MD08G1221900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006263
Physical Location & Seq
Forward (+)
28445567 .. 28449963
4397 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1221900.v1.1.491

Sequence Viewer

Length: 1923 bp
ATGGATTTGAACGAGAAGAGTATACTATTTTCTCATGATGGTCAGTTCACAAAAAATGACCACTTTGGTGATACTGCTCTATGCTTGAATAGCCCTGGCTCCGGTGGAAGCAACACACCCAGGTATAGGTGTAGACAAAACAATTTTAGGGTCAACTGTTCCAGTGCTCCCGATGACGGCTGTAAGTTGGTACTTGGATTGGGCCCGACACCAAGTGCATACTGCGATGATTATTACAATTTTGGACCCACAAAGAATAGAGGATCAACCACTGCATTATCTCAGGGATTTGCTTTTGACGGTGATTCAATCCTTCAACTCGGTCTTTCTGGTGGGACTCTTGAAGCTTCAACTGTGCTTGATTTTTCAATTTCGGGAGAGACTGATGTTAATATTACTCGTGTATCTTCTGAAGATAATCAACTCCCAATTCCACTTGTTGATGAGGGTTCTACTTCTTCCAGGAAATCAGGTGGTTATATGCCATCACTTCTTTTTGCTCCTAGGAGTGACAGTGTCAATCTTTCTCTGCACAGTAAAGAACTTTTAAACCTCGGGGACAAATGCCAGCTAACTGAGCTAAGGCATGAACCTTCTGTTACAACAGATTACTCAACAGAATCAATATCTGAGCAGACAACTACAGGGGCATCTTCAGACAACCGAATTAGCAGTCTAAAAAAATGCAAATTTTTTGGCTGTAGAAAGGGAGCACGAGGGGCATCAGGTCTTTGTATTGGTCATGGGGGCGGACAGAGATGCCAAAAACCTGGGTGCAAGAAGGGTGCTGAGAGCCGAACGGCGTACTGCAAGGCACATGGTGGAGGGAAGAGGTGTCAACACTTGGGGTGCACTAAAAGTGCTGAGGGGAAAACAGAGTACTGCATAGCTCATGGTGGTGGCAAAAGATGTAGTTATCCAGGTGGATGTACCAAGGCTGCACGAGGAAAATCAGGACTTTGTATCAGACATGGGGGAGGGAAAAGGTGTAAGGTGGATGGCTGCACTCGTAGTGCTGAAGGACAGGCTGGTTTGTGCATCTCGCATGGAGGTGGTCGCCGTTGCCAGCACGAGGCGTGTGCAAAGGGAGCACAAGGGAGCACTATGTACTGCAAGGCACATGGTGGTGGAAAGCGTTGCATATTTCAAGGGTGCACCAAGGGTGCCGAAGGAAGCACACCACTCTGCAAGGGACATGGTGGGGGGAAGCGCTGTCTCTTTGATGGTGGTGGGATTTGCCCTAAGAGCGTACATGGAGGCACTAATTTCTGTGTTGCTCATGGTGGCGGAAAGAGGTGTTCTGTGCCAGGCTGCACAAGAAGTGCCCGTGGCCGCACTGATTGTTGTGTTAGGCATGGTGGAGGTAAGCGTTGTAAGTTTGATAACTGTGGAAAAAGTGCCCAAGGGAGCACAGACTTCTGCAAGGCCCATGGTGGGGGAAAGCGATGCACTTGGGGAGAGGGTAAATGTGAAAAATTCGCCAGGGGCAAGAGTGGTTTATGTGCTGCTCATAGCAGTATGGTTCAGGACCGGGGGATAGATAAGGCAGGTCTCATTGGACCAGGACTTTTCCATGGGCTTGTATCTGCGGCTTCAACTGCAGGGAGCAGCTTTGACAACAATCATTCTTCTTCAGGAACCAGTGCCATTTCTGACAGCATGGATTCACTGGAAAAGCCCGAAAAAACACATCTCATACCCTCGCAGGTATTGGTTCCTCTATCGATGAAGTCTTCATCATCCTATTCACAGTTCTCGAGTTCTGAGAAGCCTGAGGAGGGGAGAGACGGGTATGGCGTCGGTGTTGGCAGTTGTAGTGGGATAAAGAGCTTGGAGTTCAAAATCCCGGAAGGGAGAGTCCACGGAGGACCTCTCATGTCGCTATTTGGTGGAGATCTGAAAAATGCTATCGATGGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

641

Amino Acids

66.55

Weight (kDa)

8.98

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zf_WRKY19 PF24906 251 - 275 4.9e-06 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 276 - 300 7.8e-09 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 301 - 326 4.1e-06 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 327 - 351 4.6e-10 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 352 - 376 2.6e-06 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 430 - 454 4.5e-08 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 455 - 479 1.5e-07 WRKY19 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1538, 1696
AccB1I GGYRCC 1 cut(s) 1163
AccI GTMKAC 2 cut(s) 22, 133
AciI CCGC 4 cut(s) 750, 1287, 1333, 1589
AclWI GGATC 1 cut(s) 271
AcoI YGGCCR 1 cut(s) 1330
AcsI RAATTY 2 cut(s) 689, 1475
AcuI CTGAAG 4 cut(s) 432, 639, 1038, 1617
AcyI GRCGYC 1 cut(s) 1797
AdeI CACNNNGTG 3 cut(s) 215, 821, 1124
AfaI GTAC 6 cut(s) 192, 806, 881, 931, 1109, 1251
AfeI AGCGCT 1 cut(s) 1211
AfiI CCNNNNNNNGG 7 cut(s) 101, 126, 176, 1072, 1434, 1435, 1778
AjnI CCWGG 8 cut(s) 94, 119, 461, 769, 919, 1306, 1481, 1561
AleI CACNNNNGTG 1 cut(s) 66
AluBI AGCT 6 cut(s) 347, 571, 580, 890, 1611, 1830
AluI AGCT 6 cut(s) 347, 571, 580, 890, 1611, 1830
Alw21I GWGCWC 7 cut(s) 169, 715, 854, 1093, 1103, 1157, 1412
Alw26I GTCTC 4 cut(s) 374, 1220, 1556, 1779
Alw44I GTGCAC 2 cut(s) 850, 1153
AlwI GGATC 1 cut(s) 271
Ama87I CYCGRG 2 cut(s) 554, 1756
Aor51HI AGCGCT 1 cut(s) 1211
AoxI GGCC 3 cut(s) 202, 1330, 1425
ApaI GGGCCC 1 cut(s) 206
ApaLI GTGCAC 2 cut(s) 850, 1153
ApeKI GCWGC 5 cut(s) 938, 1002, 1311, 1505, 1608
ApoI RAATTY 2 cut(s) 689, 1475
ArsI GACNNNNNNTTYG 2 cut(s) 658, 690
AspA2I CCTAGG 1 cut(s) 503
AspLEI GCGC 1 cut(s) 1212
AspS9I GGNCC 7 cut(s) 202, 203, 245, 1426, 1528, 1559, 1868
AsuC2I CCSGG 2 cut(s) 1532, 1847
AsuHPI GGTGA 2 cut(s) 80, 314
AvaI CYCGRG 2 cut(s) 554, 1756
AvaII GGWCC 4 cut(s) 245, 1528, 1559, 1868
AvrII CCTAGG 1 cut(s) 503
AxyI CCTNAGG 1 cut(s) 1773
BaeGI GKGCMC 5 cut(s) 206, 854, 1157, 1327, 1402
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BanI GGYRCC 1 cut(s) 1163
BanII GRGCYC 1 cut(s) 206
BauI CACGAG 4 cut(s) 399, 714, 942, 1070
BbsI GAAGAC 1 cut(s) 1725
Bbv12I GWGCWC 7 cut(s) 169, 715, 854, 1093, 1103, 1157, 1412
BbvCI CCTCAGC 1 cut(s) 864
BbvI GCAGC 5 cut(s) 925, 989, 1298, 1492, 1620
BccI CCATC 5 cut(s) 32, 493, 992, 1217, 1907
BceAI ACGGC 3 cut(s) 193, 816, 1044
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BciT130I CCWGG 8 cut(s) 96, 121, 463, 771, 921, 1308, 1483, 1563
BcnI CCSGG 2 cut(s) 1532, 1847
BcoDI GTCTC 4 cut(s) 374, 1220, 1556, 1779
BfaI CTAG 1 cut(s) 504
BfmI CTRYAG 3 cut(s) 642, 700, 1599
BfoI RGCGCY 1 cut(s) 1213
BfuAI ACCTGC 2 cut(s) 1538, 1696
BglII AGATCT 1 cut(s) 1894
BisI GCNGC 7 cut(s) 939, 1003, 1312, 1333, 1506, 1590, 1609
BlnI CCTAGG 1 cut(s) 503
BlsI GCNGC 7 cut(s) 940, 1004, 1313, 1334, 1507, 1591, 1610
BmcAI AGTACT 1 cut(s) 881
Bme18I GGWCC 4 cut(s) 245, 1528, 1559, 1868
BmeT110I CYCGRG 2 cut(s) 554, 1756
BmgT120I GGNCC 7 cut(s) 202, 203, 245, 1426, 1528, 1559, 1868
BmiI GGNNCC 6 cut(s) 100, 204, 247, 1165, 1639, 1716
BmsI GCATC 5 cut(s) 659, 731, 749, 1047, 1436
BpiI GAAGAC 1 cut(s) 1725
BplI GAGNNNNNCTC 2 cut(s) 1857, 1889
Bpu10I CCTNAGC 2 cut(s) 581, 864
BpuMI CCSGG 2 cut(s) 1532, 1847
Bsa29I ATCGAT 2 cut(s) 1724, 1911
BsaHI GRCGYC 1 cut(s) 1797
BsaI GGTCTC 1 cut(s) 1556
BsaWI WCCGGW 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 7 cut(s) 101, 126, 176, 1072, 1434, 1435, 1778
Bse1I ACTGG 3 cut(s) 162, 1641, 1674
Bse21I CCTNAGG 1 cut(s) 1773
BseBI CCWGG 8 cut(s) 96, 121, 463, 771, 921, 1308, 1483, 1563
BseCI ATCGAT 2 cut(s) 1724, 1911
BseGI GGATG 3 cut(s) 932, 1003, 1739
BseLI CCNNNNNNNGG 7 cut(s) 101, 126, 176, 1072, 1434, 1435, 1778
BseMII CTCAG 7 cut(s) 296, 567, 621, 780, 855, 1755, 1764
BseNI ACTGG 3 cut(s) 162, 1641, 1674
BseRI GAGGAG 1 cut(s) 1790
BseSI GKGCMC 5 cut(s) 206, 854, 1157, 1327, 1402
BseXI GCAGC 5 cut(s) 925, 989, 1298, 1492, 1620
BsgI GTGCAG 4 cut(s) 515, 924, 988, 1297
BshFI GGCC 3 cut(s) 204, 1332, 1427
BshNI GGYRCC 1 cut(s) 1163
BshVI ATCGAT 2 cut(s) 1724, 1911
BsiHKAI GWGCWC 7 cut(s) 169, 715, 854, 1093, 1103, 1157, 1412
BsiHKCI CYCGRG 2 cut(s) 554, 1756
BsiSI CCGG 3 cut(s) 102, 1531, 1847
BslFI GGGAC 3 cut(s) 349, 572, 1206
BslI CCNNNNNNNGG 7 cut(s) 101, 126, 176, 1072, 1434, 1435, 1778
BsmAI GTCTC 4 cut(s) 374, 1220, 1556, 1779
BsmBI CGTCTC 1 cut(s) 1779
BsmFI GGGAC 3 cut(s) 349, 572, 1206
BsnI GGCC 3 cut(s) 204, 1332, 1427
Bso31I GGTCTC 1 cut(s) 1556
BsoBI CYCGRG 2 cut(s) 554, 1756
Bsp120I GGGCCC 1 cut(s) 202
Bsp143I GATC 2 cut(s) 263, 1894
Bsp19I CCATGG 2 cut(s) 1429, 1573
BspACI CCGC 4 cut(s) 750, 1287, 1333, 1589
BspANI GGCC 3 cut(s) 204, 1332, 1427
BspCNI CTCAG 7 cut(s) 295, 568, 622, 781, 856, 1756, 1765
BspDI ATCGAT 2 cut(s) 1724, 1911
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 6 cut(s) 100, 204, 247, 1165, 1639, 1716
BspMAI CTGCAG 1 cut(s) 1603
BspMI ACCTGC 2 cut(s) 1538, 1696
BspPI GGATC 1 cut(s) 271
BspT107I GGYRCC 1 cut(s) 1163
BspTNI GGTCTC 1 cut(s) 1556
BsrI ACTGG 3 cut(s) 162, 1641, 1674
BssMI GATC 2 cut(s) 263, 1894
BssNAI GTATAC 1 cut(s) 23
BssNI GRCGYC 1 cut(s) 1797
BssSI CACGAG 4 cut(s) 399, 714, 942, 1070
BssT1I CCWWGG 6 cut(s) 503, 933, 1158, 1402, 1429, 1573
Bst1107I GTATAC 1 cut(s) 23
Bst2BI CACGAG 4 cut(s) 399, 714, 942, 1070
Bst2UI CCWGG 8 cut(s) 96, 121, 463, 771, 921, 1308, 1483, 1563
Bst4CI ACNGT 7 cut(s) 158, 302, 355, 515, 536, 1388, 1752
Bst6I CTCTTC 2 cut(s) 11, 824
BstACI GRCGYC 1 cut(s) 1797
BstC8I GCNNGC 2 cut(s) 569, 1067
BstDEI CTNAG 9 cut(s) 282, 576, 581, 630, 789, 864, 1242, 1764, 1773
BstDSI CCRYGG 4 cut(s) 1327, 1429, 1573, 1861
BstF5I GGATG 3 cut(s) 932, 1003, 1739
BstH2I RGCGCY 1 cut(s) 1213
BstHHI GCGC 1 cut(s) 1212
BstKTI GATC 2 cut(s) 266, 1897
BstMAI GTCTC 4 cut(s) 374, 1220, 1556, 1779
BstMBI GATC 2 cut(s) 263, 1894
BstMWI GCNNNNNNNGC 6 cut(s) 90, 577, 719, 1088, 1245, 1598
BstNI CCWGG 8 cut(s) 96, 121, 463, 771, 921, 1308, 1483, 1563
BstSFI CTRYAG 3 cut(s) 642, 700, 1599
BstSLI GKGCMC 5 cut(s) 206, 854, 1157, 1327, 1402
BstV1I GCAGC 5 cut(s) 925, 989, 1298, 1492, 1620
BstV2I GAAGAC 1 cut(s) 1725
BstX2I RGATCY 1 cut(s) 1894
BstXI CCANNNNNNTGG 1 cut(s) 770
BstYI RGATCY 1 cut(s) 1894
BstZ17I GTATAC 1 cut(s) 23
Bsu15I ATCGAT 2 cut(s) 1724, 1911
Bsu36I CCTNAGG 1 cut(s) 1773
BsuRI GGCC 3 cut(s) 204, 1332, 1427
BsuTUI ATCGAT 2 cut(s) 1724, 1911
BtgI CCRYGG 4 cut(s) 1327, 1429, 1573, 1861
BtgZI GCGATG 2 cut(s) 240, 1459
BtsCI GGATG 3 cut(s) 932, 1003, 1739
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 6 cut(s) 169, 270, 520, 1335, 1648, 1667
BveI ACCTGC 2 cut(s) 1538, 1696
Cac8I GCNNGC 2 cut(s) 569, 1067
CciI TCATGA 1 cut(s) 34
CfoI GCGC 1 cut(s) 1212
Cfr13I GGNCC 7 cut(s) 202, 203, 245, 1426, 1528, 1559, 1868
ClaI ATCGAT 2 cut(s) 1724, 1911
CseI GACGC 1 cut(s) 1786
Csp6I GTAC 6 cut(s) 191, 805, 880, 930, 1108, 1250
CviQI GTAC 6 cut(s) 191, 805, 880, 930, 1108, 1250
DdeI CTNAG 9 cut(s) 282, 576, 581, 630, 789, 864, 1242, 1764, 1773
DpnI GATC 2 cut(s) 265, 1896
DpnII GATC 2 cut(s) 263, 1894
DraI TTTAAA 1 cut(s) 549
DraIII CACNNNGTG 3 cut(s) 215, 821, 1124
EaeI YGGCCR 1 cut(s) 1330
Eam1104I CTCTTC 2 cut(s) 11, 824
EarI CTCTTC 2 cut(s) 11, 824
EciI GGCGGA 2 cut(s) 765, 1302
Eco130I CCWWGG 6 cut(s) 503, 933, 1158, 1402, 1429, 1573
Eco24I GRGCYC 1 cut(s) 206
Eco31I GGTCTC 1 cut(s) 1556
Eco47I GGWCC 4 cut(s) 245, 1528, 1559, 1868
Eco47III AGCGCT 1 cut(s) 1211
Eco57I CTGAAG 4 cut(s) 432, 639, 1038, 1617
Eco81I CCTNAGG 1 cut(s) 1773
Eco88I CYCGRG 2 cut(s) 554, 1756
EcoO109I RGGNCCY 1 cut(s) 1868
EcoRII CCWGG 8 cut(s) 94, 119, 461, 769, 919, 1306, 1481, 1561
EcoT14I CCWWGG 6 cut(s) 503, 933, 1158, 1402, 1429, 1573
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 6 cut(s) 503, 933, 1158, 1402, 1429, 1573
Esp3I CGTCTC 1 cut(s) 1779
FaqI GGGAC 3 cut(s) 349, 572, 1206
FblI GTMKAC 2 cut(s) 22, 133
Fnu4HI GCNGC 7 cut(s) 939, 1003, 1312, 1333, 1506, 1590, 1609
FokI GGATG 3 cut(s) 939, 1010, 1726
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 7 cut(s) 939, 1003, 1312, 1333, 1506, 1590, 1609
FspBI CTAG 1 cut(s) 504
GlaI GCGC 1 cut(s) 1211
GluI GCNGC 7 cut(s) 939, 1003, 1312, 1333, 1506, 1590, 1609
HaeII RGCGCY 1 cut(s) 1213
HaeIII GGCC 3 cut(s) 204, 1332, 1427
HapII CCGG 3 cut(s) 102, 1531, 1847
HgaI GACGC 1 cut(s) 1786
HhaI GCGC 1 cut(s) 1212
Hin1I GRCGYC 1 cut(s) 1797
Hin6I GCGC 1 cut(s) 1210
HinP1I GCGC 1 cut(s) 1210
HincII GTYRAC 2 cut(s) 154, 839
HindII GTYRAC 2 cut(s) 154, 839
HindIII AAGCTT 1 cut(s) 345
HinfI GANTC 5 cut(s) 305, 337, 620, 1664, 1857
HpaII CCGG 3 cut(s) 102, 1531, 1847
HphI GGTGA 2 cut(s) 80, 314
Hpy166II GTNNAC 8 cut(s) 23, 48, 134, 154, 839, 852, 1155, 1861
Hpy188I TCNGA 7 cut(s) 412, 631, 658, 968, 1654, 1765, 1899
Hpy188III TCNNGA 8 cut(s) 35, 170, 341, 375, 954, 1526, 1635, 1756
Hpy8I GTNNAC 8 cut(s) 23, 48, 134, 154, 839, 852, 1155, 1861
Hpy99I CGWCG 1 cut(s) 1802
HpyAV CCTTC 6 cut(s) 323, 603, 775, 1013, 1163, 1844
HpyCH4III ACNGT 7 cut(s) 158, 302, 355, 515, 536, 1388, 1752
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 577, 719, 1088, 1245, 1598
HpyF3I CTNAG 9 cut(s) 282, 576, 581, 630, 789, 864, 1242, 1764, 1773
Hsp92I GRCGYC 1 cut(s) 1797
HspAI GCGC 1 cut(s) 1210
Kzo9I GATC 2 cut(s) 263, 1894
LmnI GCTCC 8 cut(s) 104, 172, 505, 710, 1088, 1098, 1407, 1605
Lsp1109I GCAGC 5 cut(s) 925, 989, 1298, 1492, 1620
LweI GCATC 5 cut(s) 659, 731, 749, 1047, 1436
MaeI CTAG 1 cut(s) 504
MaeIII GTNAC 2 cut(s) 509, 598
MalI GATC 2 cut(s) 265, 1896
MboI GATC 2 cut(s) 263, 1894
MboII GAAGA 9 cut(s) 28, 399, 425, 450, 645, 841, 1620, 1623, 1725
MflI RGATCY 1 cut(s) 1894
MluCI AATT 8 cut(s) 142, 238, 369, 429, 666, 689, 1264, 1475
MlyI GAGTC 2 cut(s) 331, 1866
MseI TTAA 2 cut(s) 390, 548
MslI CAYNNNNRTG 4 cut(s) 66, 897, 1050, 1125
MspI CCGG 3 cut(s) 102, 1531, 1847
MvaI CCWGG 8 cut(s) 96, 121, 463, 771, 921, 1308, 1483, 1563
MwoI GCNNNNNNNGC 6 cut(s) 90, 577, 719, 1088, 1245, 1598
NciI CCSGG 2 cut(s) 1532, 1847
NcoI CCATGG 2 cut(s) 1429, 1573
NdeII GATC 2 cut(s) 263, 1894
NlaIV GGNNCC 6 cut(s) 100, 204, 247, 1165, 1639, 1716
NmuCI GTSAC 1 cut(s) 509
OliI CACNNNNGTG 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 1756
PagI TCATGA 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 1794
PfeI GAWTC 3 cut(s) 305, 620, 1664
PflFI GACNNNGTC 1 cut(s) 515
PfoI TCCNGGA 2 cut(s) 461, 1845
PkrI GCNGC 7 cut(s) 940, 1004, 1313, 1334, 1507, 1591, 1610
PleI GAGTC 2 cut(s) 331, 1865
PpsI GAGTC 2 cut(s) 331, 1865
PpuMI RGGWCCY 1 cut(s) 1868
Psp5II RGGWCCY 1 cut(s) 1868
Psp6I CCWGG 8 cut(s) 94, 119, 461, 769, 919, 1306, 1481, 1561
PspGI CCWGG 8 cut(s) 94, 119, 461, 769, 919, 1306, 1481, 1561
PspN4I GGNNCC 6 cut(s) 100, 204, 247, 1165, 1639, 1716
PspOMI GGGCCC 1 cut(s) 202
PspPI GGNCC 7 cut(s) 202, 203, 245, 1426, 1528, 1559, 1868
PspPPI RGGWCCY 1 cut(s) 1868
PstI CTGCAG 1 cut(s) 1603
PsuI RGATCY 1 cut(s) 1894
PsyI GACNNNGTC 1 cut(s) 515
RsaI GTAC 6 cut(s) 192, 806, 881, 931, 1109, 1251
RsaNI GTAC 6 cut(s) 191, 805, 880, 930, 1108, 1250
RseI CAYNNNNRTG 4 cut(s) 66, 897, 1050, 1125
SaqAI TTAA 2 cut(s) 390, 548
SatI GCNGC 7 cut(s) 939, 1003, 1312, 1333, 1506, 1590, 1609
Sau3AI GATC 2 cut(s) 263, 1894
Sau96I GGNCC 7 cut(s) 202, 203, 245, 1426, 1528, 1559, 1868
ScaI AGTACT 1 cut(s) 881
SchI GAGTC 2 cut(s) 331, 1866
SfaNI GCATC 5 cut(s) 659, 731, 749, 1047, 1436
SfcI CTRYAG 3 cut(s) 642, 700, 1599
Sfr274I CTCGAG 1 cut(s) 1756
SinI GGWCC 4 cut(s) 245, 1528, 1559, 1868
SlaI CTCGAG 1 cut(s) 1756
SmiMI CAYNNNNRTG 4 cut(s) 66, 897, 1050, 1125
SmlI CTYRAG 1 cut(s) 1756
SmoI CTYRAG 1 cut(s) 1756
Sse9I AATT 8 cut(s) 142, 238, 369, 429, 666, 689, 1264, 1475
SsiI CCGC 4 cut(s) 750, 1287, 1333, 1589
SspI AATATT 1 cut(s) 394
SspMI CTAG 1 cut(s) 504
StyI CCWWGG 6 cut(s) 503, 933, 1158, 1402, 1429, 1573
TaaI ACNGT 7 cut(s) 158, 302, 355, 515, 536, 1388, 1752
TaqI TCGA 3 cut(s) 1724, 1757, 1911
TaqII GACCGA 1 cut(s) 311
TasI AATT 8 cut(s) 142, 238, 369, 429, 666, 689, 1264, 1475
TatI WGTACW 2 cut(s) 879, 1107
TauI GCSGC 2 cut(s) 1335, 1592
TfiI GAWTC 3 cut(s) 305, 620, 1664
Tru1I TTAA 2 cut(s) 390, 548
Tru9I TTAA 2 cut(s) 390, 548
TscAI CASTG 6 cut(s) 169, 277, 520, 1342, 1648, 1674
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 5 cut(s) 938, 1002, 1311, 1505, 1608
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 3 cut(s) 603, 1725, 1742
TspGWI ACGGA 1 cut(s) 1878
TspRI CASTG 6 cut(s) 169, 277, 520, 1342, 1648, 1674
Tth111I GACNNNGTC 1 cut(s) 515
VneI GTGCAC 2 cut(s) 850, 1153
VpaK11BI GGWCC 4 cut(s) 245, 1528, 1559, 1868
XapI RAATTY 2 cut(s) 689, 1475
XhoI CTCGAG 1 cut(s) 1756
XmaJI CCTAGG 1 cut(s) 503
XmiI GTMKAC 2 cut(s) 22, 133
XspI CTAG 1 cut(s) 504
ZrmI AGTACT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.