MD07G1310600.v1.1

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0021897
Physical Location & Seq
Forward (+)
36531394 .. 36532190
797 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1310600.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGTTGCTGCCGCCGGTGAGGGAGCCGCAGGTACAGTTATAACAACGCGGCCGAGGAGGAGCGTGGTTTTCACGTACGGCACCCTAAAGCGGGGGTTTGGGAACCACGCCTTAATGGAGGAGCTGACGAGAAGCGGAGATGCCGTATTCTTGGGCAGCTACCGAACCCAAGAAAAGTATCCGCTGGTGTGCGGGCCCTACCGGGTGCCATTTCTCCTCAACCTTCCAGGATCGGGTGAGCGGGTAACGGGGGAGTTGTACGGCGTGTCGAGTGGCGGGCTGGCTCGCATGGATGAGTTGGAGGGCACGAGTCGCGGTCACTACGAGAGGCTGCCCATAAAGCTGGTGGTCCTGGACGAGGGGGAGACGGAGGAAGCGGAGGCGTATTTTGCGCACAGCAGCTACGCAAGGGAGCTGTGGATGAAGAACGGGAGGAGGGGTTTGTGCGCGTATTCGGAGAAAGAAGCGCGCGGGTACGTGAAGCGCAATGAAAGGCCGCAGCATCTCACCTTTCTCGACCACATTCATCTGTTTGTGTCCTCCGCCTCCGACGAACCATCAGATTCATCAGATCCATTGACTTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009628 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

197

Amino Acids

21.71

Weight (kDa)

6.09

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 23 - 133 3.1e-21 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02910
malus_domestica MD07G1310600.v1.1
prunus_persica Prupe.2G328600_v2.0.a1
pyrus_communis pycom07g28280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
Acc16I TGCGCA 1 cut(s) 393
Acc36I ACCTGC 1 cut(s) 20
AccB1I GGYRCC 2 cut(s) 80, 205
AccBSI CCGCTC 1 cut(s) 241
AccII CGCG 5 cut(s) 49, 315, 449, 469, 471
AclWI GGATC 2 cut(s) 238, 566
AcoI YGGCCR 1 cut(s) 50
AfaI GTAC 4 cut(s) 34, 77, 260, 476
AfiI CCNNNNNNNGG 4 cut(s) 90, 91, 233, 358
AgsI TTSAA 1 cut(s) 586
AjnI CCWGG 2 cut(s) 226, 351
AluBI AGCT 5 cut(s) 124, 159, 343, 402, 415
AluI AGCT 5 cut(s) 124, 159, 343, 402, 415
Alw26I GTCTC 1 cut(s) 359
AlwI GGATC 2 cut(s) 238, 566
AoxI GGCC 3 cut(s) 50, 194, 494
ApaI GGGCCC 1 cut(s) 198
ApeKI GCWGC 5 cut(s) 8, 156, 331, 399, 499
AspLEI GCGC 5 cut(s) 394, 449, 469, 471, 486
AspS9I GGNCC 3 cut(s) 194, 195, 349
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 3 cut(s) 29, 248, 499
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 2 cut(s) 198, 308
BanI GGYRCC 2 cut(s) 80, 205
BanII GRGCYC 1 cut(s) 198
BauI CACGAG 1 cut(s) 307
BbvI GCAGC 4 cut(s) 168, 318, 411, 511
BccI CCATC 1 cut(s) 565
BceAI ACGGC 3 cut(s) 94, 128, 277
BciT130I CCWGG 2 cut(s) 228, 353
BciVI GTATCC 1 cut(s) 189
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 20
BfuI GTATCC 1 cut(s) 189
BisI GCNGC 9 cut(s) 9, 12, 27, 50, 157, 332, 400, 497, 500
BlsI GCNGC 9 cut(s) 10, 13, 28, 51, 158, 333, 401, 498, 501
Bme1390I CCNGG 3 cut(s) 203, 228, 353
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 3 cut(s) 194, 195, 349
BmiI GGNNCC 5 cut(s) 25, 82, 104, 196, 207
BmrFI CCNGG 3 cut(s) 203, 228, 353
BmsI GCATC 2 cut(s) 130, 511
BpuMI CCSGG 1 cut(s) 203
BsaAI YACGTR 2 cut(s) 75, 478
BsaJI CCNNGG 1 cut(s) 53
BsaXI ACNNNNNCTCC 4 cut(s) 129, 159, 424, 454
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 91, 233, 358
Bse118I RCCGGY 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 2 cut(s) 228, 353
BseDI CCNNGG 1 cut(s) 53
BseGI GGATG 2 cut(s) 298, 426
BseLI CCNNNNNNNGG 4 cut(s) 90, 91, 233, 358
BseMI GCAATG 1 cut(s) 493
BsePI GCGCGC 1 cut(s) 467
BseRI GAGGAG 5 cut(s) 70, 73, 134, 206, 448
BseSI GKGCMC 2 cut(s) 198, 308
BseX3I CGGCCG 1 cut(s) 50
BseXI GCAGC 4 cut(s) 168, 318, 411, 511
Bsh1236I CGCG 5 cut(s) 49, 315, 449, 469, 471
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 3 cut(s) 52, 196, 496
BshNI GGYRCC 2 cut(s) 80, 205
BsiEI CGRYCG 1 cut(s) 53
BsiSI CCGG 2 cut(s) 15, 202
BsiWI CGTACG 1 cut(s) 75
BslI CCNNNNNNNGG 4 cut(s) 90, 91, 233, 358
BsmAI GTCTC 1 cut(s) 359
BsmBI CGTCTC 1 cut(s) 359
BsnI GGCC 3 cut(s) 52, 196, 496
Bsp120I GGGCCC 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 198, 308
Bsp143I GATC 2 cut(s) 230, 571
BspANI GGCC 3 cut(s) 52, 196, 496
BspFNI CGCG 5 cut(s) 49, 315, 449, 469, 471
BspLI GGNNCC 5 cut(s) 25, 82, 104, 196, 207
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 2 cut(s) 238, 566
BspT107I GGYRCC 2 cut(s) 80, 205
BsrBI CCGCTC 1 cut(s) 241
BsrDI GCAATG 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 53
BssHII GCGCGC 1 cut(s) 467
BssMI GATC 2 cut(s) 230, 571
BssSI CACGAG 1 cut(s) 307
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 228, 353
Bst4CI ACNGT 1 cut(s) 37
BstBAI YACGTR 2 cut(s) 75, 478
BstC8I GCNNGC 5 cut(s) 194, 278, 282, 286, 469
BstENI CCTNNNNNAGG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 298, 426
BstFNI CGCG 5 cut(s) 49, 315, 449, 469, 471
BstHHI GCGC 5 cut(s) 394, 449, 469, 471, 486
BstKTI GATC 2 cut(s) 233, 574
BstMAI GTCTC 1 cut(s) 359
BstMBI GATC 2 cut(s) 230, 571
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 3 cut(s) 312, 340, 389
BstNI CCWGG 2 cut(s) 228, 353
BstSCI CCNGG 3 cut(s) 201, 226, 351
BstSLI GKGCMC 2 cut(s) 198, 308
BstUI CGCG 5 cut(s) 49, 315, 449, 469, 471
BstV1I GCAGC 4 cut(s) 168, 318, 411, 511
BstX2I RGATCY 1 cut(s) 571
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 1 cut(s) 571
BstZI CGGCCG 1 cut(s) 50
BsuI GTATCC 1 cut(s) 189
BsuRI GGCC 3 cut(s) 52, 196, 496
BtsCI GGATG 2 cut(s) 298, 426
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 5 cut(s) 194, 278, 282, 286, 469
CfoI GCGC 5 cut(s) 394, 449, 469, 471, 486
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 3 cut(s) 194, 195, 349
Csp6I GTAC 4 cut(s) 33, 76, 259, 475
CviAII CATG 1 cut(s) 289
CviQI GTAC 4 cut(s) 33, 76, 259, 475
DpnI GATC 2 cut(s) 232, 573
DpnII GATC 2 cut(s) 230, 571
EaeI YGGCCR 1 cut(s) 50
EagI CGGCCG 1 cut(s) 50
EciI GGCGGA 1 cut(s) 532
EclXI CGGCCG 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 349
Eco52I CGGCCG 1 cut(s) 50
EcoNI CCTNNNNNAGG 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 195
EcoRII CCWGG 2 cut(s) 226, 351
EcoT38I GRGCYC 1 cut(s) 198
Esp3I CGTCTC 1 cut(s) 359
FaeI CATG 1 cut(s) 292
FaiI YATR 3 cut(s) 41, 290, 338
FatI CATG 1 cut(s) 288
FauI CCCGC 5 cut(s) 84, 185, 234, 269, 464
Fnu4HI GCNGC 9 cut(s) 9, 12, 27, 50, 157, 332, 400, 497, 500
FokI GGATG 2 cut(s) 305, 433
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 9 cut(s) 9, 12, 27, 50, 157, 332, 400, 497, 500
FspI TGCGCA 1 cut(s) 393
GlaI GCGC 5 cut(s) 393, 448, 468, 470, 485
GluI GCNGC 9 cut(s) 9, 12, 27, 50, 157, 332, 400, 497, 500
HaeIII GGCC 3 cut(s) 52, 196, 496
HapII CCGG 2 cut(s) 15, 202
HhaI GCGC 5 cut(s) 394, 449, 469, 471, 486
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 5 cut(s) 392, 447, 467, 469, 484
HinP1I GCGC 5 cut(s) 392, 447, 467, 469, 484
HinfI GANTC 2 cut(s) 310, 563
HpaII CCGG 2 cut(s) 15, 202
HphI GGTGA 3 cut(s) 29, 248, 499
Hpy188I TCNGA 4 cut(s) 457, 550, 562, 571
Hpy188III TCNNGA 1 cut(s) 515
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 2 cut(s) 74, 477
HpyF10VI GCNNNNNNNGC 3 cut(s) 312, 340, 389
HpySE526I ACGT 2 cut(s) 74, 477
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 5 cut(s) 392, 447, 467, 469, 484
Kzo9I GATC 2 cut(s) 230, 571
LmnI GCTCC 4 cut(s) 23, 60, 121, 412
Lsp1109I GCAGC 4 cut(s) 168, 318, 411, 511
LweI GCATC 2 cut(s) 130, 511
MaeII ACGT 2 cut(s) 74, 477
MaeIII GTNAC 2 cut(s) 244, 317
MalI GATC 2 cut(s) 232, 573
MbiI CCGCTC 1 cut(s) 241
MboI GATC 2 cut(s) 230, 571
MboII GAAGA 1 cut(s) 436
MflI RGATCY 1 cut(s) 571
MhlI GDGCHC 2 cut(s) 198, 308
MluCI AATT 1 cut(s) 586
MlyI GAGTC 1 cut(s) 319
MmeI TCCRAC 2 cut(s) 279, 573
MseI TTAA 2 cut(s) 113, 589
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 2 cut(s) 15, 202
MspR9I CCNGG 3 cut(s) 203, 228, 353
MvaI CCWGG 2 cut(s) 228, 353
MvnI CGCG 5 cut(s) 49, 315, 449, 469, 471
MwoI GCNNNNNNNGC 3 cut(s) 312, 340, 389
NciI CCSGG 1 cut(s) 203
NdeII GATC 2 cut(s) 230, 571
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 5 cut(s) 25, 82, 104, 196, 207
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 317
NsbI TGCGCA 1 cut(s) 393
PauI GCGCGC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 563
Pfl23II CGTACG 1 cut(s) 75
PfoI TCCNGGA 2 cut(s) 226, 351
PkrI GCNGC 9 cut(s) 10, 13, 28, 51, 158, 333, 401, 498, 501
PleI GAGTC 1 cut(s) 318
PpsI GAGTC 1 cut(s) 318
Ppu21I YACGTR 2 cut(s) 75, 478
PsiI TTATAA 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 226, 351
PspGI CCWGG 2 cut(s) 226, 351
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 5 cut(s) 25, 82, 104, 196, 207
PspOMI GGGCCC 1 cut(s) 194
PspPI GGNCC 3 cut(s) 194, 195, 349
PsuI RGATCY 1 cut(s) 571
PteI GCGCGC 1 cut(s) 467
RsaI GTAC 4 cut(s) 34, 77, 260, 476
RsaNI GTAC 4 cut(s) 33, 76, 259, 475
SaqAI TTAA 2 cut(s) 113, 589
SatI GCNGC 9 cut(s) 9, 12, 27, 50, 157, 332, 400, 497, 500
Sau3AI GATC 2 cut(s) 230, 571
Sau96I GGNCC 3 cut(s) 194, 195, 349
SchI GAGTC 1 cut(s) 319
ScrFI CCNGG 3 cut(s) 203, 228, 353
SduI GDGCHC 2 cut(s) 198, 308
SfaNI GCATC 2 cut(s) 130, 511
SgrAI CRCCGGYG 1 cut(s) 14
SinI GGWCC 1 cut(s) 349
Sse9I AATT 1 cut(s) 586
StyD4I CCNGG 3 cut(s) 201, 226, 351
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 2 cut(s) 77, 480
TaqI TCGA 2 cut(s) 269, 516
TasI AATT 1 cut(s) 586
TauI GCSGC 4 cut(s) 14, 29, 52, 499
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 2 cut(s) 113, 589
Tru9I TTAA 2 cut(s) 113, 589
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 5 cut(s) 8, 156, 331, 399, 499
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 4 cut(s) 437, 504, 515, 555
TspGWI ACGGA 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 349
XagI CCTNNNNNAGG 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.