Prupe.1G499300_v2.0.a1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0022391
Physical Location & Seq
Forward (+)
41342700 .. 41345562
2863 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G499300.2

Sequence Viewer

Length: 1137 bp
ATGCAACTATATATACACGTGCCATGGCACCAATGCTTAACGATCCAAAGCTATAAACCATCAACATATACCATGGGAGAAGTCAACCCATCATACATCCTTTCAGAAGAACACAGGCCCAAACACGTCATCACTGAAGGTGAAGGAATCCCATTGATCGATCTCTCTCCAATAACCAACAGGGAAGGGTTATCAGCTGATCCCAATAAGGCAGTGGAAGACCTTGCTGCTAAGATAGGTGAGGCTTGCAGGACTTGGGGGTTCTTCAGTGTGATCAACCATGGCGTGCCTTTGGGCATCCGGAGAAGAATCATGGAGGTGGCACGGAAATTTTTCGCATTGCCGGTGGAGGAGAAGAAGAAAGTGAGCAGAGAGGACCATAACACTGCAGGGTTTCATAACGATGAGCATAGCAAAGACTTTAAGGATTGGAAAGAGGTTTATGATTTTTATGTTAATGATGGGATGCTAATGCCGGCTTCCCATGAACTTGATGACCCTGAAATTGTTCCGTGGTTCACTCCATGGCCCGAGAACCTACCCGAGTTCAGGGAGACATGCGAAGAATACGGTCGAGCATGTGAGAAGCTATTCTTCAATTTGTTGGAACTTGTCAGCCTCAGCTTAGGCTTACCCCCGGAGAGGCTGCATGGGTACTTTGAGAATCAAGCAAGCTTTGCCCGACTCAATTACTACCCTCCATGTCCCAAACCGGAACTTGTTCTTGGCACCGGTGGACACAAGGACCCTAGTGCTCTAACGGTCCTCGCTCAAGAAGATGTCGAAGGCCTCGACGTGCTTCGAAAATCAGACGGAGCATGGGTTCGTGTCAAGCCTGTCCCGGATTCCTTTGTCATCAATGTTGGCGATGTTCTTCAGGTATGGAGCAATGACCTTTACGAAAGTGTCGAGCATCGGGCCGAGGTGAATGCAGAAACAGAAAGGTATTCCATCCCTATATTTTTTCACCCTTCTCATGATGTAACCATGAAGCCGTTGGATGAGTTGGTTGATGAACAAAGCCCGGCCAAGTACCCCGAATACAAGGCAGGGAAGTGGCTGAATTTGAGGATGTATAATAACTACAAGAAACATGGCTTCTATATGCGGATGACTGACTACAAGGTTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

379

Amino Acids

43.53

Weight (kDa)

5.36

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022391)

Species Orthologous Gene IDs
malus_domestica MD08G1163300.v1.1 MD08G1163600.v1.1
prunus_persica Prupe.1G499300_v2.0.a1 Prupe.1G499300_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 27, 728
AccIII TCCGGA 1 cut(s) 300
AciI CCGC 1 cut(s) 1108
AclWI GGATC 2 cut(s) 37, 194
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 2 cut(s) 329, 1063
AcuI CTGAAG 3 cut(s) 156, 250, 860
AcvI CACGTG 1 cut(s) 19
AfaI GTAC 2 cut(s) 656, 1034
AfiI CCNNNNNNNGG 2 cut(s) 549, 642
AflIII ACRYGT 2 cut(s) 16, 124
AgeI ACCGGT 1 cut(s) 731
AgsI TTSAA 1 cut(s) 598
AjiI CACGTC 2 cut(s) 127, 796
AjuI GAANNNNNNNTTGG 2 cut(s) 708, 740
AloI GAACNNNNNNTCC 2 cut(s) 807, 839
AluBI AGCT 5 cut(s) 51, 197, 589, 624, 675
AluI AGCT 5 cut(s) 51, 197, 589, 624, 675
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 2 cut(s) 37, 194
Ama87I CYCGRG 2 cut(s) 530, 542
Aor13HI TCCGGA 1 cut(s) 300
AoxI GGCC 5 cut(s) 116, 527, 787, 918, 1026
ApeKI GCWGC 3 cut(s) 227, 646, 1130
ApoI RAATTY 2 cut(s) 329, 1063
AsiGI ACCGGT 1 cut(s) 731
Asp700I GAANNNNTTC 4 cut(s) 332, 507, 590, 720
AspS9I GGNCC 6 cut(s) 117, 376, 528, 745, 763, 918
AsuC2I CCSGG 3 cut(s) 638, 842, 1025
AsuHPI GGTGA 4 cut(s) 152, 251, 937, 959
AsuII TTCGAA 1 cut(s) 802
AvaI CYCGRG 2 cut(s) 530, 542
AvaII GGWCC 3 cut(s) 376, 745, 763
BanI GGYRCC 2 cut(s) 27, 728
BbrPI CACGTG 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 757
BbvCI CCTCAGC 1 cut(s) 620
BbvI GCAGC 3 cut(s) 214, 633, 1117
BccI CCATC 4 cut(s) 67, 97, 455, 959
BceAI ACGGC 1 cut(s) 979
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 3 cut(s) 638, 842, 1025
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 1 cut(s) 750
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 3 cut(s) 228, 647, 1131
BlsI GCNGC 3 cut(s) 229, 648, 1132
Bme1390I CCNGG 3 cut(s) 638, 842, 1025
Bme18I GGWCC 3 cut(s) 376, 745, 763
BmeT110I CYCGRG 2 cut(s) 530, 542
BmgBI CACGTC 2 cut(s) 127, 796
BmgT120I GGNCC 6 cut(s) 117, 376, 528, 745, 763, 918
BmiI GGNNCC 3 cut(s) 29, 730, 747
BmrFI CCNGG 3 cut(s) 638, 842, 1025
BmsI GCATC 3 cut(s) 306, 456, 922
BpiI GAAGAC 1 cut(s) 225
Bpu10I CCTNAGC 2 cut(s) 620, 625
Bpu14I TTCGAA 1 cut(s) 802
BpuEI CTTGAG 1 cut(s) 756
BpuMI CCSGG 3 cut(s) 638, 842, 1025
Bsa29I ATCGAT 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 19
BsaJI CCNNGG 7 cut(s) 23, 72, 280, 512, 524, 636, 921
BsaWI WCCGGW 3 cut(s) 300, 712, 731
BsaXI ACNNNNNCTCC 2 cut(s) 807, 837
Bsc4I CCNNNNNNNGG 2 cut(s) 549, 642
Bse118I RCCGGY 3 cut(s) 343, 475, 731
Bse3DI GCAATG 2 cut(s) 338, 895
BseAI TCCGGA 1 cut(s) 300
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 7 cut(s) 23, 72, 280, 512, 524, 636, 921
BseGI GGATG 7 cut(s) 96, 297, 471, 951, 1006, 1077, 1116
BseLI CCNNNNNNNGG 2 cut(s) 549, 642
BseMI GCAATG 2 cut(s) 338, 895
BseMII CTCAG 1 cut(s) 634
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 3 cut(s) 214, 633, 1117
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 5 cut(s) 118, 529, 789, 920, 1028
BshNI GGYRCC 2 cut(s) 27, 728
BshTI ACCGGT 1 cut(s) 731
BshVI ATCGAT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 757
BsiHKCI CYCGRG 2 cut(s) 530, 542
BsiSI CCGG 8 cut(s) 301, 344, 476, 638, 713, 732, 842, 1025
BslFI GGGAC 2 cut(s) 690, 824
BslI CCNNNNNNNGG 2 cut(s) 549, 642
BsmAI GTCTC 1 cut(s) 548
BsmFI GGGAC 2 cut(s) 690, 824
BsmI GAATGC 1 cut(s) 934
BsnI GGCC 5 cut(s) 118, 529, 789, 920, 1028
BsoBI CYCGRG 2 cut(s) 530, 542
Bsp119I TTCGAA 1 cut(s) 802
Bsp1286I GDGCHC 1 cut(s) 757
Bsp13I TCCGGA 1 cut(s) 300
Bsp143I GATC 5 cut(s) 42, 156, 160, 199, 273
Bsp19I CCATGG 4 cut(s) 23, 72, 280, 524
BspACI CCGC 1 cut(s) 1108
BspANI GGCC 5 cut(s) 118, 529, 789, 920, 1028
BspCNI CTCAG 1 cut(s) 633
BspDI ATCGAT 1 cut(s) 159
BspEI TCCGGA 1 cut(s) 300
BspHI TCATGA 1 cut(s) 976
BspLI GGNNCC 3 cut(s) 29, 730, 747
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 2 cut(s) 37, 194
BspT104I TTCGAA 1 cut(s) 802
BspT107I GGYRCC 2 cut(s) 27, 728
BsrDI GCAATG 2 cut(s) 338, 895
BsrFI RCCGGY 3 cut(s) 343, 475, 731
BssAI RCCGGY 3 cut(s) 343, 475, 731
BssECI CCNNGG 7 cut(s) 23, 72, 280, 512, 524, 636, 921
BssMI GATC 5 cut(s) 42, 156, 160, 199, 273
BssT1I CCWWGG 4 cut(s) 23, 72, 280, 524
Bst4CI ACNGT 2 cut(s) 572, 763
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBAI YACGTR 1 cut(s) 19
BstBI TTCGAA 1 cut(s) 802
BstC8I GCNNGC 4 cut(s) 247, 287, 477, 673
BstDEI CTNAG 4 cut(s) 231, 620, 625, 1134
BstDSI CCRYGG 5 cut(s) 23, 72, 280, 512, 524
BstF5I GGATG 7 cut(s) 96, 297, 471, 951, 1006, 1077, 1116
BstKTI GATC 5 cut(s) 45, 159, 163, 202, 276
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 5 cut(s) 42, 156, 160, 199, 273
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 1 cut(s) 677
BstNSI RCATGY 2 cut(s) 561, 582
BstSCI CCNGG 3 cut(s) 636, 840, 1023
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 3 cut(s) 214, 633, 1117
BstV2I GAAGAC 1 cut(s) 225
Bsu15I ATCGAT 1 cut(s) 159
BsuRI GGCC 5 cut(s) 118, 529, 789, 920, 1028
BsuTUI ATCGAT 1 cut(s) 159
BtgI CCRYGG 5 cut(s) 23, 72, 280, 512, 524
BtgZI GCGATG 1 cut(s) 882
BtrI CACGTC 2 cut(s) 127, 796
BtsCI GGATG 7 cut(s) 96, 297, 471, 951, 1006, 1077, 1116
BtsI GCAGTG 2 cut(s) 219, 384
BtsIMutI CAGTG 4 cut(s) 132, 219, 274, 384
Cac8I GCNNGC 4 cut(s) 247, 287, 477, 673
CciI TCATGA 1 cut(s) 976
Cfr10I RCCGGY 3 cut(s) 343, 475, 731
Cfr13I GGNCC 6 cut(s) 117, 376, 528, 745, 763, 918
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 2 cut(s) 655, 1033
CspAI ACCGGT 1 cut(s) 731
CviQI GTAC 2 cut(s) 655, 1033
DdeI CTNAG 4 cut(s) 231, 620, 625, 1134
DpnI GATC 5 cut(s) 44, 158, 162, 201, 275
DpnII GATC 5 cut(s) 42, 156, 160, 199, 273
EaeI YGGCCR 1 cut(s) 1026
Eco130I CCWWGG 4 cut(s) 23, 72, 280, 524
Eco147I AGGCCT 1 cut(s) 789
Eco47I GGWCC 3 cut(s) 376, 745, 763
Eco57I CTGAAG 3 cut(s) 156, 250, 860
Eco72I CACGTG 1 cut(s) 19
Eco88I CYCGRG 2 cut(s) 530, 542
EcoO109I RGGNCCY 1 cut(s) 745
EcoT14I CCWWGG 4 cut(s) 23, 72, 280, 524
ErhI CCWWGG 4 cut(s) 23, 72, 280, 524
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 2 cut(s) 690, 824
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 3 cut(s) 228, 647, 1131
FokI GGATG 7 cut(s) 83, 284, 478, 938, 1013, 1084, 1123
Fsp4HI GCNGC 3 cut(s) 228, 647, 1131
FspBI CTAG 1 cut(s) 750
GluI GCNGC 3 cut(s) 228, 647, 1131
HaeIII GGCC 5 cut(s) 118, 529, 789, 920, 1028
HapII CCGG 8 cut(s) 301, 344, 476, 638, 713, 732, 842, 1025
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HindIII AAGCTT 1 cut(s) 673
HinfI GANTC 5 cut(s) 147, 309, 664, 684, 845
HpaII CCGG 8 cut(s) 301, 344, 476, 638, 713, 732, 842, 1025
HphI GGTGA 4 cut(s) 152, 251, 937, 959
Hpy166II GTNNAC 3 cut(s) 85, 519, 737
Hpy188I TCNGA 2 cut(s) 106, 811
Hpy188III TCNNGA 3 cut(s) 301, 773, 977
Hpy8I GTNNAC 3 cut(s) 85, 519, 737
Hpy99I CGWCG 1 cut(s) 797
HpyAV CCTTC 5 cut(s) 131, 137, 179, 779, 981
HpyCH4III ACNGT 2 cut(s) 572, 763
HpyCH4IV ACGT 3 cut(s) 18, 126, 795
HpyCH4V TGCA 5 cut(s) 4, 249, 389, 649, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 677
HpyF3I CTNAG 4 cut(s) 231, 620, 625, 1134
HpySE526I ACGT 3 cut(s) 18, 126, 795
Kpn2I TCCGGA 1 cut(s) 300
KroI GCCGGC 1 cut(s) 475
KroNI GCCGGC 1 cut(s) 477
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 5 cut(s) 42, 156, 160, 199, 273
LmnI GCTCC 2 cut(s) 815, 885
Lsp1109I GCAGC 3 cut(s) 214, 633, 1117
LweI GCATC 3 cut(s) 306, 456, 922
MaeI CTAG 1 cut(s) 750
MaeII ACGT 3 cut(s) 18, 126, 795
MaeIII GTNAC 1 cut(s) 982
MalI GATC 5 cut(s) 44, 158, 162, 201, 275
MboI GATC 5 cut(s) 42, 156, 160, 199, 273
MhlI GDGCHC 1 cut(s) 757
MluCI AATT 5 cut(s) 329, 504, 598, 688, 1063
MlyI GAGTC 1 cut(s) 678
MmeI TCCRAC 2 cut(s) 585, 978
MroI TCCGGA 1 cut(s) 300
MroNI GCCGGC 1 cut(s) 475
MroXI GAANNNNTTC 4 cut(s) 332, 507, 590, 720
MseI TTAA 3 cut(s) 38, 423, 456
MslI CAYNNNNRTG 2 cut(s) 317, 402
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 8 cut(s) 301, 344, 476, 638, 713, 732, 842, 1025
MspR9I CCNGG 3 cut(s) 638, 842, 1025
Mva1269I GAATGC 1 cut(s) 934
MwoI GCNNNNNNNGC 1 cut(s) 677
NaeI GCCGGC 1 cut(s) 477
NciI CCSGG 3 cut(s) 638, 842, 1025
NcoI CCATGG 4 cut(s) 23, 72, 280, 524
NdeII GATC 5 cut(s) 42, 156, 160, 199, 273
NgoMIV GCCGGC 1 cut(s) 475
NlaIV GGNNCC 3 cut(s) 29, 730, 747
NmeAIII GCCGAG 1 cut(s) 946
NspI RCATGY 2 cut(s) 561, 582
NspV TTCGAA 1 cut(s) 802
PagI TCATGA 1 cut(s) 976
PceI AGGCCT 1 cut(s) 789
PcsI WCGNNNNNNNCGW 2 cut(s) 789, 906
PctI GAATGC 1 cut(s) 934
PdiI GCCGGC 1 cut(s) 477
PdmI GAANNNNTTC 4 cut(s) 332, 507, 590, 720
PfeI GAWTC 4 cut(s) 147, 309, 664, 845
PfoI TCCNGGA 1 cut(s) 840
PinAI ACCGGT 1 cut(s) 731
PkrI GCNGC 3 cut(s) 229, 648, 1132
PleI GAGTC 1 cut(s) 678
PmaCI CACGTG 1 cut(s) 19
PmlI CACGTG 1 cut(s) 19
PpsI GAGTC 1 cut(s) 678
Ppu21I YACGTR 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 745
Psp5II RGGWCCY 1 cut(s) 745
PspCI CACGTG 1 cut(s) 19
PspN4I GGNNCC 3 cut(s) 29, 730, 747
PspPI GGNCC 6 cut(s) 117, 376, 528, 745, 763, 918
PspPPI RGGWCCY 1 cut(s) 745
PstI CTGCAG 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 2 cut(s) 656, 1034
RsaNI GTAC 2 cut(s) 655, 1033
RseI CAYNNNNRTG 2 cut(s) 317, 402
SaqAI TTAA 3 cut(s) 38, 423, 456
SatI GCNGC 3 cut(s) 228, 647, 1131
Sau3AI GATC 5 cut(s) 42, 156, 160, 199, 273
Sau96I GGNCC 6 cut(s) 117, 376, 528, 745, 763, 918
SchI GAGTC 1 cut(s) 678
ScrFI CCNGG 3 cut(s) 638, 842, 1025
SduI GDGCHC 1 cut(s) 757
SfaNI GCATC 3 cut(s) 306, 456, 922
SfcI CTRYAG 1 cut(s) 387
SfuI TTCGAA 1 cut(s) 802
SgrAI CRCCGGYG 1 cut(s) 731
SinI GGWCC 3 cut(s) 376, 745, 763
SmiMI CAYNNNNRTG 2 cut(s) 317, 402
SmlI CTYRAG 1 cut(s) 771
SmoI CTYRAG 1 cut(s) 771
Sse9I AATT 5 cut(s) 329, 504, 598, 688, 1063
SseBI AGGCCT 1 cut(s) 789
SsiI CCGC 1 cut(s) 1108
SspMI CTAG 1 cut(s) 750
StuI AGGCCT 1 cut(s) 789
StyD4I CCNGG 3 cut(s) 636, 840, 1023
StyI CCWWGG 4 cut(s) 23, 72, 280, 524
TaaI ACNGT 2 cut(s) 572, 763
TaiI ACGT 3 cut(s) 21, 129, 798
TaqI TCGA 6 cut(s) 159, 574, 783, 792, 802, 909
TasI AATT 5 cut(s) 329, 504, 598, 688, 1063
TfiI GAWTC 4 cut(s) 147, 309, 664, 845
Tru1I TTAA 3 cut(s) 38, 423, 456
Tru9I TTAA 3 cut(s) 38, 423, 456
TscAI CASTG 4 cut(s) 139, 219, 274, 391
TseI GCWGC 3 cut(s) 227, 646, 1130
TspDTI ATGAA 4 cut(s) 386, 501, 1004, 1029
TspGWI ACGGA 3 cut(s) 340, 501, 828
TspRI CASTG 4 cut(s) 139, 219, 274, 391
VpaK11BI GGWCC 3 cut(s) 376, 745, 763
XapI RAATTY 2 cut(s) 329, 1063
XceI RCATGY 2 cut(s) 561, 582
XcmI CCANNNNNNNNNTGG 2 cut(s) 211, 994
XmnI GAANNNNTTC 4 cut(s) 332, 507, 590, 720
XspI CTAG 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.