MD08G1163300.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0022391
Physical Location & Seq
Forward (+)
19215398 .. 19216984
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1163300.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGCAATGTTACCAGTGCCTAGCTAGGATAAGCAGCTCCTTAACAATGGAAGAAATCAATCCATCATACATTCTATCAGAAGAATACAGGCCTAAACACGTAATCACCGAAGGCGAAGGAATCCCGTTGATCGATCTCACTCCGATAACCAACCGAGAAATGTCAGCCGGCTCTAACAGTAAGGCAGTGGAAGAGCTTGCCGCTAAGATAGGGGAGGCTTGCAGGACATGGGGGTTCTTCACGGTGATCAACCATGGCGTGCCTTCGGATATTCGGAGAAGAATTATGGAGGCGTCGAGGAAGTTCTTCGCGCTGCCGCTGGAGGAGAAGAAGAAGGTGAGCAGAGAGGCTCATAACACAGCAGGGTTTCATAACGATGAGCATAGCAAAGACTTCAAGGACTGGAAAGAAGTTTATGATTTTTATGTAAATGATGGGATGCTAATGCCGGCTTCCCATGAACCCGATGACCCCGAAATTGTTCCATGGTACACTCCGTGGCCCGAGAACCTATCCAAGTTCAGGGAAACATGTGAAGAATATGGTCGAGCATGTGAGAAGTTGTTTTTCAATCTGCTGGAACTGGTAAGCCTCAGCTTAGGCTTACCTCCTACGAGGTTGCATGGATACTTCGAGAATCAGGCAAGCTTTGCCCGGCTCAACTACTACGCTCCGTGCCCCAAACCGGACCTTGTTCTTGGCACCGGTGGACATAGGGACCCCAGCGCTCTTACCGTCCTTGCTCAAGAAGATGTTGAAGGCCTCGATGTGCTGCGAAAATCAGACGGAGCGTGGGTTCGCGTCAAGCCTGTCCCGGATTCCTTTGTCATCAACGTTGGTGATGTTCTTCAGGTATGGAGCAATGATCTTTACGAAAGCGTCGAGCATCGAGCCATGGTGCACGCAGAAACGGAGAGGTATTCGATCCCCATATTTTTTCACCCATCTCACGACATAACAATGAAGCCGTTGGATGAATTGGTCGACGAAAGAAGCCCGGCGAAGTATCCAGAATACAAGGCAGGGAAGTGGTTGAATCTGAGGATGTTTAACAATTACAAGAAACATGGCTTCTATATGCGGATGACTGACTACAAGATTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

370

Amino Acids

42.44

Weight (kDa)

5.6

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 41 - 155 3.1e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 219 - 315 2.6e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022391)

Species Orthologous Gene IDs
malus_domestica MD08G1163300.v1.1 MD08G1163600.v1.1
prunus_persica Prupe.1G499300_v2.0.a1 Prupe.1G499300_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 701
AccI GTMKAC 1 cut(s) 984
AccII CGCG 2 cut(s) 311, 801
AciI CCGC 3 cut(s) 201, 317, 1081
AclI AACGTT 1 cut(s) 834
AclWI GGATC 1 cut(s) 919
AcuI CTGAAG 1 cut(s) 833
AcyI GRCGYC 1 cut(s) 293
AdeI CACNNNGTG 1 cut(s) 498
AfaI GTAC 1 cut(s) 491
AfeI AGCGCT 1 cut(s) 727
AfiI CCNNNNNNNGG 2 cut(s) 522, 685
AflIII ACRYGT 2 cut(s) 97, 530
AgeI ACCGGT 1 cut(s) 704
AgsI TTSAA 4 cut(s) 397, 571, 758, 1036
AloI GAACNNNNNNTCC 2 cut(s) 780, 812
AluBI AGCT 5 cut(s) 23, 36, 196, 597, 648
AluI AGCT 5 cut(s) 23, 36, 196, 597, 648
Alw21I GWGCWC 1 cut(s) 903
Alw44I GTGCAC 1 cut(s) 899
AlwI GGATC 1 cut(s) 919
Ama87I CYCGRG 1 cut(s) 503
Aor51HI AGCGCT 1 cut(s) 727
AoxI GGCC 3 cut(s) 89, 500, 760
ApaLI GTGCAC 1 cut(s) 899
ApeKI GCWGC 4 cut(s) 33, 313, 772, 1103
AsiGI ACCGGT 1 cut(s) 704
Asp700I GAANNNNTTC 2 cut(s) 305, 480
AspLEI GCGC 2 cut(s) 313, 728
AspS9I GGNCC 3 cut(s) 501, 688, 718
AsuC2I CCSGG 3 cut(s) 655, 815, 998
AsuHPI GGTGA 5 cut(s) 97, 256, 349, 851, 932
AvaI CYCGRG 1 cut(s) 503
AvaII GGWCC 2 cut(s) 688, 718
BaeGI GKGCMC 2 cut(s) 680, 903
BanI GGYRCC 1 cut(s) 701
Bbv12I GWGCWC 1 cut(s) 903
BbvCI CCTCAGC 1 cut(s) 593
BbvI GCAGC 4 cut(s) 45, 300, 759, 1090
BccI CCATC 3 cut(s) 70, 428, 952
BceAI ACGGC 1 cut(s) 952
BciVI GTATCC 2 cut(s) 620, 1017
BclI TGATCA 1 cut(s) 246
BcnI CCSGG 3 cut(s) 655, 815, 998
BfaI CTAG 2 cut(s) 20, 24
BfoI RGCGCY 1 cut(s) 729
BfuI GTATCC 2 cut(s) 620, 1017
BisI GCNGC 6 cut(s) 34, 201, 314, 317, 773, 1104
BlsI GCNGC 6 cut(s) 35, 202, 315, 318, 774, 1105
Bme1390I CCNGG 3 cut(s) 655, 815, 998
Bme18I GGWCC 2 cut(s) 688, 718
BmeT110I CYCGRG 1 cut(s) 503
BmgT120I GGNCC 3 cut(s) 501, 688, 718
BmiI GGNNCC 3 cut(s) 703, 719, 720
BmrFI CCNGG 3 cut(s) 655, 815, 998
BmsI GCATC 2 cut(s) 429, 895
BpmI CTGGAG 1 cut(s) 341
Bpu10I CCTNAGC 2 cut(s) 593, 598
BpuEI CTTGAG 1 cut(s) 729
BpuMI CCSGG 3 cut(s) 655, 815, 998
Bsa29I ATCGAT 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 293
BsaJI CCNNGG 4 cut(s) 253, 485, 497, 894
BsaWI WCCGGW 2 cut(s) 685, 704
BsaXI ACNNNNNCTCC 2 cut(s) 780, 810
Bsc4I CCNNNNNNNGG 2 cut(s) 522, 685
Bse118I RCCGGY 3 cut(s) 167, 448, 704
Bse1I ACTGG 3 cut(s) 13, 407, 588
Bse3DI GCAATG 2 cut(s) 11, 868
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 4 cut(s) 253, 485, 497, 894
BseGI GGATG 4 cut(s) 444, 979, 1050, 1089
BseLI CCNNNNNNNGG 2 cut(s) 522, 685
BseMI GCAATG 2 cut(s) 11, 868
BseMII CTCAG 2 cut(s) 607, 1031
BseNI ACTGG 3 cut(s) 13, 407, 588
BseRI GAGGAG 1 cut(s) 338
BseSI GKGCMC 2 cut(s) 680, 903
BseXI GCAGC 4 cut(s) 45, 300, 759, 1090
BseYI CCCAGC 1 cut(s) 722
Bsh1236I CGCG 2 cut(s) 311, 801
BshFI GGCC 3 cut(s) 91, 502, 762
BshNI GGYRCC 1 cut(s) 701
BshTI ACCGGT 1 cut(s) 704
BshVI ATCGAT 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 903
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 7 cut(s) 168, 449, 655, 686, 705, 815, 998
BslFI GGGAC 2 cut(s) 731, 797
BslI CCNNNNNNNGG 2 cut(s) 522, 685
BsmFI GGGAC 2 cut(s) 731, 797
BsnI GGCC 3 cut(s) 91, 502, 762
BsoBI CYCGRG 1 cut(s) 503
Bsp1286I GDGCHC 2 cut(s) 680, 903
Bsp143I GATC 5 cut(s) 129, 133, 246, 865, 924
Bsp19I CCATGG 3 cut(s) 253, 485, 894
BspACI CCGC 3 cut(s) 201, 317, 1081
BspANI GGCC 3 cut(s) 91, 502, 762
BspCNI CTCAG 2 cut(s) 606, 1032
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 2 cut(s) 311, 801
BspLI GGNNCC 3 cut(s) 703, 719, 720
BspPI GGATC 1 cut(s) 919
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 701
BsrDI GCAATG 2 cut(s) 11, 868
BsrFI RCCGGY 3 cut(s) 167, 448, 704
BsrI ACTGG 3 cut(s) 13, 407, 588
BssAI RCCGGY 3 cut(s) 167, 448, 704
BssECI CCNNGG 4 cut(s) 253, 485, 497, 894
BssMI GATC 5 cut(s) 129, 133, 246, 865, 924
BssNI GRCGYC 1 cut(s) 293
BssT1I CCWWGG 3 cut(s) 253, 485, 894
Bst4CI ACNGT 3 cut(s) 179, 244, 736
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 650
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 7 cut(s) 169, 198, 220, 260, 450, 646, 903
BstDEI CTNAG 5 cut(s) 204, 593, 598, 1040, 1107
BstDSI CCRYGG 4 cut(s) 253, 485, 497, 894
BstF5I GGATG 4 cut(s) 444, 979, 1050, 1089
BstFNI CGCG 2 cut(s) 311, 801
BstH2I RGCGCY 1 cut(s) 729
BstHHI GCGC 2 cut(s) 313, 728
BstKTI GATC 5 cut(s) 132, 136, 249, 868, 927
BstMBI GATC 5 cut(s) 129, 133, 246, 865, 924
BstMWI GCNNNNNNNGC 1 cut(s) 650
BstNSI RCATGY 2 cut(s) 534, 555
BstSCI CCNGG 3 cut(s) 653, 813, 996
BstSLI GKGCMC 2 cut(s) 680, 903
BstUI CGCG 2 cut(s) 311, 801
BstV1I GCAGC 4 cut(s) 45, 300, 759, 1090
Bsu15I ATCGAT 1 cut(s) 132
BsuI GTATCC 2 cut(s) 620, 1017
BsuRI GGCC 3 cut(s) 91, 502, 762
BsuTUI ATCGAT 1 cut(s) 132
BtgI CCRYGG 4 cut(s) 253, 485, 497, 894
BtsCI GGATG 4 cut(s) 444, 979, 1050, 1089
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 20, 192
Cac8I GCNNGC 7 cut(s) 169, 198, 220, 260, 450, 646, 903
CfoI GCGC 2 cut(s) 313, 728
Cfr10I RCCGGY 3 cut(s) 167, 448, 704
Cfr13I GGNCC 3 cut(s) 501, 688, 718
ClaI ATCGAT 1 cut(s) 132
CseI GACGC 3 cut(s) 282, 790, 868
Csp6I GTAC 1 cut(s) 490
CspAI ACCGGT 1 cut(s) 704
CviAII CATG 9 cut(s) 228, 254, 458, 486, 531, 552, 623, 895, 1067
CviQI GTAC 1 cut(s) 490
DdeI CTNAG 5 cut(s) 204, 593, 598, 1040, 1107
DpnI GATC 5 cut(s) 131, 135, 248, 867, 926
DpnII GATC 5 cut(s) 129, 133, 246, 865, 924
DraIII CACNNNGTG 1 cut(s) 498
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco130I CCWWGG 3 cut(s) 253, 485, 894
Eco147I AGGCCT 2 cut(s) 91, 762
Eco47I GGWCC 2 cut(s) 688, 718
Eco47III AGCGCT 1 cut(s) 727
Eco57I CTGAAG 1 cut(s) 833
Eco88I CYCGRG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 718
EcoT14I CCWWGG 3 cut(s) 253, 485, 894
ErhI CCWWGG 3 cut(s) 253, 485, 894
FaeI CATG 9 cut(s) 231, 257, 461, 489, 534, 555, 626, 898, 1070
FaqI GGGAC 2 cut(s) 731, 797
FatI CATG 9 cut(s) 227, 253, 457, 485, 530, 551, 622, 894, 1066
FbaI TGATCA 1 cut(s) 246
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 6 cut(s) 34, 201, 314, 317, 773, 1104
FokI GGATG 4 cut(s) 451, 986, 1057, 1096
Fsp4HI GCNGC 6 cut(s) 34, 201, 314, 317, 773, 1104
FspBI CTAG 2 cut(s) 20, 24
GlaI GCGC 2 cut(s) 312, 727
GluI GCNGC 6 cut(s) 34, 201, 314, 317, 773, 1104
GsaI CCCAGC 1 cut(s) 726
GsuI CTGGAG 1 cut(s) 341
HaeII RGCGCY 1 cut(s) 729
HaeIII GGCC 3 cut(s) 91, 502, 762
HapII CCGG 7 cut(s) 168, 449, 655, 686, 705, 815, 998
HgaI GACGC 3 cut(s) 282, 790, 868
HhaI GCGC 2 cut(s) 313, 728
Hin1I GRCGYC 1 cut(s) 293
Hin1II CATG 9 cut(s) 231, 257, 461, 489, 534, 555, 626, 898, 1070
Hin6I GCGC 2 cut(s) 311, 726
HinP1I GCGC 2 cut(s) 311, 726
HincII GTYRAC 1 cut(s) 985
HindII GTYRAC 1 cut(s) 985
HindIII AAGCTT 1 cut(s) 646
HinfI GANTC 4 cut(s) 120, 637, 818, 1036
HpaII CCGG 7 cut(s) 168, 449, 655, 686, 705, 815, 998
HphI GGTGA 5 cut(s) 97, 256, 349, 851, 932
Hpy166II GTNNAC 4 cut(s) 492, 710, 901, 985
Hpy188I TCNGA 6 cut(s) 79, 144, 268, 276, 784, 1041
Hpy188III TCNNGA 4 cut(s) 634, 746, 950, 1010
Hpy8I GTNNAC 4 cut(s) 492, 710, 901, 985
Hpy99I CGWCG 3 cut(s) 298, 884, 989
HpyAV CCTTC 5 cut(s) 104, 110, 273, 328, 752
HpyCH4III ACNGT 3 cut(s) 179, 244, 736
HpyCH4IV ACGT 2 cut(s) 99, 834
HpyCH4V TGCA 4 cut(s) 4, 222, 622, 901
HpyF10VI GCNNNNNNNGC 1 cut(s) 650
HpyF3I CTNAG 5 cut(s) 204, 593, 598, 1040, 1107
HpySE526I ACGT 2 cut(s) 99, 834
Hsp92I GRCGYC 1 cut(s) 293
Hsp92II CATG 9 cut(s) 231, 257, 461, 489, 534, 555, 626, 898, 1070
HspAI GCGC 2 cut(s) 311, 726
KflI GGGWCCC 1 cut(s) 718
KroI GCCGGC 2 cut(s) 167, 448
KroNI GCCGGC 2 cut(s) 169, 450
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 5 cut(s) 129, 133, 246, 865, 924
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 4 cut(s) 41, 676, 788, 858
Lsp1109I GCAGC 4 cut(s) 45, 300, 759, 1090
LweI GCATC 2 cut(s) 429, 895
MaeI CTAG 2 cut(s) 20, 24
MaeII ACGT 2 cut(s) 99, 834
MaeIII GTNAC 1 cut(s) 8
MalI GATC 5 cut(s) 131, 135, 248, 867, 926
MboI GATC 5 cut(s) 129, 133, 246, 865, 924
MhlI GDGCHC 2 cut(s) 680, 903
MluCI AATT 4 cut(s) 282, 477, 977, 1054
MmeI TCCRAC 1 cut(s) 951
MroNI GCCGGC 2 cut(s) 167, 448
MroXI GAANNNNTTC 2 cut(s) 305, 480
MseI TTAA 2 cut(s) 41, 1050
MslI CAYNNNNRTG 2 cut(s) 375, 959
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 7 cut(s) 168, 449, 655, 686, 705, 815, 998
MspR9I CCNGG 3 cut(s) 655, 815, 998
MvnI CGCG 2 cut(s) 311, 801
MwoI GCNNNNNNNGC 1 cut(s) 650
NaeI GCCGGC 2 cut(s) 169, 450
NciI CCSGG 3 cut(s) 655, 815, 998
NcoI CCATGG 3 cut(s) 253, 485, 894
NdeII GATC 5 cut(s) 129, 133, 246, 865, 924
NgoMIV GCCGGC 2 cut(s) 167, 448
NlaIII CATG 9 cut(s) 231, 257, 461, 489, 534, 555, 626, 898, 1070
NlaIV GGNNCC 3 cut(s) 703, 719, 720
NspI RCATGY 2 cut(s) 534, 555
PceI AGGCCT 2 cut(s) 91, 762
PciI ACATGT 1 cut(s) 530
PciSI GCTCTTC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 879
PdiI GCCGGC 2 cut(s) 169, 450
PdmI GAANNNNTTC 2 cut(s) 305, 480
PfeI GAWTC 4 cut(s) 120, 637, 818, 1036
PfoI TCCNGGA 1 cut(s) 813
PinAI ACCGGT 1 cut(s) 704
PkrI GCNGC 6 cut(s) 35, 202, 315, 318, 774, 1105
Ppu21I YACGTR 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 718
PscI ACATGT 1 cut(s) 530
Psp1406I AACGTT 1 cut(s) 834
Psp5II RGGWCCY 1 cut(s) 718
PspFI CCCAGC 1 cut(s) 722
PspN4I GGNNCC 3 cut(s) 703, 719, 720
PspPI GGNCC 3 cut(s) 501, 688, 718
PspPPI RGGWCCY 1 cut(s) 718
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
RseI CAYNNNNRTG 2 cut(s) 375, 959
SalI GTCGAC 1 cut(s) 983
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 41, 1050
SatI GCNGC 6 cut(s) 34, 201, 314, 317, 773, 1104
Sau3AI GATC 5 cut(s) 129, 133, 246, 865, 924
Sau96I GGNCC 3 cut(s) 501, 688, 718
ScrFI CCNGG 3 cut(s) 655, 815, 998
SduI GDGCHC 2 cut(s) 680, 903
SfaNI GCATC 2 cut(s) 429, 895
SgrAI CRCCGGYG 1 cut(s) 704
SinI GGWCC 2 cut(s) 688, 718
SmiMI CAYNNNNRTG 2 cut(s) 375, 959
SmlI CTYRAG 1 cut(s) 744
SmoI CTYRAG 1 cut(s) 744
Sse9I AATT 4 cut(s) 282, 477, 977, 1054
SseBI AGGCCT 2 cut(s) 91, 762
SsiI CCGC 3 cut(s) 201, 317, 1081
SspMI CTAG 2 cut(s) 20, 24
StuI AGGCCT 2 cut(s) 91, 762
StyD4I CCNGG 3 cut(s) 653, 813, 996
StyI CCWWGG 3 cut(s) 253, 485, 894
TaaI ACNGT 3 cut(s) 179, 244, 736
TaiI ACGT 2 cut(s) 102, 837
TaqI TCGA 9 cut(s) 132, 296, 547, 633, 765, 882, 889, 923, 984
TasI AATT 4 cut(s) 282, 477, 977, 1054
TauI GCSGC 2 cut(s) 203, 319
TfiI GAWTC 4 cut(s) 120, 637, 818, 1036
Tru1I TTAA 2 cut(s) 41, 1050
Tru9I TTAA 2 cut(s) 41, 1050
TscAI CASTG 2 cut(s) 20, 192
TseI GCWGC 4 cut(s) 33, 313, 772, 1103
TspDTI ATGAA 4 cut(s) 359, 474, 977, 990
TspGWI ACGGA 4 cut(s) 486, 663, 801, 926
TspRI CASTG 2 cut(s) 20, 192
VneI GTGCAC 1 cut(s) 899
VpaK11BI GGWCC 2 cut(s) 688, 718
XceI RCATGY 2 cut(s) 534, 555
XmiI GTMKAC 1 cut(s) 984
XmnI GAANNNNTTC 2 cut(s) 305, 480
XspI CTAG 2 cut(s) 20, 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.