CGRYCG 3 cut(s) 214, 471, 608
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 4 cut(s) 165, 273, 291, 680
BslFI GGGAC 2 cut(s) 50, 229
BslI CCNNNNNNNGG 5 cut(s) 253, 301, 658, 727, 858
BsmAI GTCTC 1 cut(s) 835
BsmFI GGGAC 2 cut(s) 50, 229
BsnI GGCC 7 cut(s) 168, 213, 294, 324, 360, 456, 482
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 8 cut(s) 4, 30, 468, 472, 517, 605, 609, 771
Bsp19I CCATGG 1 cut(s) 300
BspACI CCGC 6 cut(s) 214, 236, 321, 453, 716, 869
BspANI GGCC 7 cut(s) 168, 213, 294, 324, 360, 456, 482
BspCNI CTCAG 3 cut(s) 502, 540, 573
BspDI ATCGAT 2 cut(s) 471, 608
BspLI GGNNCC 5 cut(s) 6, 244, 245, 337, 361
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 335
BspTI CTTAAG 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 878
BsrFI RCCGGY 2 cut(s) 164, 272
BsrI ACTGG 1 cut(s) 732
BssAI RCCGGY 2 cut(s) 164, 272
BssECI CCNNGG 5 cut(s) 217, 252, 300, 419, 721
BssMI GATC 8 cut(s) 4, 30, 468, 472, 517, 605, 609, 771
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 1 cut(s) 890
BssT1I CCWWGG 3 cut(s) 217, 300, 721
Bst2BI CACGAG 1 cut(s) 890
Bst2UI CCWGG 2 cut(s) 421, 458
Bst4CI ACNGT 2 cut(s) 78, 379
Bst6I CTCTTC 3 cut(s) 209, 392, 420
BstACI GRCGYC 1 cut(s) 336
BstAFI CTTAAG 1 cut(s) 908
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 3 cut(s) 489, 548, 560
BstDSI CCRYGG 2 cut(s) 252, 300
BstF5I GGATG 4 cut(s) 47, 398, 441, 612
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 1 cut(s) 338
BstKTI GATC 8 cut(s) 7, 33, 471, 475, 520, 608, 612, 774
BstMAI GTCTC 1 cut(s) 835
BstMBI GATC 8 cut(s) 4, 30, 468, 472, 517, 605, 609, 771
BstMCI CGRYCG 3 cut(s) 214, 471, 608
BstMWI GCNNNNNNNGC 2 cut(s) 143, 335
BstNI CCWGG 2 cut(s) 421, 458
BstSCI CCNGG 2 cut(s) 419, 456
BstV1I GCAGC 1 cut(s) 700
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 2 cut(s) 307, 332
BstYI RGATCY 1 cut(s) 4
BstZI CGGCCG 1 cut(s) 211
Bsu15I ATCGAT 2 cut(s) 471, 608
BsuRI GGCC 7 cut(s) 168, 213, 294, 324, 360, 456, 482
BsuTUI ATCGAT 2 cut(s) 471, 608
BtgI CCRYGG 2 cut(s) 252, 300
BtsCI GGATG 4 cut(s) 47, 398, 441, 612
BtsI GCAGTG 2 cut(s) 708, 763
BtsIMutI CAGTG 2 cut(s) 708, 763
Cac8I GCNNGC 1 cut(s) 228
CfoI GCGC 1 cut(s) 338
Cfr10I RCCGGY 2 cut(s) 164, 272
Cfr13I GGNCC 3 cut(s) 243, 359, 718
ClaI ATCGAT 2 cut(s) 471, 608
CseI GACGC 1 cut(s) 692
Csp6I GTAC 1 cut(s) 496
CspAI ACCGGT 1 cut(s) 272
CviAII CATG 3 cut(s) 199, 301, 430
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 3 cut(s) 489, 548, 560
DinI GGCGCC 1 cut(s) 337
DpnI GATC 8 cut(s) 6, 32, 470, 474, 519, 607, 611, 773
DpnII GATC 8 cut(s) 4, 30, 468, 472, 517, 605, 609, 771
DraI TTTAAA 1 cut(s) 117
EaeI YGGCCR 4 cut(s) 211, 322, 454, 480
EagI CGGCCG 1 cut(s) 211
Eam1104I CTCTTC 3 cut(s) 209, 392, 420
EarI CTCTTC 3 cut(s) 209, 392, 420
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 3 cut(s) 217, 300, 721
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 2 cut(s) 243, 718
Eco52I CGGCCG 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 226
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 793
EcoRII CCWGG 2 cut(s) 419, 456
EcoT14I CCWWGG 3 cut(s) 217, 300, 721
EcoT38I GRGCYC 1 cut(s) 228
EgeI GGCGCC 1 cut(s) 337
EheI GGCGCC 1 cut(s) 337
ErhI CCWWGG 3 cut(s) 217, 300, 721
FaeI CATG 3 cut(s) 202, 304, 433
FaqI GGGAC 2 cut(s) 50, 229
FatI CATG 3 cut(s) 198, 300, 429
FauI CCCGC 1 cut(s) 876
FbaI TGATCA 1 cut(s) 771
Fnu4HI GCNGC 4 cut(s) 214, 322, 454, 714
FokI GGATG 4 cut(s) 34, 385, 428, 599
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 4 cut(s) 214, 322, 454, 714
GlaI GCGC 1 cut(s) 337
GluI GCNGC 4 cut(s) 214, 322, 454, 714
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 7 cut(s) 168, 213, 294, 324, 360, 456, 482
HapII CCGG 4 cut(s) 165, 273, 291, 680
HgaI GACGC 1 cut(s) 692
HhaI GCGC 1 cut(s) 338
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 3 cut(s) 202, 304, 433
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HindIII AAGCTT 2 cut(s) 327, 905
HinfI GANTC 4 cut(s) 95, 694, 814, 886
HpaII CCGG 4 cut(s) 165, 273, 291, 680
HphI GGTGA 3 cut(s) 233, 262, 781
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 4 cut(s) 549, 563, 693, 799
Hpy188III TCNNGA 2 cut(s) 67, 603
Hpy8I GTNNAC 1 cut(s) 895
HpyAV CCTTC 1 cut(s) 911
HpyCH4III ACNGT 2 cut(s) 78, 379
HpyCH4IV ACGT 1 cut(s) 806
HpyCH4V TGCA 4 cut(s) 370, 597, 756, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 335
HpyF3I CTNAG 3 cut(s) 489, 548, 560
HpySE526I ACGT 1 cut(s) 806
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 3 cut(s) 202, 304, 433
HspAI GCGC 1 cut(s) 336
KasI GGCGCC 1 cut(s) 335
KflI GGGWCCC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 771
Kzo9I GATC 8 cut(s) 4, 30, 468, 472, 517, 605, 609, 771
LguI GCTCTTC 1 cut(s) 209
Lsp1109I GCAGC 1 cut(s) 700
MaeII ACGT 1 cut(s) 806
MaeIII GTNAC 2 cut(s) 72, 275
MalI GATC 8 cut(s) 6, 32, 470, 474, 519, 607, 611, 773
MboI GATC 8 cut(s) 4, 30, 468, 472, 517, 605, 609, 771
MboII GAAGA 7 cut(s) 196, 199, 379, 407, 445, 512, 926
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 4 cut(s) 127, 552, 736, 793
Mly113I GGCGCC 1 cut(s) 336
MlyI GAGTC 1 cut(s) 880
MseI TTAA 2 cut(s) 116, 909
MslI CAYNNNNRTG 2 cut(s) 197, 305
MspCI CTTAAG 1 cut(s) 908
MspI CCGG 4 cut(s) 165, 273, 291, 680
MspR9I CCNGG 2 cut(s) 421, 458
MvaI CCWGG 2 cut(s) 421, 458
MwoI GCNNNNNNNGC 2 cut(s) 143, 335
NarI GGCGCC 1 cut(s) 336
NcoI CCATGG 1 cut(s) 300
NdeII GATC 8 cut(s) 4, 30, 468, 472, 517, 605, 609, 771
NlaIII CATG 3 cut(s) 202, 304, 433
NlaIV GGNNCC 5 cut(s) 6, 244, 245, 337, 361
NmeAIII GCCGAG 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PfeI GAWTC 3 cut(s) 95, 694, 814
PflMI CCANNNNNTGG 1 cut(s) 727
PinAI ACCGGT 1 cut(s) 272
PkrI GCNGC 4 cut(s) 215, 323, 455, 715
Ple19I CGATCG 2 cut(s) 471, 608
PleI GAGTC 1 cut(s) 880
PluTI GGCGCC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 880
PpuMI RGGWCCY 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 419, 456
PspGI CCWGG 2 cut(s) 419, 456
PspN4I GGNNCC 5 cut(s) 6, 244, 245, 337, 361
PspPI GGNCC 3 cut(s) 243, 359, 718
PspPPI RGGWCCY 1 cut(s) 243
PsuI RGATCY 1 cut(s) 4
PvuI CGATCG 2 cut(s) 471, 608
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 2 cut(s) 197, 305
SacI GAGCTC 1 cut(s) 228
SapI GCTCTTC 1 cut(s) 209
SaqAI TTAA 2 cut(s) 116, 909
SatI GCNGC 4 cut(s) 214, 322, 454, 714
Sau3AI GATC 8 cut(s) 4, 30, 468, 472, 517, 605, 609, 771
Sau96I GGNCC 3 cut(s) 243, 359, 718
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 2 cut(s) 421, 458
SduI GDGCHC 1 cut(s) 228
SfoI GGCGCC 1 cut(s) 337
SinI GGWCC 2 cut(s) 243, 718
SmiMI CAYNNNNRTG 2 cut(s) 197, 305
SmlI CTYRAG 2 cut(s) 181, 908
SmoI CTYRAG 2 cut(s) 181, 908
Sse9I AATT 4 cut(s) 127, 552, 736, 793
SsiI CCGC 6 cut(s) 214, 236, 321, 453, 716, 869
SspDI GGCGCC 1 cut(s) 335
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 419, 456
StyI CCWWGG 3 cut(s) 217, 300, 721
TaaI ACNGT 2 cut(s) 78, 379
TaiI ACGT 1 cut(s) 809
TaqI TCGA 3 cut(s) 471, 608, 654
TasI AATT 4 cut(s) 127, 552, 736, 793
TauI GCSGC 3 cut(s) 216, 324, 456
TfiI GAWTC 3 cut(s) 95, 694, 814
Tru1I TTAA 2 cut(s) 116, 909
Tru9I TTAA 2 cut(s) 116, 909
TscAI CASTG 2 cut(s) 715, 763
TseI GCWGC 1 cut(s) 713
TspDTI ATGAA 2 cut(s) 446, 867
TspRI CASTG 2 cut(s) 715, 763
Van91I CCANNNNNTGG 1 cut(s) 727
Vha464I CTTAAG 1 cut(s) 908
VpaK11BI GGWCC 2 cut(s) 243, 718
XapI RAATTY 2 cut(s) 552, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.