MD08G1006100.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0021849
Physical Location & Seq
Forward (+)
521587 .. 523381
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1006100.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGAGGTGGATTGGACCCAACTACCACCGGAGCTCGCCGAATCAATCTCCAAAAAGCTCACAATCTACGCCGACTACCTCCGATTCCGGGTGGTGTGTCACAGCTGGCGAGCCTCCGTCCCCAAAACCCCGCACCACCTCCCTCCCCAGCTCCCCTGGCTGATGCTCCCCCAATCCCAGCCCAACCAATCCCACCGCGCCTTCTTCAACCTCTCTAACAGCAGAGTCCACTTCCTCCACCTTCCAGAAGCTTCTCACCGTAAGCGCCGCTGCGGCTCCTCCCACGGATGGCTCGTCATCCTCGACGAAACCCCTTCCGTCCTCCTCGTTAACCCCCTCACGCGCGCCAAGCGCCACCTCCCTCCGCTCTCCACCTTCCCCAACGTTGTTCGATTTGATTACTCCGATGTTGGCCGAGAATACGCCCTCGTATCCCCGTCTGGTGACGTCTACACGCGCAGCCTGACCCAAATGCGCGATTCGTTTCTGAAAAAGGTCGTCCTTTCTTCGAGTCCTCTGGAAGCCAGGGGCTTCACCTTCACCGCCGTAGCGATTGTCAATCAAACCGGCGACTTGGCGTTTTGCAGGGACGGAGACCAGACCTGGACTTTTATCGACGGCGCACAGTCGTATTCCGAGGATGTCGTATCTGCCGACGGTTTGTTTTACGCCGTCGACAAGAAGGGCACCGTGGCGGAGTGCGATGTTAATGGTCCGTCTCCGCCTAGGGTCAGGTTAATCCGAACGCCGAGACTAGAGGATGCCGACATGCGATATTTGGTGAGTTCAGGGGATGATTTGTTGTTGGTGAGTCGGTATTTGGAGATTGATTATGGTTTTCTGGTGGACAATGCAAATGTGAATTACAGGACAGCGAAATTTGATGTTTTCAGGATGAATTGGTTGGGGGAGAGGTGGGACAAGGTTGAGAACTTGGGTGATAGGATGGTGTTTATCGGCGAAAACTCATCGTTTTCATTGTCGGCGTCTGATTTTCCGGGAAGTCTTGGAAACTGCGTTTATTTCACAGACGATTATTCGGAATCAAACAATGAGAGCGGAGTCTGGGGATATGATTCGGGCATTTTCAAATTGTGGGATGGAACAATTCAAGAGTTGCCCCCTTATCCAAGGAATTCAAATTATGAGGTGCATTGGCCTGCCGGCTCTCTGCCTCTTTGGGTCACTCCAAATCCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

401

Amino Acids

45.36

Weight (kDa)

5.71

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 68 - 356 6.9e-67 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021849)

Species Orthologous Gene IDs
malus_domestica MD08G1006100.v1.1 MD15G1005500.v1.1
prunus_persica Prupe.1G358000_v2.0.a1
pyrus_communis pycom08g00470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 450
AccB1I GGYRCC 1 cut(s) 686
AccBSI CCGCTC 2 cut(s) 367, 1059
AccI GTMKAC 2 cut(s) 450, 675
AccII CGCG 5 cut(s) 198, 343, 345, 457, 477
AciI CCGC 9 cut(s) 131, 196, 268, 273, 365, 543, 695, 722, 1059
AclI AACGTT 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 878, 1135
AcyI GRCGYC 2 cut(s) 447, 986
AfiI CCNNNNNNNGG 2 cut(s) 88, 288
AgsI TTSAA 4 cut(s) 208, 1090, 1112, 1140
AjnI CCWGG 3 cut(s) 155, 524, 602
AluBI AGCT 5 cut(s) 34, 58, 105, 151, 251
AluI AGCT 5 cut(s) 34, 58, 105, 151, 251
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 3 cut(s) 588, 723, 745
AoxI GGCC 2 cut(s) 412, 1157
ApeKI GCWGC 2 cut(s) 270, 459
ApoI RAATTY 2 cut(s) 878, 1135
AspA2I CCTAGG 1 cut(s) 725
AspLEI GCGC 8 cut(s) 200, 267, 345, 347, 354, 459, 477, 623
AspS9I GGNCC 2 cut(s) 15, 713
AsuC2I CCSGG 2 cut(s) 89, 999
AsuHPI GGTGA 7 cut(s) 248, 455, 526, 532, 793, 820, 950
AvaII GGWCC 2 cut(s) 15, 713
AvrII CCTAGG 1 cut(s) 725
BaeGI GKGCMC 1 cut(s) 689
BanI GGYRCC 1 cut(s) 686
BanII GRGCYC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 257, 471
BccI CCATC 3 cut(s) 282, 940, 1094
BceAI ACGGC 3 cut(s) 530, 634, 656
BciT130I CCWGG 3 cut(s) 157, 526, 604
BciVI GTATCC 1 cut(s) 442
BcnI CCSGG 2 cut(s) 89, 999
BcoDI GTCTC 3 cut(s) 588, 723, 745
BfaI CTAG 2 cut(s) 726, 755
BfoI RGCGCY 2 cut(s) 268, 355
BfuI GTATCC 1 cut(s) 442
BglI GCCNNNNNGGC 1 cut(s) 273
BisI GCNGC 4 cut(s) 268, 271, 274, 460
BlnI CCTAGG 1 cut(s) 725
BlsI GCNGC 4 cut(s) 269, 272, 275, 461
Bme1390I CCNGG 5 cut(s) 89, 157, 526, 604, 999
Bme18I GGWCC 2 cut(s) 15, 713
BmgT120I GGNCC 2 cut(s) 15, 713
BmiI GGNNCC 3 cut(s) 17, 277, 688
BmrFI CCNGG 5 cut(s) 89, 157, 526, 604, 999
BmsI GCATC 2 cut(s) 153, 751
BpuMI CCSGG 2 cut(s) 89, 999
BsaHI GRCGYC 2 cut(s) 447, 986
BsaI GGTCTC 1 cut(s) 588
BsaJI CCNNGG 7 cut(s) 155, 283, 525, 636, 690, 725, 1130
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 288
Bse118I RCCGGY 2 cut(s) 566, 1163
BseBI CCWGG 3 cut(s) 157, 526, 604
BseDI CCNNGG 7 cut(s) 155, 283, 525, 636, 690, 725, 1130
BseGI GGATG 8 cut(s) 293, 297, 646, 766, 799, 900, 951, 1105
BseLI CCNNNNNNNGG 2 cut(s) 88, 288
BsePI GCGCGC 1 cut(s) 343
BseRI GAGGAG 2 cut(s) 268, 314
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 2 cut(s) 257, 471
BseYI CCCAGC 2 cut(s) 147, 177
Bsh1236I CGCG 5 cut(s) 198, 343, 345, 457, 477
BshFI GGCC 2 cut(s) 414, 1159
BshNI GGYRCC 1 cut(s) 686
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 5 cut(s) 29, 88, 567, 998, 1164
BslFI GGGAC 3 cut(s) 104, 602, 932
BslI CCNNNNNNNGG 2 cut(s) 88, 288
BsmAI GTCTC 3 cut(s) 588, 723, 745
BsmBI CGTCTC 1 cut(s) 723
BsmFI GGGAC 3 cut(s) 104, 602, 932
BsnI GGCC 2 cut(s) 414, 1159
Bso31I GGTCTC 1 cut(s) 588
Bsp1286I GDGCHC 2 cut(s) 36, 689
BspACI CCGC 9 cut(s) 131, 196, 268, 273, 365, 543, 695, 722, 1059
BspANI GGCC 2 cut(s) 414, 1159
BspFNI CGCG 5 cut(s) 198, 343, 345, 457, 477
BspLI GGNNCC 3 cut(s) 17, 277, 688
BspT107I GGYRCC 1 cut(s) 686
BspTNI GGTCTC 1 cut(s) 588
BsrBI CCGCTC 2 cut(s) 367, 1059
BsrFI RCCGGY 2 cut(s) 566, 1163
BssAI RCCGGY 2 cut(s) 566, 1163
BssECI CCNNGG 7 cut(s) 155, 283, 525, 636, 690, 725, 1130
BssHII GCGCGC 1 cut(s) 343
BssNI GRCGYC 2 cut(s) 447, 986
BssT1I CCWWGG 2 cut(s) 725, 1130
Bst2UI CCWGG 3 cut(s) 157, 526, 604
Bst4CI ACNGT 4 cut(s) 260, 627, 659, 691
BstACI GRCGYC 2 cut(s) 447, 986
BstC8I GCNNGC 6 cut(s) 36, 107, 111, 345, 1161, 1165
BstDSI CCRYGG 2 cut(s) 283, 690
BstF5I GGATG 8 cut(s) 293, 297, 646, 766, 799, 900, 951, 1105
BstFNI CGCG 5 cut(s) 198, 343, 345, 457, 477
BstH2I RGCGCY 2 cut(s) 268, 355
BstHHI GCGC 8 cut(s) 200, 267, 345, 347, 354, 459, 477, 623
BstMAI GTCTC 3 cut(s) 588, 723, 745
BstMWI GCNNNNNNNGC 4 cut(s) 157, 273, 349, 351
BstNI CCWGG 3 cut(s) 157, 526, 604
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 5 cut(s) 87, 155, 524, 602, 997
BstSLI GKGCMC 1 cut(s) 689
BstUI CGCG 5 cut(s) 198, 343, 345, 457, 477
BstV1I GCAGC 2 cut(s) 257, 471
BsuI GTATCC 1 cut(s) 442
BsuRI GGCC 2 cut(s) 414, 1159
BtgI CCRYGG 2 cut(s) 283, 690
BtgZI GCGATG 1 cut(s) 717
BtsCI GGATG 8 cut(s) 293, 297, 646, 766, 799, 900, 951, 1105
Cac8I GCNNGC 6 cut(s) 36, 107, 111, 345, 1161, 1165
CfoI GCGC 8 cut(s) 200, 267, 345, 347, 354, 459, 477, 623
Cfr10I RCCGGY 2 cut(s) 566, 1163
Cfr13I GGNCC 2 cut(s) 15, 713
CseI GACGC 1 cut(s) 975
CviAII CATG 2 cut(s) 769, 1197
EaeI YGGCCR 1 cut(s) 412
EciI GGCGGA 2 cut(s) 710, 711
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 725, 1130
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 588
Eco47I GGWCC 2 cut(s) 15, 713
Eco53kI GAGCTC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 1135
EcoRII CCWGG 3 cut(s) 155, 524, 602
EcoT14I CCWWGG 2 cut(s) 725, 1130
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 2 cut(s) 725, 1130
Esp3I CGTCTC 1 cut(s) 723
FaeI CATG 2 cut(s) 772, 1200
FaiI YATR 5 cut(s) 770, 834, 1074, 1146, 1198
FaqI GGGAC 3 cut(s) 104, 602, 932
FatI CATG 2 cut(s) 768, 1196
FauI CCCGC 1 cut(s) 138
FblI GTMKAC 2 cut(s) 450, 675
Fnu4HI GCNGC 4 cut(s) 268, 271, 274, 460
FokI GGATG 8 cut(s) 284, 300, 653, 773, 806, 907, 958, 1112
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 4 cut(s) 268, 271, 274, 460
FspBI CTAG 2 cut(s) 726, 755
GlaI GCGC 8 cut(s) 199, 266, 344, 346, 353, 458, 476, 622
GluI GCNGC 4 cut(s) 268, 271, 274, 460
GsaI CCCAGC 2 cut(s) 151, 181
HaeII RGCGCY 2 cut(s) 268, 355
HaeIII GGCC 2 cut(s) 414, 1159
HapII CCGG 5 cut(s) 29, 88, 567, 998, 1164
HgaI GACGC 1 cut(s) 975
HhaI GCGC 8 cut(s) 200, 267, 345, 347, 354, 459, 477, 623
Hin1I GRCGYC 2 cut(s) 447, 986
Hin1II CATG 2 cut(s) 772, 1200
Hin6I GCGC 8 cut(s) 198, 265, 343, 345, 352, 457, 475, 621
HinP1I GCGC 8 cut(s) 198, 265, 343, 345, 352, 457, 475, 621
HincII GTYRAC 2 cut(s) 331, 676
HindII GTYRAC 2 cut(s) 331, 676
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 9 cut(s) 41, 84, 225, 479, 511, 811, 1043, 1062, 1076
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 5 cut(s) 29, 88, 567, 998, 1164
HphI GGTGA 7 cut(s) 248, 455, 526, 532, 793, 820, 950
Hpy166II GTNNAC 5 cut(s) 229, 331, 451, 676, 847
Hpy188I TCNGA 7 cut(s) 83, 406, 489, 637, 743, 991, 1042
Hpy188III TCNNGA 4 cut(s) 245, 518, 892, 1112
Hpy8I GTNNAC 5 cut(s) 229, 331, 451, 676, 847
Hpy99I CGWCG 4 cut(s) 308, 620, 659, 677
HpyAV CCTTC 6 cut(s) 211, 251, 324, 385, 547, 676
HpyCH4III ACNGT 4 cut(s) 260, 627, 659, 691
HpyCH4IV ACGT 2 cut(s) 384, 447
HpyCH4V TGCA 3 cut(s) 585, 854, 1153
HpyF10VI GCNNNNNNNGC 4 cut(s) 157, 273, 349, 351
HpySE526I ACGT 2 cut(s) 384, 447
Hsp92I GRCGYC 2 cut(s) 447, 986
Hsp92II CATG 2 cut(s) 772, 1200
HspAI GCGC 8 cut(s) 198, 265, 343, 345, 352, 457, 475, 621
KroI GCCGGC 1 cut(s) 1163
KroNI GCCGGC 1 cut(s) 1165
KspAI GTTAAC 1 cut(s) 331
LmnI GCTCC 4 cut(s) 31, 156, 171, 281
Lsp1109I GCAGC 2 cut(s) 257, 471
LweI GCATC 2 cut(s) 153, 751
MaeI CTAG 2 cut(s) 726, 755
MaeII ACGT 2 cut(s) 384, 447
MaeIII GTNAC 3 cut(s) 98, 443, 1183
MbiI CCGCTC 2 cut(s) 367, 1059
MboII GAAGA 2 cut(s) 196, 498
MhlI GDGCHC 2 cut(s) 36, 689
MluCI AATT 7 cut(s) 862, 878, 898, 1091, 1107, 1135, 1141
MlyI GAGTC 4 cut(s) 234, 520, 820, 1071
MroNI GCCGGC 1 cut(s) 1163
MseI TTAA 3 cut(s) 330, 708, 737
MspA1I CMGCKG 2 cut(s) 105, 270
MspI CCGG 5 cut(s) 29, 88, 567, 998, 1164
MspR9I CCNGG 5 cut(s) 89, 157, 526, 604, 999
MvaI CCWGG 3 cut(s) 157, 526, 604
MvnI CGCG 5 cut(s) 198, 343, 345, 457, 477
MwoI GCNNNNNNNGC 4 cut(s) 157, 273, 349, 351
NaeI GCCGGC 1 cut(s) 1165
NciI CCSGG 2 cut(s) 89, 999
NgoMIV GCCGGC 1 cut(s) 1163
NlaIII CATG 2 cut(s) 772, 1200
NlaIV GGNNCC 3 cut(s) 17, 277, 688
NmeAIII GCCGAG 2 cut(s) 440, 774
NmuCI GTSAC 3 cut(s) 98, 443, 1183
NspI RCATGY 1 cut(s) 772
PauI GCGCGC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 3 cut(s) 291, 300, 651
PdiI GCCGGC 1 cut(s) 1165
PfeI GAWTC 5 cut(s) 41, 84, 479, 1043, 1076
PfoI TCCNGGA 1 cut(s) 997
PkrI GCNGC 4 cut(s) 269, 272, 275, 461
PleI GAGTC 4 cut(s) 233, 519, 819, 1070
PpsI GAGTC 4 cut(s) 233, 519, 819, 1070
Psp124BI GAGCTC 1 cut(s) 36
Psp1406I AACGTT 1 cut(s) 384
Psp6I CCWGG 3 cut(s) 155, 524, 602
PspFI CCCAGC 2 cut(s) 147, 177
PspGI CCWGG 3 cut(s) 155, 524, 602
PspN4I GGNNCC 3 cut(s) 17, 277, 688
PspPI GGNCC 2 cut(s) 15, 713
PteI GCGCGC 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 105
SacI GAGCTC 1 cut(s) 36
SalI GTCGAC 1 cut(s) 674
SaqAI TTAA 3 cut(s) 330, 708, 737
SatI GCNGC 4 cut(s) 268, 271, 274, 460
Sau96I GGNCC 2 cut(s) 15, 713
SchI GAGTC 4 cut(s) 234, 520, 820, 1071
ScrFI CCNGG 5 cut(s) 89, 157, 526, 604, 999
SduI GDGCHC 2 cut(s) 36, 689
SfaNI GCATC 2 cut(s) 153, 751
SinI GGWCC 2 cut(s) 15, 713
Sse9I AATT 7 cut(s) 862, 878, 898, 1091, 1107, 1135, 1141
SsiI CCGC 9 cut(s) 131, 196, 268, 273, 365, 543, 695, 722, 1059
SspMI CTAG 2 cut(s) 726, 755
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 5 cut(s) 87, 155, 524, 602, 997
StyI CCWWGG 2 cut(s) 725, 1130
TaaI ACNGT 4 cut(s) 260, 627, 659, 691
TaiI ACGT 2 cut(s) 387, 450
TaqI TCGA 5 cut(s) 303, 391, 509, 615, 675
TasI AATT 7 cut(s) 862, 878, 898, 1091, 1107, 1135, 1141
TauI GCSGC 2 cut(s) 270, 276
TfiI GAWTC 5 cut(s) 41, 84, 479, 1043, 1076
Tru1I TTAA 3 cut(s) 330, 708, 737
Tru9I TTAA 3 cut(s) 330, 708, 737
TseFI GTSAC 3 cut(s) 98, 443, 1183
TseI GCWGC 2 cut(s) 270, 459
Tsp45I GTSAC 3 cut(s) 98, 443, 1183
TspDTI ATGAA 2 cut(s) 911, 966
TspGWI ACGGA 5 cut(s) 106, 300, 307, 606, 705
VpaK11BI GGWCC 2 cut(s) 15, 713
XapI RAATTY 2 cut(s) 878, 1135
XceI RCATGY 1 cut(s) 772
XmaJI CCTAGG 1 cut(s) 725
XmiI GTMKAC 2 cut(s) 450, 675
XspI CTAG 2 cut(s) 726, 755
ZraI GACGTC 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.