MD07G1263900.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0022429
Physical Location & Seq
Reverse (-)
33264569 .. 33265021
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1263900.v1.1.491

Sequence Viewer

Length: 453 bp
ATGCTCTTCCTCGGCCGCCATGGCGAGCTCGCCTTAGCGGGCAGGTCCCTTGCCATTGGCTACGCCAACATAACCGGTTACTCCATACTCTCCGGCCTCGCCATGGGAATGCAACTCATCTGCGGCCAAGCTTTTGGCGCCAGAAAACACACTCTGCTCGGCCTCTCTTTGCAGAGGACAGTCCTCCTCTTACTATTTACATCCTTACCCATTTCTCTTCTCTGGCTGAACATGAAGAAAATCATCCTCATCTGCGGCCAGTATGAAACGATCGCTGCCGAAGCTCAGTTGTATTCTCTCCCTGATCTTCTCACTCAATCTTTTCTCCACCCTTTGAGAATTTACCTCAGAAGTCAATCCATCACTCTGCCTATCCCCTACATTCCCATAAACAATTTTCTCGTTTCGCACCTTGATTTGGCAATCAAAGGTGTTGTTTTTAGTGGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

151

Amino Acids

16.44

Weight (kDa)

9.51

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 133 6e-23 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022429)

Species Orthologous Gene IDs
malus_domestica MD07G1263600.v1.1 MD07G1263900.v1.1
pyrus_communis pycom07g24250 pycom07g24270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 33
AccB1I GGYRCC 1 cut(s) 137
AciI CCGC 4 cut(s) 16, 38, 123, 255
AcoI YGGCCR 3 cut(s) 13, 124, 256
AcsI RAATTY 1 cut(s) 339
AcyI GRCGYC 1 cut(s) 138
AfiI CCNNNNNNNGG 2 cut(s) 103, 418
AgeI ACCGGT 1 cut(s) 74
AluBI AGCT 3 cut(s) 28, 131, 284
AluI AGCT 3 cut(s) 28, 131, 284
Alw21I GWGCWC 1 cut(s) 30
AoxI GGCC 5 cut(s) 13, 94, 124, 160, 256
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 339
AsiGI ACCGGT 1 cut(s) 74
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 45
AvaII GGWCC 1 cut(s) 45
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 262
BccI CCATC 1 cut(s) 368
BfoI RGCGCY 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 33
BglI GCCNNNNNGGC 1 cut(s) 21
BisI GCNGC 4 cut(s) 16, 124, 256, 276
BlsI GCNGC 4 cut(s) 17, 125, 257, 277
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 2 cut(s) 47, 139
Bpu10I CCTNAGC 1 cut(s) 34
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 10, 19, 102
BsaWI WCCGGW 1 cut(s) 74
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 418
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 1 cut(s) 259
BseDI CCNNGG 3 cut(s) 10, 19, 102
BseGI GGATG 2 cut(s) 200, 243
BseLI CCNNNNNNNGG 2 cut(s) 103, 418
BseMII CTCAG 2 cut(s) 299, 361
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 1 cut(s) 176
BseX3I CGGCCG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 262
Bsh1285I CGRYCG 2 cut(s) 16, 273
BshFI GGCC 5 cut(s) 15, 96, 126, 162, 258
BshNI GGYRCC 1 cut(s) 137
BshTI ACCGGT 1 cut(s) 74
BsiEI CGRYCG 2 cut(s) 16, 273
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 2 cut(s) 75, 93
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 2 cut(s) 103, 418
BsmFI GGGAC 1 cut(s) 31
BsmI GAATGC 1 cut(s) 114
BsnI GGCC 5 cut(s) 15, 96, 126, 162, 258
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 2 cut(s) 270, 304
Bsp19I CCATGG 2 cut(s) 19, 102
BspACI CCGC 4 cut(s) 16, 38, 123, 255
BspANI GGCC 5 cut(s) 15, 96, 126, 162, 258
BspCNI CTCAG 2 cut(s) 298, 360
BspLI GGNNCC 2 cut(s) 47, 139
BspMI ACCTGC 1 cut(s) 33
BspQI GCTCTTC 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 137
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 10, 19, 102
BssMI GATC 2 cut(s) 270, 304
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 19, 102
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 11, 222
BstACI GRCGYC 1 cut(s) 138
BstC8I GCNNGC 3 cut(s) 26, 30, 40
BstDEI CTNAG 3 cut(s) 34, 285, 347
BstDSI CCRYGG 2 cut(s) 19, 102
BstF5I GGATG 2 cut(s) 200, 243
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 2 cut(s) 273, 307
BstMBI GATC 2 cut(s) 270, 304
BstMCI CGRYCG 2 cut(s) 16, 273
BstMWI GCNNNNNNNGC 3 cut(s) 21, 137, 281
BstV1I GCAGC 1 cut(s) 262
BstXI CCANNNNNNTGG 1 cut(s) 134
BstZI CGGCCG 1 cut(s) 13
BsuRI GGCC 5 cut(s) 15, 96, 126, 162, 258
BtgI CCRYGG 2 cut(s) 19, 102
BtsCI GGATG 2 cut(s) 200, 243
BveI ACCTGC 1 cut(s) 33
Cac8I GCNNGC 3 cut(s) 26, 30, 40
CfoI GCGC 1 cut(s) 140
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 45
CspAI ACCGGT 1 cut(s) 74
CviAII CATG 3 cut(s) 20, 103, 232
DdeI CTNAG 3 cut(s) 34, 285, 347
DinI GGCGCC 1 cut(s) 139
DpnI GATC 2 cut(s) 272, 306
DpnII GATC 2 cut(s) 270, 304
EaeI YGGCCR 3 cut(s) 13, 124, 256
EagI CGGCCG 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 11, 222
EarI CTCTTC 2 cut(s) 11, 222
Ecl136II GAGCTC 1 cut(s) 28
EclXI CGGCCG 1 cut(s) 13
Eco130I CCWWGG 2 cut(s) 19, 102
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 45
Eco52I CGGCCG 1 cut(s) 13
Eco53kI GAGCTC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 45
EcoT14I CCWWGG 2 cut(s) 19, 102
EcoT38I GRGCYC 1 cut(s) 30
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
ErhI CCWWGG 2 cut(s) 19, 102
FaeI CATG 3 cut(s) 23, 106, 235
FaiI YATR 7 cut(s) 21, 71, 86, 104, 233, 264, 389
FaqI GGGAC 1 cut(s) 31
FatI CATG 3 cut(s) 19, 102, 231
FauI CCCGC 1 cut(s) 31
Fnu4HI GCNGC 4 cut(s) 16, 124, 256, 276
FokI GGATG 2 cut(s) 187, 230
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 4 cut(s) 16, 124, 256, 276
GlaI GCGC 1 cut(s) 139
GluI GCNGC 4 cut(s) 16, 124, 256, 276
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 5 cut(s) 15, 96, 126, 162, 258
HapII CCGG 2 cut(s) 75, 93
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 3 cut(s) 23, 106, 235
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 129
HpaII CCGG 2 cut(s) 75, 93
Hpy188I TCNGA 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 112, 172
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 137, 281
HpyF3I CTNAG 3 cut(s) 34, 285, 347
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 3 cut(s) 23, 106, 235
HspAI GCGC 1 cut(s) 138
KasI GGCGCC 1 cut(s) 137
Kzo9I GATC 2 cut(s) 270, 304
LguI GCTCTTC 1 cut(s) 11
LpnPI CCDG 7 cut(s) 28, 88, 106, 154, 208, 272, 315
Lsp1109I GCAGC 1 cut(s) 262
MaeIII GTNAC 1 cut(s) 77
MalI GATC 2 cut(s) 272, 306
MboI GATC 2 cut(s) 270, 304
MboII GAAGA 3 cut(s) 209, 247, 299
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 2 cut(s) 339, 394
Mly113I GGCGCC 1 cut(s) 138
MnlI CCTC 8 cut(s) 20, 107, 168, 173, 194, 197, 257, 356
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 2 cut(s) 75, 93
Mva1269I GAATGC 1 cut(s) 114
MwoI GCNNNNNNNGC 3 cut(s) 21, 137, 281
NarI GGCGCC 1 cut(s) 138
NcoI CCATGG 2 cut(s) 19, 102
NdeII GATC 2 cut(s) 270, 304
NlaIII CATG 3 cut(s) 23, 106, 235
NlaIV GGNNCC 2 cut(s) 47, 139
NmeAIII GCCGAG 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 11
PctI GAATGC 1 cut(s) 114
PinAI ACCGGT 1 cut(s) 74
PkrI GCNGC 4 cut(s) 17, 125, 257, 277
Ple19I CGATCG 1 cut(s) 273
PluTI GGCGCC 1 cut(s) 141
PpuMI RGGWCCY 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 45
PspN4I GGNNCC 2 cut(s) 47, 139
PspPI GGNCC 1 cut(s) 45
PspPPI RGGWCCY 1 cut(s) 45
PvuI CGATCG 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 11
SatI GCNGC 4 cut(s) 16, 124, 256, 276
Sau3AI GATC 2 cut(s) 270, 304
Sau96I GGNCC 1 cut(s) 45
SduI GDGCHC 1 cut(s) 30
SetI ASST 7 cut(s) 30, 47, 133, 286, 348, 414, 433
SfoI GGCGCC 1 cut(s) 139
SinI GGWCC 1 cut(s) 45
SmiMI CAYNNNNRTG 1 cut(s) 107
Sse9I AATT 2 cut(s) 339, 394
SsiI CCGC 4 cut(s) 16, 38, 123, 255
SspDI GGCGCC 1 cut(s) 137
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 2 cut(s) 19, 102
TaaI ACNGT 1 cut(s) 181
TasI AATT 2 cut(s) 339, 394
TauI GCSGC 3 cut(s) 18, 126, 258
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 2 cut(s) 248, 279
VpaK11BI GGWCC 1 cut(s) 45
XapI RAATTY 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.