MD08G1163600.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0022391
Physical Location & Seq
Forward (+)
19235734 .. 19236984
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1163600.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGCAATGTTACCAGTGCCTAGCTAGGATAAGCAGCTCCTTAACAATGGAAGAAATCAATCCATCATACATTCTATCAGAAGAATACAGGCCTAAACACGTAATCACCGAAGGCGAAGGAATCCCGTTGATCGATCTCACTCCGATAACCAACCGAGAAATGTCAGCCGGCTCTAACAGTAAGGCAGTGGAAGAGCTTGCCGCTAAGATAGGGGAGGCTTGCAGGACATGGGGGTTCTTCACGGTGATCAACCATGGCGTGCCTTCGGATATTCGGAGAAGAATTATGGAGGCGTCGAGGAAGTTCTTCGCGCTGCCGCTGGAGGAGAAGAAGAAGGTGAGCAGAGAGGCTCATAACACAGCAGGGTTTCATAACGATGAGCATAGCAAAGACTTCAAGGACTGGAAAGAAGTTTATGATTTTTATGTAAATGATGGGGAAACATGTGAAGAATATGGTCGAGCATGTGAGAAGTTGTTTTTCAATCTGCTGGAACTGGTAAGCCTCAGCTTAGGCTTACCTCCTACGAGGTTGCATGGATACTTCGAGAATCAGGCAAGCTTTGCCCGGCTCAACTACTACGCTCCGTGCCCCAAACCGGACCTTGTTCTTGGCACCGGTGGACATAGGGACCCCAGCGCTCTTACCGTCCTTGCTCAAGAAGATGTTGAAGGCCTCGATGTGCTGCGAAAATCAGACGGAGCGTGGGTTCGCGTCAAGCCTGTCCCGGATTCCTTTGTCATCAACGTTGGTGATGTTCTTCAGGTATGGAGCAATGATCTTTACGAAAGCGTCGAGCATCGAGCCATGGTGCACGCAGAAACGGAGAGGTATTCGATCCCCATATTTTTTCACCCATCTCACGACATAACAATGAAGCCGTTGGATGAATTGGTCGACGAAAGAAGCCCGGCGAAGTATCCAGAATACAAGGCAGGGAAGTGGTTGAATCTGAGGATGTTTAACAATTACAAGAAACATGGCTTCTATATGCGGATGACTGACTACAAGATTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

341

Amino Acids

38.97

Weight (kDa)

5.78

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 41 - 145 5.9e-27 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 190 - 286 2.2e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022391)

Species Orthologous Gene IDs
malus_domestica MD08G1163300.v1.1 MD08G1163600.v1.1
prunus_persica Prupe.1G499300_v2.0.a1 Prupe.1G499300_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 614
AccI GTMKAC 1 cut(s) 897
AccII CGCG 2 cut(s) 311, 714
AciI CCGC 3 cut(s) 201, 317, 994
AclI AACGTT 1 cut(s) 747
AclWI GGATC 1 cut(s) 832
AcuI CTGAAG 1 cut(s) 746
AcyI GRCGYC 1 cut(s) 293
AfeI AGCGCT 1 cut(s) 640
AfiI CCNNNNNNNGG 1 cut(s) 598
AflIII ACRYGT 2 cut(s) 97, 443
AgeI ACCGGT 1 cut(s) 617
AgsI TTSAA 4 cut(s) 397, 484, 671, 949
AloI GAACNNNNNNTCC 2 cut(s) 693, 725
AluBI AGCT 5 cut(s) 23, 36, 196, 510, 561
AluI AGCT 5 cut(s) 23, 36, 196, 510, 561
Alw21I GWGCWC 1 cut(s) 816
Alw44I GTGCAC 1 cut(s) 812
AlwI GGATC 1 cut(s) 832
Aor51HI AGCGCT 1 cut(s) 640
AoxI GGCC 2 cut(s) 89, 673
ApaLI GTGCAC 1 cut(s) 812
ApeKI GCWGC 4 cut(s) 33, 313, 685, 1016
AsiGI ACCGGT 1 cut(s) 617
Asp700I GAANNNNTTC 1 cut(s) 305
AspLEI GCGC 2 cut(s) 313, 641
AspS9I GGNCC 2 cut(s) 601, 631
AsuC2I CCSGG 3 cut(s) 568, 728, 911
AsuHPI GGTGA 5 cut(s) 97, 256, 349, 764, 845
AvaII GGWCC 2 cut(s) 601, 631
BaeGI GKGCMC 2 cut(s) 593, 816
BanI GGYRCC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 816
BbvCI CCTCAGC 1 cut(s) 506
BbvI GCAGC 4 cut(s) 45, 300, 672, 1003
BccI CCATC 3 cut(s) 70, 428, 865
BceAI ACGGC 1 cut(s) 865
BciVI GTATCC 2 cut(s) 533, 930
BclI TGATCA 1 cut(s) 246
BcnI CCSGG 3 cut(s) 568, 728, 911
BfaI CTAG 2 cut(s) 20, 24
BfoI RGCGCY 1 cut(s) 642
BfuI GTATCC 2 cut(s) 533, 930
BisI GCNGC 6 cut(s) 34, 201, 314, 317, 686, 1017
BlsI GCNGC 6 cut(s) 35, 202, 315, 318, 687, 1018
Bme1390I CCNGG 3 cut(s) 568, 728, 911
Bme18I GGWCC 2 cut(s) 601, 631
BmgT120I GGNCC 2 cut(s) 601, 631
BmiI GGNNCC 3 cut(s) 616, 632, 633
BmrFI CCNGG 3 cut(s) 568, 728, 911
BmsI GCATC 1 cut(s) 808
BpmI CTGGAG 1 cut(s) 341
Bpu10I CCTNAGC 2 cut(s) 506, 511
BpuEI CTTGAG 1 cut(s) 642
BpuMI CCSGG 3 cut(s) 568, 728, 911
Bsa29I ATCGAT 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 253, 807
BsaWI WCCGGW 2 cut(s) 598, 617
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 1 cut(s) 598
Bse118I RCCGGY 2 cut(s) 167, 617
Bse1I ACTGG 3 cut(s) 13, 407, 501
Bse3DI GCAATG 2 cut(s) 11, 781
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 253, 807
BseGI GGATG 3 cut(s) 892, 963, 1002
BseLI CCNNNNNNNGG 1 cut(s) 598
BseMI GCAATG 2 cut(s) 11, 781
BseMII CTCAG 2 cut(s) 520, 944
BseNI ACTGG 3 cut(s) 13, 407, 501
BseRI GAGGAG 1 cut(s) 338
BseSI GKGCMC 2 cut(s) 593, 816
BseXI GCAGC 4 cut(s) 45, 300, 672, 1003
BseYI CCCAGC 1 cut(s) 635
Bsh1236I CGCG 2 cut(s) 311, 714
BshFI GGCC 2 cut(s) 91, 675
BshNI GGYRCC 1 cut(s) 614
BshTI ACCGGT 1 cut(s) 617
BshVI ATCGAT 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 816
BsiSI CCGG 6 cut(s) 168, 568, 599, 618, 728, 911
BslFI GGGAC 2 cut(s) 644, 710
BslI CCNNNNNNNGG 1 cut(s) 598
BsmFI GGGAC 2 cut(s) 644, 710
BsnI GGCC 2 cut(s) 91, 675
Bsp1286I GDGCHC 2 cut(s) 593, 816
Bsp143I GATC 5 cut(s) 129, 133, 246, 778, 837
Bsp19I CCATGG 2 cut(s) 253, 807
BspACI CCGC 3 cut(s) 201, 317, 994
BspANI GGCC 2 cut(s) 91, 675
BspCNI CTCAG 2 cut(s) 519, 945
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 2 cut(s) 311, 714
BspLI GGNNCC 3 cut(s) 616, 632, 633
BspPI GGATC 1 cut(s) 832
BspQI GCTCTTC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 614
BsrDI GCAATG 2 cut(s) 11, 781
BsrFI RCCGGY 2 cut(s) 167, 617
BsrI ACTGG 3 cut(s) 13, 407, 501
BssAI RCCGGY 2 cut(s) 167, 617
BssECI CCNNGG 2 cut(s) 253, 807
BssMI GATC 5 cut(s) 129, 133, 246, 778, 837
BssNI GRCGYC 1 cut(s) 293
BssT1I CCWWGG 2 cut(s) 253, 807
Bst4CI ACNGT 3 cut(s) 179, 244, 649
Bst6I CTCTTC 1 cut(s) 186
BstACI GRCGYC 1 cut(s) 293
BstAPI GCANNNNNTGC 1 cut(s) 563
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 6 cut(s) 169, 198, 220, 260, 559, 816
BstDEI CTNAG 5 cut(s) 204, 506, 511, 953, 1020
BstDSI CCRYGG 2 cut(s) 253, 807
BstF5I GGATG 3 cut(s) 892, 963, 1002
BstFNI CGCG 2 cut(s) 311, 714
BstH2I RGCGCY 1 cut(s) 642
BstHHI GCGC 2 cut(s) 313, 641
BstKTI GATC 5 cut(s) 132, 136, 249, 781, 840
BstMBI GATC 5 cut(s) 129, 133, 246, 778, 837
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstNSI RCATGY 2 cut(s) 447, 468
BstSCI CCNGG 3 cut(s) 566, 726, 909
BstSLI GKGCMC 2 cut(s) 593, 816
BstUI CGCG 2 cut(s) 311, 714
BstV1I GCAGC 4 cut(s) 45, 300, 672, 1003
Bsu15I ATCGAT 1 cut(s) 132
BsuI GTATCC 2 cut(s) 533, 930
BsuRI GGCC 2 cut(s) 91, 675
BsuTUI ATCGAT 1 cut(s) 132
BtgI CCRYGG 2 cut(s) 253, 807
BtsCI GGATG 3 cut(s) 892, 963, 1002
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 20, 192
Cac8I GCNNGC 6 cut(s) 169, 198, 220, 260, 559, 816
CfoI GCGC 2 cut(s) 313, 641
Cfr10I RCCGGY 2 cut(s) 167, 617
Cfr13I GGNCC 2 cut(s) 601, 631
ClaI ATCGAT 1 cut(s) 132
CseI GACGC 3 cut(s) 282, 703, 781
CspAI ACCGGT 1 cut(s) 617
CviAII CATG 7 cut(s) 228, 254, 444, 465, 536, 808, 980
DdeI CTNAG 5 cut(s) 204, 506, 511, 953, 1020
DpnI GATC 5 cut(s) 131, 135, 248, 780, 839
DpnII GATC 5 cut(s) 129, 133, 246, 778, 837
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 253, 807
Eco147I AGGCCT 2 cut(s) 91, 675
Eco47I GGWCC 2 cut(s) 601, 631
Eco47III AGCGCT 1 cut(s) 640
Eco57I CTGAAG 1 cut(s) 746
EcoO109I RGGNCCY 1 cut(s) 631
EcoT14I CCWWGG 2 cut(s) 253, 807
ErhI CCWWGG 2 cut(s) 253, 807
FaeI CATG 7 cut(s) 231, 257, 447, 468, 539, 811, 983
FaqI GGGAC 2 cut(s) 644, 710
FatI CATG 7 cut(s) 227, 253, 443, 464, 535, 807, 979
FbaI TGATCA 1 cut(s) 246
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 6 cut(s) 34, 201, 314, 317, 686, 1017
FokI GGATG 3 cut(s) 899, 970, 1009
Fsp4HI GCNGC 6 cut(s) 34, 201, 314, 317, 686, 1017
FspBI CTAG 2 cut(s) 20, 24
GlaI GCGC 2 cut(s) 312, 640
GluI GCNGC 6 cut(s) 34, 201, 314, 317, 686, 1017
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 1 cut(s) 341
HaeII RGCGCY 1 cut(s) 642
HaeIII GGCC 2 cut(s) 91, 675
HapII CCGG 6 cut(s) 168, 568, 599, 618, 728, 911
HgaI GACGC 3 cut(s) 282, 703, 781
HhaI GCGC 2 cut(s) 313, 641
Hin1I GRCGYC 1 cut(s) 293
Hin1II CATG 7 cut(s) 231, 257, 447, 468, 539, 811, 983
Hin6I GCGC 2 cut(s) 311, 639
HinP1I GCGC 2 cut(s) 311, 639
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 4 cut(s) 120, 550, 731, 949
HpaII CCGG 6 cut(s) 168, 568, 599, 618, 728, 911
HphI GGTGA 5 cut(s) 97, 256, 349, 764, 845
Hpy166II GTNNAC 3 cut(s) 623, 814, 898
Hpy188I TCNGA 6 cut(s) 79, 144, 268, 276, 697, 954
Hpy188III TCNNGA 4 cut(s) 547, 659, 863, 923
Hpy8I GTNNAC 3 cut(s) 623, 814, 898
Hpy99I CGWCG 3 cut(s) 298, 797, 902
HpyAV CCTTC 5 cut(s) 104, 110, 273, 328, 665
HpyCH4III ACNGT 3 cut(s) 179, 244, 649
HpyCH4IV ACGT 2 cut(s) 99, 747
HpyCH4V TGCA 4 cut(s) 4, 222, 535, 814
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpyF3I CTNAG 5 cut(s) 204, 506, 511, 953, 1020
HpySE526I ACGT 2 cut(s) 99, 747
Hsp92I GRCGYC 1 cut(s) 293
Hsp92II CATG 7 cut(s) 231, 257, 447, 468, 539, 811, 983
HspAI GCGC 2 cut(s) 311, 639
KflI GGGWCCC 1 cut(s) 631
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 5 cut(s) 129, 133, 246, 778, 837
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 4 cut(s) 41, 589, 701, 771
Lsp1109I GCAGC 4 cut(s) 45, 300, 672, 1003
LweI GCATC 1 cut(s) 808
MaeI CTAG 2 cut(s) 20, 24
MaeII ACGT 2 cut(s) 99, 747
MaeIII GTNAC 1 cut(s) 8
MalI GATC 5 cut(s) 131, 135, 248, 780, 839
MboI GATC 5 cut(s) 129, 133, 246, 778, 837
MhlI GDGCHC 2 cut(s) 593, 816
MluCI AATT 3 cut(s) 282, 890, 967
MmeI TCCRAC 1 cut(s) 864
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 305
MseI TTAA 2 cut(s) 41, 963
MslI CAYNNNNRTG 2 cut(s) 375, 872
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 6 cut(s) 168, 568, 599, 618, 728, 911
MspR9I CCNGG 3 cut(s) 568, 728, 911
MvnI CGCG 2 cut(s) 311, 714
MwoI GCNNNNNNNGC 1 cut(s) 563
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 3 cut(s) 568, 728, 911
NcoI CCATGG 2 cut(s) 253, 807
NdeII GATC 5 cut(s) 129, 133, 246, 778, 837
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 7 cut(s) 231, 257, 447, 468, 539, 811, 983
NlaIV GGNNCC 3 cut(s) 616, 632, 633
NspI RCATGY 2 cut(s) 447, 468
PceI AGGCCT 2 cut(s) 91, 675
PciI ACATGT 1 cut(s) 443
PciSI GCTCTTC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 792
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 4 cut(s) 120, 550, 731, 949
PfoI TCCNGGA 1 cut(s) 726
PinAI ACCGGT 1 cut(s) 617
PkrI GCNGC 6 cut(s) 35, 202, 315, 318, 687, 1018
Ppu21I YACGTR 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 631
PscI ACATGT 1 cut(s) 443
Psp1406I AACGTT 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 635
PspN4I GGNNCC 3 cut(s) 616, 632, 633
PspPI GGNCC 2 cut(s) 601, 631
PspPPI RGGWCCY 1 cut(s) 631
RseI CAYNNNNRTG 2 cut(s) 375, 872
SalI GTCGAC 1 cut(s) 896
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 41, 963
SatI GCNGC 6 cut(s) 34, 201, 314, 317, 686, 1017
Sau3AI GATC 5 cut(s) 129, 133, 246, 778, 837
Sau96I GGNCC 2 cut(s) 601, 631
ScrFI CCNGG 3 cut(s) 568, 728, 911
SduI GDGCHC 2 cut(s) 593, 816
SfaNI GCATC 1 cut(s) 808
SgrAI CRCCGGYG 1 cut(s) 617
SinI GGWCC 2 cut(s) 601, 631
SmiMI CAYNNNNRTG 2 cut(s) 375, 872
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
Sse9I AATT 3 cut(s) 282, 890, 967
SseBI AGGCCT 2 cut(s) 91, 675
SsiI CCGC 3 cut(s) 201, 317, 994
SspMI CTAG 2 cut(s) 20, 24
StuI AGGCCT 2 cut(s) 91, 675
StyD4I CCNGG 3 cut(s) 566, 726, 909
StyI CCWWGG 2 cut(s) 253, 807
TaaI ACNGT 3 cut(s) 179, 244, 649
TaiI ACGT 2 cut(s) 102, 750
TaqI TCGA 9 cut(s) 132, 296, 460, 546, 678, 795, 802, 836, 897
TasI AATT 3 cut(s) 282, 890, 967
TauI GCSGC 2 cut(s) 203, 319
TfiI GAWTC 4 cut(s) 120, 550, 731, 949
Tru1I TTAA 2 cut(s) 41, 963
Tru9I TTAA 2 cut(s) 41, 963
TscAI CASTG 2 cut(s) 20, 192
TseI GCWGC 4 cut(s) 33, 313, 685, 1016
TspDTI ATGAA 3 cut(s) 359, 890, 903
TspGWI ACGGA 3 cut(s) 576, 714, 839
TspRI CASTG 2 cut(s) 20, 192
VneI GTGCAC 1 cut(s) 812
VpaK11BI GGWCC 2 cut(s) 601, 631
XceI RCATGY 2 cut(s) 447, 468
XmiI GTMKAC 1 cut(s) 897
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 2 cut(s) 20, 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.