pycom07g28280

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0021897
Physical Location & Seq
Forward (+)
27639319 .. 27639912
594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g28280.1

Sequence Viewer

Length: 594 bp
ATGGTTGCTGCCGCCGATGAGGGAACCACAGGTACAGGTATAACAACGCCGCCGAGGAGGAGCGTGGTTTTCACGTACGGCACCCTAAAGCGGGGGTTTGGGAACCACGCCTTAATGGAGGAGCTGATGAGAAGCGGAGACGCCGTATTCTTGGGCAGCTACCGAACCCAAGAAAACTTTCCGCTAGTGTGCGGGCCCTACCAGGTGCCATTTCTCCTCAACCTGCCAGGATCGGGTGAACGGGTAACGGGGGAGTTGTACGGCGTGTCGAGTGGCGGGCTGGCTCGCATGGATGAGTTGGAGGGCACGAGTCGCGGTCACTACGAGAGGCTGCCCATAAAGCTGGTGGTCCTGGAGGAGGGGGAGGCGGAGGAAGCGGAGGCGTATTTTGCGCACAGCAGCTACGCAAGGGAGCTGTGGATGAAGAACGGGAGGAGGGGTTTGAGCGCGTATTCGGAGAAAGAGGCGCGTGGGTACGTGAAGCGCAATGAAAGGCCGCAGCATCTCACCTTTCTCGACCACATTCAACTGTTTGTGTCCGCCGCCTCCGACGAACCATCAGATTCATCGGATCCATTGACTTGCAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009628 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

198

Amino Acids

21.73

Weight (kDa)

5.22

Isoelectric Point (pI)

39.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 23 - 133 1.6e-20 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02910
malus_domestica MD07G1310600.v1.1
prunus_persica Prupe.2G328600_v2.0.a1
pyrus_communis pycom07g28280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 393
Acc36I ACCTGC 1 cut(s) 231
AccB1I GGYRCC 2 cut(s) 80, 205
AccII CGCG 3 cut(s) 315, 449, 469
AclWI GGATC 3 cut(s) 238, 566, 579
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 4 cut(s) 34, 77, 260, 476
AfiI CCNNNNNNNGG 4 cut(s) 90, 91, 233, 358
AgsI TTSAA 1 cut(s) 527
AjnI CCWGG 3 cut(s) 201, 226, 351
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 5 cut(s) 124, 159, 343, 402, 415
AluI AGCT 5 cut(s) 124, 159, 343, 402, 415
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 3 cut(s) 238, 566, 579
AoxI GGCC 2 cut(s) 194, 494
ApaI GGGCCC 1 cut(s) 198
ApeKI GCWGC 5 cut(s) 8, 156, 331, 399, 499
Asp700I GAANNNNTTC 1 cut(s) 177
AspLEI GCGC 4 cut(s) 394, 449, 469, 486
AspS9I GGNCC 3 cut(s) 194, 195, 349
AsuHPI GGTGA 2 cut(s) 248, 499
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 2 cut(s) 198, 308
BamHI GGATCC 1 cut(s) 571
BanI GGYRCC 2 cut(s) 80, 205
BanII GRGCYC 1 cut(s) 198
BauI CACGAG 1 cut(s) 307
BbvI GCAGC 4 cut(s) 168, 318, 411, 511
BccI CCATC 1 cut(s) 565
BceAI ACGGC 3 cut(s) 94, 128, 277
BciT130I CCWGG 3 cut(s) 203, 228, 353
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 231
BisI GCNGC 9 cut(s) 9, 12, 50, 157, 332, 400, 497, 500, 543
BlsI GCNGC 9 cut(s) 10, 13, 51, 158, 333, 401, 498, 501, 544
Bme1390I CCNGG 3 cut(s) 203, 228, 353
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 3 cut(s) 194, 195, 349
BmiI GGNNCC 6 cut(s) 25, 82, 104, 196, 207, 573
BmrFI CCNGG 3 cut(s) 203, 228, 353
BmsI GCATC 1 cut(s) 511
BpmI CTGGAG 1 cut(s) 374
BsaAI YACGTR 2 cut(s) 75, 478
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 53
BsaXI ACNNNNNCTCC 4 cut(s) 129, 159, 424, 454
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 91, 233, 358
Bse3DI GCAATG 2 cut(s) 493, 592
BseBI CCWGG 3 cut(s) 203, 228, 353
BseDI CCNNGG 1 cut(s) 53
BseGI GGATG 2 cut(s) 298, 426
BseLI CCNNNNNNNGG 4 cut(s) 90, 91, 233, 358
BseMI GCAATG 2 cut(s) 493, 592
BseRI GAGGAG 6 cut(s) 70, 73, 134, 206, 371, 448
BseSI GKGCMC 2 cut(s) 198, 308
BseXI GCAGC 4 cut(s) 168, 318, 411, 511
Bsh1236I CGCG 3 cut(s) 315, 449, 469
BshFI GGCC 2 cut(s) 196, 496
BshNI GGYRCC 2 cut(s) 80, 205
BsiWI CGTACG 1 cut(s) 75
BslI CCNNNNNNNGG 4 cut(s) 90, 91, 233, 358
BsmAI GTCTC 1 cut(s) 132
BsmBI CGTCTC 1 cut(s) 132
BsnI GGCC 2 cut(s) 196, 496
Bsp120I GGGCCC 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 198, 308
Bsp143I GATC 2 cut(s) 230, 571
BspANI GGCC 2 cut(s) 196, 496
BspFNI CGCG 3 cut(s) 315, 449, 469
BspLI GGNNCC 6 cut(s) 25, 82, 104, 196, 207, 573
BspMI ACCTGC 1 cut(s) 231
BspPI GGATC 3 cut(s) 238, 566, 579
BspT107I GGYRCC 2 cut(s) 80, 205
BsrDI GCAATG 2 cut(s) 493, 592
BssECI CCNNGG 1 cut(s) 53
BssMI GATC 2 cut(s) 230, 571
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 307
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 203, 228, 353
Bst4CI ACNGT 1 cut(s) 531
BstACI GRCGYC 1 cut(s) 141
BstBAI YACGTR 2 cut(s) 75, 478
BstC8I GCNNGC 4 cut(s) 194, 278, 282, 286
BstENI CCTNNNNNAGG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 298, 426
BstFNI CGCG 3 cut(s) 315, 449, 469
BstHHI GCGC 4 cut(s) 394, 449, 469, 486
BstKTI GATC 2 cut(s) 233, 574
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 230, 571
BstMWI GCNNNNNNNGC 4 cut(s) 312, 340, 374, 389
BstNI CCWGG 3 cut(s) 203, 228, 353
BstSCI CCNGG 3 cut(s) 201, 226, 351
BstSLI GKGCMC 2 cut(s) 198, 308
BstUI CGCG 3 cut(s) 315, 449, 469
BstV1I GCAGC 4 cut(s) 168, 318, 411, 511
BstX2I RGATCY 1 cut(s) 571
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 1 cut(s) 571
BsuRI GGCC 2 cut(s) 196, 496
BtsCI GGATG 2 cut(s) 298, 426
BveI ACCTGC 1 cut(s) 231
Cac8I GCNNGC 4 cut(s) 194, 278, 282, 286
CfoI GCGC 4 cut(s) 394, 449, 469, 486
Cfr13I GGNCC 3 cut(s) 194, 195, 349
CseI GACGC 1 cut(s) 149
CsiI ACCWGGT 1 cut(s) 201
Csp6I GTAC 4 cut(s) 33, 76, 259, 475
CviAII CATG 1 cut(s) 289
CviQI GTAC 4 cut(s) 33, 76, 259, 475
DpnI GATC 2 cut(s) 232, 573
DpnII GATC 2 cut(s) 230, 571
EciI GGCGGA 2 cut(s) 383, 529
Eco24I GRGCYC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 195
EcoRII CCWGG 3 cut(s) 201, 226, 351
EcoT38I GRGCYC 1 cut(s) 198
Esp3I CGTCTC 1 cut(s) 132
FaeI CATG 1 cut(s) 292
FaiI YATR 3 cut(s) 41, 290, 338
FatI CATG 1 cut(s) 288
FauI CCCGC 3 cut(s) 84, 185, 269
Fnu4HI GCNGC 9 cut(s) 9, 12, 50, 157, 332, 400, 497, 500, 543
FokI GGATG 2 cut(s) 305, 433
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 9 cut(s) 9, 12, 50, 157, 332, 400, 497, 500, 543
FspBI CTAG 1 cut(s) 185
FspI TGCGCA 1 cut(s) 393
GlaI GCGC 4 cut(s) 393, 448, 468, 485
GluI GCNGC 9 cut(s) 9, 12, 50, 157, 332, 400, 497, 500, 543
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 2 cut(s) 196, 496
HgaI GACGC 1 cut(s) 149
HhaI GCGC 4 cut(s) 394, 449, 469, 486
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 4 cut(s) 392, 447, 467, 484
HinP1I GCGC 4 cut(s) 392, 447, 467, 484
HinfI GANTC 2 cut(s) 310, 563
HphI GGTGA 2 cut(s) 248, 499
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 4 cut(s) 457, 550, 562, 571
Hpy188III TCNNGA 1 cut(s) 515
Hpy8I GTNNAC 1 cut(s) 239
Hpy99I CGWCG 1 cut(s) 554
HpyCH4III ACNGT 1 cut(s) 531
HpyCH4IV ACGT 2 cut(s) 74, 477
HpyCH4V TGCA 1 cut(s) 585
HpyF10VI GCNNNNNNNGC 4 cut(s) 312, 340, 374, 389
HpySE526I ACGT 2 cut(s) 74, 477
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 4 cut(s) 392, 447, 467, 484
Kzo9I GATC 2 cut(s) 230, 571
LmnI GCTCC 3 cut(s) 60, 121, 412
Lsp1109I GCAGC 4 cut(s) 168, 318, 411, 511
LweI GCATC 1 cut(s) 511
MabI ACCWGGT 1 cut(s) 201
MaeI CTAG 1 cut(s) 185
MaeII ACGT 2 cut(s) 74, 477
MaeIII GTNAC 2 cut(s) 244, 317
MalI GATC 2 cut(s) 232, 573
MboI GATC 2 cut(s) 230, 571
MboII GAAGA 1 cut(s) 436
MflI RGATCY 1 cut(s) 571
MhlI GDGCHC 2 cut(s) 198, 308
MlyI GAGTC 1 cut(s) 319
MmeI TCCRAC 2 cut(s) 279, 573
MroXI GAANNNNTTC 1 cut(s) 177
MseI TTAA 1 cut(s) 113
MspR9I CCNGG 3 cut(s) 203, 228, 353
MvaI CCWGG 3 cut(s) 203, 228, 353
MvnI CGCG 3 cut(s) 315, 449, 469
MwoI GCNNNNNNNGC 4 cut(s) 312, 340, 374, 389
NdeII GATC 2 cut(s) 230, 571
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 6 cut(s) 25, 82, 104, 196, 207, 573
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 317
NsbI TGCGCA 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 1 cut(s) 563
Pfl23II CGTACG 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 351
PkrI GCNGC 9 cut(s) 10, 13, 51, 158, 333, 401, 498, 501, 544
PleI GAGTC 1 cut(s) 318
PpsI GAGTC 1 cut(s) 318
Ppu21I YACGTR 2 cut(s) 75, 478
Psp6I CCWGG 3 cut(s) 201, 226, 351
PspGI CCWGG 3 cut(s) 201, 226, 351
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 6 cut(s) 25, 82, 104, 196, 207, 573
PspOMI GGGCCC 1 cut(s) 194
PspPI GGNCC 3 cut(s) 194, 195, 349
PsrI GAACNNNNNNTAC 2 cut(s) 16, 48
PsuI RGATCY 1 cut(s) 571
RsaI GTAC 4 cut(s) 34, 77, 260, 476
RsaNI GTAC 4 cut(s) 33, 76, 259, 475
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 9 cut(s) 9, 12, 50, 157, 332, 400, 497, 500, 543
Sau3AI GATC 2 cut(s) 230, 571
Sau96I GGNCC 3 cut(s) 194, 195, 349
SchI GAGTC 1 cut(s) 319
ScrFI CCNGG 3 cut(s) 203, 228, 353
SduI GDGCHC 2 cut(s) 198, 308
SexAI ACCWGGT 1 cut(s) 201
SfaNI GCATC 1 cut(s) 511
SinI GGWCC 1 cut(s) 349
SspMI CTAG 1 cut(s) 185
StyD4I CCNGG 3 cut(s) 201, 226, 351
TaaI ACNGT 1 cut(s) 531
TaiI ACGT 2 cut(s) 77, 480
TaqI TCGA 2 cut(s) 269, 516
TauI GCSGC 4 cut(s) 14, 52, 499, 545
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 5 cut(s) 8, 156, 331, 399, 499
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 3 cut(s) 437, 504, 555
VpaK11BI GGWCC 1 cut(s) 349
XagI CCTNNNNNAGG 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.