MD15G1005500.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0021849
Physical Location & Seq
Forward (+)
324971 .. 326895
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1005500.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGGGGTGGATTGGACCCAACTACCACCAGAACTCGCCGAATCAATCTCCAAAAAGCTCACAATCTACGCCGACTACCTCCGATTCCGAGTTGTGTGTCACAGCTGGCGAGCCTCCGTCCCCAAAACCCCGCACCACCTCCCTCCCCAGCTCCCCTGGCTGATGCTCCCCCAATCCCAGCCCAACCAATCCCACCGGGCCTTCTTCAACCTCTCCAACAGCAGAGTCCACTTCCTCCACCTTCCAGAAGCTTCTCACCGCAAGCGTCGCTGCGGCTCCTCCCACGGATGGCTCGTCATCCTCGACGAAACACCCTCCGTCCTCCTCGTTAACCCCCTCACGCGCGCCAGGCGCCACCTCCCTCCGCTCTCCACCTTCCCCGACGTTGTTCGATTTGATTACTCTGAAGTTGGCCGAGAATACGCCCTCCGATCCCCGTCTGGTGACGTCTACACGCGCAGCCTGACCCAAATGCGCGATTCGTTTCTGAAAAAGGTCGTCCTTTCATCTAGTCCTCTGGAAGCCAACGGTTTCACTTTCACCGCCGTAGCGATTGTCAATCAAACCGGCGACTTGGCGTTTTGCAGGGAAGGAGACCAGTCTTGGACTTTGATCGACGGCGCACAGTCGTATTCCGAGGATGTCATATCTGTCAACGGCTTGTTTTACGCCGTCGACAAGAAGGGAACCGTGGTGGTGTGCGATGTTAATGGTCCGTCTCCGTCTAGGGTTGGGATTATCCGAACGCCGAGACTAGAGGAGGCCGACATGCGATATTTGGTGAGTTCAGGGGATGATTTGTTATTGGTGAGTCGGTATTTGGAGATTGATTACGGTTTTCTGGTGGACAGTATGAATGTAAATTACAAGACAGCAAAATTTGACCTTTTTAGGATGAATTGGGCGGGGGAGAGGTGGGACAAGGTTGAGAACTTGGGTGATAGGATGGTGTTTATTGGCGAAAACTCATCGTTTTCATTGTCGGCCTCTGATTTTCCAGGAAGTCTTGGAAATTGCGTTTATTTCACAGACGATTATTCGGAATCAAACAACGAGAGCGGAGTTGGGGGATATGATTCGGGCATTTTCAAATTATGGGATGGAACAATTCAAGAGTTGCCCCCTTATCCAGGGAATTCGAATTATGAGGTGCATTGGCCTGCCGGCTCTCTACCTCTTTGGGTAACTCCAAATCCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

401

Amino Acids

45.0

Weight (kDa)

5.71

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 68 - 358 5.5e-69 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021849)

Species Orthologous Gene IDs
malus_domestica MD08G1006100.v1.1 MD15G1005500.v1.1
prunus_persica Prupe.1G358000_v2.0.a1
pyrus_communis pycom08g00470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 450
AccB1I GGYRCC 1 cut(s) 351
AccBSI CCGCTC 2 cut(s) 367, 1059
AccI GTMKAC 2 cut(s) 450, 675
AccII CGCG 4 cut(s) 343, 345, 457, 477
AciI CCGC 7 cut(s) 131, 259, 273, 365, 543, 905, 1059
AclWI GGATC 1 cut(s) 426
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 878, 1135
AcuI CTGAAG 1 cut(s) 426
AcyI GRCGYC 2 cut(s) 352, 447
AfiI CCNNNNNNNGG 2 cut(s) 288, 1130
AgsI TTSAA 3 cut(s) 208, 1090, 1112
AjnI CCWGG 4 cut(s) 155, 347, 997, 1129
AluBI AGCT 4 cut(s) 58, 105, 151, 251
AluI AGCT 4 cut(s) 58, 105, 151, 251
Alw26I GTCTC 3 cut(s) 588, 723, 745
AlwI GGATC 1 cut(s) 426
AoxI GGCC 5 cut(s) 198, 412, 762, 984, 1157
ApeKI GCWGC 2 cut(s) 270, 459
ApoI RAATTY 2 cut(s) 878, 1135
AspLEI GCGC 6 cut(s) 345, 347, 354, 459, 477, 623
AspS9I GGNCC 3 cut(s) 15, 198, 713
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 6 cut(s) 248, 455, 532, 793, 820, 950
AsuII TTCGAA 1 cut(s) 1139
AvaII GGWCC 2 cut(s) 15, 713
BanI GGYRCC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 257, 471
BccI CCATC 3 cut(s) 282, 940, 1094
BceAI ACGGC 4 cut(s) 530, 634, 656, 673
BciT130I CCWGG 4 cut(s) 157, 349, 999, 1131
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 3 cut(s) 588, 723, 745
BfaI CTAG 3 cut(s) 510, 726, 755
BfoI RGCGCY 1 cut(s) 355
BisI GCNGC 3 cut(s) 271, 274, 460
BlsI GCNGC 3 cut(s) 272, 275, 461
Bme1390I CCNGG 5 cut(s) 157, 197, 349, 999, 1131
Bme18I GGWCC 2 cut(s) 15, 713
BmgT120I GGNCC 3 cut(s) 15, 198, 713
BmiI GGNNCC 4 cut(s) 17, 277, 353, 688
BmrFI CCNGG 5 cut(s) 157, 197, 349, 999, 1131
BmsI GCATC 1 cut(s) 153
Bpu14I TTCGAA 1 cut(s) 1139
BpuMI CCSGG 1 cut(s) 197
BsaHI GRCGYC 2 cut(s) 352, 447
BsaI GGTCTC 1 cut(s) 588
BsaJI CCNNGG 5 cut(s) 155, 283, 636, 690, 1130
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 1130
Bse118I RCCGGY 2 cut(s) 566, 1163
Bse1I ACTGG 1 cut(s) 598
BseBI CCWGG 4 cut(s) 157, 349, 999, 1131
BseDI CCNNGG 5 cut(s) 155, 283, 636, 690, 1130
BseGI GGATG 7 cut(s) 293, 297, 646, 799, 900, 951, 1105
BseLI CCNNNNNNNGG 2 cut(s) 288, 1130
BseNI ACTGG 1 cut(s) 598
BsePI GCGCGC 1 cut(s) 343
BseRI GAGGAG 3 cut(s) 268, 314, 773
BseXI GCAGC 2 cut(s) 257, 471
BseYI CCCAGC 2 cut(s) 147, 177
Bsh1236I CGCG 4 cut(s) 343, 345, 457, 477
BshFI GGCC 5 cut(s) 200, 414, 764, 986, 1159
BshNI GGYRCC 1 cut(s) 351
BsiSI CCGG 3 cut(s) 196, 567, 1164
BslFI GGGAC 2 cut(s) 104, 932
BslI CCNNNNNNNGG 2 cut(s) 288, 1130
BsmAI GTCTC 3 cut(s) 588, 723, 745
BsmBI CGTCTC 1 cut(s) 723
BsmFI GGGAC 2 cut(s) 104, 932
BsnI GGCC 5 cut(s) 200, 414, 764, 986, 1159
Bso31I GGTCTC 1 cut(s) 588
Bsp119I TTCGAA 1 cut(s) 1139
Bsp143I GATC 2 cut(s) 431, 612
BspACI CCGC 7 cut(s) 131, 259, 273, 365, 543, 905, 1059
BspANI GGCC 5 cut(s) 200, 414, 764, 986, 1159
BspFNI CGCG 4 cut(s) 343, 345, 457, 477
BspLI GGNNCC 4 cut(s) 17, 277, 353, 688
BspPI GGATC 1 cut(s) 426
BspT104I TTCGAA 1 cut(s) 1139
BspT107I GGYRCC 1 cut(s) 351
BspTNI GGTCTC 1 cut(s) 588
BsrBI CCGCTC 2 cut(s) 367, 1059
BsrFI RCCGGY 2 cut(s) 566, 1163
BsrI ACTGG 1 cut(s) 598
BssAI RCCGGY 2 cut(s) 566, 1163
BssECI CCNNGG 5 cut(s) 155, 283, 636, 690, 1130
BssHII GCGCGC 1 cut(s) 343
BssMI GATC 2 cut(s) 431, 612
BssNI GRCGYC 2 cut(s) 352, 447
Bst2UI CCWGG 4 cut(s) 157, 349, 999, 1131
Bst4CI ACNGT 5 cut(s) 530, 627, 691, 836, 851
BstACI GRCGYC 2 cut(s) 352, 447
BstBI TTCGAA 1 cut(s) 1139
BstC8I GCNNGC 6 cut(s) 107, 111, 263, 345, 1161, 1165
BstDSI CCRYGG 2 cut(s) 283, 690
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 7 cut(s) 293, 297, 646, 799, 900, 951, 1105
BstFNI CGCG 4 cut(s) 343, 345, 457, 477
BstH2I RGCGCY 1 cut(s) 355
BstHHI GCGC 6 cut(s) 345, 347, 354, 459, 477, 623
BstKTI GATC 2 cut(s) 434, 615
BstMAI GTCTC 3 cut(s) 588, 723, 745
BstMBI GATC 2 cut(s) 431, 612
BstMWI GCNNNNNNNGC 4 cut(s) 157, 267, 349, 351
BstNI CCWGG 4 cut(s) 157, 349, 999, 1131
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 5 cut(s) 155, 195, 347, 997, 1129
BstUI CGCG 4 cut(s) 343, 345, 457, 477
BstV1I GCAGC 2 cut(s) 257, 471
BsuRI GGCC 5 cut(s) 200, 414, 764, 986, 1159
BtgI CCRYGG 2 cut(s) 283, 690
BtgZI GCGATG 1 cut(s) 717
BtsCI GGATG 7 cut(s) 293, 297, 646, 799, 900, 951, 1105
Cac8I GCNNGC 6 cut(s) 107, 111, 263, 345, 1161, 1165
CfoI GCGC 6 cut(s) 345, 347, 354, 459, 477, 623
Cfr10I RCCGGY 2 cut(s) 566, 1163
Cfr13I GGNCC 3 cut(s) 15, 198, 713
CseI GACGC 1 cut(s) 254
CviAII CATG 2 cut(s) 769, 1197
DinI GGCGCC 1 cut(s) 353
DpnI GATC 2 cut(s) 433, 614
DpnII GATC 2 cut(s) 431, 612
EaeI YGGCCR 1 cut(s) 412
Eco31I GGTCTC 1 cut(s) 588
Eco47I GGWCC 2 cut(s) 15, 713
Eco57I CTGAAG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoRI GAATTC 1 cut(s) 1135
EcoRII CCWGG 4 cut(s) 155, 347, 997, 1129
EgeI GGCGCC 1 cut(s) 353
EheI GGCGCC 1 cut(s) 353
Esp3I CGTCTC 1 cut(s) 723
FaeI CATG 2 cut(s) 772, 1200
FaiI YATR 7 cut(s) 647, 770, 854, 1074, 1096, 1146, 1198
FaqI GGGAC 2 cut(s) 104, 932
FatI CATG 2 cut(s) 768, 1196
FauI CCCGC 2 cut(s) 138, 898
FblI GTMKAC 2 cut(s) 450, 675
Fnu4HI GCNGC 3 cut(s) 271, 274, 460
FokI GGATG 7 cut(s) 284, 300, 653, 806, 907, 958, 1112
Fsp4HI GCNGC 3 cut(s) 271, 274, 460
FspBI CTAG 3 cut(s) 510, 726, 755
GlaI GCGC 6 cut(s) 344, 346, 353, 458, 476, 622
GluI GCNGC 3 cut(s) 271, 274, 460
GsaI CCCAGC 2 cut(s) 151, 181
HaeII RGCGCY 1 cut(s) 355
HaeIII GGCC 5 cut(s) 200, 414, 764, 986, 1159
HapII CCGG 3 cut(s) 196, 567, 1164
HgaI GACGC 1 cut(s) 254
HhaI GCGC 6 cut(s) 345, 347, 354, 459, 477, 623
Hin1I GRCGYC 2 cut(s) 352, 447
Hin1II CATG 2 cut(s) 772, 1200
Hin6I GCGC 6 cut(s) 343, 345, 352, 457, 475, 621
HinP1I GCGC 6 cut(s) 343, 345, 352, 457, 475, 621
HincII GTYRAC 3 cut(s) 331, 655, 676
HindII GTYRAC 3 cut(s) 331, 655, 676
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 7 cut(s) 41, 84, 225, 479, 811, 1043, 1076
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 3 cut(s) 196, 567, 1164
HphI GGTGA 6 cut(s) 248, 455, 532, 793, 820, 950
Hpy166II GTNNAC 6 cut(s) 229, 331, 451, 655, 676, 847
Hpy188I TCNGA 9 cut(s) 83, 89, 406, 431, 489, 637, 743, 991, 1042
Hpy188III TCNNGA 3 cut(s) 245, 518, 1112
Hpy8I GTNNAC 6 cut(s) 229, 331, 451, 655, 676, 847
Hpy99I CGWCG 5 cut(s) 270, 308, 386, 620, 677
HpyAV CCTTC 5 cut(s) 211, 251, 385, 584, 676
HpyCH4III ACNGT 5 cut(s) 530, 627, 691, 836, 851
HpyCH4IV ACGT 2 cut(s) 384, 447
HpyCH4V TGCA 2 cut(s) 585, 1153
HpyF10VI GCNNNNNNNGC 4 cut(s) 157, 267, 349, 351
HpySE526I ACGT 2 cut(s) 384, 447
Hsp92I GRCGYC 2 cut(s) 352, 447
Hsp92II CATG 2 cut(s) 772, 1200
HspAI GCGC 6 cut(s) 343, 345, 352, 457, 475, 621
KasI GGCGCC 1 cut(s) 351
KroI GCCGGC 1 cut(s) 1163
KroNI GCCGGC 1 cut(s) 1165
KspAI GTTAAC 1 cut(s) 331
Kzo9I GATC 2 cut(s) 431, 612
LmnI GCTCC 3 cut(s) 156, 171, 281
Lsp1109I GCAGC 2 cut(s) 257, 471
LweI GCATC 1 cut(s) 153
MaeI CTAG 3 cut(s) 510, 726, 755
MaeII ACGT 2 cut(s) 384, 447
MaeIII GTNAC 3 cut(s) 98, 443, 1183
MalI GATC 2 cut(s) 433, 614
MbiI CCGCTC 2 cut(s) 367, 1059
MboI GATC 2 cut(s) 431, 612
MboII GAAGA 1 cut(s) 196
MluCI AATT 8 cut(s) 862, 878, 898, 1012, 1091, 1107, 1135, 1141
Mly113I GGCGCC 1 cut(s) 352
MlyI GAGTC 2 cut(s) 234, 820
MmeI TCCRAC 1 cut(s) 240
MroNI GCCGGC 1 cut(s) 1163
MseI TTAA 2 cut(s) 330, 708
MspA1I CMGCKG 1 cut(s) 105
MspI CCGG 3 cut(s) 196, 567, 1164
MspR9I CCNGG 5 cut(s) 157, 197, 349, 999, 1131
MvaI CCWGG 4 cut(s) 157, 349, 999, 1131
MvnI CGCG 4 cut(s) 343, 345, 457, 477
MwoI GCNNNNNNNGC 4 cut(s) 157, 267, 349, 351
NaeI GCCGGC 1 cut(s) 1165
NarI GGCGCC 1 cut(s) 352
NciI CCSGG 1 cut(s) 197
NdeII GATC 2 cut(s) 431, 612
NgoMIV GCCGGC 1 cut(s) 1163
NlaIII CATG 2 cut(s) 772, 1200
NlaIV GGNNCC 4 cut(s) 17, 277, 353, 688
NmeAIII GCCGAG 2 cut(s) 440, 774
NmuCI GTSAC 2 cut(s) 98, 443
NspI RCATGY 1 cut(s) 772
NspV TTCGAA 1 cut(s) 1139
PauI GCGCGC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 300
PdiI GCCGGC 1 cut(s) 1165
PfeI GAWTC 5 cut(s) 41, 84, 479, 1043, 1076
PfoI TCCNGGA 1 cut(s) 997
PkrI GCNGC 3 cut(s) 272, 275, 461
PleI GAGTC 2 cut(s) 233, 819
PluTI GGCGCC 1 cut(s) 355
PpsI GAGTC 2 cut(s) 233, 819
Psp6I CCWGG 4 cut(s) 155, 347, 997, 1129
PspFI CCCAGC 2 cut(s) 147, 177
PspGI CCWGG 4 cut(s) 155, 347, 997, 1129
PspN4I GGNNCC 4 cut(s) 17, 277, 353, 688
PspPI GGNCC 3 cut(s) 15, 198, 713
PteI GCGCGC 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 105
SalI GTCGAC 1 cut(s) 674
SaqAI TTAA 2 cut(s) 330, 708
SatI GCNGC 3 cut(s) 271, 274, 460
Sau3AI GATC 2 cut(s) 431, 612
Sau96I GGNCC 3 cut(s) 15, 198, 713
SchI GAGTC 2 cut(s) 234, 820
ScrFI CCNGG 5 cut(s) 157, 197, 349, 999, 1131
SfaNI GCATC 1 cut(s) 153
SfoI GGCGCC 1 cut(s) 353
SfuI TTCGAA 1 cut(s) 1139
SinI GGWCC 2 cut(s) 15, 713
Sse9I AATT 8 cut(s) 862, 878, 898, 1012, 1091, 1107, 1135, 1141
SsiI CCGC 7 cut(s) 131, 259, 273, 365, 543, 905, 1059
SspDI GGCGCC 1 cut(s) 351
SspMI CTAG 3 cut(s) 510, 726, 755
StyD4I CCNGG 5 cut(s) 155, 195, 347, 997, 1129
TaaI ACNGT 5 cut(s) 530, 627, 691, 836, 851
TaiI ACGT 2 cut(s) 387, 450
TaqI TCGA 5 cut(s) 303, 391, 615, 675, 1139
TasI AATT 8 cut(s) 862, 878, 898, 1012, 1091, 1107, 1135, 1141
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 5 cut(s) 41, 84, 479, 1043, 1076
Tru1I TTAA 2 cut(s) 330, 708
Tru9I TTAA 2 cut(s) 330, 708
TseFI GTSAC 2 cut(s) 98, 443
TseI GCWGC 2 cut(s) 270, 459
Tsp45I GTSAC 2 cut(s) 98, 443
TspDTI ATGAA 4 cut(s) 495, 869, 911, 966
TspGWI ACGGA 5 cut(s) 106, 300, 307, 705, 711
VpaK11BI GGWCC 2 cut(s) 15, 713
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 2 cut(s) 878, 1135
XceI RCATGY 1 cut(s) 772
XmiI GTMKAC 2 cut(s) 450, 675
XspI CTAG 3 cut(s) 510, 726, 755
ZraI GACGTC 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.